• Title/Summary/Keyword: Expression patterns

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A study of osteonectin expression patterns in BAPN-induced cleft palate formed rats (BAPN으로 유도된 구개파열 백서의 osteonectin발현 양상에 대한 연구)

  • Tae, Ki-Chul;Kim, En-Chel;Lee, Sun-Kyeong
    • The korean journal of orthodontics
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    • v.30 no.4 s.81
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    • pp.433-440
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    • 2000
  • The purpose of this study was to investigate osteonectin expression patterns in cleft palate compare to normal palate rats. We used 4 pregnant rats, and beta-aminoproprionitrile was oral dose to rat according to 1g/kg body weight at gestation days 13 to induce cleft palate. Total 6 fetus was got with cleft formed, then 3 fetus was used for immunohistostain and 3 fetus was used for western blot analysis. Expression patterns of osteonectin in mesenchymal cells of cleft palate was more dilute to mesenchymal cells of normal palate with immnunohistostain, and width and length of band of maxilla in cleft palate was more thin than maxilla of normal palate with western blot study.

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Expression Patterns of Odorant-Binding Proteins (OBPs) in a Termite (Reticulitermes speratus) (일본흰개미 (Reticulitermes speratus)의 Odorant-Binding Proteins (OBPs) 발현 양상)

  • Ra, Jong-Bum;Kim, Jae-Kyung;Kim, Gyu-Hyeok
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.1 s.129
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    • pp.1-6
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    • 2005
  • This research was performed to investigate the expression patterns of odorant-binding proteins (OBPs) migrating hydrophobic semiochemicals such as pheromone to the olfactory receptors in a termite (Reticulitermes speratus). Antennas and legs were cut from soldier and worker termites, respectively, and RT-PCR were conducted to investigate the existence of the OBPs reported up to now. Blast search suggested that the OBPs obtained were highly homologues of the OBPs reported. In worker termites, OBP-1 was expressed in both antennas and legs, OBP-2 and OBP-3 were observed only in antennas. And in soldier termites, OBP-1 was shown in both antennas and legs, OBP-2 were not observed, and OBP-3 was found in both antennas and legs. The differences of expression patterns of OBPs between worker and soldier termites may be explained by their specialized peculiarity.

Emphasizing Emotional Expression through Vibration Patterns in Online Environments (진동패턴을 이용한 감성정보 표현의 극대화)

  • Jung, Chan-Hee;Paek, Jung-Min;Hahn, Min-Soo
    • 한국HCI학회:학술대회논문집
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    • 2007.02a
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    • pp.411-416
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    • 2007
  • 본 연구는 메신저 환경에서 상대방에게 감정을 표현할 때, 기존의 시청각적 감성정보 전달방식 이외에 촉각적 수단을 통하여 감성정보를 표현하는 진동기반 촉각 인터페이스를 제안한다. 이를 위해, 국내의 한 메신저 프로그램이 제공하고 있는 감성정보 기반의 이모티콘들을 분류하여 진동패턴을 기반으로 한 택타일 아이콘(tactile icons)으로 재정의한다. 그리고 사용자 실험을 통해 이의 실효성과 향후 응용 가능성을 검증한다.

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A Study on the Textile Design by Computer Graphics (컴퓨터 그래픽에 의한 텍 스타일 디자인 연구(II)

  • 남후선
    • Archives of design research
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    • v.4 no.1
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    • pp.51-59
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    • 1991
  • Computer Graphic design was developed for printed media and its way of expression has been progressed di\ulcornerversely. Especially, the practices of computer graphic are used in textile design enterprise. The use of computer graphics in courses of textile design was produced by various si$$\mu$ations of colors, size and shape in patterns. Then the completed textile was presented as photograph. This paper describes patterns of two dimensions, cloths of three dimensions in use of softwares-TIPS, LUMENCE, FREE STYLE andTOPAS. As mentioned above, we can design fashion with easy by using computer graphics.

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Effects of Aqueous Extracts of Pinus rigida on Protein and Isozyme patterns during Radish Germination (리기다소나무의 수용추출액이 무 종자의 발아과정에서 단백질과 동위효소 패턴에 미치는 영향)

  • 김용옥;이호준
    • The Korean Journal of Ecology
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    • v.21 no.6
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    • pp.771-777
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    • 1998
  • Aqueous extracts of Pinus rigida changed the electrophoretic patterns of total proteins and of hydrolytic enzymes such as peroxidase, esterase and amylase during the germination of radish (Raphanus sativus var. hortensis for. acanthiformis). When the extract treatment was finished, at the late stage of radish germination, aqueous extracts of P. rigida had suppressed the expression of 24 KD and 60 KD proteins. the extract induced new isozyme bands, indicating concomitant activity of peroxidases, esterase activities were stimulated in the cathodic region. The activity of amylase was enhanced by the extract.

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Batik characteristics of the Chinese minority Miao (중국 소수민족 묘족(苗族) 납염의 특성)

  • Long, Lulu;Lee, Younhee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.3
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    • pp.23-42
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    • 2021
  • The purpose of this study is to analyze the characteristics of the Chinese Miao people's Batik patterns and provide data in the field of fashion design for the utilization of the traditional patterns. This study investigated and analyzed the regional characteristics of Batik dyeing, which was listed on China's national intangible cultural heritage list; Danzhai, Anshun, Huangping, and Gonghyeon. The expressive characteristics were classified into four categories: symbolism, abstraction, decorativeness, and playfulness. The results are as follows. First, symbolism can be classified into the origins of the Miao, symbols of faith, history of migration, and the symbols of status with Batik patterns from different regions, seemingly involving various symbolic implications. Due to the absence of letters, Miao people expressed their emotions and history in the design patterns. A series of developments in recent years has led to incorporating the temperament of the Miao people, history of migration, expression of emotions, and ideal hopes in Batik dyed design patterns and the symbolic functions have been significantly emphasized. Second, the decorativeness is mainly characterized by repeated patterns of the Miao Batik dyeing design. The overall patterns demonstrate design features characterized by regularization and simplification, along with a sense of rhythm with the unique arrangement of patterns. Third, the abstractness of the Batik dyeing design patterns is often found in plant and animal patterns, properly delivering new designs created by Miao women by extracting and rearranging various elements, including dots, lines, and faces shown in natural images. Fourth, playfulness is expressed by the transformation and distortion of Miao Batik patterns, and were created by applying patterns or other elements to original forms to express animal or plant patterns in a playful way.

Various Expression Pattern of Beta-catenin in the Preimplantation Stage of Porcine Embryos

  • Han, Jee-Soo;Koo, Deog-Bon;Shin, Bo-Rami;Lee, Kyung-Kwang;Han, Yong-Mahn
    • Proceedings of the KSAR Conference
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    • 2003.06a
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    • pp.56-56
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    • 2003
  • Beta-catenin is very important in early development including involvement in cell adhesion, cell signaling, and developmental fate specification. Cell-cell interaction is an important process during mammalian embryonic development. In preimplantation embryos, embryonic compaction is the process of increased cellular flattening and adhesion of junctional complexes and results in a polarized distribution. Beta-catenin is associated with embryonic compaction in mammals. Here, we examined the relationship between beta-catenin expression and compaction in porcine embryos derived from in vitro fertilization. First of all, we investigated beta-catenin expression in each embryonic developmental stage and also focused on expression pattern according to full, partial and non-compaction at morula stage. We used the immunocyto-chemical method in this research. To confirm compaction affects on the embryonic development, we compared between compaction and developmental rates to the blastocyst. The result showed that compaction and non-compaction rates were 14.6% and 63.8% at 4 days after IVF, respectively The developmental rates to the blastocyst and their total cell number were 50.9% vs 36.4% and 41.4$\pm$11.5 vs 26.8$\pm$12.7 in compaction and non-compaction groups. Although no difference was detected in the ratio of ICM to total cells between two groups, total cell number of the blastocysts in compaction group was superior to that of the blastocysts in non-compaction group (P<0.05). Expression of beta-catenin appeared in the boundary of membrane surface between blastomeres in 2- and 4-cell stage, and observed irregular pattern from 8-cell to blastocyst stage. We also investigated beta-catenin expression pattern according to the degree of compaction in the 3 groups; full, partial (>50%) and non-compaction. The expression signal in fully compacted embryos was stronger than those of partial and non-compacted embryos. Especially, beta-catenin expression appeared various patterns in morula stage suggesting the aberrant distribution of beta-catenin is affected by compaction patterns. Our results suggest that abnormal beta-catenin expression was affected by embryo quality and further development in porcine embryos in vitro.

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Dihydropyrimidinase related protein-2 expression in focal ischemic rat brain and hypoxia-induced PC 12 cell

  • Chung, Myung-Ah;Kim, Hwa-Jung
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.199.1-199.1
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    • 2003
  • Ischemia-induced changes in protein expression may provide important insights into the mechanisms of cellular damage and their potential recovery. In the present study, to investigate protein patterns changed in ischemic condition, the cortical and striatal tissue samples from the permanent and transient ischemic rat brain obtained by middle cerebral occlusion were analysed by proteomic approchese using 20-PAGE and MALOI-MS. (omitted)

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Prolonged Gene Expression Following Erythrocyte-Mediated Delivery of TRAIL Plasmid DNA (혈구세포 수송체로 투여된 트레일 유전자의 혈중 발현 지속 효과)

  • Byun, Hyang-Min;Kwon, Kyoung-Ae;Shin, Jee-Young;Oh, Yu-Kyoung
    • Journal of Pharmaceutical Investigation
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    • v.33 no.4
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    • pp.261-265
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    • 2003
  • Tumor necrosis facto-related apoptosis-inducing ligand (TRAIL) is a recently identified member of the tumor necrosis factor cytokine superfamily. TRAIL has been shown to induce apoptosis in a number of tumor cells whereas cells from most of normal tissues are highly resistant to TRAIL-induced apoptosis. These observations have raised considerable interest in the use of TRAIL in tumor therapy. In this study we report the biodistribution fates and serum expression pattern of plasmid DNA encoding TRAIL (pTRAIL) delivered in erythrocyte ghosts (EG). pTRAIL was loaded into EG by electroportion in a hypotonic medium The mRNA expression of pTRAIL was prolonged following delivery in EG-encapsulated forms. EG containing pTRAIL showed significant levels of mRNA expression in the blood over 9 days. The organ expression patterns of pTRAIL delivered via EG, however, did not significantly differ from those of naked pTRAIL, indicating that the expression-enhancing effect of EG containing pTRAIL was localized to the blood. These results suggest that pTRAIL-loaded EG might be of potential use in the treatment of hematological diseases such as TRAIL-sensitive leukemia.

Analysis of C. elegans VIG-1 Expression

  • Shin, Kyoung-Hwa;Choi, Boram;Park, Yang-Seo;Cho, Nam Jeong
    • Molecules and Cells
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    • v.26 no.6
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    • pp.554-557
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    • 2008
  • Double-stranded RNA (dsRNA) induces gene silencing in a sequence-specific manner by a process known as RNA interference (RNAi). The RNA-induced silencing complex (RISC) is a multi-subunit ribonucleoprotein complex that plays a key role in RNAi. VIG (Vasa intronic gene) has been identified as a component of Drosophila RISC; however, the role VIG plays in regulating RNAi is poorly understood. Here, we examined the spatial and temporal expression patterns of VIG-1, the C. elegans ortholog of Drosophila VIG, using a vig-1::gfp fusion construct. This construct contains the 908-bp region immediately upstream of vig-1 gene translation initiation site. Analysis by confocal microscopy demonstrated GFP-VIG-1 expression in a number of tissues including the pharynx, body wall muscle, hypodermis, intestine, reproductive system, and nervous system at the larval and adult stages. Furthermore, western blot analysis showed that VIG-1 is present in each developmental stage examined. To investigate regulatory sequences for vig-1 gene expression, we generated constructs containing deletions in the upstream region. It was determined that the GFP expression pattern of a deletion construct (${\Delta}-908$ to -597) was generally similar to that of the non-deletion construct. In contrast, removal of a larger segment (${\Delta}-908$ to -191) resulted in the loss of GFP expression in most cell types. Collectively, these results indicate that the 406-bp upstream region (-596 to -191) contains essential regulatory sequences required for VIG-1 expression.