• Animal cells and systems
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• 제2권1호
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• pp.81-86
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• 1998

Recent identification and characterization of plant homeobox genes suggest that they play important roles in morphogenetic events. OSH1, one of the rice homeobox genes, is thought to be related to organ development since the changes of OSH1 gene expression cause morphological abnormalities of leaves by the ectopic expression and is expressed during early embryogenesis. In this experiment, the expression pattern of OSH1 was analyzedinmutants by in situ hybridization, and OSH1's potential as a molecular marker was explored. Region-specific expression of OSH1 during early embryogenesis shows that OSH1 could be used as a molecular marker for characterizing embryo mutants. Although several organless and shootless mutants showed normal expression of OSM1, some mutants exhibited abnormal expression patterns. In a minute organless cle1-1 embryo whose epidermis resembled morphologically the epithelium of scutellum, OSH1 expression was limited to a small basal region. This expression pattern suggests the gross deletion of the basal part. In a radicleless mutant, odm115, OSH1 expression was detected in a basal region instead of subcentral region of the ventral side. Together with other characteristics (short embryo and normal adventitious roots), odm115 was estimated to be derived from the deletion of basal region. Among five shootless mutants, three showed normal expression of OSH1. In the shl2 embryo, no expression of OSH1 was observed. In the shl1 embryo, however, OSH1 expression was extended to a dorsal side, indicating that SHL2 might be related to dorsoventral patterning. The above results of in situ hybrydization clearly indicate that OSH1 can be utilized as a marker for characterizing gene functions of embryo mutants.

• 복식
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• 제24권
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• pp.157-176
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• 1995

The purpose of this treatise is to study external form and internal meaning of minimal expression fashion which appeared as a major stream in modern fashion trends to understand one aspect of modernism in fashion and also to gain insight into internal value of human beings through fashion. The results can be summarized as follows : Firstly , minimalism is a trend in art attempting to seek essence of the object by presenting simple and disciplined expressions by minimal formative means and minimal production process. Secondly, minimal expression in fashion means seeking simplicity an dpurity by using minimum design elements and minimal productive process. Thirdly, external from of minimal expression fashion can be created through application of following minimal expression techniques. 1. Minimal expression techniques in terms of line mean smooth curve flowing along body contours, straight lines of diagonal lines into desciplined silhouette or rendering internal contour lines. 2. Minimum expression techniques in terms of forms mean forms of smooth curves flowing along boyd or forms with simple geometric forms from qualitative aspects, In terms of volume it means quest for essence of pure body itself by revealing body as it is by minimizing the size of dress or its area and herein is contained using simple geometric pattern or utilizing textiles without any patterns.3. Minimal expression techniques by colors mean simple colors such as primary colors, colors without clear distinctions or natural colors and in terms of quantity it means quest for one color within one item of dress or combination of each items when getting dressed. 4. Minimal expression techniques in terms of fabrics mean fabrics with simple surfaced. In terms of quantity it means quest for essence of tight fitting thin textiles to human body or using transparent materials to human body thus exposing body contours as it is. 5. Minimal expression techniques in terms of productive process mean minimizing process of tailoring , sewing or ornamenting and seeking for simplicity and purity. 6. Minimal expression techniques in terms of manufacturing process mean selection of technique conveying simple image with disciplined simple image. Fourthly, minimal expression fashion with external expression as mentioned in the above lay body-priority style and its internal meaning can be asummed as quest for essence and purity of human body.

• Molecular & Cellular Toxicology
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• 제1권4호
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• pp.248-255
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• 2005

To understand the response of cancer cells to anticancer drugs at the gene expression level, we examined the gene expression changes in response to five anticancer drugs, 5-fluorouracil, cytarabine, cisplatin, paclitaxel, and cytochalasin D in NCI-H460 human lung cancer cells. Of the five drugs, 5-fluorouracil had the most distinctive gene expression signature. By clustering genes whose expression changed significantly, we identified three clusters with unique gene expression patterns. The first cluster reflected the up-regulation of gene expression by cisplatin, and included genes involved in cell death and DNA repair. The second cluster pointed to a general reduction of gene expression by most of the anticancer drugs tested. A number of genes in this cluster are involved in signal transduction that is important for communication between cells and reception of extracellular signals. The last cluster represented reduced gene expression in response to 5-fluorouracil, the genes involved being implicated in DNA metabolism, the cell cycle, and RNA processing. Since the gene expression signature of 5-fluorouracil was unique, we investigated it in more detail. Significance analysis of microarray data (SAM) identified 808 genes whose expression was significantly altered by 5-fluorouracil. Among the up-regulated genes, those affecting apoptosis were the most noteworthy. The down-regulated genes were mainly associated with transcription-and translation-related processes which are known targets of 5-fluorouracil. These results suggest that the gene expression signature of an anticancer drug is closely related to its physiological action and the response of caner cells.

• 한국산학기술학회논문지
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• 제13권3호
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• pp.1170-1177
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• 2012

본 연구는 유아 어머니의 양육스트레스, 분노, 분노표현 유형 정도를 파악하고 분노표현 유형에 영향을 미치는 변인을 알아보기 위한 서술적 조사연구이다. K시의 유치원과 어린이 집에 재원하고 있는 3-6세 아동의 어머니 205명을 대상으로 설문지를 배부하여 자료 수집을 실시하였다. 자료 분석은 SPSS 19.0 통계 program을 이용하여 실수, 백분율, 평균, 표준편차, t-test, ANOVA, 상관관계, 다중회귀분석을 실시하였다. 본 연구결과 양육스트레스의 평균 점수는 2.32점이었고, 상태분노 1.36점, 특성분노 1.85점으로 나타났다. 분노표현 유형의 하위척도인 분노표출의 평균 점수는 1.84점이었고, 분노억제 1.75점, 분노조절 2.31점으로 나타났다. 양육스트레스는 상태분노, 특성분노, 분노표출, 분노억제와 정적상관관계를 보였고 분노조절은 부적상관관계를 보였다. 상태분노는 특성분노, 분노표출, 분노억제와 정적상관관계, 분노조절은 부적상관관계를 보였다. 특성분노는 분노표출, 분노억제와 정적상관관계, 분노조절과 부적상관관계를 보였다. 분노표출은 분노억제와 정적상관관계이고 분노조절과 부적상관관계를 보였다. 분노표현 유형에 영향을 미치는 요인을 확인하기 위해 회귀분석을 실시한 결과 양육스트레스, 상태분노, 특성분노가 분노표현 유형에 유의하게 영향을 미치는 것으로 나타났다. 따라서 자녀양육에 필요한 정보와 기술 제공뿐 만 아니라 어머니의 분노를 조절하고 양육스트레스를 감소시킬 수 있는 부모교육프로그램의 개발이 필요하다.

• 한방안이비인후피부과학회지
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• 제26권1호
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• pp.19-34
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• 2013

Objective : This study was performed to evaluate the effect of immune reaction inductive substances such as PMA, LPS, DPE, DNCB and WP, the whitman pigra extracting substance at simultaneously on the translocation of $NF{\kappa}B$ towards to the nucleus and the mRNA expression patterns of various cytokine genes in THP-1 cells, monocytes of human. Methods : To analyze the cytokine genes expressions, the RT-PCR method was used, and measuring TNF-${\alpha}$ that had been secreted during cell culture by the ELISA method. The morphological changes were observed during THP-1 cell by a scanning electron microscope and the quantitative distribution of $NF{\kappa}B$ in the cell that was analyzed through immunocytochemistry and a confocal microscopy. Results : WP showed different influences onto the mRNA expression patterns of cytokine genes with PMA, LPS. DPE and DNCB according to the types of immune inductive substances in the THP-1 cells. Upon treating PMA and DPE on the THP-1 cells at the same time or either additionally treating WP thereon, the movement of $NF{\kappa}B$ increase towards the nucleus from cell cytoplasm was able to be observed. The expressions of IL-$1{\alpha}$ and IFN-${\gamma}$ induced by PMA and PMA+DNCB were suppressed by WP while the expression of TGF-${\beta}$ was promoted. Regarding the secretion pattern of TNF-${\alpha}$ according to the treatment of PMA, its secretion amount was incredibly increased by concurrent treatment of WP, however, in case of co-treatment of WP with PMA and DNCB, it was found that the secretion amount of TNF-${\alpha}$ decreased. Conclusions : In this study, the WP extracting substance was confirmed that it had an influence on expression patterns of cytokine genes according to the actions of a variety kinds of immune reaction inductive substances treated on the THP-1 cells. Especially, WP co-treatment with PMA and DNCB was suppressed the expression of inflammatory cytokines, such as IL-$1{\alpha}$, IFN-${\gamma}$ and TNF-${\alpha}$.

• Reproductive and Developmental Biology
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• 제30권4호
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• pp.271-277
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• 2006

Transforming growth $factor-{\beta}\;(TGF-{\beta})$ has been shown to have a positive effect on in vitro fertilization (IVF) and has been reported to stimulate meiosis at follicular level in variety of species. The study was designed to determine the expression patterns of $TGF-{\beta}1,\;TGF-{\beta}$ receptors type I, II and Smads gene in bovine oocytes and embryos. $TGF-{\beta}1$ and their receptors were observed in the unfertilized oocytes. $TGF-{\beta}1$ and type II receptor were not expressed at the blastocyst stage, however, only type I receptor was exclusively observed at the same stage. The blastocyst stage, in particular, showed high levels of mRNA expression patterns containing a $TGF-{\beta}1$ type I receptor. The mRNA expression pattern of Smad 2 at all stages of embryonic development was similar in all respect with $TGF-{\beta}1$ type I receptor. On the contrary, Smad 3 and 4 were expressed with high and low level mRNA at the blastocyst stage. In conclusion. it is suggested that $TGF-{\beta}1$ signaling may be regarded as an important entity during the preimplantation embryo development.

• 한국콘텐츠학회논문지
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• 제8권12호
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• pp.190-197
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• 2008

기계적 명령어인 정경 (Threading)과, 트레들링(Treadlings)을 토대로 디자인해야 하는 직조 디자인은 표현의 한계성이 강한 것이 특징이다. 때문에 연구되어진 많은 한국 전통문양 자료들 중에서도 특히 떡살에 의해 한계 지어지는 떡살문양의 표현이 서로의 접목을 용이하게 하여 떡살문양을 선택하여 디자인의 컨셉을 잡았다. 특히, 문자문양과 기하학문양은 문양의 일반적 특징인 아름다움의 추구보다는 자연에 대한 인간의 두려움과 경이로움을 토대로 하여 생겨난 종교적 갈망과 기원을 담고 있어 이러한 이미지들을 토대로 트윌(Twill)기법, 특히 그 안에서도 형태적 표현이 용이한 샐리낼슨(Sally Nielsen)의 로즈펫(Rosepath)의 정경을 중심으로 활용하여 한국적 문양을 직조구조로 디자인하였다. 이 연구를 통해 세계의 위버(weaver)들에게 우리의 문양을 소개하는 계기가 되고 우리나라 산업에서는 이러한 직물디자인이 산업체에서 활발하게 활용되어 고부가가치의 자산을 획득하고 우리의 우수한 문화를 상품화 하고 문화산업을 발전시키는데 도움이 되기를 희망한다.

• 한국실내디자인학회논문집
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• 제23권1호
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• pp.88-95
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• 2014

This study intended arousal of other viewpoints that deal with and understand spaces and shapes, by describing the concept of 'dimensions' into visual patterns. Above all, the core concept of spatial dimensions was defined as 'expandability'. Then, first, the 'golden ratio', 'Fibonacci sequence', and 'fractal theory' were defined as elements of each dimension by stage. Second, a 'unit cell' of one dimension as 'minimum unit particles' was set. Next, Fibonacci sequence was set as an extended concept into two dimensions. Expansion into three dimensions was applied to the concept of 'self-similarity repetition' of 'Fractal'. In 'fractal dimension', the concept of 'regularity of irregularity' was set as a core attribute. Plus, Platonic solids were applied as a background concept of the setting of the 'unit cell' from the viewpoint of 'minimum unit particles'. Third, while 'characteristic patterns' which are shown in the courses of 'expansion' of each dimension were embodied for the visual expression forms of dimensions, expansion forms of dimensions are based on the premise of volume, directional nature, and concept of axes. Expressed shapes of each dimension are shown into visually diverse patterns and unexpected formative aspects, along with the expression of relative blank spaces originated from dualism. On the basis of these results, the 'unit cell' that is set as a concept of theoretical factor can be defined as a minimum factor of a basic algorism caused by other purpose. In here, by applying diverse pattern types, the fact that meaning spaces, shapes, and dimensions can be extracted was suggested.

• Genomics & Informatics
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• 제4권1호
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• pp.16-22
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• 2006

The KFDA (Korea Food & Drug Administration) has performed a collaborative toxicogenomics project since 2003. Its aim is to construct a toxicology database of 12 compounds administered to mice at initial phase. We chose 6-MP (6-mercaptopurine) which has been used in the treatment of childhood leukemia. It was administered at low (0.224 mg/kg) and at high (2.24 mg/kg) dose (5 mice per group) intraperitonealy to the postnatal 6 weeks mice, then the serum and liver were collected at the indicated time (6, 24 and 72 h) after scarification. Serum biochemical markers for liver toxicity were measured and histopathologic studies also were carried out. The gene expression profiling was carried out by using Applied Biosystems 1700 Full Genome Expression Mouse. By self-organization maps (SOM), we identified groups with unique gene expression patterns, some of them are supposed to be related to 6-MP induced toxicity, including lipid metabolism abnormality, inflammatory response, oxidative stress, ATP depletion and cell death. The potential toxic effects appearing as gene expression changes are dependent of the time of 6-MP but independent of the dosage of it. This study would contribute to establishment of international database as well as national one about hepatotoxicity.

• 복식문화연구
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• 제19권6호
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• pp.1177-1192
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• 2011

As a free creature that can fly in the sky, the bird has been an object of admiration and mystery and a mediator between human beings and the heavens. This study aims to clarify the aesthetic value of birds through study of the various expressions of the bird image on clothing and by the formative will of designers. Based on the general examination of the bird image through the study of documents, this study is composed of a case analysis, which reveals on modern fashion designs. Additionally, the forms of expression of the bird image are organized according to the stereoscopic expression of shaping, the relief expression of handicraft decoration, the flat expression of surface design, and the characteristic expression of wings and feathers. The aesthetic characteristics revealed by the expression patterns are metaphorical symbolism, surrealistic humor, lyrical naturalness, and fantastical femininity. Since antiquity, the bird image continuously recreated by artists has offered aesthetic value in terms of formativeness in the external form and symbolism in the internal form. This study would help to understand the diversity of creation in contemporary fashion design.