• 한국의류학회지
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• 제14권4호
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• pp.245-251
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• 1990

The purposes of the study were to efplain the independent influences of Asthetic Expression and Fashion on aesthetic evaluation in the clothing, to examine which in more important aesthetic components such as line/style, color, textile and detail in aesthetic evaluation in the clothing. Data were obtained from 221 female students living in Seoul area by eight photo-graphs of clothed bodies and a questionnaire. The data were analysied by Pearson's correlation, Analysis of valiables, scheffe-test and Regression analysis. The results of the study were as follows; 1. In the aesthetic evaluation of clothing, the $59.30\%$ of the total variance was explained by Aesthetic Expression, the $20.91\%$ of the total variance was explained by Fashion and $59.68\%$ of the total variance were explained by Aesthetic Expression and Fashion. More important variable of aesthetic evaluation was found to be an Aesthetic Expression. 2. Among aesthetic components such as line/style, color, textile and detail in aesthetic evaluation of the clothing, such general aspects as color and line/style were perceived at first and then specific ones like textile and detail.

• 대한수의학회지
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• 제45권1호
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• pp.7-15
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• 2005

To elucidate the roles of phospho-IkB expression in the development and progression of EAE, we investigated the expression of phospho-IkB in the central nervous system (CNS) of rats during experimental autoimmune encephalomyelitis (EAE) using immunohistochemistry and Western blot analysis. In Western blot analysis, the increased expression of phospho-IkB went parallel to severity of EAE. The expression of phospho-IkB increased significantly at the peak stage of EAE followed by gradual decrease. Immunohistochemical studies showed that the phospho-IkB immunoreactivity was mainly expressed in inflammatory cells (macrophages, T cells) and glial cells (astrocytes, microglial cells) at the peak stage of EAE and disappeared at the recovery stage. These findings suggest that the phosphorylation of IkB is closely associated with autoimmune inflammation in the CNS and plays an important role in the initiation and progression of EAE.

• Environmental Analysis Health and Toxicology
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• 제27권
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• pp.11.1-11.8
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• 2012

Objectives: Hepatotoxicity of acetaminophen has been widely studied. However, the adverse effects on the heart have not been sufficiently evaluated. This study was performed to investigate cytotoxicity and alterations of gene expression in cultured cardiomyocytes ($H_9C_2$ cells) after exposure to acetaminophen. Methods: $H_9C_2$ cells were incubated in a 10 mM concentration of acetaminophen for the designated times (6, 12, and 24 hours), and cytotoxicity was determined by the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Alteration of gene expression was observed by microarray analysis, and RT-PCR was performed for the three representative oxidative stress-related genes at 24 hours after treatment. Results: It revealed that acetaminophen was toxic to cardiomyocytes, and numerous critical genes were affected. Induced genes included those associated with oxidative stress, DNA damage, and apoptosis. Repressed genes included those associated with cell proliferation, myocardial contraction, and cell shape control. Conclusions: These findings provide the evidences of acetaminophen-induced cytotoxicity and changes in gene expression in cultured cardiomyocytes of $H_9C_2$ cells.

• 한국실내디자인학회논문집
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• 제21권5호
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• pp.135-144
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• 2012

Since Seoul was selected as the world design capital at the end of 2007, the endeavors to modernize the Korean unique beauty is becoming more tangible. Especially, the commercial spaces to be used by the general public and tourists have the value for study in the respect that such spaces for the national iconic images and have a large and far-reaching effect to transmit the national identity. Therefore, this study analyzed the design coordination properties in the Koreanity expression found in the modern commercial spaces. the foundation for the analysis was based on Leonard Koren's work on the arrrangement rhetoric theory, 'Arranging things(2003), and the contents of Korean expression were reviewed with professional jounals. the results are as follows: First, the design coordination properties in the Koreanity expression shown in modern commercial spaces are black-white contrast, natural materials, curved shapes, brevity, and simple beauty. Especially, some elements such as wood, traditional Korean papers, stones, mid-to-low chroma natural colors, checked patterns, and crazy patterns are used with overlapping and it is viewed that it is necessay to more aggressively seek for the disappeared traditional elements in the Korean modeling properties. Second, the concrete images of Korean expressions could be summarized as four adjective image groups, Natural, Subtitle, Gentle and Magnificent.

• Molecules and Cells
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• 제45권9호
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• pp.631-639
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• 2022

Breast cancer is the leading cause of cancer-related death in women worldwide, despite medical and technological advancements. The RhoBTB family consists of three isoforms: RhoBTB1, RhoBTB2, and RhoBTB3. RhoBTB1 and RhoBTB2 have been proposed as tumor suppressors in breast cancer. However, the roles of RhoBTB3 proteins are unknown in breast cancer. Bioinformatics analysis, including Oncomine, cBioportal, was used to evaluate the potential functions and prognostic values of RhoBTB3 and Col1a1 in breast cancer. qRT-PCR analysis and immunoblotting assay were performed to investigate relevant expression. Functional experiments including proliferation assay, invasion assay, and flow cytometry assay were conducted to determine the role of RhoBTB3 and Col1a1 in breast cancer cells. RhoBTB3 mRNA levels were significantly up-regulated in breast cancer tissues as compared to in adjacent normal tissues. Moreover, RhoBTB3 expression was found to be associated with Col1a1 expression. Decreasing RhoBTB3 expression may lead to decreases in the proliferative and invasive properties of breast cancer cells. Further, Col1a1 knockdown in breast cancer cells limited the proliferative and invasive ability of cancer cells. Knockdown of RhoBTB3 may exert inhibit the proliferation, migration, and metastasis of breast cancer cells by repressing the expression of Col1a1, providing a novel therapeutic strategy for treating breast cancer.

• Molecular & Cellular Toxicology
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• 제3권4호
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• pp.231-237
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• 2007

Exposure to DNA-damaging agents can elicit a variety of stress-related responses that may alter the expression of genes associated with numerous biological pathways. We used 19 k whole human genome chip to detect gene expression profiles and potential signature genes in human normal hepatocytes (THLE-3) by treatment of five direct acting mutagens, furylfuramide (AF-2), N-nitroso-N-methylurea (MNU), methylmethanesulfonate (MMS), 4-nitroquinoline-N-oxide (4-NQO) and 2-nitrofluorene (2NF) of the $IC_{20}$ concentration for 3 h. Fifty one up-regulated common genes and 45 down-regulated common genes above 1.5-fold by five direct-acting mutagens were identified by clustering analysis. Many of these changed genes have some association with apoptosis, control of cell cycle, regulation of transcription and signal transduction. Genes related to these functions, as TP73L, E2F5, MST016, SOX5, MAFB, LIF, SII3, TFIIS, EMR1, CYTL1, CX3CR1 and RHOH are up-regulated. Down-regulated genes are ALOX15B, xs155, IFITM1, BATF, VAV2, CD79A, DCDC2, TNFSF8 and KOX8. We suggest that gene expression profiling on mutagens by toxicogenomic analysis affords promising opportunities to reveal potential new mechanistic markers of genotoxicity.

• BMB Reports
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• 제42권10호
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• pp.661-666
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• 2009

Porcine pancreas development is not well studied at the molecular level despite being a therapeutic resource for diabetic patients. In this study, we investigated expression of lineage markers and performed proteomic analysis. Expression of the early lineage markers Pdx1 and Ptf1a was developmentally conserved between mice and pigs, whereas expression of the islet differentiation marker Pax4 was delayed in porcine compared with murine pancreas development. Proteomic analysis found that expression levels of chymotrypsinogen were down-regulated during porcine pancreas development while those of digestive enzymes like lipases, elastase and serine protease were up-regulated. In addition, specific isoforms of protein folding assistants such as protein disulfide isomerase and prefoldin were expressed at specific stages during the maturation of digestive enzymes. Taken together, these results show that development of the porcine pancreas is regulated by a concerted interplay of pancreas lineage marker proteins and other specified proteins, resulting in a functional endocrine and exocrine organ.

• Nuclear Engineering and Technology
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• 제22권2호
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• pp.95-100
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• 1990

유체 유동상의 인자로 구성된 핵연료집합체에 걸리는 수력적 양력의 정확한 표현식은 핵연료의 건전성 설계 및 해석에 중요한 인자이다. 그러나 현재까지 이 양력에 대한 이론적인 해석이 제대로 이루어지고 있지 않아 이 분야에 혼란이 빚어지고 있다. 본 논문에서는 핵연료 집합체에 걸리는 수력적 양력에 대한 정확한 표현식을 이론적인 고찰을 통하여 유도하였으며 또한 양력에 관련된 제반 힘 요소들 즉, 압력강하, 부력, 전단응력, 집합체하중, 상호간의 관계를 검토하였다. 유도된 정확한 이론식을 이용하여 양력에 관한 간이식 오차의 특성을 분석한 결과 오차는 4가지 항으로 구성됨과 총 오차의 크기는 노심 유량의 변화 방향에 따라 달라짐을 알 수 있었다. 정량적인 분석을 COBRAIV-I를 이용하여 수행한 결과 총 오차의 크기는 약 1% 정도임이 밝혀졌다.

• 한국미생물·생명공학회지
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• 제29권1호
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• pp.62-66
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• 2001

This study was performed to investigate antigenic expression patterns in the course of HSV-1 infection. In SDS-PAGE analysis, HSV-1 antigens were detected, and among them, antigens in the size of 39, 47, 63, 86, 101, 105, 135, 159, and 181 kDa appear to be expressed in the most dominant forms. BALB/c mice were infected with HSV-1 for 29 days and antigenic expression from HSV-1 was investigated by Western blot analysis using anti-HSV-1 sera collected every two days from BALB/c mice infected with HSV-1. Most of HSV-1 antigens appeared sporadically as the infection progressed. However, antigens in the sizes of 63kDa and 135kDa were expressed from day 1 and 3, respectively, and existed continuously during the course of infection for 29 days, suggesting that they are the most dominant antigens inducing immune response durign HSV-1 infection, and they could be the target antigens for the development of vaccines. The isotype levels of IgA, IgGl, and IgM increased till the 17 th day infection and then started to decrease. During this course. IgGl was the most dominant isotype. In an indirect immunofluorescent assay, antibodies exhibited surface binding to the Vero cell infected with HSV-1, demonstrating that HSV-1 antigens are expressed on the surface of Vero cells.

• 대한한방부인과학회지
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• 제19권3호
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• pp.1-12
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• 2006

Purpose : This study was aimed to investigate the inhibitory effect of Leonurus sibiricus on the proliferation of human uterine leiomyoma cells and the expression of gene related the mechanism of cell apoptosis. Methods : We counted the number of death cells treated with indicated concentration of Leonurus sibiricus and investigated cell death rate by MTS assay. Furthermore, flow cytometry analysis and DNA fragmentation assay were used to dissect between necrosis and apoptosis and then we observed the differential gene expression by western blot analysis. Results : Leonurus sibiricus significantly inhibited the proliferation of uterine leiomyoma cell in a dose-dependent and time dependent manner. Fluorescence activated cell sorter (FACS) analysis indicated that Leonurus sibiricus induced G1 cell cycle arrest. Leonurus sibiricus enhanced the expression of p27 and p53 with cell cycle arrest. Conclusion : These findings suggest that Leonurus sibiricus is a candidate agent for the treatment of uterine leiomyoma. p27, $p53^{1}$ may play an important role in Leonurus sibiricus-induced cell cycle arrest and cell growth inhibition.