• Analytical Science and Technology
• /
• v.34 no.4
• /
• pp.153-159
• /
• 2021

In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 ㎍/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 ㎍/mL and 0.08-0.26 ㎍/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.

• Korean Journal of Optics and Photonics
• /
• v.14 no.4
• /
• pp.413-422
• /
• 2003

We have calculated the power extraction efficiency and the lasing wavelength distribution of index-coupled DFB lasers at threshold for various kL and facet reflectivity combinations, and compared with those above-threshold. The power extraction efficiency increases as the asymmetry of the facet reflectivities increases. The power extraction efficiency above-threshold is slightly larger than that at threshold. Since the relative photon density around the center region increases as kL increases, the power extraction efficiency decreases. The uniformity of the distribution of lasing wavelength over the stop band increases due to the relief of mode degeneracy as the asymmetry of the facet reflectivities increases. In the case of AR-HR combination, the lasing wavelength distributions at threshold are similar to those above-threshold. However, in the case of AR-AR combination, the lasing wavelength at threshold is concentrated on both edges of the stop band, while it is concentrated only on the longer wavelength edge above-threshold. As kL increases, the range of the lasing wavelength distribution increases due to the increase of the stop band. The effect of AR reflectivity on the power extraction and the lasing wavelength distribution is very weak.

• Natural Product Sciences
• /
• v.19 no.2
• /
• pp.166-172
• /
• 2013

Caffeine and epigallocatechin gallate (EGCG) are major constituents of green tea, the leaves of Camellia sinensis (Theaceae). Although EGCG is well known for diverse beneficial effect, caffeine is sometimes harmful with adverse effects. Therefore, the extraction efficiency was investigated using different extraction method such as extraction solvent, extraction time, extraction method, and repeated extraction. The content of EGCG and caffeine in green tea extract was quantitated by HPLC analysis. The extraction condition exerted difference on the extraction yield. The content of EGCG was also affected by different extraction condition. Especially, the extraction solvent greatly affected the content of EGCG in the extract. However, the content of caffeine was less affected compared to that of EGCG. The inhibitory effect of green tea extract on pancreatic lipase was almost similar regardless of extraction condition. Taken together, optimization of extraction condition will provide best efficacy for further development of green tea as anti-obesity therapeutics.

• Korean Chemical Engineering Research
• /
• v.52 no.1
• /
• pp.88-91
• /
• 2014

In this study, oil as a source of biodiesel from Nannochloropsis oceanica was extracted using organic solvent. The oil extraction yield and efficiency from dry and wet microalgae were investigated. The initial fatty acids content of the N. oceanica was 317.8 mg/g cell showing a high oil content over 30%. The yield from dry microalgae was higher than that from wet microalgae due to the inhibition of water. The yield by chloroform-methanol was the highest and the yield by hexane was the lowest. However, the total fatty acids contents with the chloroform-methanol were 678.7 and 778.2 mg/g oil under dry and wet conditions, respectively. The high oil extraction yield by chloroform-methanol reflected the fact that the extracted oil contained a high level of impurity. The hexane-methanol extraction from dry N. oceanica showed high oil extraction efficiency, 82.6%. The chloroform-methanol extraction under wet condition also showed high efficiency, 88.0%. While the hexane-methanol extraction from dry microalgae is desirable under low drying cost, the chloroform-methanol extraction from wet microalgae is desirable under high drying cost.

• Analytical Science and Technology
• /
• v.25 no.2
• /
• pp.146-151
• /
• 2012

An analytical method was developed for the measurement of uranium isotope in ground water using the liquid scintillation counting technique. A LKB Wallac Quantulus 1220 liquid scintillation counter (LSC) equipped with pulse shape analyzer (PSA) and a solvent extraction method were used for the measurement of uranium isotope in ground water samples. The effect of solution volume on the extraction efficiency was evaluated for 100 to 1000 mL solutions using a NIST standard reference material (NIST SRM 4321C). The effect of groundwater pH on the extraction efficiency was also investigated for pH ranging from 0.5 to 10. It was found that the extraction efficiency had a strong dependence on pH showing a maximum at pH 2. In contrast, the effect of groundwater volume on the extraction efficiency was negligible in the range investigated. According to the method, the extraction efficiency of uranium isotopes was near 96% and the lower detection limit for uranium was 0.018 Bq/L with the counting time of 300 min. The result of this study was also verified by the conventional ICP-MS measurement. It is demonstrated that the suggested method is valuable to the determination of the optimum extraction and measurement conditions for uranium in ground water. The method was successfully applied to the ground water at four locations near the Daejeon province. It was found that the uranium content and the isotopic ratio of $^{234}U/^{238}U$ at the locations ranged 0.59~6.69 Bq/L and 0.72~1.40, respectively.

• Fire Science and Engineering
• /
• v.20 no.4 s.64
• /
• pp.58-64
• /
• 2006

Smoke extraction in tunnel fire is investigated experimently with thermal model. The object is a immersed tunnel, of which the partial extraction system exists between the tubes. The model tunnel is measured 12 m long, 0.5 m wide and 0.35 m high. The fire is simulated to pool fire and the size corresponds to full scale fire of 5 MW based on Froude modeling. The performance of partial extraction system is determined under two ventilations, natural and longitudinal ones. The results show that compared with longitudinal ventilation, the smoke extraction efficiency of natural ventilation is increased about 30% because of smoke stratification in tunnel. Also the efficiency is identical to the iso-thermal model. The results will be help for activation of the ventilation system in emergency such as in the event of tunnel fires.

• Journal of the Optical Society of Korea
• /
• v.18 no.6
• /
• pp.772-776
• /
• 2014

We report a numerical analysis of the light-extraction efficiency (LEE) of light-emitting diodes (LEDs) on patterned sapphire substrates (PSSs). We considered various n-sided, regular convex pyramids, where n is an integer and $n{\geq}3$. We then considered four cross sections: extruded, subtracted, truncated-extruded, and truncated-subtracted. Ray-tracing simulations were carried out with these polygonal pyramid patterns, and the dimensions of the patterns were systematically varied. Optimized pattern shapes were determined for large LEE. An extruded circular pyramid with a slant angle of $45^{\circ}$ was found to be the optimal patterned shape.

• Journal of the Optical Society of Korea
• /
• v.18 no.4
• /
• pp.370-376
• /
• 2014

We optimized the design of the flat encapsulant of a light-emitting diode (LED) package to obtain higher light output power (LOP), both by experiment and simulation using three-dimensional ray-tracing software. In the experiment, the refractive index of the encapsulant was varied (1.41 and 1.53). In addition, double-layer structures with these refractive indices (1.41/1.53) were investigated by varying the shape of the interface between the two among flat, concave, and convex. The experiments showed that the LOP of the double-layer encapsulant with convex interface increased by 13.4% compared to the single-layer encapsulant with a refractive index 1.41, which was explained by the increase of the light extraction efficiency (LEE) in connection with the increase of the critical angle (${\theta}_c$) and the decrease of the Fresnel reflection.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2007.08a
• /
• pp.471-472
• /
• 2007

We have demonstrated that the light extraction efficiency of GaN based light emitting diodes (LEDs) can be significantly enhanced by using photonic crystal and microcavity (PCMC) effects. It was found that the extraction efficiency of the PCMC-LEDs is 9.5 times larger than that of the normal LEDs.

• Current Optics and Photonics
• /
• v.7 no.5
• /
• pp.496-503
• /
• 2023

We present a detailed analysis of the light extraction efficiency (LEE) of a core-shell nanorod light emitting diode (LED) using finite-difference time-domain (FDTD) simulations. We found that the LEE has a deep dependence on source positions and polarization directions based on the calculated LEE results for every x and z position inside the core-shell nanorod structure. The LEEs are different for the upper part (pyramid) and the lower part (sidewall) of the core-shell nanorod owing to total internal reflection (TIR) and the generated optical modes in the structure. As a result, the LEE of sidewall is much larger than that of pyramid. The averaged LEE of the core-shell nanorod LED is also investigated with variable p-GaN thickness, n-GaN thickness, and height for the design guidelines for the optimized LEE of core-shell nanorod LEDs.