• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.378-387
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• 1998

The DNA sequence immediately following the xynA gene of Bacillus stearothermophilus 236 ［about l-kb region downstream from the translational termination codon (TAA) of the xynA gene］was found to have an ability to enhance the xylanase activity of the upstream xynA gene. An 849-bp ORF was identified in the downstream region, and the ORF was confirmed to encode a novel protein of 283 amino acids designated as XaiF (xylanase-activity-increasing factor). From the nucleotide sequence of the xaiF gene, the molecular mass and pI of XaiF were deduced to be 32,006 Da and 4.46, respectively. XaiF was overproduced in the E. coli cells from the cloned xaiF gene by using the T7 expression system. The transcriptional initiation site was determined by primer extension analysis and the putative promoter and ribosome binding regions were also identified. Blast search showed that the xaiF and its protein product had no homology with any gene nor any protein reported so far. Also, in B. subtilis, the xaiF trans-activated the xylanase activity at the same rate as in E. coli. In contrast, xaiF had no activating effect on the co-expressed ${\beta}-xylosidase$ of the xylA gene derived from the same strain of B. stearothermophilus. In addition, the intracellular and extracellular fractions from the E. coli cells carrying the plasmid-borne xaiF gene did not increase the isolated xylanase activity, indicating that the protein-protein interaction between XynA and XaiF was not a causative event for the xylanase activating effect of the xaiF gene.

• Journal of Yeungnam Medical Science
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• 제35권1호
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• pp.1-6
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• 2018

Alzheimer's disease (AD) is a neurodegenerative disorder associated with extracellular plaques, composed of amyloid-beta ($A{\beta}$), in the brain. Although the precise mechanism underlying the neurotoxicity of $A{\beta}$ has not been established, $A{\beta}$ accumulation is the primary event in a cascade of events that lead to neurofibrillary degeneration and dementia. In particular, the $A{\beta}$ burden, as assessed by neuroimaging, has proved to be an excellent predictive biomarker. Positron emission tomography, using ligands such as $^{11}C$-labeled Pittsburgh Compound B or $^{18}F$-labeled tracers, such as $^{18}F$-florbetaben, $^{18}F$-florbetapir, and $^{18}F$-flutemetamol, which bind to $A{\beta}$ deposits in the brain, has been a valuable technique for visualizing and quantifying the deposition of $A{\beta}$ throughout the brain in living subjects. $A{\beta}$ imaging has very high sensitivity for detecting AD pathology. In addition, it can predict the progression from mild cognitive impairment to AD, and contribute to the development of disease-specific therapies.

• IMMUNE NETWORK
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• 제4권4호
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• pp.216-223
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• 2004

Background: It is well known that IgA isotype switching is induced by $TGF-{\beta}1$. LPS-activated mouse normal B cells well differentiate into IgA secreting plasma cells under the influence of $TGF-{\beta}1$. Nevertheless, there are lots of difficulties in studying normal B cells in detail because it is not simple to obtain highly purified B cells, showing low reproducibility and transfection efficacy, moreover impossible to keep continuous culture. To overcome these obstacles, it is desperately needed to develop B cell line which acts like normal B cells. In the present study, we investigated whether CH12F3-2A lymphoma cells are appropriate for studying IgA isotype switching event. Methods: CH12F3-2A B cell line was treated with LPS and $TGF-{\beta}1$, then levels of germ-line (GL) transcripts were measured by RT-PCR, and $GL{\alpha}$ promoter activity was measured by luciferase assay. In addition, membrane IgA (mIgA) expression and IgA secretion were determined by FACS and ELISA, respectively. Results: $TGF-{\beta}1$, regardless of the presence of LPS, increased level of $GL{\alpha}$ transcripts but not $GL{\gamma}2b$ transcripts. However, IgA secretion was increased dramatically by co-stimulation of LPS and $TGF-{\beta}1$. Both mIgA and IgA secretion in the presence of $TGF-{\beta}1$ were further increased by over-expression of Smad3/4. Finally, $GL{\alpha}$ promoter activity was increased by $TGF-{\beta}1$. Conclusion: CH12F3-2A cell line acts quite similarly to the normal B cells which have been previously reported regarding IgA expression. Thus, CH12F3-2A lymphoma cell line appears to be adequate for the investigation of the mechanism(s) of IgA isotype switching at the cellular and molecular levels.

• Molecules and Cells
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• 제25권2호
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• pp.205-210
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• 2008

To develop molecular markers linked to the $L^4$ locus conferring resistance to tobamovirus pathotypes in pepper plants, we performed AFLP with 512 primer combinations for susceptible (S pool) and resistant (R pool) DNA bulks against pathotype 1.2 of pepper mild mottle virus. Each bulk was made by pooling the DNA of five homozygous individuals from a T10 population, which was a near-isogenic $BC_4F_2$ generation for the $L^4$ locus. A total of 19 primer pairs produced scorable bands in the R pool. Further screening with these primer pairs was done on DNA bulks from T102, a $BC_{10}F_2$ derived from T10 by back crossing. Three AFLP markers were finally selected and designated L4-a, L4-b and L4-c. L4-a and L4-c each underwent one recombination event, whereas no recombination for L4-b was seen in 20 individuals of each DNA bulk. Linkage analysis of these markers in 112 $F_2$ T102 individuals showed that they were each within 2.5 cM of the $L^4$ locus. L4-b was successfully converted into a simple 340-bp SCAR marker, designated L4SC340, which mapped 1.8 cM from the $L^4$ locus in T102 and 0.9 cM in another $BC_{10}F_2$ population, T101. We believe that this newly characterized marker will improve selection of tobamovirus resistance in pepper plants by reducing breeding cost and time.

• Asian-Australasian Journal of Animal Sciences
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• 제31권9호
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• pp.1393-1400
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• 2018

Objective: This study was carried out to assess the haplotype diversity and population dynamics in cattle populations of Ethiopia. Methods: We sequenced the complete mitochondrial cytochrome b gene of 76 animals from five indigenous and one Holstein Friesian${\times}$Barka cross bred cattle populations. Results: In the sequence analysis, 18 haplotypes were generated from 18 segregating sites and the average haplotype and nucleotide diversities were $0.7540{\pm}0.043$ and $0.0010{\pm}0.000$, respectively. The population differentiation analysis shows a weak population structure (4.55%) among the populations studied. Majority of the variation (95.45%) is observed by within populations. The overall average pair-wise distance ($F_{ST}$) was 0.049539 with the highest ($F_{ST}=0.1245$) and the lowest ($F_{ST}=0.011$) $F_{ST}$ distances observed between Boran and Abigar, and Sheko and Abigar from the indigenous cattle, respectively. The phylogenetic network analysis revealed that all the haplotypes detected clustered together with the Bos taurus cattle and converged to a haplogroup. No haplotype in Ethiopian cattle was observed clustered with the reference Bos indicus group. The mismatch distribution analysis indicates a single population expansion event among the cattle populations. Conclusion: Overall, high haplotype variability was observed among Ethiopian cattle populations and they share a common ancestor with Bos taurus.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.119-121
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• 1999

Tumor cell interaction with the extracellular matrix is defined as the critical event of tumor invasion that signals the initiation of a metastatic cascade. Sesquicillin has been identified as an inhibitor of melanoma cell adhesion to the components of the extracellular matrix (ECM) in cultured broth of fungal strain F60063. Sesquicillin strongly inhibited the adhesion of B16 melanoma cells to laminin, fibronectin, and typeIV collagen. It also inhibited B16 melanoma cell invasion of reconstituted basement membrane Matrigel in vitro in a dose-dependent manner. These results suggest that sesquicillin is a new class of nonpeptidic ECM adhesion inhibitor having anti-invasive activity.

• 한국음향학회지
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• 제39권5호
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• pp.414-423
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• 2020

본 논문은 약한 레이블 기반 음향 이벤트 검출을 위한 시간-주파수 영역분할 맵 추출 모델에서 발생하는 희소성 및 수용영역 부족에 관한 문제를 완화 시키기 위해, 확장 게이트 선형 유닛(Dilated Convolution Gated Linear Unit, DCGLU)을 제안한다. 딥러닝 분야에서 음향 이벤트 검출을 위한 영역분할 맵 추출 기반 방법은 잡음 환경에서 좋은 성능을 보여준다. 하지만, 이 방법은 영역분할 맵을 추출하기 위해 특징 맵의 크기를 유지해야 하므로 풀링 연산 없이 모델을 구성하게 된다. 이로 인해 이 방법은 희소성과 수용영역의 부족으로 성능 저하를 보이게 된다. 이런 문제를 완화하기 위해, 본 논문에서는 정보의 흐름을 제어할 수 있는 게이트 선형 유닛과 추가의 파라미터 없이 수용영역을 넓혀 줄 수 있는 확장 합성곱 신경망을 적용하였다. 실험을 위해 사용된 데이터는 URBAN-SED와 자체 제작한 조류 울음소리 데이터이며, 제안하는 DCGLU 모델이 기존 베이스라인 논문들보다 더 좋을 성능을 보였다. 특히, DCGLU 모델이 자연 소리가 섞인 환경인 세 개의 Signal to Noise Ratio(SNR)(20 dB, 10 dB, 0 dB)에서 강인하다는 것을 확인하였다.

• Journal of Astronomy and Space Sciences
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• 제25권1호
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• pp.33-42
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• 2008

행성간 자기장(Interplanetary Magnetic Field)이 남쪽방향으로 전환되면서 발생된 2003년 자기폭풍 동안 한반도 상공 전리층의 총 전자수 함유량(Total Electron Content, TEC) 변화에 대해 알아보았다. 남쪽방향의 행성간 자기장과 지구 자기장의 상호작용에 의해 고위도에서 유도된 전기장과 저위도 전리층으로 전파된 전기장이 저위도 지역의 TEC 증가와 관련 있는 것으로 생각된다. 데이터 분석결과 2003년 6월 16일에 발생한 이벤트 동안에는 낮 지역 TEC 값이 약 15% 증가하였다. F2층의 최대 높이를 나타내는 hmF2는 300km 까지 상승되었으며, 수직방향 $E{\times}B$ 표류운동은 아랫방향으로 나타났다. 이것은 남쪽방향의 행성간 자기장 동안 고위도 지역으로 유입된 에너지에 의해 발생한 전리층의 교란된 다이나모 전기장이 TEC을 증가시킨 것으로 추정된다. 그러나 11월 20일에 발생한 이벤트 동안에는 전리층으로 전파된 서쪽방향 전기장에 의해 밤 지역 TEC 갈이 약 10% 증가한 것으로 보여 진다. 행성간 자기장이 남쪽방향으로 전환됨과 동시에 hmF2 높이는 200km까지 감소되었으며, 아랫방향 $E{\times}B$ 표류운동이 나타났다. 또한 행성간 전기장 y성분과 수직방향 TEC 값이 거의 비슷하게 변화하는 것을 볼 수 있었다. 이러한 결과들은 서로 다른 원인에 의해 발생하였지만 전리층의 교란된 전기장이 한반도 상공의 순간적인 TEC 값 증가에 중요한 요인으로 작용함을 보여 준다.

• 한국조리학회지
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• 제14권2호
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• pp.46-60
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• 2008

In the hotel industry, there are the production and consumption of services at the same time, considering direct contact between employees and customers. In this respect, this study was conducted based on more practical factors to grasp employees' feelings and conflicts. The results of this research are as follows. First, individual conflicts among hotel banquet employees influence devotion to the organization which has a direct effect on performance of management. Second, such devotion is closely correlated with the factors of the performance of management. Finally, managing employees' conflicts is the key factor to promote the performance of management. Under these circumstances, there should be more concern in solving problems among employees and special pro-grams for promoting job satisfaction and devotion which bring about more profits as well as a sense of achievement.

• 생약학회지
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• 제31권4호
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• pp.394-400
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• 2000

Tumor cell interaction with the extracellular matrix (ECM) is defined as the critical event of tumor invasion that signals the initiation of a metastatic cascade. To search for anti-metastatic agent from plants, several plant extracts were screened by cell- ECM anti-adhesion test. As result, Boehmeria pannosa, Dryopteris crassirhizoma, Scilla scilloides, and Agrimonia pilosa were shown a significant anti-adhesion activity.