• Journal of Life Science
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• v.22 no.10
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• pp.1415-1419
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• 2012

Thymosin ${\beta}4$ ($T{\beta}4$) has been reported to be overexpressed in CD133-positive colorectal cancer stem cells. We analyzed the relationship between $T{\beta}4$ and CD133-positive stem cells in normal stomach by examining the expression patterns of $T{\beta}4$ and CD133 in normal stomach tissues by immunohistochemical staining; co-localization of $T{\beta}4$ and CD133 was studied by immunofluorescence and confocal laser-scanning microscopy. Both $T{\beta}4$ and CD133 were expressed in stomach glands and showed similar expression patterns. Immunofluorescence staining of $T{\beta}4$ and CD133 showed that the expression of $T{\beta}4$ and CD133 was co-localized. In summary, both $T{\beta}4$ and CD133 were expressed in glands of normal stomachs and expression patterns were co-localized. These data suggest that $T{\beta}4$ expression is strongly related to CD133 expression.

• Journal of Animal Science and Technology
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• v.63 no.5
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• pp.977-983
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• 2021

Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (USP9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dynamics during gametogenesis. However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X.

• Journal of Yeungnam Medical Science
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• v.20 no.2
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• pp.129-141
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• 2003

Background: Leptin is a 16-KDa non-glycosylated peptide hormone synthesized almost exclusively by adipocytes. The well-known function of leptin is regulation of food intake and energy expenditure. Leptin also plays a regulatory role in immune and inflammatory process including cytokine production. The purpose of this study was to investigate the effect of leptin on the expression of several chemokine genes(RANTES, IL-8, MCP-1, IP-10, Mig, MIP-$1{\alpha}$, MIP-$1{\beta}$, and GRO-${\alpha}$) in THP-1 cells. Materials and Methods: Total RNA of THP-1 cells were prepared by Trizol method, and then stimulated with the leptin(250 ng/$m{\ell}$) or LPS(100 ng/$m{\ell}$). We examined the expression patterns of various chemokine mRNAs in THP-1 cell lines by RT-PCR and Northern blot. Results: Leptin did not induce the expression of chemokine mRNAs in THP-1 cells. The expression patterns of RANTES, IL-8, MCP-1, IP-10, and Mig mRNAs in THP-1 cells stimulated with leptin and LPS simultaneously was almost same to the patterns of LPS alone-induced chemokine mRNAs. RANTES mRNA expression was independent on the concentrations of leptin. Although leptin did not have strong effect on the expression of RANTES, IL-8, MCP-1, IP-10, Mig, MIP-$1{\alpha}$, MIP-$1{\beta}$, and GRO-${\alpha}$ mRNAs in THP-1 cells, leptin could induce the expression of long isoform of leptin receptor(OB-RL) mRNA, and its expression was elevated in simultaneous stimulation of leptin and LPS. Conclusion: These data suggest that leptin is able to induce OB-RL in THP-1 cells, however, leptin has little effect on the expression of pro-inflammatory chemokine genes.

• Molecules and Cells
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• v.37 no.3
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• pp.241-247
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• 2014

Sudden cardiac death (SCD), which is primarily caused by lethal heart disorders resulting in structural and arrhythmogenic abnormalities, is one of the prevalent modes of death in most developed countries. Myocardial ischemia, mainly due to coronary artery disease, is the most common type of heart disease leading to SCD. However, postmortem diagnosis of SCD is frequently complicated by obscure histological evidence. Here, we show that certain mRNA species, namely those encoding hemoglobin A1/2 and B (Hba1/2 and Hbb, respectively) as well as pyruvate dehydrogenase kinase 4 (Pdk4), exhibit distinct postmortem expression patterns in the left ventricular free wall of SCD subjects when compared with their expression patterns in the corresponding tissues from control subjects with non-cardiac causes of death. Hba1/2 and Hbb mRNA expression levels were higher in ischemic SCD cases with acute myocardial infarction or ischemic heart disease without recent infarction, and even in cardiac death subjects without apparent pathological signs of heart injuries, than control subjects. By contrast, Pdk4 mRNA was expressed at lower levels in SCD subjects. In conclusion, we found that altered myocardial Hba1/2, Hbb, and Pdk4 mRNA expression patterns can be employed as molecular signatures of fatal cardiac dysfunction to forensically implicate SCD as the primary cause of death.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.8
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• pp.1000-1006
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• 2010

Phosducin (PDC) is a photoreceptor cell-specific protein that is phosphorylated by cyclic nucleotide-dependent protein kinase. PDC and PDC-like proteins (PDCL, PDCL2, and PDCL3) are members of a conserved family of small thioredoxin-like proteins that modulate the ${\beta}$- and ${\gamma}$-subunits of G-proteins. In mammals, Pdc, Pdcl, and Pdcl3 genes show ubiquitous expression; however, Pdcl2 gene expression is limited to the testis and ovary. The aim of the present study was to examine the expression patterns of chicken Pdcl2 (cPdcl2) during testicular and ovarian development. Protein sequence comparisons performed using the CLUSTAL X program revealed that the amino acid sequences and potential phosphorylation sites of cPDCL2 and mammalian PDCL2 proteins were highly conserved. Quantitative real-time PCR analysis revealed that cPdcl2 was differentially expressed in the testis and ovary. Specifically, cPdcl2 expression was detected at low levels in the ovary at all time points. In the testis, cPdcl2 expression was detected at low levels until 5 weeks of age. At 8 weeks of age, however, cPdcl2 showed increased expression levels in the testis. Using in situ hybridization, we detected high levels of cPdcl2 expression in the testis, particularly in the spermatocytes and round spermatids. In summary, our data describe expression patterns of germ cell-specific Pdcl2 during testicular and ovarian development in chickens.

• Cross-Cultural Studies
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• v.37
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• pp.309-334
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• 2014

This study is based on the Korean-Chinese parallel corpus, utilizing the Korean connective morpheme '-myenseo' and contrasting with the Chinese expression. Korean learners often struggle with the use of Korean Connective Morpheme especially when there is a lexical gap between their mother language. '-myenseo' is of the most use Korean Connective Morpheme, it usually contrast to the Chinese coordinating conjunction. But according to the corpus, the contrastive Chinese expression to '-myenseo' is more than coordinating conjunction. So through this study, can help the Chinese Korean language learners learn easier while studying '-myenseo', because the variety Chinese expression are found from the parallel corpus that related to '-myenseo'. In this study, firstly discussed the semantic features and syntactic characteristics of '-myenseo'. The significant semantic features of '-myenseo' are 'simultaneous' and 'conflict'. So in this chapter the study use examples of usage to analyse the specific usage of '-myenseo'. And then this study analyse syntactic characteristics of '-myenseo' through the subject constraint, predicate constraints, temporal constraints, mood constraints, negatives constraints. then summarize them into a table. And the most important part of this study is Chapter 4. In this chapter, it contrasted the Korean connective morpheme '-myenseo' to the Chinese expression by analysing the Korean-Chinese parallel corpus. As a result of the analysis, the frequency of the Chinese expression that contrasted to '-myenseo' is summarized into