• Applied Biological Chemistry
• /
• v.19 no.4
• /
• pp.227-232
• /
• 1976

This experiment was carried out to investigate the effects of red pepper powder on its enzyme production and growth of Aspergillus oryzae. In this report, Aspergillus oryzae A and G strains were cultured to the wheat bran and Czap다 Dox liquid media containing red pepper powder. And their enzyme activity. dry mycelial weight, pH and acidity were determined respectively. The results obtained were as follows. 1. In the case of protease reaction on the substrate without salt, the addition of red pepper powder ranging from 0.05 to 30 per cent to the wheat bran medium showed the increased neutral and alkali protease production in comparison with the control. However, the acid protease production were decreased by the addition of red pepper powder in the case of Aspergillus oryzae G strain. 2. In the case of protease reaction on the substrate with 10 per cent of salt, wheat bran medium containing 0.05 to 10per cent of red pepper powder showed the high protease production in comparison with the control. 5. As the amount of red pepper added to the Czapek-Dox lipuid culture was increased, the dry mycelial weight produced by Aspergillus oryzae A and G strains were also increased. And the dry mycelial weight produced by Aspergillus oryzae A strain was much more than that of Aspergillus oryzae G strain. 6. The addition of red pepper powder brought the fall of pH in Czapek-Dox liquid medium. 7. By the increase of red pepper powder concentration, the acidity in Czapek-Dox liquid culture were increased. And the increase ratio in the case of Aspergillus oryzae G strain was more remarkable than that of Aspergillus oryzae A strain.

• Journal of Fisheries and Marine Sciences Education
• /
• v.8 no.1
• /
• pp.41-47
• /
• 1996

The fishing experiment was carried out in order to analyses the mesh selectivity for trawl cod - end in the Southern Korean Sea and East China Sea from June,1991 through December, 1992. The mesh selectivity was examined for the five kinds with opening mesh size, of which having mesh s izes 51.2mm, 70.2mm, 77.6mm, 88.0mm and 111.2mm. In this report, mesh selectivity were taken analyses trawl cod - end for Priacanthus macracanthus. The main results obtained were as follows : 1. The relation equations between total length(TL) and body height(BH), body girth(BG) and body weight(BW) were BH=0.256 TL+2.963, BG=0.620 TL+8.650 and $BW=7.34{\times}10^{-5}TL^{2.6956}$, respectively. 2. 50% selection length and selection factor of trial cod - end with opening mesh size having 51.2mm, 70.2mm, 77.6mm, 88.0mm were 94.8mm, 1.85 ; 159mm, 2.27 ; 204mm, 2.09 ; 217.3mm, 2.18, respectively. 3. Range of opening mesh size from 51.2mm to 88.0mm, parameters of master curve were slope 2.70, intercept - 5.7 and selection factor 2.11.

• Geophysics and Geophysical Exploration
• /
• v.12 no.1
• /
• pp.41-48
• /
• 2009

Natural magnetic fields are attenuated by electrically conductive water. For that reason, marine magnetotelluric surveys have collected data at long periods (1000-100 000 s). The mantle structure has been the main target of seafloor magnetotelluric measurements. To ascertain crustal structure, however, electromagnetic data at shorter periods are important, e.g. in investigations of megathrust earthquake zones, or in natural resource surveys. To investigate of the former, for example, electromagnetic data for periods of less than 1000 s are necessary. Because no suitable ocean bottom electromagnetometer (OBEM) has been available, we have developed a small OBEM and ocean bottom electrometer (OBE) system with a high sample rate, which has an arm-folding mechanism to facilitate assembly and recovering operations. For magnetic observation, we used a fluxgate sensor. Field observations were undertaken to evaluate the field performance of our instruments. All instruments were recovered and their electromagnetic data were obtained. Results of the first experiment show that our system functioned well throughout operations and observations. Results of other field experiments off Tottori support the claim that the electromagnetic data obtained using the new OBEM and OBE system are of sufficient quality for the survey target. These results suggest that this device removes all instrumental obstacles to measurement of electromagnetic fields on the seafloor.

• Journal of Haehwa Medicine
• /
• v.10 no.1
• /
• pp.395-415
• /
• 2001

A general concept of Geun includes the soft organizations such as a muscle, a myofascia, a tendon and a ligament, cartilages and nerves that surround it. The main function of it is a movement of the body such as a flextion and a extension, a connection of the joints, and a construction of the body shape. Lately, we consider Geun important little by little, develop a variety of the therapeutic measures which make use of it, and apply it to the treatment in the oriental medicine, And the therapeutic measures which utilize Geun will be developed from now on. Therefore, this study reviewed and analyzed the direction of the research referred to Geun in the oriental medicine and the tendency to the experiment to grow the study referred to Geun in oriental medicine continuously and developed the new direction. This study collected 44's theses reported in the oriental medical journal and analyzed in accordance with the journal, time, form and topics. The following results were obtained in this study; 1. In the theses referred to Geun, the journal of Korean acupuncture & moxibustion society and of Korean oriental medicine have the most of them(11's theses) and at the next the journal of oriental rehabilitation medicine has 9's theses. 2. By analyzing the form of the theses, these were classified into the theoretical, experimental and clinical studies. The theoretical these occupied 39%, the experimental theses 54%, and the clinical theses 7%. 3. By analyzing the theses referred to Geun, these were classified into the muscular abnormality - such as a powerlessness, a injury, a atrophy, a disorder, and a pain - the theory of muscles along the regular meridians, the muscular organization, the therapeutic measures, the manipulation and so on. 4. The theses of the muscular abnormality occupied 30% of them referred to Geun and the theory of muscles along the regular meridians 20%, the muscular organization 12%, the therapeutic measures 12%, and the manipulation 8%. According to the above results, the study of the new field referred to Geun and the clinical report are indispensable since the field is limited and the tendency is theoretical and experimental.

• Journal of Life Science
• /
• v.25 no.7
• /
• pp.748-756
• /
• 2015

The report explores the possibility that the single extract of natural substances and the mixture of single extracts of natural substances create a synergy effect to increase the antimicrobial activity. It also compares the previous researches to find out if the natural medicinal herbs’ extract has antimicrobial activity on dandruff causative organism, Malassezia furfur. For the experiment on the mixture of single extracts of natural substances, the results are like following: 1. Staphylococcus aures’s antibacterial activity is resistant to mixture of three natural substances. 2. Escherichia coil’s antibacterial activity is resistant to mixture with Coptidis rhizoma. 3. Candida albicans’ antifungal activity is resistant to mixture with Chinese galls, which means the different results may be expected when tested with each germ. 4. On the other hand, Malassezia furfur has no antifungal activity on the single extract of natural substances and shows weak antifungal activity, whose diameter is 3.20 mm when tested with the mixture of 50% of Coptidis rhizoma and 50% of Phytoncide. The result is totally different from the one on the same eumycetes, C. albicans. That is because M. furfur has more lipophilic chemicaled cell walls than C. albicans does and it also consists of lamella layer, inner plasma membrane and intermediate multiple layers.

• Journal of Marine Bioscience and Biotechnology
• /
• v.1 no.3
• /
• pp.186-192
• /
• 2006

Short interspersed repetitive elements (SINEs) are dispersed throughout eukaryotic genomes. These SINEs have been shown to be excellent phylogenetic markers for the closed related species. In this report, we isolated two novel families of SINEs from the mud loach. The two SINE families, mlSINE-L and mlSINE-S, have genomic lengths of about 410bp and 270bp, respectively. 5' and 3' ends of the SINE families are well conserved and highly homologous to each of corresponding ends of RSg-1 and SmaI SINEs. Phylogenetic analysis shows that mlSINEs are unique to the mud loach. A dot blot hybridization experiment shows that mlSINE-L has an estimated copy number of $1{\times}10^3$ per $2{\times}10^9bp$ (2.8 pg) and is more frequently distributed at nuclear matrix attachment regions (MARs) than loop DNAs. The result suggests that mlSINEs may preferentially integrate in or near MARs.

• Journal of the Korea Institute of Building Construction
• /
• v.20 no.5
• /
• pp.399-407
• /
• 2020

The aim of the research is to provide the boundary conditions for segregation of normal strength grade and high fluidity concrete mixture (so called mid-fluidity concrete) with rheology parameters. Since the normal strength grade concrete mixture has a relatively high water-to-cement ratio and no SCMs, it is easy to be segregated when superplasticizer is added. Hence, to achieve the mid-fluidity concrete of normal strength grade and high fluidity, preventing segregation of the mixture is inevitable. In this research, using two superplasticizers with different solid concentrations, the flow behaviors and rheological behaviors were assessed by increasing fluidity until the segregation happened. According to the experiment in this research, an unusual behavior in rheology parameters was observed when the concrete mixture started to be segregated. From this results and report, it is expected to contribute on the definition of segregation with rheological test methods.

• Korean Journal of Agricultural Science
• /
• v.39 no.4
• /
• pp.515-523
• /
• 2012

Recent development and enlargement of reclaimed lands along the sea shores required to explore alternatives to existing crops, which are suitable to grow well and to declare higher profits. The objectives of this study were to investigate yield-related parameters for development of new leafy vegetables, and to screen some candidates among New Zealand spinach genotypes which were collected in Korea. Initially all the collected genotypes were grown for 7 weeks in a greenhouse of the experiment field in Chungnam National University, and then transplanted into the field to measure morphological or physiological parameters (plant height, branch number, stem diameter, chlorophyll content, and maximum canopy diameter), and yield-related parameters (leaf number, leaf length, leaf width, fresh weight and dry weight). Those parameters were quantitatively measured at 1, 5 or 9 weeks after transplanting (WAT). Parameters exhibiting statistically significant difference among the accessions were plant height, branch number, maximum canopy diameter, chlorophyll content, and leaf length. In contrast to highly correlated parameters with fresh and dry weight obtained in salt-affected soil in the earlier report, fresh weight was correlated significantly with leaf length (r=0.72), leaf width (r=0.64), and canopy diameter(r=0.66), while dry weight was correlated significantly with plant height (r=0.46), leaf length (r=0.72), leaf width (r=0.73), fresh weight (r=0.79), chlorophyll content (r=0.47), and canopy diameter (r=0.87). Based on the significantly correlated with yield parameters, the candidate accessions which were ranked in top statistical groups include CNU06A01, CNU06A13, CNU06A26, CNU06A35, CNU06A38, and CNU06A55. In order to be cultivated in reclaimed lands, it is necessary to screen out salt tolerant accessions among the above high-yielding genotypes.

• Solar Energy
• /
• v.19 no.4
• /
• pp.45-53
• /
• 1999

This report Introduces a total heat exchanger in a solar air-conditioning system using Lithium Chloride(LiCl) solution. The hot and humid outside air is cooled and dehumidified by LiCl solution that is sprayed on the packed layer of the total heat exchanger. LiCl solution once diluted is concentrated again in a regenerator using solar energy. Three types as the packed materials were used in this experiment and the dehumidification performance was evaluated by the value of $k_xa(kg/h{\cdot}m^3{\cdot}{\Delta}x)$, overall mass transfer coefficient based on a humidity ratio potential difference, the influence of inlet LiCl solution flow rate, air flow rate, packed layer height on $k_xa$ was investigated. It was found that air flow rate, LiCl solution flow rate, packed layer height for all types had a great influnce on the value of $k_xa$.

• Reproductive and Developmental Biology
• /
• v.33 no.3
• /
• pp.171-177
• /
• 2009

Spermatogonial stem cells(SSCs) only are responsible for the generation of progeny and for the transmission of genetic information to the next generation in male. Other in vitro studies have cultured SSCs for proliferation, differentiation, and genetic modification in mouse and rat. Currently, information regarding in vitro culture of porcine Germline Stem Cell(GSC) such as gonocyte or SSC is limited and is in need of further studies. Therefore, in this study, we report development of a successful culture system for gonocytes of neonatal porcine testes. Testis cells were extracted from $10{\sim}14$-day-old pigs. These cells were harvested using enzymatic digestion, and the harvested cells were purified with combination of percoll, laminin, and gelatin selection techniques. The most effective culture system of porcine gonocytes was established through trial experiments which made a comparison between different feeder cells, medium, serum concentrations, temperatures, and $O_2$ tensions. Taken together, the optimal condition was established using C166 or Mouse Embryonic Fibroblast(MEF) feeder cell, Rat Serum Free Medium(RSFM), 0% serum concentration, $37^{\circ}C$ temperature, and $O_2$ 20% tension. Although we discovered the optimal culture condition for proliferation of porcine gonocytes, the gonocyte colonies ceased to expand after one month. These results suggest inadequate acquirement of ingredients essential for long term culture of porcine GSCs. Consequently, further study should be conducted to establish a successful long-term culture system for porcine GSCs by introducing various growth factors or nutrients.