• 한국지능시스템학회논문지
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• 제13권3호
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• pp.371-376
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• 2003

본 논문에서는 효과적인 정보를 표현하는 Independent Component Analysis(ICA)-factorial 표현방법을 이용하여 얼굴감정 인식을 수행한다. 얼굴감정인식은 두 단계인 특징추출 과정과 인식과정에 의해 이루어진다. 먼저 특징추출방법은 주성분 분석(Principal Component Analysis)을 이용하여 얼굴영상의 고차원 공간을 저차원 특징공간으로 변환한 후 ICA-factorial 표현방법을 통해 좀 더 효과적으로 특징벡터를 추출한다. 인식단계는 최소거리 분류방법인 유클리디안 거리에 근거한 K-Nearest Neighbor 알고리즘으로 얼굴감정을 인식한다. 6개의 기본감정(기쁨, 슬픔, 화남, 놀람, 공포, 혐오)에 대해 얼굴 감정 데이터베이스를 구축하고 실험해본 결과 기존의 방법보다 좋은 인식 성능을 얻었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제25권1호
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• pp.111-114
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• 2012

In previous studies we have shown that a sw17255 gene was expressed in hemocyte-specific tissues of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). It was verified that the sw17255 core promoter region contains elements that regulate the expression of this gene in hemocyte tissue; the selected promoter region spans nucleotides -1 to -2,112 upstream of the start codon. Each of the luciferase reporter gene expression vectors under the control of 4 different kinds of promoter candidates, (-2,112/-1), (-1,640/-1), (-1,169/-1) and (-579/-1), and the control reporter plasmid DNA, were introduced into B. mori larval coelom by direct injection using a syringe. The promoter candidate [E] (-579/-1) showed more than 1.67 fold transcriptional activity compared to control promoter activity. Higher productivity of an expressed gene in the transgenic silkworm by this promoter combination could be achieved in the near future. The foreign recombinant protein could be easily harvested from the blood of the transgenic silkworm.

• Preventive Nutrition and Food Science
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• 제3권1호
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• pp.98-104
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• 1998

As an efffort ot construct LAB (latice acid bacteria), capable of utilizing starch as fermentation substrate without the aid of externally supplied enzymes, plasmid vectors containing the amyL($\alpha$-amylase/pullulansase gene) from Clostridium thermophydrosulfuricum, and glucoamylase cDNA from Asperigillus shirousamii were constructed and introduced itno E. coli and L. lactis. For expression in procaryotes , 1.9kb glucoamylase cDNA encoding the mature form of enzyme was PCR amplified and translationaly fused to a PCR amplified 260 bp fragment containing the promotor and secretion signals of amyl in the same reading frame. The production of $\alpha$-amylase, Apu, and glucoamlase in E. coli and L. lactis was confirmed by enzyme assay and zymography . Enzymeswere detected in both cellpellets and supernatants, indicating theworking of scretion signals in heterologous hosts. The efficiencies of secretion were varibale depending on the gene and host. The highest $\alpha$- amylase acitivity observed was 1.1 units and most activiity was detected from thecell pellets. The degree of gene expression in both hosts and the effect on the growth of hosts were examined.

• 한국정보통신학회논문지
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• 제16권5호
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• pp.932-938
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• 2012

얼굴 표정은 상호간 의사소통에 있어 중요한 의미를 갖는 것으로, 인간이 사용하는 다양한 언어보다도 수많은 인간 내면의 감정을 표현할 수 있는 유일한 수단이다. 본 논문에서는 쉽고 자연스러운 얼굴 표정 생성을 위한 근육 모델 기반 3D 얼굴 표정 생성 시스템을 제안한다. 3D 얼굴 모델의 표정 생성을 위하여 Waters의 근육 모델을 기반으로 자연스러운 얼굴 표정 생성에 필요한 근육을 추가하여 사용하고, 표정 생성의 핵심적 요소인 눈썹, 눈, 코, 입, 볼 등의 특징요소들을 중심으로 얼굴 근육과 근육벡터를 이용하여 해부학적으로 서로 연결된 얼굴 근육 움직임의 그룹화를 통해 얼굴 표정 변화의 기본 단위인 AU를 단순화하고 재구성함으로써 쉽고 자연스러운 얼굴 표정을 생성할 수 있도록 하였다.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.275-281
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• 1995

From the plasmid pYA300 carring a CMCase of Rhizobium fredii USDA193 plasmid was subcloned into pBluescript II KS(+)/pBluescript II SK(+) vectors and designated pYA500 and pYA600, respectively. Escherchia coli cells transformed with pYA500 porduced the CMCase more than with pYA600. The orientation of the cloned fragment in pBluescript vector had the effect on gene expression in E. coli background. When the 1.7 kb CMCase gene fragment of R. fredii USDA193 was hybridized to EcoRI-digested total DNA from R. meliloti and R. fredii USDA 191 the unique bands hybridized respectively, indicating that some genetic diversity exists in the EcoRI restriction enzyme site for CMCase gene in Rhizobium strains. The optimum pH of enzyme activity was 7 and the optimum temperature of that was nearly 37$\circ$C. The cellulase-minus derivatives of pYA500 were constructed by Tn5 insertional mutation. Among 6000 transconjugants, two mutant plasmids (designated pYA500::Tn5a and pYA500::Tn5b) were detected from the cellulase- negative transconjugants. The product of CMCase gene was analyzed by one dimensional SDS- PAGE of the cell extracts. About 45 kDa protein was considered to be a product of CMCase gene.

• 한국양식학회지
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• 제16권1호
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• pp.51-58
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• 2003

The first Indian transgenic fish was generated in 1991 using borrowed constructs from foreign sources. To construct transformation vectors for the indigenous fishes, growth hormone genes of rohu (r-CH), Labeo rohita and catfish, Heteropneustes fossilis were isolated, cloned and sequenced; their fidelity was confirmed in prokaryotic and eukaryotic systems. A vector was constructed with grass carp b-actin promoter driving the expression of r-GH. Rohu eggs are large. fragile and swell 2~3 times. when fertilized. Hence they were amenable only for electroporated sperm-mediated gene transfer. Accordingly, the sperm electroporation technique was standardized to ensure 25% hatchling survival and 37% Presumptive transgenics without suffering any deformity. Southern analysis confirmed genomic integration in 15% of the tested individuals (Ti) belonging to family lines 2 and 3: another 25% of the Juveniles (Te) were also proved transgenic but with the transgene persisting extrachromosomally for longer than 1 to 2 years. perhaps due to the presence of replicon in the vector. Transgenics belonging to different family lines grew 6~8 times faster than the respective controls. Difference in growth trends of Ti and Te within a family line was not significant. In the Ti family 3 remarkable growth acceleration was sustained for a period longer than 36 weeks but in those of family 2, it gradually decreased. All transgenic fishes including the rohu converted the food at a significantly higher efficiency. Barring the transgenic mudloach, all the other transgenic fishes consumed food at significantly reduced rate.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2001년도 The 8th International Symposium
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• pp.49-58
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• 2001

We tried to introduce two forsythia genes related in lignan biosynthesis, dirigent protein and pinoresinol/lariciresinol (P/L) reductase, into potatoes for accumulation of lignans in transgenic potatoes. We made binary vectors overexpressing dirigent protein gene and P/L reductase gene driven by a CaMV35S promoter and transformed into potatoes via Agrobacterium mediated transformation. And in order to control the metabolic flux of lignan biosynthesis pathway, we tried to inhibit chalcone synthase genes of potatoes by antisense inhibition technique also. We tried to use PCR screening method for selection of transgenic plants of different vectors. We tried to determine and compare lignan contents from different transgenic potato lines.

• 한국컴퓨터정보학회논문지
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• 제13권2호
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• pp.11-17
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• 2008

수중에서 그물의 형상변화는 다양한 벡터에 의해 영향을 받게 된다. 그러나 그물의 각 입자마다 모든 벡터의 영향을 계산하는 것은 정확성과 사실성은 증대하지만, 방대한 계산량으로 처리 시간이 많이 소요된다. 기존의 시뮬레이션 방법들은 물리적 사실성을 희생하고 시각적인 사실성을 유지하는 범위에서 수중 가상현실을 시뮬레이션으로 구현하였다. 본 논문에서는 입자들의 병렬처리를 통하여 물리적, 시각적 사실성을 모두 만족하는 시뮬레이션을 제안한다. 병렬처리를 위해서는 OpenMP를 이용하였고, 사실적 그래픽 표현은 OpenGL을 사용하여 구현하였다. 본 논문에서 구현한 시뮬레이션은 게임 및 해양수산 분야에서 모델분석이나 전문가 시스템구축을 위한 기초자료로 활용될 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제41권1호
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• pp.50-55
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• 2014

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

• 한국지리정보학회지
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• 제22권1호
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• pp.19-27
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• 2019

본 연구는 비무장지대 및 군사분계선의 길이를 측정한 것이다. 이를 위해 정전협정문 첨부지도를 사용했다. 이 지도들은 9개의 도엽으로, 지도에 표시된 군사분계선을 추출하기 위해 도곽 좌표를 근거로 ArcGIS의 지오레퍼런싱 기능을 사용하여 스캔된 이미지 지도에 좌표를 부여했다. 이 지도들을 스크린 디지타이징하여 군사분계선을 추출하였다. 추출된 벡터를 원도에 중첩시켜 정확도를 확인하였다. 그리고 기존 연구에서 추출된 벡터와의 비교 분석을 통해 검증을 시도했다. 기존 연구에서 추출된 벡터는 군사분계선의 굴곡 부분에서 오차가 있었지만, 본 연구에서 추출된 벡터는 정전협정문 첨부지도의 군사분계선과 정확하게 일치했다. 그 길이를 측정한 결과는 239.42km(148.77mile)이다. 이는 현재 비무장지대와 관련된 연구나 보고서 그리고 대중 매체에서 표기하는 '155마일 휴전선' 또는 '248km 비무장지대'라는 표현의 부적합을 의미하는 것이다. 본 연구는 향후 비무장지대와 관련된 연구와 국가 또는 지방자치단체의 비무장지대와 관련된 정책 결정에 있어서 정확한 길이에 대한 정의를 제공할 수 있을 것으로 생각된다. 그러나 국토지리정보원 등 국가 기관에서의 본 연구에 대한 검증이 필요하다고 생각된다. 이러한 검증 절차를 거친 후 국가 차원에서의 비무장지대 및 군사분계선 길이에 대한 명확한 정의를 기대한다.