• Journal of Applied Biological Chemistry
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• 제51권3호
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• pp.88-94
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• 2008

Three different protein extraction methods-phenol extraction, trichloroacetic acid (TCA) precipitation, and desalting/TCA precipitation-were compared to determine the optimal reproducible high resolution 2-dimensional (2-D) electrophoresis for each chungkugjang, doenjang, and kochujang samples. The soluble proteins from Chungkugjang extracted by phenol were separated with high reproducibility and resolution, and gained 1.75- to 3-fold more protein spots on 2-D gel than those from the other methods. On the contrary, the extracted proteins from doenjang and kochujang treated by desalting/TCA precipitation method showed about 1.5- to 3.3-fold more protein spots on 2-D gel. Using the established methods, the changes in the protein profiles of the fermented soy foods were monitored during the fermentation period by 2-DE. One of the major proteins in soy, $\beta$-conglycinin $\alpha$-subuint, and some proteins with unknown functions were localized on 2-D gel as the protease-resistant proteins throughout the fermentation period of doenjang. Changes in the protein profile monitored by the established methods can provide basic information on unfolding the mechanisms of the generation of biofunctional activity in the fermented soy foods.

• 한방안이비인후피부과학회지
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• 제28권2호
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• pp.85-92
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• 2015

Object : To report the case of the improved patient after the extraction with hemostst forcept. Methods : We treated one patient who visited Gabsan Korean medical clinic.First two times, We used acupuncture, fumigation, exsanguination, external medincine. He had some agonistic response. But It was not satisfactory. So We operated extraction with hemostat forceps. Result and Conclusion : 1. We extracted nasal polyp, the length of polyp was 5.5 cm2. After extraction, the patient had not headache, nasal congestion and so on.3. On intranasal endoscopy, left nose did not have polyp any more.4. Hemostat forceps extraction is effective to nasal polyp.

• 대한화학회지
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• 제38권3호
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• pp.246-253
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• 1994

미국 환경청이 지정한 129종의 priority pollutants 중 11종의 비휘발성 산성 유기화합물을 대상물질로 선정하여 토양시료 중의 극미량 분석방법을 연구하였다. 분석방법으로 기체크로토그래프/질량분석기- SIM(selected ion monitoring)법을 검출방법으로 정하고, 토양시료의 추출 및 농축방법으로 사용한 초음파 추출법(sonication extraction)과 Soxhlet(Soxhlet extraction)을 비교 연구하였다. 각 화합물의 회수율, 상대표준편차 및 method detection limit를 측정하여 EPA분석법을 대체할 수 있는지의 가능성을 조사하였다.

• Journal of International Society for Simulation Surgery
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• 제2권1호
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• pp.7-9
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• 2015

Purpose In the many face-related application such as head pose estimation, 3D face modeling, facial appearance manipulation, the robust and fast facial feature extraction is necessary. We present the facial feature extraction method based on shape regression and feature selection for real-time facial feature extraction. Materials and Methods The facial features are initialized by statistical shape model and then the shape of facial features are deformed iteratively according to the texture pattern which is selected on the feature pool. Results We obtain fast and robust facial feature extraction result with error less than 4% and processing time less than 12 ms. The alignment error is measured by average of ratio of pixel difference to inter-ocular distance. Conclusion The accuracy and processing time of the method is enough to apply facial feature based application and can be used on the face beautification or 3D face modeling.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.107-117
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• 2007

Objectives : This study is desinged in order to evaluate the Poison extraction method for the Atopic dermatitis. Methods : The authors observed the two cases of Atopic dermatitis patients who previously used steroid-based ointment. for treating the Poison Extraction Method. Conclusions : 1. In case 1, the patient with mild case of Atopic dermatitis in face is treated with the Poison extraction method. Rash symptoms intensed for first few days. As sweating appeared in the local area from the seventh day, all the symptoms was disappeared rapidly. No recurrence was found. 2. In case 2, the patient with severe case of Atopic dermatitis in whole body is treated with the Poison extraction method. The symptoms intensed after two months and all the symptoms of itchiness, rash, scaly letter dissapeared in the third and fourth months. No recurrence was found. 3. In both cases of mild and severe cases of Atopic dermatitis. all the symptoms were disappeared and no recurrence was found with the Poison Extraction Method.

• 한국연초학회지
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• 제23권1호
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• pp.65-70
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• 2001

In order to compare the extraction patterns of main components from the raw material between the extraction methods, the aerial parts (dried stem, leaves, and flowers) of Agastache rugosa were extracted by SDE simultaneous steam distillation & extraction) and SFE (supercritical fluid extraction). Volatile components of essential oil and extract were identified by GC and GC-MSD. The contents of essential oil extracted by SDE were 0.49% in aerial part of Agastache rugosa on dry basis. Major components were methyl chavicol(27.2%), isomenthone(24.6%), hexadecanoic acid(13.0%). menthone (5.5%) among 32 kinds of components confirmed in essential oil. On the other hand, the contents of SFE extracts revealed 3.21% on dry basis, 6 times higher than those of SDE. Major components were isomenthone(15.3%), hexadecanoic acid(13.7%), methyl chavicol(12.6%), benzoic acid(3.8%) among 33 kinds of components identified in extract.

• International Journal of Industrial Entomology and Biomaterials
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• 제26권2호
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• pp.113-118
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• 2013

In this study, silk sericin films were prepared using different extraction methods, and the molecular conformation of sericin was examined using Fourier-transform infrared spectroscopy with attenuated total reflection geometry. Additionally, the effect of the film side (air-facing side or plate-facing side) on the molecular conformation of the sericin films was investigated. Interestingly, the molecular conformation of the sericin film depended on the film side. The molecular conformation of air-facing side of the sericin film was significantly influenced by extraction solution and time. The ${\beta}$-sheet crystallization and the crystallinity index of the sericin film markedly increased with an increase in the extraction time in hot water. The order of the crystallinity indices for the sericin films obtained with different extraction solutions was as follows: citric acid solution > urea solution >> hot water. In contrast, no remarkable differences were observed in the molecular conformation of the plate-facing side of the sericin film after extraction in hot water for different time periods. Urea and citric acid solution extractions showed remarkably higher crystallinity indices for sericin than those obtained after hot water extraction. However, no significant differences were observed in the crystallinity index of sericin between urea and citric acid solution extraction in plate-facing side of the film.

• 펄프종이기술
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• 제48권1호
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• pp.93-99
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• 2016

The extraction efficiency depending on the extracting methods and the conditions of extraction was investigated. The common steam extraction was compared to the distillation extraction method. The effects of the samples size and the extraction time on the extract yield were also investigated by using UV-Vis spectrophotometer. One of the functional components of pine needle extract as the natural phenol base components were detected by the UV-VIS at around 235 nm wavelength range. The absorbance intensity at around 235 nm wavelength of the pine needle extract was used as the indicator of the extraction efficiency in this experiment. The distillation extraction showed the higher extract yield than the steam extraction. The grinding treatment of pine needles also resulted in the better extract performance, but the severe grinding showed a little decrease in the extract yield especially in case of the distillation extraction method. More than half of the extract was collected at the first stage of the extraction, that was the first 15 minutes in the total 60 minutes extraction.

• 정보과학회 논문지
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• 제42권3호
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• pp.367-378
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• 2015

본 논문은 기계학습 방법과 필터링 방법을 결합해서 경쟁관계를 인식하는 방법에 대한 연구이다. 기존 연구들은 기계학습 방법에만 의존해서 관계유형을 인식하는 연구들이 대부분이며. 사용되는 자질도 일반적인 관계유형에 적합한 자질을 사용하고 특히 구문분석 정보가 매우 중요한 자질로 사용된다. 본 논문에서는 구문분석 등의 언어분석 결과를 이용하지 않고, 단순한 자질들(어휘, 거리, 위치, 단서단어)만을 사용해도 경쟁관계 인식에 효과적임을 확인하였다. 또한, 경쟁관계인식 긍정 정확도를 향상시킬 수 있는 문장별 경쟁유무 분류방법, 스팸분류 방법, 거리제약 기반 자질필터링 방법을 기계학습 방법과 결합한 방법론을 제안한다. 방법론 검증을 위해서 뉴스분야 2,565개 문장을 평가셋으로 구축하였고, 비교 평가를 위해서 규칙기반 경쟁관계 인식기와 기존연구의 관계추출 방법론에 기반한 일반 관계추출기를 적용해서 비교하였다. 성능평가 결과로 규칙기반 엔진이 긍정정확도와 전체정확도(accuracy)가 81.2%와 56.8% 성능을 보였고, 일반 관계추출기는 61.2%와 56.3%를 보였다. 그에 비해서 본 논문에서 제안하는 방법은 긍정 정확도 92.2%와 전체정확도 71.3% 성능을 보여서 경쟁관계 인식에 효과적임을 확인하였다.

• 한국식품저장유통학회지
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• 제24권5호
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• pp.631-637
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• 2017

톳의 산업적 활용도 증진을 위하여 다양한 추출방법(열수, 고온가압, 초고압)을 이용하여 항산화 및 생리활성 특성을 평가하였다. 총 폴리페놀 및 플라보노이드 함량의 경우 전체적으로 가압가열 추출물에서 각각 30.51 mg/g, 4.78 mg/g으로 높은 함량을 나타내었다. 추출방법에 따른 톳 추출물의 항산화 활성을 평가하고자 DPPH와 ABTS 라디칼 소거활성 및 환원력을 실시하였으며 그 결과, 모든 실험구에서 농도 의존적으로 증가하는 경향을 보였으며 특히 고온가압 추출물이 전체적으로 높은 활성을 보였다. 그러나 지방산패 및 XO 억제활성의 경우 초고압 처리한 톳 추출물이 1 mg/mL의 농도에서 각각 61.93%, 33.68%로 가장 높은 활성을 나타내었다. 또한 tyrosinase 및 elastase 저해활성은 1 mg/mL의 농도에서 초고압 추출물이 각각 40.51%, 73.79%로 가장 높은 활성을 보였으며 고온가압, 열수의 순으로 나타났다. 톳의 경우 온도와 압력을 가했을 때 유용물질의 용출이 용이해져 다양한 항산화 및 생리활성을 나타내는 것을 알 수 있었으며 고온가압 및 초고압 추출물은 소재 활용가치가 높은 것으로 사료되며 기능성 증진을 위한 천연 식품소재로 활용 가능할 것으로 판단된다.