• Title/Summary/Keyword: Experimental studies

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Studies on the Energy and Protein Requirement Determination for Broiler Production -4. Effect of Iso-calorie and Different Protein Level on Broiler Production- (Broiler 생산(生産)에 있어서 Energy와 Protein 요구량(要求量) 결정(決定)에 관한 연구(硏究) (제4보(第4報)) -동일(同一)Energy수준(水準)에서의 Protein 수준이 Broiler 생산(生産)에 미치는 영향(影響)-)

  • Kim, Dae Jin;Kim, Young Kil;Ko, Yong Du
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.4
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    • pp.395-400
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    • 1983
  • The experiment was conducted to study the effect of protein level on weight gain, feed consumption and the efficiencies of feed, energy and protein when the broiler chick was fed the diet of different protein level with the iso-calorie content. The energy and protein level of the basal ration was 3200 kcal and 22.85% for starter period and 3265 Kcal and 18.58% for finisher period, respectively. In order to increase the protein level of the experimental diet, the basal diet was substituted by fish meal by the ratio of 3, 6, 9, and 12%. The male Abor Acre broiler chicks were employed for 8 weeks and the result obtained were as follows. 1. The weekly body weight gain was significantly greater for Diet C than for Diet A and E in the starter period and greater for Diet A than for Diet E in the finisher period (P<0.05). 2. In stater period the feed intake was not affected by the protein level but in finisher period the feed intake for Diet C, D, and E was significantly reduced compared with Diet A. 3. As for feed conversion ratio, Diet C of 1.43 was improved significantly compared with Diet A of 1.58(P<0.05). 4. As the protein level of diet increased, the energy efficiency ratio increased and however, the protein efficiency ratio decreased in starter and finisher period. In conclusion the best performance of broiler is thought to be realized when the protein and energy level was 23% and 3200 Kcal for starter period and 20% and 3250 Kcal for finisher period, respectively.

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Morphological Traits of Selected Chestnut Races and its Propagation Studies (밤나무 우량품종(優良品種)의 형태학적(形態學的) 특징(特徵)과 증식(增殖)에 관(關)한 연구(硏究))

  • Yim, Kyong-Bin;Kwon, Ki-Won
    • Journal of Korean Society of Forest Science
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    • v.22 no.1
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    • pp.49-62
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    • 1974
  • 1. In the comparison of survival ratio among three different methods(bark graft, veneer graft, root graft), bark graft showed the highest survival ratio and root graft was the lowest. It was shown to be significant at the level of 5% in the analysis of variance for the results. It was able to be supposed that one of the main causes for the results was the different amounts of ferric tannic acid which was formated owing to the reaction of grafting knife with tannic acid oozing from cutting faces of the tree. In juvenile tissue graft, the survival ratio of inverted radicle graft was a little higher than that of juvenile stem graft, but there was no significant difference between two methods in analysis of variance. 2. The most hairs of chestnut tree leaves were recognized as stellates on the most part of leaves except for venation. The number of rays in the stellates was ranged from 4 to 8 generally. It was shown to be highly significant differences at the level of 1% among the each race growing at the similar environmental condition in the length of ray and the distributed ratio of the stellates having different ray number. 3. Excepting for the basal width of serration there were no significant differences between $Imakita_1$, $Imakita_2$ as well as between $Teteuchi_1$, $Teteuchi_2$ at the each point of experimental items in this study. Such results made this study more useful. 4. Among the races that were growing in the similar environmental condition, there were highly significant differences at the level of 1% in the length and the width of serration. 5. The rolling of hair, the angle of serration from the leaf margin, the existence of lateral vein in the serration, the intrusion of main vein into the serration and the width of main vein were observed to be somewhat useful as the subsidiary methods for the identification of chestnut races.

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Studies on the Ecology of Field Mice in Mt. Chuisuh (취서산(鷲棲山) 들쥐류(類)의 생태(生態)에 관한 연구(硏究))

  • Nam, Jung Chil
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.707-716
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    • 2001
  • This study was conducted four ecological survey of field mice of Mt. Chuisuh in Yangsan City, Kyungnam from June, 2000 to March, 2001 for the understanding of field mice damage to forest. The results are obtained as follows. 1. The species of field mice collected were Apodemus agrarius, Apodemus peninsulae and Eothenomys regulus, and Crocidura suaveolens of insectvore. 2. The total collecting rate were 8.1% collected 61 individuals of field mice from 758 trap. 3. The rate among the 61 field mice collected were 45.9% in Apodemus agrarius, 29.5% in Eothenomys regulus, 19.7% in Apodemus peninsulae, and 4.9% in Crocidura suaveolens. 4. The constitution of weight of field mice was 16-51.9g in Apodemus agrarius, 12-25.9g in Apodemus peninsulae, 16-45.9g in Eothenomys regulus, and 12-15.9g in Crocidura suaveolens. 5. The sex ratio of field mice was 48.0% : 52.0% in Apodemus agrarius, 50.0% : 50.0% in Apodemus peninsulae, 55.6% : 44.4% in Eothenomys regulus, and 66.7% : 33.3% in Crocidura suaveolens. 6. The average body lengh was 104.33mm in Eothenomys regulus, 96.04mm in Apodemus agrarius, 94.50mm in Apodemus peninsulae, and 76.33mm in Crocidura suaveolens. 7. The average tail lengh was 92.75mm in Apodemus peninsulae, 80.65mm in Apodemus agrarius, 41.0mm in Crocidura suaveolens, and 40.33mm in Eothenomys regulus. 8. The breeding period of field mice in Mt. Chuisuh presumes from about the early of March to end of October. Specially, The Eothenomys regulus was ascertained the breeding period in December, discovered first time in korea through this experimental study. 9. The average litter size of field mice was 4.5 in Apodemus agrarius, 4.7 in Apodemus peninsulae, and 3.4 in Eothenomys regulus.

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Antioxidative and Antimicrobial Activities of Monascus pilosus(Corn Silage Mold) Mycelial Extract and Its Culture Filtrate (Monascus pilosus 균사체 및 배양여액의 항산화 및 항균활성)

  • Kim, Jae-Won;Lee, Sang-Il;Kim, Sung-Hwan;Lee, Ye-Kyung;Kim, Soon-Dong
    • Food Science and Preservation
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    • v.17 no.5
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    • pp.741-751
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    • 2010
  • We evaluated the nutritional value of a Monascus pilosus mycelial ethanolic extract (MEM) and culture filtrate (CFM) by determining the contents of monacolin K and citrinin, and by measuring antioxidant and antimicrobial activities. The yields of freeze-dried MEM and CFM powder were 4.02% and 3.35% of wet weight, respectively. Pigment content ($OD_{500}$ value) of MEM (0.79) and CFM (0.63) were lower than those of commercial rice beni-koji ethanolic extracts (EERB) (0.87), but were in good agreement with the L*, a*, and b* values and the hue angles of the products. The total monacolin K content of MEM (24.91 mg%) was higher than those of CFM (1.27 mg%) and EERB (14.65 mg%). However, the active monacolin K content of EERB (5.48 mg%) was higher than those of MEM (3.35 mg%) and CFM (0.4 mg%). Citrinin was not detected in any sample. The total polyphenol content of MEM (4.68%, w/w) was similar to that of CFM (4.29%, w/w), thus 13.75.20.94% higher than that of EERB. The total flavonoid content of EERB was 6.8.7.0-fold higher than those of MEM (0.64%, w/w) and CFM (0.66%, w/w). The total antioxidant capacity of CFM (3.51%, w/w) was 1.62.2.08-fold higher than those of MEM (2.74%, w/w) and EERB (1.69%, w/w). The electron-donating capacities of 1% (w/v) solutions of CFM, MEM, BHT, and EERB were 86.20%, 77.25%, 77.25%, and 44.82%, respectively, and the corresponding reducing powers ($OD_{700}$ values) were 2.1, 2.4, 1.1, and 1.6, respectively. SOD(superoxide dismutase)-like activities were in the order MEM (39.85%) > BHT (37.68%) > EERB (26.70%) > CFM (21.5%). Although the TBARS (% value) of MEM was a little lower than that of BHT, it was higher than those of CFM and EERB. The antibacterial activities of CFM acting on Bacillus brevis and Escherichia coli were somewhat higher than those of MEM, whereas the activities of MEM on Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, and Salmonella enteritidis were higher than those of CFM. However, the antibacterial activities of MEM and CFM were less than those of EERB and BHT. In conclusion, although further studies are needed, we offer experimental evidence that the by-products of M. pilosus MEM and CFM contain significant antioxidant and antimicrobial activities that may be useful in the development of healthy foods.

Optimization of Antimicrobial Activity Against Food-borne Pathogens in Grapefruit Seed Extract and a Lactic Acid Mixture (식품위해미생물에 대한 자몽종자 추출물과 젖산 혼합물의 항균효과 최적화)

  • Kim, Hae-Seop;Park, Jeong-Wook;Park, In-Bae;Lee, Young-Jae;Kim, Jeong-Mok;Jo, Yeong-Cheol
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.472-481
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    • 2009
  • Response surface methodology (RSM) is frequently used for optimization studies. In the present work, RSM was used to determine the antimicrobial activitiesof grapefruit seed extract (GFSE) and a lactic acid mixture (LA) against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella typhimurium, Pseudomonas fluorescens, and Vibrio parahaemolyticus. A central composite design was used to investigate the effects of independent variables on dependent parameters. One set of antimicrobial preparations included mixtures of 1% (w/w) GFSE and 10% (w/w) LA, in which the relative proportions of component antimicrobials varied between 0 and 100%. In further experiments, the relative proportions were between 20% and 100%. Antimicrobial effects against various microorganisms were mathematically encoded for analysis. The codes are given in parentheses after the bacterial names, and were S. aureus ($Y_1$), B. cereus ($Y_2$), E. coli ($Y_3$), S. typhimurium ($Y_4$), P. fluorescens ($Y_5$), and V. parahaemolyticus ($Y_6$). The optimum antimicrobial activity of the 1% (w/w) GFSE:10% (w/w) LA mixture against each microorganism was obtained by superimposing contour plots ofantimicrobial activities on measures of response obtained under various conditions. The optimum rangesfor maximum antimicrobial activity of a mixture with a ratio of 1:10 (by weight) GFSE and LA were 35.73:64.27 and 56.58:43.42 (v/v), and the optimum mixture ratio was 51.70-100%. Under the tested conditions (a ratio of 1% [w/w] GFSE to 10% [w/w] LA of 40:60, and a concentration of 1% [w/w] GFSE and 10% [w/w] LA, 70% of the highest value tested), and within optimum antimicrobial activity ranges, the antimicrobial activities of the 1% (w/w) GFSE:10% (w/w) LA mixture against S. aureus ($Y_1$), B. cereus ($Y_2$), E. coli ($Y_3$), S. typhimurium ($Y_4$), P. fluorescens ($Y_5$), and V. parahaemolyticus ($Y_6$) were 24.55, 25.22, 20.20, 22.49, 23.89, and 28.04 mm, respectively. The predicted values at optimum conditions were similar to experimental values.

Effect of Lead Content on Atomic Structures of Pb-bearing Sodium Silicate Glasses: A View from 29Si NMR Spectroscopy (납 함량에 따른 비정질 Pb-Na 규산염의 원자 구조에 대한 고상 핵자기 공명 분광분석 연구)

  • Lee, Seoyoung;Lee, Sung Keun
    • Korean Journal of Mineralogy and Petrology
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    • v.34 no.3
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    • pp.157-167
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    • 2021
  • Lead (Pb) is one of the key trace elements, exhibiting a peculiar partitioning behavior into silicate melts in contact with minerals. Partitioning behaviors of Pb between silicate mineral and melt have been known to depend on melt composition and thus, the atomic structures of corresponding silicate liquids. Despite the importance, detailed structural studies of Pb-bearing silicate melts are still lacking due to experimental difficulties. Here, we explored the effect of lead content on the atomic structures, particularly the evolution of silicate networks in Pb-bearing sodium metasilicate ([(PbO)x(Na2O)1-x]·SiO2) glasses as a model system for trace metal bearing natural silicate melts, using 29Si solid-state nuclear magnetic resonance (NMR) spectroscopy. As the PbO content increases, the 29Si peak widths increase, and the maximum peak positions shift from -76.2, -77.8, -80.3, -81.5, -84.6, to -87.7 ppm with increasing PbO contents of 0, 0.25, 0.5, 0.67, 0.86, and 1, respectively. The 29Si MAS NMR spectra for the glasses were simulated with Gaussian functions for Qn species (SiO4 tetrahedra with n BOs) for providing quantitative resolution. The simulation results reveal the evolution of each Qn species with varying PbO content. Na-endmember Na2SiO3 glass consists of predominant Q2 species together with equal proportions of Q1 and Q3. As Pb replaces Na, the fraction of Q2 species tends to decrease, while those for Q1 and Q3 species increase indicating an increase in disproportionation among Qn species. Simulation results on the 29Si NMR spectrum showed increases in structural disorder and chemical disorder as evidenced by an increase in disproportionation factor with an increase in average cation field strengths of the network modifying cations. Changes in the topological and configurational disorder of the model silicate melt by Pb imply an intrinsic origin of macroscopic properties such as element partitioning behavior.

Study on the Expression of Matrix Metalloproteinase-1 by Promoter Polymorphism in Human Dermal Fibroblast (섬유아세포에서 프로모터 다형성에 의한 Matrix Metalloproteinase-1의 발현에 관한 연구)

  • Lee, Jin Woo;Jung, Yujung;Bong, Sim-Kyu;Park, No-June;Lee, Sang Heon;Noh, Minsoo;Lim, Kyung-Min;Kim, Su-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.47 no.3
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    • pp.205-212
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    • 2021
  • The skin fibroblasts of different origins showed different expression levels of MMP-1 in response to TNF-α treatment or UV irradiation. We hypothesized that this is caused by polymorphism in the MMP-1 promoter region. To elucidate it, first of all, we analyzed and classified the genotype of the -1607 site of the MMP-1 promoter in 23 commercially available primary fibroblasts, and then we examined the expression of MMP-1 by TNF-α or UVB stimulation for each classified genotype. As a result of the analysis, fibroblasts with 6 1G/1G genotypes, 10 1G/2G genotypes, and 7 2G/2G genotypes were identified. Hs68 and Detroit 551 cell lines were confirmed to have 1G/2G genotypes. In the 1G/1G genotype, MMP-1 was expressed twice as high as that of the control group by TNF-α treatment, and was hardly expressed by UV light. In the case of the 1G/2G genotype, MMP-1 was expressed 2.45 fold higher by TNF-α treatment, and 1.4 fold by UV light than the control. In the case of the 2G/2G genotype, MMP-1 was expressed 1.35 fold by TNF-α treatment, and was highly expressed by 2.5 fold by ultraviolet rays compared to control. It can be estimated that MMP-1 expression is better induced in the 1G genotype by TNF-α and in the 2G genotype by UV light. In addition, it can be presumed that MMP-1 expression is increased by creating a site where the Ets transcription factor can bind by another G inserted at the -1607 position. These studies have not been conducted at all in fibroblasts in relation to skin aging, so it is an area that needs to be further studied in the future. In conclusion, since the skin is an organ that is affected by both intrinsic aging and photoaging at the same time, when analyzing the expression of MMP-1 as a target for improving skin aging, it is necessary to select cells with a genotype suitable for the experimental conditions of the study.

Comparison of mDixon, T2 TSE, and T2 SPIR Images in Magnetic Resonance Imaging of Lumbar Sagittal Plane (요추 시상면 자기공명 영상검사에서 mDixon과 T2 TSE, T2 SPIR 영상의 비교 연구)

  • Jung, Da-Bin;Lee, Hae-Kag;Heo, Yeong-Cheol
    • Journal of the Korean Society of Radiology
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    • v.15 no.6
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    • pp.927-933
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    • 2021
  • The purpose of this study was to compare and analyze the differences in scan time, signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) in the third lumbar vertebral region including the back fat, spinal cord, and cerebrospinal fluid using the mDixon, T2 TSE, and T2 spectral pre-saturation with inversion-recovery (SPIR) techniques. With the factors affecting the SNR fixed, the lumbar sagittal plane images of 30 adults were compared on mDixon, T2 TSE, and T2 SPIR imaging tests. The test times for mDixon, T2 TSE, and T2 SPIR were 115 seconds, 60 seconds, and 60 seconds, respectively. The mDixon T2 images showed higher SNR than the T2 TSE images at the third lumbar vertebral region (p<0.05), lower SNR in the back fat and cerebrospinal fluid (p<0.05) areas, and comparable SNR in the spinal cord (p>0.05). The CNR between the third lumbar vertebral area and back fat was higher in the mDixon T2 images, and the CNR of the cerebrospinal fluid and spinal cord images was higher in the T2 TSE images (p<0.05). The mDixon T2 FS images CNR was lower for the 3rd lumbar vertebral body region and back fat than the T2 SPIR images, and higher for the spinal cord and cerebrospinal fluid images (p<0.05). The CNR between the third lumbar body and back fat areas was higher in the mDixon T2 FS images (p<0.05), and there was no difference in the CNR in the images of the cerebrospinal fluid and the spinal cord (p>0.05). It is difficult to determine whether the mDixon technique is superior to the conventional T2 TSE and T2 SPIR techniques in terms of test time, SNR, and CNR. This study was confined to patients with simple lower back pain and was limited by controlled experimental conditions. Studies using clinically applied protocols are warranted in the future.

Emodin Studies on Anti-inflammatory and Skin Barrier Improvement Activities (Emodin의 항염 및 피부장벽개선 활성 연구)

  • Kim, Se-Gie;Choi, Jae Gurn;Jang, Young-Ah
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.6
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    • pp.1383-1392
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    • 2021
  • It has been reported that emodin, a major pharmacologically active ingredient of herbal medicines such as Polygonum cuspidatum, Polygonum multiflorum, Rheum palmatum, and Aloe vera, is effective in antioxidant, antibacterial, anti-inflammatory, anticancer, and liver protection. In this study, to investigate the potential of emodin to be used as a skin disease and functional material, the activity related to the improvement of inflammation and skin barrier function was confirmed. To observe the anti-inflammatory effect on HaCaT cells, which are human keratinocytes, cytokine inhibition was confirmed by ELISA kit and protein expression by western blot. In HaCaT cells activated with TNF-α (10 ng/mL)/IFN-γ (10 ng/mL), emodin was treated with each concentration (5, 10, 20, 40) µM. As a result, It was confirmed that the production amount of TNF-α, IL-1β and IL-6 decreased as the concentration of emodin increased. In the experimental results on the expression levels of inflammation-related proteins iNOS and COX-2, it was confirmed that 48% of iNOS and 29% of COX-2 were inhibited compared to control at a concentration of 20 µM of emodin. As an indicator of skin barrier function improvement, the mRNA expression level of filaggrin, involucrin, and loricirn and the production amount of filaggrin, involucrin, and loricirn were confirmed. and excellent results were obtained with an emodin concentration-dependent increase. In particular, filaggrin, which was produced twice as much as the control at a concentration of 20 µM, is a protein involved in the formation of NMF, a natural moisturizing factor, and is known to play an important role in moisturizing the stratum corneum. In conclusion, it was confirmed that emodin can be used as a material for improving inflammation and improving skin barrier function, which is part of the potential for use as a skin disease and functional material. It is believed that if additional research is performed in the future, the scope of its application can be further expanded.

Simulation and Post-representation: a study of Algorithmic Art (시뮬라시옹과 포스트-재현 - 알고리즘 아트를 중심으로)

  • Lee, Soojin
    • 기호학연구
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    • no.56
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    • pp.45-70
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    • 2018
  • Criticism of the postmodern philosophy of the system of representation, which has continued since the Renaissance, is based on a critique of the dichotomy that separates the subjects and objects and the environment from the human being. Interactivity, highlighted in a series of works emerging as postmodern trends in the 1960s, was transmitted to an interactive aspect of digital art in the late 1990s. The key feature of digital art is the possibility of infinite variations reflecting unpredictable changes based on public participation on the spot. In this process, the importance of computer programs is highlighted. Instead of using the existing program as it is, more and more artists are creating and programming their own algorithms or creating unique algorithms through collaborations with programmers. We live in an era of paradigm shift in which programming itself must be considered as a creative act. Simulation technology and VR technology draw attention as a technique to represent the meaning of reality. Simulation technology helps artists create experimental works. In fact, Baudrillard's concept of Simulation defines the other reality that has nothing to do with our reality, rather than a reality that is extremely representative of our reality. His book Simulacra and Simulation refers to the existence of a reality entirely different from the traditional concept of reality. His argument does not concern the problems of right and wrong. There is no metaphysical meaning. Applying the concept of simulation to algorithmic art, the artist models the complex attributes of reality in the digital system. And it aims to build and integrate internal laws that structure and activate the world (specific or individual), that is to say, simulate the world. If the images of the traditional order correspond to the reproduction of the real world, the synthesized images of algorithmic art and simulated space-time are the forms of art that facilitate the experience. The moment of seeing and listening to the work of Ian Cheng presented in this article is a moment of personal experience and the perception is made at that time. It is not a complete and closed process, but a continuous and changing process. It is this active and situational awareness that is required to the audience for the comprehension of post-representation's forms.