• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2006.05a
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• pp.78-85
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• 2006

In textile industries, waste effluent containing zinc is generated during the manufacture of rayon yarn from the wood pulp or cotton linters. Due to the strict environmental regulations and the presence of toxic metallic and other constituents, the discharge of industrial effluents in the sewage or disposal of solid sludge as landfill is restricted. Before recycling of zinc as zinc sulphate solution to the spinning-bath of the rayon manufacturing plant the zinc sulphate solution must be free from calcium, which is deleterious to the process as gypsum precipitates with the increase in concentration and forms scale in the bath. In the present work an attempt has been made to develop a process following solvent extraction technique using thiophosphinic extractants, Cyanex 272 and 302 modified with isodecanol and diluted in kerosene to recover zinc from rayon effluent. Various process parameters viz. extraction of zinc from different concentration of solution, distribution ratio, selective extraction, O/A ratio on extraction and stripping from the loaded organic, complex formation in the organic phase etc. have been studied to see the feasibility of the process. The extractant Cyanex 302 has been found selective for the recovery of 99.99% of zinc from the effluent above equilibrium pH 3.4 maintaining the O/A ratio of 1/30 leaving all the calcium in the raffinate. It selectively extracted zinc in the form of complex $[R_{2}Zn.3RH]_{org}$ and retained all the calcium in the aqueous raffinate. The zinc from the loaded Cyanex 302 can be stripped with 10% sulphuric acid at even O/A ratio of 10 without affecting the stripping efficiency. The stripped solution thus obtained could be recycled in the spinning bath of the rayon plant. The raffinate obtained after the recovery of zinc could be disposed safely without affacting environment.

• Journal of Plant Biology
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• v.30 no.3
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• pp.201-203
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• 1987

Total RNA was isolated from two months old wheat, rice, tobacco and sweet potato. The procedure used was simple and provided pure RNA preparation. Lysis of plant tissue in a buffer with guanidine thiocyanate and CsCl density gradient centrifugation separated RNA from the rest of the cellular components. Subsequent cholroform/1-butanol extraction and ethanol precipitation were necessary to ensure contaminant-free RNA preparation. Storage of the lysed plant tissue in the buffer with guanidine thiocyanate preserved the sample for two months without noticeable RNA degradation.

• Natural Product Sciences
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• v.11 no.3
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• pp.123-126
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• 2005

Gastrodia elata Blume (Chunma) belongs to Orchidaceae, which is a perennial parasitic herbaceous plant and grows in the woods of the central provinces of China, Korea and Japan. Recently, the constituents of the tubers of this plant have been investigated by researchers who have revealed the presence of phenolic compounds including gastrodin as a major constituent, together with 4-hydroxybenzaldehyde, 4-hydroxybenzyl alcohol, parishin, 4,4-dihydroxybenzyl sulfoxide, vanillin, vanillyl alcohol, beta-sitosterol, organic acids and polysaccharides, etc. In this study, we used ultrasonicator and two kinds of enzymes for enhancement of extraction yield. We also used electronic nose for the aroma pattern analysis of Chunma extracts. The concentrations of glucose and functional constituents (gastrodin, vanillin, 4-hydroxybenzaldehyde and 4-hydroxybenzyl alcohol) were measured by biochemistry analyzer and HPLC, respectively. Therefore, we showed that the yield of extraction was increased and discomfortable odor was reduced.

• Bulletin of the Korean Chemical Society
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• v.32 no.12
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• pp.4275-4280
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• 2011

Present study describes the optimization of disk type sorptive extraction using monolithic material (Mono Trap) for the analysis of volatile aroma compounds from Lantana camara L. in combination with gas chromatography/mass spectrometry (GC/MS). Monolithic material sorptive extraction (MMSE) is a new sampling technique using a monolithic hybrid adsorptive disk (O.D. 10 mm, 1 mm thickness) made of high purity silica and activated carbon having a large surface area chemically bonded with octadecyl silane (ODS). The experimental parameters that may influence the MMSE efficiency have been optimized. Linearity, accuracy, precision and detection limits were evaluated to assess the performance of the proposed method. The method was validated with real plant samples of Lantana camara L. Twenty eight compounds including the main representative compounds of ${\alpha}$-curcumene and ${\beta}$-caryophyllene were found in analyzed samples. Results proved that proposed method could be used as a good alternative for the analysis for such volatile aroma compounds in plant samples.

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.283-297
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• 2000

Seeds are an ideal repository for recombinant proteins in molecular farming applications. However, in order to use plant seeds efficiently for the production of such proteins, it is necessary to understand a number of fundamental biological properties of seeds. This includes a full understanding of promoters which function in a seed-specific manner, the subcellular targeting of the desired polypeptide and the final form in which a protein is stored. Once a biologically active protein has been deposited in a seed, it is also critical that the protein can be extracted and purified efficiently. In this review, these issues are examined critically to provide a number of approaches which may be adopted for production of recombinant proteins in plants. Particular attention is paid to the relationship between subcellular localization and protein extraction and purification. The robustness and flexibility of seed-based production is illustrated by examples close to or already in commercial production.

• The Plant Pathology Journal
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• v.26 no.3
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• pp.289-292
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• 2010

This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.546-551
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• 2009

We examined the growth characteristics, fresh tuber yield, rotenone content of two yam beans (Pachyrhizus erosus and P. ahipa) introduced from Mexico. P. erosus species showed better adaptable ability than P. ahipa species in seed productivity and tuber yield. Rotenone content in the P. erosus species extracted with chloroform was 3.6 folds much more extracted than ethanol extraction. The order of rotenone content found in crude extract obtained by different solvent extraction from the highest to the lowest was mature seed (484.7 ${\mu}g$) and leaves (17.2 ${\mu}g$) of the P. erosus species, respectively.

• Advances in Energy Research
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• v.8 no.3
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• pp.185-202
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• 2022

This research work focuses to design and simulate a 200W solar power system with electrical power conservation scheme as well as thermal power conservation modeling to improve power extraction from solar power plant. Many researchers have been already designed and developed different methods to extract maximum power while there were very researches are available on improving solar power thermally and mechanically. Thermal parameters are also important while discussing about maximizing power extraction of any power plant. A specific type of coolant which have very high boiling point is proposed to be use at the bottom surface of solar panel to reduce the temperature of panel in summer. A comparison between different maximum power point tracking (MPPT) technique and proposed MPPT technique is performed. Using this proposed Thermo-electrical MPPT (TE-MPPT) with Deep Learning Algorithm model 40% power is conserved as compared to traditional solar power system models.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.150.3-151
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• 2003

To analyze the genome of Cucumber mosaic virus(CMV) in pepper, we developed a new extraction method for double-stranded RNA(dsRNA). To isolate the dsRNA, 0.1g of pepper leaves homogenized with 1ml of 5${\times}$EXB extraction buffer[0.5M glycin, 0.5M NaCl, 5mM EDTA(pH9.0/NaOH), 10％ Sodium N-lauryl salcosinate(NLS), 10％ Sodium dodecylsulfate(SDS)] and purified with the 1/4 volume of phenol： chloroform： isoamylalcohol(25：24：1). dsRNAs from the aqueous phase was precipitated with isopropanol. This procedure was able to detect a minimal amount of dsRNA from CMV infected plant tissue and to distinguish different CMV satellite RNAs by polyacrylamide gel electrophoresis(PAGE). Moreover, this method can be applied CMV infected in pepper or Rice dwarf virus (RDV) infected rice.

• Journal of Radiation Protection and Research
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• v.41 no.2
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• pp.117-121
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• 2016

Background: Definition and grouping of initiating events (IEs) are important basics for probabilistic safety assessment (PSA). An IE in a spent fuel reprocessing plant (SFRP) is an event that probably leads to the release of dangerous material to jeopardize workers, public and environment. The main difference between SFRPs and nuclear power plants (NPPs) is that hazard materials spread diffusely in a SFRP and radioactive material is just one kind of hazard material. Materials and Methods: Since the research on IEs for NPPs is in-depth around the world, there are several general methods to identify IEs: reference of lists in existence, review of experience feedback, qualitative analysis method, and deductive analysis method. While failure mode and effect analysis (FMEA) is an important qualitative analysis method, master logic diagram (MLD) method is the deductive analysis method. IE identification in SFRPs should be consulted with the experience of NPPs, however the differences between SFRPs and NPPs should be considered seriously. Results and Discussion: The plutonium uranium reduction extraction (Purex) process is adopted by the technics in a model reprocessing plant. The first extraction cycle (FEC) is the pivotal process in the Purex process. Whether the FEC can function safely and steadily would directly influence the production process of the whole plant-production quality. Important facilities of the FEC are installed in the equipment cells (ECs). In this work, IEs in the FEC process were identified and categorized by FMEA and MLD two methods, based on the fact that ECs are containments in the plant. Conclusion: The results show that only two ECs in the FEC do not need to be concerned particularly with safety problems, and criticality, fire and red oil explosion are IEs which should be emphatically analyzed. The results are accordant with the references.