• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.335-340
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• 1997

Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.

• Journal of the Korean Geosynthetics Society
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• v.16 no.2
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• pp.139-148
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• 2017

Ground subsidence caused by damaged water pipe and sewer is recently increasing due to the aging of city and pipeline in many city. Although many recent studies have verified characteristics of ground subsidence due to wastewater pipe breakdown, research about characteristics of ground subsidence due to water pipe is insignificant. subsidence due to water pipe is insignificant. This study aims to identify the ground failure mechanism caused by water and sewer pipe breakdown. Accordingly, we conducted an indoor model experiment to verify characteristics of ground subsidence considering characteristics of ground and ground failure. The water pipe pressure and velocity head was considered to find out ground subsidence mechanism. Also comparative analysis is conducted by analyzing relative density and fine-grain content considering embedded condition of water pipe. When the relative density and seepage pressure is low, small scale ground subsidence can occur, but when the conditions are opposite, ground subsidence occur in large scale and expands to ground level over time. Furthermore, it is acknowledgeable that ground cavity that is formed after soil run off due to seepage in deep earth, maintains steady strength and stays on the ground level for long period.

• Journal of the Korean Institute of Intelligent Systems
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• v.20 no.1
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• pp.140-145
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• 2010

In this work, to theoretically analyze the nonlinear charging characteristic, a Radial Basis Function Neural Network (RBFNN) is adopted. Based on the RBFNN, an charging characteristic tendency of a Linear Type Magnetic Flux Pump (LTMFP) is analyzed. In the paper, we developed the LTMFP that generates stable and controllable charging current and also experimentally investigated its charging characteristic in the cryogenic system. From these experimental results, the charging current of the LTMFP was also found to be frequency dependent with nonlinear quality due to the nonlinear magnetic behaviour of superconducting Nb foil. On the whole, in the case of essentially cryogenic experiment, since cooling costs loomed large in the cryogenic environment, it is difficult to carry out various experiments. Consequentially, in this paper, we estimated the nonlinear characteristic of charging current as well as realized the intelligent model via the design of RBFNN based on the experimental data. In this paper, we view RBF neural networks as predominantly data driven constructs whose processing is based upon an effective usage of experimental data through a prudent process of Fuzzy C-Means clustering method. Also, the receptive fields of the proposed RBF neural network are formed by the FCM clustering.

• Journal of the Korean Institute of Gas
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• v.19 no.2
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• pp.29-36
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• 2015

This study is an 1-D numerical study prior to modification of diesel engine for power plants to natural gas/diesel dual fuel engine using GT-Power with 1.5MW diesel engine for power generation. Natural gas injector was installed to intake manifold for dual fuel engine model. Effects on engine performance and characteristics were investigated when dual fuel is used in unmodified diesel engine. The analysis was done under 5 conditions from 0% to 40% of mixing rate on 720RPM engine speed. As a result of research, the engine performance was decreased as increasing ratio of natural gas. Engine brake power was decreased by 18.4% under 40% mixing rate condition. To clarify the reason, effects of injection timing and period were evaluated with DOE method. Considering this result, optimization was done for these parameters. Also, comparison between performances of dual fueled engine and diesel engine was made after optimizing the timing of injection by DOE method. As a result, engine brake power was decreased by 8.55% under mixing rate 40% condition showing 12.5% improvement.

• Journal of Distribution Science
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• v.14 no.4
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• pp.111-116
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• 2016

Purpose - A lot of preceeding studies have focused on the pains that might be felt in spending money, that is an important role in getting psychological safety. Money and social resources can be all for consumers, and they are likely to form a substituting relationship with one another. Being motivated by the idea that spending money for others can come with joy if one's objective is accomplished by social support, this paper aimed to explore the mediating role of the perceived money importance and gratitude in the relationship between social support and pleasure in spending for other-gift. Research Design, Data, and Methodology - In this empirical study, the experimental group is expected to receive social support while the control group is composed of those who are usually indulged in reminiscences of their sweethearts. From the college students, 160 experiment participants were selected and 80 participants of them were assigned to control group as well as to experimental group respectively at random. Empirical study for each of the two groups was performed respectively by means of questionnaire survey. Experimental group data and control group data were combined together to be used for testing hypotheses. Linear structural equation model in Amos was used to verify the hypotheses, and Bootstrap was also used to examine whether there were the mediating roles of the perceived money importance and gratitude or not. Results - From the empirical study, following conclusions could be drawn: First, social support of others makes one perceive the importance of money less; Second, social support of others makes one perceive gratitude to others; Third, less perceived importance of money and gratitude to others can make one feel pleasure in spending for other-gift; and Fourth, less perceived importance of money and gratitude to others can partially mediate the effect of social support from others on the pleasure in spending for other-gift. Conclusions - The outcomes of this study might offer theoretic and managerial implications as follow: Even though many hitherto studies have asserted that spending money usually comes with pains, this study discovered that social support might reduce perceived the importance of money but make others feel gratitude and, thus, one would feel joy in spending money to buy gift for others, and made a contribution to the progress of the theory of pleasure in spending for other-gift. This paper also made contributions toward the development of emotion marketing theory by showing that the effect of social support on the pleasure in spending for other-gift could be partially mediated by the perceived the importance of money and gratitude to others. Based on the above conclusions, it may be affirmed that marketers should help consumers perceive the importance of money less, and help feel gratitude to others by pointing up the support of others to consumers in an attempt to accelerate spending for other-gift.

• Korean Chemical Engineering Research
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• v.59 no.3
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• pp.417-428
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• 2021

The combustion characteristics of anthracite, which follow a complex process with low reactivity, must be considered through the dynamic behavior of circulating fluidized bed (CFB) boilers. In this study, computational fluid dynamics (CFD) simulation was performed to analyze the combustion characteristics of anthracite in a pilot scale 0.1 MW_th Oxy-fuel circulating fluidized bed (Oxy-CFB) boiler. The 0.1MW_th Oxy-CFB boiler is composed of combustor (0.15 m l.D., 10 m High), cyclone, return leg, and so on. To perform CFD analysis, a 3D simulation model reactor was designed and used. The anthracite used in the experiment has an average particle size of 1,070 ㎛ and a density of 2,326 kg/m³. The flow pattern of gas-solids inside the reactor according to the change of combustion environment from air combustion to oxygen combustion was investigated. At this time, it was found that the temperature distribution in air combustion and oxygen combustion showed a similar pattern, but the pressure distribution was lower in oxygen combustion. addition, since it has a higher CO₂ concentration in oxygen combustion than in air combustion, it can be expected that carbon dioxide capture will take place actively. As a result, it was confirmed that this study can contribute to the optimized design and operation of a circulating fluidized bed reactor using anthracite.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.6
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• pp.142-149
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• 2019

In this study, the past erosion history and current status in the CHUNG-UI beach of Eulwang-dong, Jung-gu, Incheon-Si, South Korea were investigated and analyzed the wave with wave-induced current to investigate the causes of coastal erosion. As a result, the significant wave height ($H_{1/3}$) was in the range of 0.07~1.57 m and the mean value was 0.21 m. The maximum wave height ($H_{max}$) was in the range of 0.02-4.76m and the mean value was 0.27m. The vertical wave height and cycles were estimated through numerical model experiments of wave transformation. The 50-year frequency design wave height ranged from 0.82m to 3.75m. As a result of the experiment of wave-induced current, wave-induced current in the CHUNG-UI beach was decreased after the installation of the Detached breakwater and the Jetty. On the other hand, when the crest elevation was increased up to 5 m, there was no significant change, but when the crest elevation was increased to 8m, strong wave-induced current occurred around the submerged breakwaters due to lowered depth of water. In addition, the main erosion of the CHUNG-UI beach is due to the intensive invasion of the wave characteristics coming from the outer sea into the white sandy beach. The deformation of the wave centered on the front of the sandy beach caused additional longshore currents flowing parallel to the sandy beach and rip currents in the transverse direction, thus confirming that the longshore sediment was moved out of the front and out of the sea. The results of this study can be used as preliminary data for the recovery of the sand and the selection of efficient erosion prevention facilities.

• Journal of the Korea Society for Simulation
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• v.28 no.2
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• pp.1-14
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• 2019

A full scale hydrodynamic simulation that requires an accurate reproduction of shock-induced detonation was conducted for design of an energetic component system. A detailed hydrodynamic analysis SW was developed to validate the reactive flow model for predicting the shock propagation in a train configuration and to quantify the shock sensitivity of the energetic materials. The pyrotechnic device is composed of four main components, namely a donor unit (HNS+HMX), a bulkhead (STS), an acceptor explosive (RDX), and a propellant (BPN) for gas generation. The pressurized gases generated from the burning propellant were purged into a 10 cc release chamber for study of the inherent oscillatory flow induced by the interferences between shock and rarefaction waves. The pressure fluctuations measured from experiment and calculation were investigated to further validate the peculiar peak at specific characteristic frequency (${\omega}_c=8.3kHz$). In this paper, a step-by-step numerical description of detonation of high explosive components, deflagration of propellant component, and deformation of metal component is given in order to facilitate the proper implementation of the outlined formulation into a shock physics code for a full scale hydrodynamic simulation of the energetic component system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.4
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• pp.619-625
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• 2019

It is difficult to design because of the plug-holing phenomenon in which the amount of smoke discharged from the vertical vent is smaller than the designed amount of smoke. In this study, the effect of cross-sectional area ratio of tunnel and natural ventilation and heat release rate of fire source on plug-holing phenomenon occurring in natural ventilation system was experimentally analyzed. In the experiment model reduced to 1/20 size, the aspect ratio of the tunnel and the vertical vent was fixed, and the influence on the plug-holing phenomenon was confirmed by varying the sectional area ratio of the tunnel and the vertical vent. Experimental results show that the plug-holing phenomenon is caused by the comparison of the smoke boundary layer temperature with the temperature in the vertical vents, and the flow and temperature distribution characteristics under the vertical vents are changed as the cross-sectional area ratio of the tunnel and vertical vents increases. The plug-holing phenomenon is affected by the cross-sectional area ratio between the tunnel and the vertical ventilation. The greater the cross-sectional area ratio, the greater the probability of plug-holing.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.5
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• pp.20-26
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• 2019

Where hydraulic structures are to be installed over the entire width of a river or stream, usually a bed protection structure is to be installed. However, a local scour occurs in which the river bed downstream of the river protection system is eroded due to the influence of the upstream flow characteristics. This local scour is dominant in the flow and turbulence characteristics at the boundary of the flow direction and in the material of the bed materials, and may gradually become dangerous over time. Therefore, in this study, we compared the turbulence patterns in the local scour hole at the downstream of the river bed protection with the results of the analysis of the mobile bed experiment, and compared with the application of OpenFoam, a three dimensional numerical analysis model. The distribution of depth-averaged relative turbulence intensities along the flow direction was analyzed. In addition to this result, the stabilization of scour hole was compared with the bed shear stress and Shields parameter, and the results were compared by changing the initial turbulent flow conditions. From the results, it was confirmed that the maximum depth of generation of the three-stage was dominantly developed by the magnitude of depth-averaged relative turbulence intensity rather than the mean flow velocity. This result also suggests that design, construction or gate control are needed to control the depth-averaged relative turbulence intensities in order to reduce or prevent the local scour faults that may occur in the downstream part of the bed protection.