Enhanced Yield of Extraction from Gastrodia elata Blume by Ultrasonication and Enzyme Reaction

  • Kim, Hyun-Jong (Division of Environmental and Chemical Engineering, Chonbuk National University) ;
  • Kwak, In-Seob (Division of Environmental and Chemical Engineering, Chonbuk National University) ;
  • Lee, Bong-Soo (Division of Environmental and Chemical Engineering, Chonbuk National University) ;
  • Oh, Seung-Bae (Division of Environmental and Chemical Engineering, Chonbuk National University) ;
  • Lee, Hyun-Chul (Department of Advanced Material Engineering, Hanlyo University) ;
  • Lee, Eun-Mi (Jeonbuk Bioindustry Development Institute) ;
  • Lim, Ja-Young (Mu-Ju Health Food Association) ;
  • Yun, Yeoung-Sang (Division of Environmental and Chemical Engineering, Chonbuk National University) ;
  • Chung, Bong-Woo (Division of Environmental and Chemical Engineering, Chonbuk National University)
  • Published : 2005.09.01

Abstract

Gastrodia elata Blume (Chunma) belongs to Orchidaceae, which is a perennial parasitic herbaceous plant and grows in the woods of the central provinces of China, Korea and Japan. Recently, the constituents of the tubers of this plant have been investigated by researchers who have revealed the presence of phenolic compounds including gastrodin as a major constituent, together with 4-hydroxybenzaldehyde, 4-hydroxybenzyl alcohol, parishin, 4,4-dihydroxybenzyl sulfoxide, vanillin, vanillyl alcohol, beta-sitosterol, organic acids and polysaccharides, etc. In this study, we used ultrasonicator and two kinds of enzymes for enhancement of extraction yield. We also used electronic nose for the aroma pattern analysis of Chunma extracts. The concentrations of glucose and functional constituents (gastrodin, vanillin, 4-hydroxybenzaldehyde and 4-hydroxybenzyl alcohol) were measured by biochemistry analyzer and HPLC, respectively. Therefore, we showed that the yield of extraction was increased and discomfortable odor was reduced.

Keywords

References

  1. Chung, H.S. and Ji, G.E., Composition and Functionality of Chunma. Korean J. Food Sci. Technol. 28, 53-57 (1996)
  2. Hayashi, J, Sekine, T., Deguch, S., Lin, Q., Horie, S., Tsuchiya, S., Yano, S., Watanabe, K., and Ikegani, F., Phenolic compounds from Gastrodia rhizome and relaxant effects of related compounds on isolated smooth muscle preparation. Phytochemistry 59, 513-519 (2002) https://doi.org/10.1016/S0031-9422(02)00008-0
  3. Hsieh, M.T., Wu, C.R., and Chen, C.F., Gastrodin and p-hydroxybenzyl alcohol facilitate memory consolidition and retrieval but not acquisition on the passive avoidance task in rats. J. Ethnopharmacol. 56, 45-54 (1997) https://doi.org/10.1016/S0378-8741(96)01501-2
  4. Kang, T.S., Kong, Y.J., Kwon, H.J., Choi, B.K., Hong, J.G., and Park, Y.K., A Studies on the Chemical Composition and in vitro Biological Activities of a Hot Water Extracts of Gastrodia elata. Korean J. Mycol. 30, 136-141 (2002) https://doi.org/10.4489/KJM.2002.30.2.136
  5. Ku, B.H., Experimental studies on the pharmaceutical effects of Gastrodia elata, M.S. thesis, Kyung-Hee Univ., Seoul, Korea, 1991
  6. Lee, B.Y., Choi, H.S., and Hwang, J.B., Analysis of Food Components of Gastrodiae Rhizoma and Changes in Several Characteristics at the Various Drying Conditions. Korean J. Food Sci. Technol. 34, 37-42 (2002)
  7. Lee, B.Y., Yang, Y.M., and Han, C.K., Analysis of Aroma Pattern of Gastrodiae Rhizoma by the Drying Conditions. Korean J. Food Sci. Technol. 34, 13-17 (2002)
  8. Lee, J.W. and Kim, Y.K., Volatile Flavor Constituents in the Rhizoma of Gastrodia elata. Agri. Chem. Biotechnol. 40, 455-458 (1997)
  9. Liu, X.Q., Baek, W.S., Kyun, A.D., Choi, H.Y, and Yook, C.S., The Constituents of the Aerial Part of Gastrodia elata Blume. Nat. Prod. Sciences 8, 137-140 (2002)
  10. Noda, N., Kobayashi, Y., Miyahara, K., and Fukahori, S., 2,4-Bis (4-hydroxybenzyl) phenol from Gastrodia elata. Phytochemistry 39, 1247-1248 (1995) https://doi.org/10.1016/0031-9422(95)00051-8
  11. Shin, C.S., Park, C.K., Lee, J.W., Lee, J.G., Jang, C.K., and Kim, Y.K., Analysis of the Components with Freeze Drying and Steam Drying of Gastrodia elata Blume. J. Korean Soc. Food Sci. Nutr. 28, 1058-1063 (1999)
  12. Society of Oriental Medicine, The Modem Oriental Medicine, Hakchang-Sa, Seoul, Korea, 1993, pp. 446-447
  13. Taguchi, H., Yoshioka, I., Yamasaki, K., and Kim, I.H., Studies on the constituents of Gastrodia elata Blume. Chem. Pharm. Bull. 29, 55-62 (1981) https://doi.org/10.1248/cpb.29.55
  14. Wu, C.R., Hsieh, M.T., Huang, S.C., Peng, W.H., Chang, Y.S., and Chen, C.F., Effects of Gastrodia elata and its active compounds on scopolamine-induced amnesia in rats. Planta Med. 62, 317-321 (1996) https://doi.org/10.1055/s-2006-957892
  15. Zhou, J., Yang, Y.B., and Yang, T.R., The chemistry of Gastrodia elata Blume: The isolation and identification of chemical constituents of Gastrodia elata Blume (Chinese). Acta Chim. Sin. 37, 183-189 (1979)