• 한국콘텐츠학회논문지
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• 제16권12호
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• pp.327-345
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• 2016

본 연구는 인간발달 과정을 신경과학, 심리학, 운동학습, 근육 생리학 등의 행동과학 자료와 생체역학, 인체운동 분석, 운동조절 기반의 인지 동작치료 프로그램을 정서 행동장애 아동들에게 실시하여 표정에 따른 얼굴 움직임이 감정과 정서변화에 따라 어떤 특성을 갖는지를 표정 움직임의 변화로 정량화 하고자 하였다. 본 연구에서는 영상측정 및 키네마틱 분석의 정서 행동장애 아동의 표정변화를 중재 프로그램 피드백 자료로 활용하였고, 표정 변화를 통해 인지동작 치료프로그램 효과를 알 수 있었다. 또한 정서 및 행동치료의 영상분석과 키네마틱 분석의 정량적 데이터를 통하여 인간발달에 대한 융 복합적 측정 및 분석법을 적용하여 발달장애의 조기발견과 치료과정에 따른 데이터를 축적하는 것도 기대할 수 있었다. 따라서 본 연구의 결과는 아동뿐만이 아니라 자기표현이 부족한 장애와 노인, 환자에게도 확대 적용할 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제29권9호
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• pp.1470-1477
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• 2019

${\beta}_2$-adrenergic receptor (${\beta}_2-AR$) was expressed efficiently using Bac-to-Bac Baculovirus Expression System in Sf9 cells as a bio-recognition element for multianalyte screening of ${\beta}$-agonist residues in pork. Sf9 cells were selected as the expression system, and codon optimization of wild-type nucleic acid sequence and time-dependent screening of expression conditions were then carried out for enhancing expression level and biological activity. Under optimum conditions of multiplicity of infection (MOI) = 5 and 48 h post transfection, the protein yield was up to 1.23 mg/ml. After purification by chromatographic techniques, the purified recombinant protein was applied to develop a direct competitive enzyme-linked receptor assay (ELRA) and the efficiency and reliability of the assay was determined. The IC50 values of clenbuterol, salbutamol, and ractopamine were 28.36, 50.70, and $59.57{\mu}g/l$, and clenbuterol showed 47.61% and 55.94% cross-reactivities with ractopamine and salbutamol, respectively. The limit of detection (LOD) was $3.2{\mu}g/l$ and the relevant recoveries in pork samples were in the range of 73.0-91.2%, 69.4-84.6%, and 63.7-80.2%, respectively. The results showed that it had better performance compared with other present nonradioactive receptorbased assays, indicating that the genetically modified ${\beta}_2-AR$ would have great application potential in detection of ${\beta}$-agonist residues.

• 복식문화연구
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• 제10권1호
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• pp.76-88
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• 2002

The aim of this study was to describe how the type of appeal and linguistic expression of men's wear advertisement in magazines by analyzing domestic men's and ladies' magazines published from 1990 to 1999. The periodical change in advertisement trend was also discussed. 1. Type of Linguistic expression The type of linguistic expression used in advertisements far men's wear could be classified into the following five categories : emotional expression, sensual expression, egoistic expression, informative expression, and brand name expression. The periodical trend in the use of each type of linguistic expression was as follows: the occupancy rate for the type of emotional expression was roughly 50 percent in the early 90's (1990-1993) and this percentage gradually decreased during the mid 90's (1994-1196) until the late 90's (1997-1990). Despite the decrease in occupancy rate, this type was the most commonly used. While the occupancy rate fur the type of informative expression gradually increased during the decade, occupancy growth fur the type of brand name expression was slight until its rapid expansion in the late 90's. The occupancy rate for the type of egoistic expression steadily increased during the early and mid 90's but decreased to its original percentage in the late 90's. As for the sensual expression, its occupancy was insignificant up to the mid 90's and then rapidly decreased in the late 90's. 2. Type of appeal The type of appeal used in men's wear advertisements could be classified into the four categories, sensitive appeal, sex appeal, image appeal, and quality appeal. The periodical trend in the use of each type of appeal was as follows: sensitive appeal was most commonly used during the decade ; it had an occupancy rate over 50 percent in the early 90's but the rate gradually decreased during the mid and late 90's. Sexual appeal were the least commonly used; though the occupancy rate increased during the early and mid 90's it dropped dramatically in the late 97's. The occupancy rate for image appeal gradually decreased until its growth in the late 90's. The occupancy rate fur quality appeal was insignificant in the early 90's. This percentage rapidly increased in the mid 90's but relapsed in the late 90's.

• 아동학회지
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• 제26권5호
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• pp.233-244
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• 2005

This study examined the effect of visual art appreciation integrated with expression(integrated art appreciation) upon young children's drawing expression. The pretest-posttest control group design was chosen for the study. Twenty kindergarten children assigned to the experimental group were exposed to a program of integrated appreciation activity for 10 sessions. Another group of 20 kindergarten children were assigned to the control group taught by the traditional art teaching methods. Analysis of Covariance was applied for data analysis. Children in the experimental group showed higher scores in such drawing expressions as line, color, shape, composition, and theme.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.186-189
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• 2003

Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a potent immunogen as well as major virulence factor. In order to express the recombinant urease in tobacco plants, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a plant expression vector. The recombinant vector was transformed to tobacco plants. The integration of the recombinant plasmids into tobacco chromosomal genome was verified by genomic PCR. Expression to mRNA was confirmed by Northern blot analysis, and expression to recombinant urease protein was observed by Western blot analysis. These results showed that the recombinant urease can be produced in tobacco plants and will be tested for immune response to use as an edible vaccine.

• 콘크리트학회논문집
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• 제16권5호
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• pp.709-717
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• 2004

The existing AASHTO Standard Specification have some inadequacies in expressing wheel load distribution of bridge which has specific shape of curved bridge instead of straight bridge. Thus, this research presented the finite element analysis and modelling technique of prestressed concrete girder bridge having curved slab and the expression of wheel load distribution was suggested as the ratio of bending moment utilizing the result of finite element analysis of prestressed concrete girder bridge having cowed slab. The considered parameter of girder distribution expression is the curvature of slab, span length, girder space, cross beam space and number of lanes. Though the suggested girder distribution expression is generally underestimated below AASHTO Standard Specification, once the curvature of slab increases, the suggested expression gets larger than AASHTO LRFD Standard Specification.

• 미생물학회지
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• 제31권2호
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• pp.136-140
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• 1993

A awamori 의 glucoamylase 유전자와 A. nidolans 의 trpC maker 유전자를 가진 A. nidulans 의 expression vector 를 만들었다. 이재조합 plasmid 를 트립토판 영양요구주의인 A. nidulans B17 에 형질전환시켰다. Southern blotting 으로 vector DNA 가 A. nidulans 의 chromosomal DNA 에 integration 된 것을 확인하였다. Northern blotting 의 결과, glucoamylase 유전자는 induction 조건에서 mRNA 를 합성하였다. glucoamylase 의 역가가 형질전환체에서 증가하였으며, nondenaturing polyacrylamide gel 에서 glucoamylase 의 활성이 나타났다.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.285-289
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• 1994

An insecticidal crystal protein gene, cryIA(c), from Bacillus thuringiensis HD-73 was integrated into the chromosome of a xanthan-producing bacterium, Xanthomonas campestris XP92. The cryIA(c) gene expression cassette was constructed that placed the gene between the trc promoter and rrnB transcriptional terminator. The $lacl^q$ gene was also included to prevent the expression of cryIA(c) gene in X campestris cells. Southem blot analysis confirmed the integration of the cryIA(c) gene expression cassette in chromosome of X campestris XP92 transconjugant. Expression of the insecticidal crystal protein was confirmed by Western blot analysis and bioassay against the larvae of Hyphantria cunea (Lepidoptera： Arctiidae) and Plutella xylostella (Lepidoptera：Plutellidae).

• BMB Reports
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• 제31권5호
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• pp.427-431
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• 1998

In the previous report (Choi et al., 1997), the angiogenin binding peptides identified from a phage-peptide library were analyzed by using the fusion proteins composed of the Escherichia coli maltose binding protein and its corresponding peptides. However, it was difficult to obtain a sufficient amount of the fusion proteins required for further analysis because of the low expression level. We now report a high level expression of the fusion protein and analysis of its anti-angiogenin activity. The use of strong T7 promoter and removal of signal sequence allowed about a 20-fold increase in the expression efficiency of the fusion protein. We were able to obtain about 10 mg of purified fusion protein from one liter of culture. The purified fusion protein showed angiogenin-specific affinity and inhibited the binding of biotinylated actin to human angiogenin at $IC_{50}$ of 0.6 mM. Its anti-angiogenin activity was also revealed by the chorioallantoic membrane assay.

• Genomics & Informatics
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• 제15권1호
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• pp.38-47
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• 2017

Research into new methods for identifying highly expressed genes in anonymous genome sequences has been going on for more than 15 years. We presented here an alternative approach based on modified score of relative codon usage bias to identify highly expressed genes in crenarchaeal genomes. The proposed algorithm relies exclusively on sequence features for identifying the highly expressed genes. In this study, a comparative analysis of predicted highly expressed genes in five crenarchaeal genomes was performed using the score of Modified Relative Codon Bias Strength (MRCBS) as a numerical estimator of gene expression level. We found a systematic strong correlation between Codon Adaptation Index and MRCBS. Additionally, MRCBS correlated well with other expression measures. Our study indicates that MRCBS can consistently capture the highly expressed genes.