International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지
DOI QR코드

DOI QR Code

Hemocyte-specific Promoter for the Development of Transgenic Silkworm, Bombyx mori

  • Park, Seung-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Goo, Tae-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association) ;
  • Choi, Gwang-Ho (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Association)
  • Received : 2012.08.13
  • Accepted : 2012.09.05
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In previous studies we have shown that a sw17255 gene was expressed in hemocyte-specific tissues of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). It was verified that the sw17255 core promoter region contains elements that regulate the expression of this gene in hemocyte tissue; the selected promoter region spans nucleotides -1 to -2,112 upstream of the start codon. Each of the luciferase reporter gene expression vectors under the control of 4 different kinds of promoter candidates, (-2,112/-1), (-1,640/-1), (-1,169/-1) and (-579/-1), and the control reporter plasmid DNA, were introduced into B. mori larval coelom by direct injection using a syringe. The promoter candidate [E] (-579/-1) showed more than 1.67 fold transcriptional activity compared to control promoter activity. Higher productivity of an expressed gene in the transgenic silkworm by this promoter combination could be achieved in the near future. The foreign recombinant protein could be easily harvested from the blood of the transgenic silkworm.

Keywords

References

  1. Abraham EG, Donnelly-Doman M, Fujioka H, Ghosh A, Moreira L, Jacobs-Lorena M (2005) Driving midgut-specific expression and secretion of a foreign protein in transgenic mosquitoes with AgAper1 regulatory elements. Insect Mol Biol 14, 271-279. https://doi.org/10.1111/j.1365-2583.2004.00557.x
  2. Chen XG, Marinotti O, Whitman L, Jasinskiene N, James AA, Romans P (2007) The Anopheles gambiae vitellogenin gene (VGT2) promoter directs persistent accumulation of a reporter gene product in transgenic Anopheles stephensi following multiple bloodmeals. Am J Trop Med Hyg 76, 1118-1124.
  3. Cong L, Cao G, Renyu X, Zhonghua P, Xiaojian Z, Zhou W, Gong C (2011) Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms. Biochem Bioph Res Co. 410, 721-725. https://doi.org/10.1016/j.bbrc.2011.05.157
  4. Garcia AC, Gitai DL, Humann FC, Paco-Larson ML, Monesi N (2011) Functional characterization of the sciarid BhC4-1 core promoter in transgenic Drosophila. BMC Mol Biol 12, 32. https://doi.org/10.1186/1471-2199-12-32
  5. Juven-Gershon T, Hsu JY, Theisen JW, Kadonaga JT (2008) The RNA polymerase II core promoter - the gateway to transcription. Current Opinion Cell Biology 20, 253-259. https://doi.org/10.1016/j.ceb.2008.03.003
  6. Juven-Gershon T, Kadonaga JT (2010) Regulation of gene expression via the core promoter and the basal transcriptional machinery. Dev Bio 339, 225-229. https://doi.org/10.1016/j.ydbio.2009.08.009
  7. Moreira LA, Edwards MJ, Adhami F, Jasinskiene N, James AA, Jacobs-Lorena M (2000) Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes. P Natl Acad Sci USA 97, 10895-10898. https://doi.org/10.1073/pnas.97.20.10895
  8. Nirmala X, Marinotti O, Sandoval JM, Phin S, Gakhar S, Jasinskiene N, James AA (2006) Functional characterization of the promoter of the vitellogenin gene, AsVg1, of the malaria vector, Anopheles stephensi. Insect Biochem Molec 36, 694-700. https://doi.org/10.1016/j.ibmb.2006.05.011
  9. Nolan T, Petris E, Muller HM, Cronin A, Catteruccia F, Crisanti A (2011) Analysis of two novel midgut-specific promoters driving transgene expression in Anopheles stephensi mosquitoes. PLoS One 6, e16471. https://doi.org/10.1371/journal.pone.0016471
  10. Park SW, Kang SW, Goo TW, Kim SR, Lee GG, Paik SY (2010) Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR. BMB Reports 43, 480-484. https://doi.org/10.5483/BMBRep.2010.43.7.480
  11. Park SW, Goo TW, Kim SR, Kang SW (2011) Analysis of Hemocyte-specific Gene Expression from Bombyx mori. Int J Indust Entomol 23, 137-142. https://doi.org/10.7852/ijie.2011.23.1.137
  12. Smale ST, Kadonaga JT (2003) The RNA polymerase II core promoter. Annu Review of Biochemistry 72, 449-479. https://doi.org/10.1146/annurev.biochem.72.121801.161520
  13. Xia Q, Cheng D, Duan J, Wang G, Cheng T, Zha X, Liu C, Zhao P, Dai F, Zhang Z, He N, Zhang L, Xiang Z (2007) Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori. Genome Biology 8, R162. https://doi.org/10.1186/gb-2007-8-8-r162
  14. Yoshida S, Watanabe H (2006) Robust salivary gland-specific transgene expression in Anopheles stephensi mosquito. Insect Mol Biol 15, 403-410. https://doi.org/10.1111/j.1365-2583.2006.00645.x

Cited by

  1. Transgenic characterization of two silkworm tissue-specific promoters in the haemocyte plasmatocyte cells 2018, https://doi.org/10.1111/imb.12360