• 제목/요약/키워드: F/A-18E/F

검색결과 440건 처리시간 0.03초

Virulence genes and antimicrobial resistance of pathogenic Escherichia coli isolated from diarrheic weaned piglets in Korea

  • Do, Kyung-Hyo;Byun, Jae-Won;Lee, Wan-Kyu
    • Journal of Animal Science and Technology
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    • 제62권4호
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    • pp.543-552
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    • 2020
  • For efficient prevention and treatment of enteric colibacillosis, understanding about latest virulence factors and antimicrobial resistance of Escherichia coli is essentially needed. The aim of this study was to survey antimicrobial resistance and determine the prevalence of fimbriae and enterotoxin genes among 118 pathogenic E. coli isolates obtained from Korean pigs with diarrhea between 2016 and 2017. The genes for the toxins and adhesins were amplified by polymerase chain reaction (PCR). The susceptibility of the E. coli isolates to antimicrobials were tested using the standard Kirby-Bauer disk diffusion method. The most prevalent fimbrial antigen was F18 (40.7%), followed by F4 (16.9%), and the most prevalent combinations of toxin genes were Stx2e (21.2%), STb:EAST-1 (19.5%), and STa:STb (16.9%), respectively. Among the pathotypes, enterotoxigenic E. coli (ETEC) was the most predominant (67.8%), followed by Shiga-toxin producing E. coli (STEC, 23.7%). We confirmed high resistance rates to chloramphenicol (88.1%), tetracycline (86.4%), streptomycin (86.4%), and ampicillin (86.4%). And the majorities of isolates (90.7%) showed multi-drug resistance which means having resistance to 3 or more subclasses of antimicrobials. Results of this study can be a source of valuable data for investigating the epidemiology of and control measures for enteric colibacillosis in Korean piggeries.

High Preoperative Fibrinogen and Systemic Inflammation Response Index (F-SIRI) Predict Unfavorable Survival of Resectable Gastric Cancer Patients

  • Gao, Weiwei;Zhang, Fei;Ma, Tai;Hao, Jiqing
    • Journal of Gastric Cancer
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    • 제20권2호
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    • pp.202-211
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    • 2020
  • Purpose: This study was to investigate the prognostic significance of the preoperative fibrinogen and systemic inflammation response index (F-SIRI) in a Chinese cohort of resectable gastric cancer. Materials and Methods: Baseline characteristics, preoperative fibrinogen levels and peripheral neutrophil, monocyte, and lymphocyte counts were retrospectively reviewed in 240 patients who underwent radical gastrectomy. The optimal cut-off values for fibrinogen and SIRI were defined as 4.0 g/L and 1.2. Then patients with hyperfibrinogenemia (≥4.0 g/L) and high SIRI (≥1.2) were assigned with an F-SIRI of 2 (both of these hematological abnormalities), 1 (one of these abnormalities), and 0 (neither abnormality), respectively. The prognostic value was examined by univariate and multivariate survival analysis. Results: Preoperative F-SIRI was significantly correlated with tumor size, fibrinogen level, and adjuvant chemotherapy. Whereas there was no significant difference in age, gender, tumor location or other characteristics between groups. In addition, high preoperative F-SIRI was significantly associated with worse disease-free survival (DFS) (hazard ratio [HR], 2.299; 95% confidence interval [CI], 1.482-3.566; P<0.001) and overall survival (OS) (HR, 2.461; 95% CI, 1.584-3.824; P<0.001) by univariate survival analysis. Moreover, it remained an independent predictor for impaired DFS (HR, 2.023; 95% CI, 1.273-3.215; P=0.003) and OS (HR, 2.341; 95% CI, 1.480-3.705; P<0.001) in multivariate Cox regression analysis. Conclusions: Preoperative F-SIRI could serve as a significantly prognostic marker for long-term survival in Chinese patients who underwent radical gastrectomy.

벼멸구 저항성 계통선발을 위한 MAS의 선발효율 비교 (Comparison of Selection Efficiency between Marker-Assisted Selection and Phenotypic Selection for Development of Brown Planthopper Resistance Lines in Rice)

  • 김석만;손재근
    • 한국육종학회지
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    • 제40권1호
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    • pp.48-53
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    • 2008
  • 본 연구에서는 벼멸구 저항성 개통 육성과정 중에 수행한 PS와 MAS system간의 선발 효율을 정확성, 소요시간, 소요비용 등의 요인으로 비교 분석함으로써 MAS system의 선발 효율을 제시하고, 이를 이용하여 효과적인 MAS system을 구축하고 실용화하기 위한 방안을 제시하였다. DNA marker를 이용하여 벼멸구 저항성 개체를 선발하고 MAS 체제를 확립하기 위하여 '삼강/낙동' 조합의 $F_2$집단, RIL($F_6$), DH집단, 여교잡집단($BC_6F_5$)을 대상으로 PS와 marker 검정을 병행하여 두 검정 방법 간에 각 잡종집단 및 세대별 저항성과 감수성 계통(개체)의 비율을 비교한 바 95%이상 일치하였다. MAS 체제와 PS간의 선발효율을 비교한 바 MAS는 PS에 비해 약 32%의 비용이 절감되었고, 검정에 소요되는 기간도 기존의 PS에서는 30일인데 비해 MAS에서는 7일로 크게 단축되었다. 이 결과를 바탕으로 MAS에 의한 벼멸구 저항성 계통 선발 체계를 확립 할 수 있었다.

점박이응애 천적인 3종 이리응애의 발육 및 포식량 비교 (Development and Prey Consumption of Phytoseiid Mites, Amblyseius womersleyi, A. fallacis, and Typhlodromus occidentalis under controlled Environments)

  • 권기면;이영인;이순원;최경희
    • 한국응용곤충학회지
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    • 제37권1호
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    • pp.53-58
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    • 1998
  • 점박이응애(Tetranychus urticae Koch)를 먹이로 제공하여 3종 이리응애의 발육 및 포식량을 조사한결과는 다음과 같다. 20, 23, 25, $30\pm$0.5$^{\circ}C$에서 발육기간은 Amblyseius womerslryi Schica 각 11.5, 7.7, 6.7, 5.6일이었으며, Amblyseius fallacis Garman과 Typhlodromus occidentalis Nesbit는 약간 짧았으나 큰 차이는 없었으며, $30^{\circ}C$에서 $20^{\circ}C$보다 1/2이하로 짧았다. 이리응애의 발육임계온도와 유효적산 온도는 암.수간에 차이가 없었고, 암컷을 기준으로 볼 때 A. womersleyi가 각 $8.8^{\circ}C$, 111.6일도, A. fallacis는 각 $10.7^{\circ}C$, 86.0일때도, T. occidentalis는 각 $10.7^{\circ}C$, 94.1 일도였다. $25^{\circ}C$에서 3종이리응애 암성충의 평균수명은 A. womersleyi $18.2\pm$8.67일, A. fallacis $19.6\pm$7.81일, T. occidentalis $13.0\pm$5.66일이었고, 총산란수는 각 $34.3\pm$11.93, $39.8\pm$12.64, $23.6\pm$8.86개였다. 이리응애의 전발육기간 동안 점박이응애 난 포식량은 20, 23, 25, $30\pm$$0.5^{\circ}C$에서 A. womersleyi가 각 $9.1\pm$2.49, $9.7\pm$2.00, 9.7$\pm$2.61, $1.03\pm$2.33개로 온도별로 차이가 없었고, A. fallacis는 비슷하였으며, T. occidentalis는 2~5개 많은 경향이었다. 3종 이리응애 암성충의 점받이응애 나과 전약충에 대한 포식량은 온도별.먹이 태별로 별 차이가 없었다.

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Oxytocin 투여가 임신 Rat의 Steroid Hormones 및 Oxytocin Receptors 농도에 미치는 영향 (Effects of Exogenous Oxytocin on Steroid Hormones and Oxytocin Receptor Concentrations in Pregnant Rats)

  • 박용수;조현수;변명대
    • 한국가축번식학회지
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    • 제26권2호
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    • pp.183-192
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    • 2002
  • 본 연구는 임신 및 분만후 rat에 oxytocin을 투여후 혈장, 자궁 및 태반 조직에서 estradiol-17$\beta$, progesterone, prostaglandin F$_2$$_{\alpha}$ 및 prostaglandin E$_2$그리고 자궁 조직의 oxytocin 수용체의 함량 사이의 관계에 대하여 조사하였다. 임신 rat에 oxytocin 투여 후 혈장 estradiol-17$\beta$의 농도는 임신 말기에 서서히 증가하여 임신 22일에 최고를 기록하였고 분만 후 1일에는 임신 16일 수준으로 감소하였다. Progesterone 농도는 임신 18일부터 분만 후 1일에 유의성 있는(p<0.05) 감소를 보였다. Prostaglandin F$_2$$_{\alpha}$ 및 Prostaglandin E$_2$농도는 임신 14일에서 임신 22일에 공히 상승하여 임신 22일에 최고를 기록하였고 분만 후 1일에는 급격히 감소하였다. Estradiol-17$\beta$의 농도는 자궁 조직에서 임신 14일에서 20일까지 서서히 증가하다가 임신 22일에 급격한 증가를 기록하였고 분만 후 1일에는 급격히 감소하여 임신 14일 수준을 유지하였으며 progesterone 농도는 임신 16일에 상승을 보인 후 임신 20일까지 급격히 감소하였고 분만 후 1일까지 같은 수준을 유지하였다. Prostaglandin F$_2$$_{\alpha}$ 및 prostaglandin E$_2$농도는 자궁조직에서 임신 말기 전기간에 상승하여 임신 22일에 최고를 기록한 후 분만 후 1일에는 급격히 감소하여 임신 14일과 비교하여 70% 수준을 기록하였다. Prostaglandin F$_2$$_{\alpha}$ 농도는 태반 조직에서 임신 14일에서 임신 22일까지 지속적으로 증가하였고 분만 후 1일에는 급격히 감소하였으며 prostaglandin E$_2$의 농도는 임신 14일에서 20일에 증가한 후 분만 후 1일까지 감소하였다. Oxytocin 수용체의 농도는 자궁 조직에서 임신 20일에서 임신 22일 사이에 급격히 증가한 후 분만 후 1일까지 비슷한 수준을 기록하였다. 이상에서와 oxytocin투여에 의한 임신 rat에 있어서 progesterone 농도의 감소에 이은 estradiol-17 $\beta$, prostaglandin F$_2$$_{\alpha}$, prostaglandin E$_2$및 oxytocin 수용체 농도의 증가는 조기에 시작되었다.

염색체 분석에 의한 생쥐초기부의 성 판별에 관한 연구 (Study on the Sexing of Mouse Embryos by Chromosomal Analysis)

  • 신현동;김정익
    • 한국가축번식학회지
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    • 제10권1호
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    • pp.27-35
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    • 1986
  • As a preliminary experiment to establish the process on the sexing of mouse embryos by chromosomal analysis, present studies were carried out with inbred (ICR, C57BL) and F1 hybrid [(ICR${\times}$C57BL) = F1 ${\times}$ ICR] mice to investigate the blastomere numbers and mitotic indices (M.I.) to the developmental stage of embryos recovered, the optimum periods of anti-mitotic agent administration, the successful rates of sexing and sex-ratio. The results obtained were summarized as follows: 1. The blastomere numbers (mean${\pm}$S.E.) of the morula and blastocyst were 18${\pm}$0.4 and 54${\pm}$0.7, respectively. 2. Whereas the M.I. of F1 hybrid (16${\pm}$0.2%) was higher than that fo inbred ICR (15${\pm}$0.1%) and C57BL (12${\pm}$0.6%) in the different strains, the morula (7${\pm}$0.6%) was higher than that of blastocyst (6${\pm}$0.4%) in the case of embryo stages. 3. Following to anti-mitotic agents treated, the M.I. of embryos cultured with Colcemid (17${\pm}$1.1%) was superior to that fo embryos cultured with Velban (12${\pm}$0.9%) and the Colcemid injection (7${\pm}$0.4%). 4. The successful rate of sexing in the blastocyst (38.7%; 124/320) was superior to the morula (35.9%; 52/145), and the F1 hybrid (48.1%) was higher than that of inbred ICR (42.4%) and C57 BL (28.2%). 5. In the successful rate of sexing to the methods of administration, the embryos cultured with Colcemid (46.0%) was superior to that of embryos cultured with Velban (39.0%) and the Colcemid injection (38.8%). 6. Of 98 embryos sexed after culture with Colcemid, 89(90.8%) were observed between 2 and 4 hrs. In the case of Velban treatment, 83.1% (74/89) was observed between 2$\frac{1}{2}$ and 4$\frac{1}{2}$ hrs. 7. Out of 761 prepared embryos it was possible to sex 311; 157 were male and 154 were female, i.e.a sex-ratio of 50% a, pp.oximately.

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남편의 산후조리 인식, 교육요구도 및 가족건강성에 관한 연구 (Husbands' Awareness of Sanhujori, Needs for Education and Family Strength)

  • 주은경;유은광
    • 여성건강간호학회지
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    • 제21권2호
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    • pp.93-105
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    • 2015
  • Purpose: We conducted a descriptive study to: 1) understand how husbands are aware of the importance of Sanhujoiri(i.e, Korean traditional postpartum care) and 2) identify their needs to learn how to take care of postpartum women and newborns and to strengthen family bonding. Methods: The subjects consisted of 123 husbands who had children under the age of 5 years. Data were analyzed with t-test, one-way ANOVA, $Scheff{\acute{e}}^{\prime}s$ test, and Pearson's correlation. Results: The mean age of subjects was $37.1{\pm}4.63$ years. The mean scores of awareness, needs for education on postpartum mother and newborn care, and family strength were $4.57{\pm}1.18$, $4.61{\pm}1.18$, $4.92{\pm}1.07$, and $4.01{\pm}0.95$, respectively, indicating higher scores. There were statistically significant differences in awareness (F=5.08, p<.05), newborn care (F=3.70, p<.05), and family strength (F=4.64, p<.05) by husband's role in Sanhujori There was a positive correlation among study variables. Conclusion: This study shows that even though husbands want to participate in Sanhujori, they do not have enough information on Sanhujori and mother/newborn care and adequate paternity leave. Realistic paternity leave system and effective Couple Centered Childbearing (from pregnancy to postpartum) Education Program CCCEP development are required to help husbands' participation in mother/newborn care with confidence and competence in home based Sanhujori.

율동적 동작(Dance movement) 훈련이 노년기 여성의 생리, 심리적 변수에 미치는 영향 (Effect of Korean Traditional Dance Movement Training on Psychophysiological Variables in Korean Elderly Women)

  • 전미양;최명애
    • 대한간호학회지
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    • 제26권4호
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    • pp.833-852
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    • 1996
  • Regular long term dance movement could be one of ways to induce improvement of psychophysiological variables, resulting in improvement of quality of life. However, there have been few studies to evaluate the effect of dance movement training on both physiological and psychological variables in the elderly. This study was focused to determine the effect of Korean traditional dance movement training on psychophysiological variables-body weight, body fat, lean body mass, muscle strength, muscle endurance, flexibility, agility, resting heart rate and blood pressure, depression and life satisfaction-in Korean elderly women. Thirty four subjects, aged between 65 and 75years who have normal cognition, sensory function, cerebellum function, cardiovascular function, participated in this study. Seventeen experimental group subjects were selected from E-elderly university in Kyung Gi province, and Seventeen control group subjects were selected from N-welfare facility in Seoul City. Seventeen experimental group subjects participated for 12weeks dance movement program. Korean traditional dance movement program was developed on the basis of Korean traditional dance and music by the author. The program consisted of approximately 50minutes of dance, 3times a week for 12weeks. During 50minutes workout, there were 15minutes of warm-up dancing, 25minutes of conditioning dance and 10minutes of cool-down dancing. The intensity for the conditioning phase was at between 60% and 65% of age-adjusted maximum heart rates. The body weight, body fat, lean body mass, muscle strength(grip strength, leg strength), muscle endurance, flexibility, agility, resting heart rate and blood pressure, depression and life satis-faction were measured prior to and following the experimental treatment. The participants in dance movement were interviewed focusing on subjective feeling following 12 week's regular dance movement. Data were analyzed with mean, standard deviation, percentage of change, X²-test, t-test, and ANCOVA test using SPSS PC/sup +/ program. Subjective feeling was categorized into cognitopsy-chological and physiological responses. Results were obtained as follows : 1) The body weight (F=15.52, p=.000), body fat (F=18.33, p=.000) and lean body mass (F=7.28, p=.011) of the experimental group were significantly lower than those of the control group following the dance movement training. 2) The leg strength (F=30.96, p=.000), muscle endurance (F=9.06, p=.005), agility(F=44.92, 000), flexibility(F=6.84, p=.014) of the experimental group were significantly higher than those of the control group following the dance movement training. There was no significant difference of grip strength(F=.43, p=.515) between experimental and control groups. 3) The heart rate(F=26.96, p=.000), systolic (F=10.40, p=.000) and diastolic(F=3.99, p=.005) blood pressure at rest of the experimental group were significantly lower than those of the control group following the dance movement training. 4) No significant difference of score of depression (F=3.49, p=.071) was observed between experimental and control groups. 5) Score of life satisfaction of experimental group was remarkably higher than that of control group following 12weeks of dance movement training (p<0.05). 6) Thematic responses about the dance movement following the training were positive. "I feel good" was the most frequent among cognitopsychological responses and "I feel lightness of body" was the most frequent among physiological responses. The results suggest that Korean traditional dance movement training can improve psychophysiological variables of Korean elderly.

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쌍끌이 중층트롤어법의 연구 ( 1 ) - 모형어구의 망구형상에 관하여 - ( A Study on the Pair Midwater Trawling ( 1 ) - Mouth Performance of the Model Net - )

  • 이병기
    • 수산해양기술연구
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    • 제31권1호
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    • pp.29-44
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    • 1995
  • A model experiment on the pair midwater trawl net applicable to 800 PS class Korean pair bottom trawlers was carried out in the special-prepared experimental thank. the tank was prepared as a reverse trapezoid shape in its vertical section by digging out flat soil. The dimension of the tank showed the 9.6 W$\times$43.0 L(m) of the upper fringe and the 4.8 W$\times$38.0 L(m) of the bottom with 3.0m in depth. The depth of water was maintained 2.7m during experiment. The model net was prepared based on the Tauti's similarity law of fishing gear in 1/30 scale considering the dimension of the experimental tank. Mouth performance of the model net during towing were determined by the photographs taken in front of the net mouth with the combinations of towing velocity, warp length and distance between paired boats. The results obtained can be summarized as follows: 1. Vertical opening of the model nets A and B was varied in the range of 0.18~0.88 m and 0.21~0.78 m (which can be converted into 5.4~26.4m and 6.3~23.4 m in the full-scale net) respectively, and was varied predominantly by towing speed. Vertical opening (H which is appendixed m for the model net. f for the full-scale net. A and B for the types of the model net) can be expressed as the function of towing velocity$V_t$as in the model net $V_t$ : m/ sec)$H_{mA}$=1.67$e^{-1.65V_t}$ $H_{mB}$=1.15$e^{-1.13V_t}$, in the full-scale net ($V_t$ : k't) $H_{fA}$=50.27$e^-0.37V_t$ $H_{fB}$=34.46$e^{-0.26Vt}$. 2. Horizontal opening of the model nets An and b was varied in the range of 1.03~1.54m and 1.04~1.55 m (which can be converted into 30.9~46.2 m and 31.2~46.5m in the full-scale net) respectively, and was varied predominantly by distance between paired boats. Horizontal opening (W, appendixes are as same as the former) an be expressed as the function of distance between paired boats $D_b$as in the model net $W_{mA}$=0.69+0.09$D_b$ $W{mB}$=0.73+0.09$D_b$, in the full-scale net $W_{fA}$=20.81+0.09$D_b$ $W_{fB}$=22.11+0.09$D_b$ 3. Net opening area of the model net A and B was varied in the range of 0.28~1.04 $m^2$ and 0.33~0.94$m^2$(which can be converted into 252~936$m^2$ and 297~846$m^2$ in the full-scale net) respectively, and was varied predominantly by towing velocity. Net opening area ($S$, appendixes are as same as the former) van be expressed as the function of towing velocity$V_t$ as in the model net $v_t$ : m/sec) $S_{Ma}$=2.01$e^{-1.54V_T}$ $S_{mA}$=1.40$e^{-1.65V_t}$, in the full-scale net ($V_t$ : k't) $S_{fA}$=1.807$e^-0.35V_t$ $S_{fA}$=1.265$e^{-0.24V_t}$. 4. Filtering volume of the model nets A and B was varied in the range of 0.32~0.55 $m^3$ and 0.37~0.55$m^3$(which can be converted into 8.640~14.850 $m^3$ and 9.990~14.850$m3$in the full~scale net) respectively, and was predominantly varied by towing speed. filtering volume of the model net-A showed the maximum at the towing speed 0.69 m/sec(3 k't in the full-scale net), compared with that of the model net B showed at 0.92 m/sec(4 k't in the full-scale net).

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