• Journal of Life Science
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• v.25 no.11
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• pp.1204-1213
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• 2015

A comprehensive analysis of the population structure of the sandfish (Arctoscopus japonicas), the most abundant fishery resource in the East Sea of Korea, has not been carried out, despite its importance in Korea. The present study examined the genetic diversity and differences between five populations (two Japanese and three Korean populations) of A. japonicas captured in the East Sea using both the 401 bp sequence of mitochondrial DNA (mtDNA, cytochrome b) and five microsatellite DNA (msDNA) markers. The results of the analysis using the Cyt b sequence revealed 27 haplotypes. Based on msDNA variations, the estimated expected heterozygosity (H_E) in each population ranged from 0.68 (Gampo, Korea) to 0.7765 (Erimo, Japan). Pairwise F_ST and AMOVA tests using both the Cyt b sequence and msDNA data pointed to significant differences between the Korean and Japanese populations (mtDNA; F_ST=0.2648, p<0.05, msDNA; F_ST=0.0814, p<0.05). These results were similar to the results of UPGMA, PCA, and structure analysis. In these analyses, the five populations were assigned to two groups (Korean populations and Japanese populations). These results shed light on the genetic diversity and relationships of A. japonicas and contribute to research on the evaluation, conservation, and utilization of Korean A. japonicas as genetic resources.

• Korean Journal of Applied Biomechanics
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• v.16 no.3
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• pp.19-31
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• 2006

이 연구의 목적은 각 분절의 마커세트와 무릎관절 중심 정의가 3차원 무릎 관절각을 산출하는데 얼마나 민감하게 영향을 미치는지를 연구하였다. 자료수집은 1명을 실험대상자로 하여 두 가지 형태의 각기 다른 분절의 정의와 무릎관절의 중심을 나타내는 반사마커들을 동시에 오른쪽 하지에 부착시켜 실험을 실시하였다. 실험대상자의 달리기동작 중 좌측으로 45도 방향전환동작의 지지기를 분석하였다. 이를 위해서 8대의 고속카메라들을 이용하였고 달리기속도는 4m/$sec{\pm}(10%)$로 통제하였다. 하지분절의 발분절에는 하나의 마커세트를, 정강이와 대퇴분절에는 두 가지의 다른 마커세트들을 부착시켰다. 발분절에는 3개의 마커를 신발의 뒷부분에 부착하였고 정강이분절을 정의하기 위하여 첫 번째 마커세트는 경골을 중심으로 3개의 마커들을 두 번째 마커세트는 비골을 중심으로 3개의 마커를 부착하였다. 대퇴분절의 마커세트를 정의하기 위하여 첫 번째 마커세트에는 대퇴골을 중심으로 3개의 마커를 두 번째 마커세트에는 대퇴근육을 중심으로 3개의 마커들을 부착하였다. 무릎관절중심을 정의하는데 두 가지 다른 정의가 적용되었다. 첫 번째 무릎중심을 무릎의 내측과 외측의 마커들을 통해 두 마커의 중심을 무릎관절의 중심으로 정의하였다. 두 번째 무릎중심정의는 무릎의 외측부분과 슬개골의 중심에 부착된 마커들로부터의 교차점을 무릎관절중심으로 산출하였다. 무릎관절의 각도를 산출하기 위해서 JCS(Joint Coordinate System)의 정의가 적용되었고 연구의 결과는 다음과 같았다. 두 가지의 다른 분절마커세트 사이에서 무릎의 신전(extension)과 굴곡(flexion)은 유사한 형태를 나타냈으며 최대 무릎굴곡(peak knee flexion)각에서 $4.746^{\circ}$의 차이를 나타냈다. 다른 분절마커세트 사이의 회전(rotation)각과 내전(adduction)/외전(abduction)에서는 서로 다른 형태를 나타내었고, 두 마커세트간 최대무릎외측회전(peak knee external rotation)각도에서는 $15.628^{\circ}$의 차이를 나타냈다. 또한, 각 분절마커세트 내에서 두 가지의 다른 무릎관절 중심의 정의가 얼마나 무릎도 산출에 영향을 미치는지를 비교했을 때 무릎의 최대외측회전(peak external rotation)각에서 차이를 나타내었다. 첫 번째 분절마커세트의 무릎관절중심정의의 형태변화에 따라 최대외측회전각은 $0.549^{\circ}$의 차이를 나타냈고, 두 번째 분절마커 세트에서 무릎관절중심정의의 형태변화에 따라 최대외측회전각은 $0.309^{\circ}$의 차이를 나타냈다. 이와 같이 분절을 나타내는 마커세트와 무릎관절중심정의의 형태변화에 따라 무릎간을 계산하는데 있어서 결과가 다르게 산출되었다. 즉, 관절각의 계산이 분절에 부착되는 마커의 정의 혹은 위치에 매우 민감하게 영향을 받았다. 따라서 연구자가 여러 실험대상자들을 대상으로 실험시 마커세트 혹은 마커들을 동일한 위치에 가깝게 부착하는 것이 마커부착으로부터 발생하는 실험오차를 줄일 수 있을 것이다.

• Journal of Korean Neurosurgical Society
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• v.38 no.3
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• pp.202-205
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• 2005

Objective : The purpose of this study is to measure the dimensions of foramen ovale and to localize the zygomatic point using computed tomography[CT] in Korean adults with idiopathic trigeminal neuralgia. Methods : Facial axial CT scans using the orbitomeatal plane were performed in 67patients [39males and 28females; mean age 58.8years] with idiopathic classic trigeminal neuralgia. We measured the size of the foramen ovale and localized the zygomatic point which was a skin marker over the ipsilateral zygoma that approximates the lateral projection of a straight line joining the centers of the two foramen ovale. Results : The axial dimensions of the foramen ovale on the orbitomeatal plane were of average length : $8.18{\pm}0.82mm$ [range $6.9{\sim}11.5mm$]. width : $4.06{\pm}0.86mm$ [$2.5{\sim}5.7mm$]. The average distance between the external acoustic meatus and the zygomatic point was $21.64{\pm}1.99mm$ [$16.3{\sim}25.0mm$] and the average distance of anterior margin of condylar process of mandible to zygomatic point was $4.29{\pm}1.19mm$ [$1.0{\sim}7.0mm$]. Conclusion : The anatomical understandings including the size of the foramen ovale and localization of the zygomatic point could be helpful in determining a plan of percutaneous approaches to foramen ovale.

• Journal of Marine Life Science
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• v.5 no.1
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• pp.17-24
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• 2020

We have identified a heat shock protein 70 gene from freshwater snail, Semisulcospira coreana. The freshwater snail HSP70 gene encode a polypeptide of 639 amino acids. Based on bioinformatic sequence characterization, HSP70 gene possessed three classical signature motifs and other conserved residues essential for their functionality. The phylogenetic analysis showed that S. coreana HSP70 had closet relationship with that of golden apple snails, Pomacea canaliculata. The HSP70 mRNA level was significantly up-regulated in response to thermal and salinity challenges. These results are in agreement with the results of other species, indicating that S. coreana HSP70 used be a potential molecular marker in response to external stressors and the regulatory process related to the HSP70 transcriptional response can be highly conserved among species.

• Laboraroty Animal Research
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• v.34 no.4
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• pp.279-287
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• 2018

Placenta specific 8 (PLAC8, also known as ONZIN) is a multi-functional protein that is highly expressed in the intestine, lung, spleen, and innate immune cells, and is involved in various diseases, including cancers, obesity, and innate immune deficiency. Here, we generated a Plac8 knockout mouse using the CRISPR/Cas9 system. The Cas9 mRNA and two single guide RNAs targeting a region near the translation start codon at Plac8 exon 2 were microinjected into mouse zygotes. This successfully eliminated the conventional translation start site, as confirmed by Sanger sequencing and PCR genotyping analysis. Unlike the previous Plac8 deficient models displaying increased adipose tissue and body weights, our male Plac8 knockout mice showed rather lower body weight than sex-matched littermate controls, though the only difference between these two mouse models is genetic context. Differently from the previously constructed embryonic stem cell-derived Plac8 knockout mouse that contains a neomycin resistance cassette, this knockout mouse model is free from a negative selection marker or other external insertions, which will be useful in future studies aimed at elucidating the multi-functional and physiological roles of PLAC8 in various diseases, without interference from exogenous foreign DNA.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.05a
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• pp.228-231
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• 2022

Most CCTVs operated by public institutions for crime prevention and parking enforcement are located on roads. The angle of these CCTV's view is often changed for various reasons, such as bolt loosening by vibration or shocking by vehicles and workers, etc. In order to effectively provide AI services based on the collected images, the service target area(ROI, Region Of Interest) must be provided without interruption within the image. This is also related to the viewpoint of effective operation of computing power for image analysis. This study explains how to maximize the application of artificial intelligence technology by setting the ROI based on the marker on the road, setting the image analysis to be possible only within the area, and studying the process of finding the ROI.

• Computers and Concrete
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• v.33 no.5
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• pp.535-544
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• 2024

In the rapidly advancing landscape of computer vision (CV) technology, there is a burgeoning interest in its integration with the construction industry. Camera calibration is the process of deriving intrinsic and extrinsic parameters that affect when the coordinates of the 3D real world are projected onto the 2D plane, where the intrinsic parameters are internal factors of the camera, and extrinsic parameters are external factors such as the position and rotation of the camera. Camera pose estimation or extrinsic calibration, which estimates extrinsic parameters, is essential information for CV application at construction since it can be used for indoor navigation of construction robots and field monitoring by restoring depth information. Traditionally, camera pose estimation methods for cameras relied on target objects such as markers or patterns. However, these methods, which are marker- or pattern-based, are often time-consuming due to the requirement of installing a target object for estimation. As a solution to this challenge, this study introduces a novel framework that facilitates camera pose estimation using standardized materials found commonly in construction sites, such as concrete forms. The proposed framework obtains 3D real-world coordinates by referring to construction materials with certain specifications, extracts the 2D coordinates of the corresponding image plane through keypoint detection, and derives the camera's coordinate through the perspective-n-point (PnP) method which derives the extrinsic parameters by matching 3D and 2D coordinate pairs. This framework presents a substantial advancement as it streamlines the extrinsic calibration process, thereby potentially enhancing the efficiency of CV technology application and data collection at construction sites. This approach holds promise for expediting and optimizing various construction-related tasks by automating and simplifying the calibration procedure.

• The Korean Journal of Nuclear Medicine Technology
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• v.15 no.2
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• pp.116-124
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• 2011

Purpose: To establish accurate external quality assurance (EQA) test, cross institutional and modality tests were performed using WHO certificated reference material (CRM) and same pooled patients serum. Materials and Methods: Accuracy and precision were evaluated using CRM and pooled patients' serum for AFP, CEA, PSA, CA 125, CA 19-9, T3, T4, Tg, TSH. To evaluate the accuracy and precision, recover test and coefficient variation were measured. RIA test were performed in major 5 RIA laboratory and EIA (CLIA) test were done in 5 major EIA laboratory. same sample of CRM and pooled serum were delivered to each laboratory. Results: In 2009, mean precision of total tumor marker of RIA was $14.8{\pm}4.2%$ and that of EIA(CLIA) was $19.2{\pm}6.9%$. In 2010, mean precision of 5 tumor marker and T3, T4, Tg, TSH was $13.8{\pm}6.1%$ in RIA and $15.5{\pm}7.7%$ in EIA (CLIA). There was no significant difference between RIA and EIA. In RIA, the coefficient variations (CV) of AFP, CEA, PSA, CA 125, T3, T4, TSH were within 20%. The CV of CA 19-9 was over 20% but there was no significant difference with EIA (CLIA) (p=0.345). In recovery test using CRM, AFP, PSA, T4, TSH showed 92~103% of recovery in RIA. In recovery test using commercial material, CEA, CA 125, CA 19-9 showed relatively lower recovery than CRM but there was no significant difference between RIA and EIA (CLIA). Conclusion: By evaluating the precision and accuracy of each test, EQA test could more accurately measured the quality of each test and performance of laboratory.

• Progress in Medical Physics
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• v.21 no.3
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• pp.291-297
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• 2010

This study examined the dosimetric influence of implanted gold markers in proton therapy and the effects of their positions in the spread-out Bragg peak (SOBP) proton beam. The implanted cylindrical gold markers were 3 mm long and 1.2 mm in diameter. The dosimetric influence of the gold markers was determined with markers at various locations in a proton-beam field. Spatial dose distributions were measured using a three-dimensional moving water phantom and a stereotactic diode detector with an effective diameter of 0.5 mm. Also, a film dosimetry was performed using Gafchromic External Beam Treatment (EBT) film. The GEANT4 simulation toolkit was used for Monte-Carlo simulations to confirm the measurements and to construct the dose-volume histogram with implanting markers. Motion data were obtained from the portal images of 10 patients to investigate the effect of organ motions on the dosimetric influence of markers in the presence of a rectal balloon. The underdosed volume due to a single gold marker, in which the dose was less than 95% of a prescribed amount, was 0.15 cc. The underdosed volume due to the presence of a gold marker is much smaller than the target volume. However, the underdosed volume is inside the gross tumor volume and is not smeared out due to translational prostate motions. The positions of gold markers and the conditions of the proton-beam field give different impacts on the dose distribution of a target with implanted gold markers, and should be considered in all clinical proton-based therapies.

• Korean Journal of Poultry Science
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• v.42 no.1
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• pp.51-59
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• 2015

Chickens are exposed to the external and internal stressors such as low and high temperature, high stocking density, feed restriction and disease. There have been a few studies on gene expressions through the investigation of chickens under direct exposure to the stress of high stocking density. The objective of the present study was to determine the expressions of genes associated with stress, endoplasmic reticulum (ER)-stress, lipid and glucose metabolism in two strains of chickens, Korean Native Chicken (KNC) and White Leghorn (WL), raised in high stocking density. A total of 164 chickens aged 40 weeks were randomly allotted to a $540cm^2/bird$ stocking density (control), whereas the chickens in a high density group were assigned in a $311cm^2/bird$ stocking density with feeding ad libitum for 10 weeks. Total RNA was extracted from the live for qRT-PCR. The expression levels of hsp70 and $hsp90{\alpha}$ were higher in WL subjected to stress with high stocking density compared with those genes in control (P<0.05), while the expressions of genes were not affected in KNC. ER stress marker gene XBP1 was also highly expressed in WL with stress (P<0.05), but the stress of high stocking density did not influence to ER stress marker genes in KNC. Lipid metabolism associated genes including FABP4, FATP1 and ACSL1 were highly expressed in WL compared with KNC when subjected to high stocking density stress (P<0.05). The expression of glucose transport gene GLUT2 and GLUT8 were increased in chickens exposured to the stress of high stocking density (P<0.05). The data indicate that WL is more sensitive to the stress of high stocking density compared with KNC and the stress may influence the modulation of lipid and glucose metabolism in the liver of chickens.