• Title/Summary/Keyword: Expansion Method

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Establishment of intestinal organoids from small intestine of growing cattle (12 months old)

  • Kang Won, Park;Hyeon, Yang;Min Gook, Lee;Sun A, Ock;Hayeon, Wi;Poongyeon, Lee;In-Sul, Hwang;Jae Gyu, Yoo;Choon-Keun, Park;Bo Ram, Lee
    • Journal of Animal Science and Technology
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    • v.64 no.6
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    • pp.1105-1116
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    • 2022
  • Recently, we reported the robust in vitro three-dimensional (3D) expansion of intestinal organoids derived from adult bovine (> 24 months) samples. The present study aimed to establish an in vitro 3D system for the cultivation of intestinal organoids derived from growing cattle (12 months old) for practical use as a potential alternative to in vivo systems for various purposes. However, very few studies on the functional characterization and 3D expansion of adult stem cells from livestock species compared to those from other species are available. In this study, intestinal crypts, including intestinal stem cells, from the small intestines (ileum and jejunum) of growing cattle were isolated and long-term 3D cultures were successfully established using a scaffold-based method. Furthermore, we generated an apical-out intestinal organoid derived from growing cattle. Interestingly, intestinal organoids derived from the ileum, but not the jejunum, could be expanded without losing the ability to recapitulate crypts, and these organoids specifically expressed several specific markers of intestinal stem cells and the intestinal epithelium. Furthermore, these organoids exhibited key functionality with regard to high permeability for compounds up to 4 kDa in size (e.g., fluorescein isothiocyanate [FITC]-dextran), indicating that apical-out intestinal organoids are better than other models. Collectively, these results indicate the establishment of growing cattle-derived intestinal organoids and subsequent generation of apical-out intestinal organoids. These organoids may be valuable tools and potential alternatives to in vivo systems for examining host-pathogen interactions involving epithelial cells, such as enteric virus infection and nutrient absorption, and may be used for various purposes.

A Study on the Site Selection Method for the Creation of a Flood Buffer Section Considering the Nature-based Solution - Case Study from Upstream of Daecheong Dam to Downstream of Yongdam Dam (자연성기반기술의 홍수완충구간 조성을 위한 입지 선정 방법에 관한 연구 - 대청댐 상류부터 용담댐 하류구간 사례 연구)

  • Ji, Un;Jang, Eun-kyung;Bae, Inhyeok;Ahn, Myeonghui;Bae, Jun
    • Ecology and Resilient Infrastructure
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    • v.9 no.3
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    • pp.131-140
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    • 2022
  • The magnitude and frequency of extreme floods are increasing owing to the effects of climate change. Therefore, multipurpose flood management techniques incorporating nature-based solutions have been introduced to mitigate the limitations of flood management and river design methods relying on existing observation data. Nature-based solutions to prepare for such extreme flooding events include ways to retreat the embankment, expand the floodplain, and reduce flood damage. To apply these technologies, adopting appropriate location selection methods based on various evaluation factors, such as flood damage reduction effects, sustainable ecological environments, river connectivity, and physical channel structure enhancements, should be prioritized. Therefore, in this study, the optimal location for implementing the multipurpose floodplain construction project was determined by selecting the location of the floodplain expansion with objectivity in the river waterfront area upstream of Daecheong Dam to downstream of Yongdam Dam. Through the final location determination, the Dongdaeje and Jeogokje sections were included in the optimal location considering both flood damage reduction and water environment improvement.

A Study on Technology Acceptance Plans to Expand Direct Participation in the Sports Industry (스포츠 산업의 직접 참여 확대를 위한 기술수용 방안 연구)

  • Sangho Lee;Kwangmoon Cho
    • Journal of Internet of Things and Convergence
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    • v.9 no.1
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    • pp.105-115
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    • 2023
  • This study seeks to find a way to induce users to expand their direct participation in sports through the acceptance of digital technology. From July 1 to August 30, 2022, a survey was conducted targeting home training users who applied the Internet of Things (IoT). 129 people participated in the survey through non-face-to-face self-administration method. For data processing, frequency analysis, exploratory factor analysis, reliability analysis, correlation analysis, multiple regression analysis, and 3-step mediation regression analysis were conducted using IBM's SPSS 21.0 program. The results of the study are as follows. First, in the relationship between the home training PPM model and direct participation in sports, ease appeared to have a mediating effect. In the factors of push, simple functionality showed a complete mediating effect, and inefficiency showed a partial mediating effect. Among pull factors, enjoyment and possibility of experience showed a complete mediating effect. In the mooring factors, individual innovativeness showed a complete mediating effect. Second, in the relationship between home training PPM model and direct participation in sports, usefulness showed a mediating effect. In the factors of push, simple functionality showed a complete mediating effect, and inefficiency showed a partial mediating effect. Among pull factors, enjoyment and possibility of experience showed a complete mediating effect. Among the mooring factors, individual innovativeness showed a partial mediating effect. Through this research, it is expected that the sports industry will contribute to the expansion of consumption expenditure and economic growth through the expansion of digital technologies such as NFT, Metaverse, and virtual/augmented reality.

Future of Social Work Practice - Human, human again. - (사회복지실천의 미래 - 사람과 사람 -)

  • Kim, Miok;Choi, Hyeji;Chung, Ick-Joong;Min, So-young
    • Korean Journal of Social Welfare
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    • v.69 no.4
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    • pp.41-65
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    • 2017
  • This study aimed to examine the social transition, which is often metaphorized as the Fourth Industrial Revolution, within the context of social work practice and to explore measures to improve social work practice in such transition. Four social welfare researchers held seven discussions to predict the social changes in the near future centered on the Fourth Industrial Revolution and find the corresponding development strategies in social work practice; collective autobiography method was used to analyze the discussion. The analysis ascertained hyper connectivity, the advent and expansion of new communities, diversification and individualization, and the emergence of new criteria for the assessment of one's quality of life as the distinctive qualities of the near future. It was analyzed that humans and organic materials will be interconnected through spatial and temporal transcendence and that humans liberated from labor will seek for diverse communities while the number of atomized individual will increase simultaneously. Furthermore, the rise of new order of life accompanied by both the expansion of diversification and individualization and the ecological worldview brought forth by post materialistic trend was predicted. Meanwhile, the disengagement from macroscopic context, a biased inclination towards technique orientated professionalism, and individualistic social work practices without integrity were identified as the limitations of the current social work practice. This study presented three goals for social work practice to help it overcome its current shortcomings and correspond to the social changes: first, the rearrangement of practice knowledge, technique, and value so that it is based on humans and society, which are the essence of social practice work; second, the practice, such as sharing economy, that expands the individuals' boundaries of life to the community; three, the restoration of the desirability of professional social works by examining its special nature.

A Qualitative Case Study on the Work and Welfare Service Experiences of People with Developmental Disabilities in the Occupational Rehabilitation Facilities (장애인근로사업장 발달장애인의 일과 복지서비스경험에 관한 질적 사례연구)

  • Park, Jung Im;Kim, Myung Hee
    • The Journal of the Korea Contents Association
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    • v.22 no.5
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    • pp.765-778
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    • 2022
  • This study is a qualitative case study whose aims were to investigate the work and welfare service experiences of people with developmental disabilities in the occupational rehabilitation facilities. For the purpose of this study selected as its participants 8 people with developmental disabilities whom has been worked currently in the occupational rehabilitation facilities. The profound interviews of those participants were performed and the data on the occupational rehabilitation facilities were analysed. Also, the study analysed the data in a way of investigating the respondents' statements regarding their work and welfare in the occupational rehabilitation facilities. The results of this analysis, 10 categories, 22 sub-categories and 5 key topics of the respondent's experiences were drawn out. The 5 key topics of the people with developmental disabilities in the occupational rehabilitation facilities were 'pride of the economic stability', 'happiness of work itself', 'a sense of accomplishment of the regular worker', 'recognition and exchange of the coworker and superior', 'expectation of the welfare service and the expansion of the participation in the occupational rehabilitation facilities were 'pride of the economic stability', 'happiness of work itself', 'a sense of accomplishment of the regular worker', 'recognition and exchange of the coworker and superior', 'expectation of the welfare service and the expansion of the participation in the occupational rehabilitation facilities'. Based on the results, implications for the work and welfare service of people with developmental disabilities in the occupational rehabilitation facilities were provided.

Computer vision-based remote displacement monitoring system for in-situ bridge bearings robust to large displacement induced by temperature change

  • Kim, Byunghyun;Lee, Junhwa;Sim, Sung-Han;Cho, Soojin;Park, Byung Ho
    • Smart Structures and Systems
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    • v.30 no.5
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    • pp.521-535
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    • 2022
  • Efficient management of deteriorating civil infrastructure is one of the most important research topics in many developed countries. In particular, the remote displacement measurement of bridges using linear variable differential transformers, global positioning systems, laser Doppler vibrometers, and computer vision technologies has been attempted extensively. This paper proposes a remote displacement measurement system using closed-circuit televisions (CCTVs) and a computer-vision-based method for in-situ bridge bearings having relatively large displacement due to temperature change in long term. The hardware of the system is composed of a reference target for displacement measurement, a CCTV to capture target images, a gateway to transmit images via a mobile network, and a central server to store and process transmitted images. The usage of CCTV capable of night vision capture and wireless data communication enable long-term 24-hour monitoring on wide range of bridge area. The computer vision algorithm to estimate displacement from the images involves image preprocessing for enhancing the circular features of the target, circular Hough transformation for detecting circles on the target in the whole field-of-view (FOV), and homography transformation for converting the movement of the target in the images into an actual expansion displacement. The simple target design and robust circle detection algorithm help to measure displacement using target images where the targets are far apart from each other. The proposed system is installed at the Tancheon Overpass located in Seoul, and field experiments are performed to evaluate the accuracy of circle detection and displacement measurements. The circle detection accuracy is evaluated using 28,542 images captured from 71 CCTVs installed at the testbed, and only 48 images (0.168%) fail to detect the circles on the target because of subpar imaging conditions. The accuracy of displacement measurement is evaluated using images captured for 17 days from three CCTVs; the average and root-mean-square errors are 0.10 and 0.131 mm, respectively, compared with a similar displacement measurement. The long-term operation of the system, as evaluated using 8-month data, shows high accuracy and stability of the proposed system.

Thermal Property Test of Natural Sand and Artificial Sand for the Design of Casting Process (주조공정 설계를 위한 자연주물사와 인공주물사의 열물성 시험)

  • Jeong-Ho Nam;Byoung-Jun Lee;Si-Young Kwak
    • Journal of Korea Foundry Society
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    • v.44 no.1
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    • pp.3-8
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    • 2024
  • Reducing industrial waste and improving the atmospheric environment recently have emerged as important tasks for the casting industry. Various types of artificial sand that can replace natural sand have been developed and used to solve problems that arise with the use of conventional foundry sand and to improve the foundry environment. However, only limited physical properties provided by overseas suppliers are known and in-depth comparative analyses with existing natural sand are lacking. For the design of the casting process, a wide range of thermal properties must be secured not only at room temperature but also at high temperatures. In addition, it is important to compare and analyze the differences in thermal properties between existing natural and artificial sand because changes in thermal properties require changes in the design of the casting method. In this study, the thermal behavior of the mold was analyzed through a thermal expansion test of mold blocks for natural and artificial sand., To this end, a thermal property test to evaluate thermal conductivity was performed by determining a temperature range. The results of the experiment, revealed that the thermal conductivity was 5-40% higher in artificial sand than in natural sand, and the coefficient of thermal expansion was about 15% lower for artificial sand. However, there was not a significant difference in specific heat regardless of the composition.

The Effect of Recycled Glass Sand Usage Conditions on the Mechanical Properties and Alkali-Silica Reaction of Mortar (순환유리 잔골재의 사용 조건이 모르타르의 역학적 특성 및 알칼리-실리카 반응에 미치는 영향)

  • Min-Jae Son;Gyu-Yong Kim;Hyun-Sang Choi;Gyeong-Cheol Choi;Tae-Hyeob Song
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.12 no.3
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    • pp.263-270
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    • 2024
  • In recent years, there has been an increasing amount of waste glass that cannot be recycled and is landfilled among construction and household waste. To address this issue, research has been conducted to recycle waste glass as sand, but conflicting results have emerged depending on the type of waste glass and the manufacturing method of recycled glass sand(RGS). To promote the use of RGS, it is necessary to review its performance under field conditions, such as mass production processes and real scale concrete applications. This study introduces examples of mass production system process for RGS and evaluates the effects of the usage conditions(color, content, particle size) of mass-produced RGS on the mechanical properties and alkali-silica reaction(ASR) of mortar. As a result, the mass production system process of RGS causes microcracks inside the particles, which are the cause of mortar strength reduction and ASR expansion. The number of microcracks is highest in clear RGS. Based on these results, it is proposed that RGS can be used as a replacement for natural sand with a content of less than 25 % or a particle size of less than 0.5 mm.

DAD Analysis of Yongdam Dam Watershed Using the Cell-Based Automatic Rainfall Field Tracking Methods (격자기반의 자동 강우장 탐색기법을 활용한 용담댐 유역 DAD분석)

  • Song, Mi-Yeon;Jung, Kwan-Sue;Lee, Gi-Ha;Kim, Yeon-Su;Shin, Young-A
    • Journal of the Korean Association of Geographic Information Studies
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    • v.17 no.3
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    • pp.68-81
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    • 2014
  • This study aims to apply and evaluate the automatic DAD analysis method, which is able to establish the depth-area relationship more efficiently and accurately for point-to-areal rainfall conversion. First, the proposed automatic DAD analysis method tracks the expansion route of area from the storm center, and it is divided into Box-tracking, Point-tracking, Advanced point-tracking according to tracking method. After applying the proposed methods to 10 events occurred in Yongdam-watershed area, we confirmed that the Advanced point-tracking method makes it possible to estimate the maximum average areal rainfal(MAAR) more accurately with consideration of the storm movement and the multi-centered storm. In addition, Advanced point-tracking could reduce the errors of the estimated MAAR induced by increasing the area because it can estimate MAAR for each storm center and compare them at the same time. Finally, the DAD curve for the study area could be derived based on the DAD analysis of the selected 10 events.

Study on the Business Process Modeling scheme using the Context Analysis methodology (상황 분석 방법론을 적용한 효율적 비즈니스 프로세스 모델링 방안에 관한 연구)

  • You, Chi-Hyung;Sang, Sung-Kyung;Kim, Jung-Jae;Na, Won-Shik
    • Journal of Digital Contents Society
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    • v.9 no.4
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    • pp.661-667
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    • 2008
  • The dynamics of business cycles has been changed by the macroscopic economic forces because of the introduction of new technical know-how each year. These the dynamics of business has a significant influence on the investment of enterprise in the information communication field. Today, the most important goal of the IT investment is simply not to lower the production cost any more, but to improve the usefulness for the customers and partners in order to obtain the optimized mass products. Therefore, the enterprises have been concentrating their all abilities on the automation, integration, and optimization of business process using BPM. In addition, they are concentrating their efforts on the business expansion by approaching the technical aspect using RFID application system. However, in order to accomplish a successful enterprise ability, the technical view, business process view, and organization view must be considered together. We suggested the method considering organization view, via the technical element, i.e., RFID system for approaching the business process. Furthermore, we tried the optimization of assignment using Context Analysis methodology and proposed the method to reduce the element with respect to the time, human, and expense by applying the Case Study method that minimizes the iteration times through the transmitted processing procedure and type. The proposed method gave us the expectation that it will bring out the innovative improvement with respect to the time, expense, quality, and customer's satisfaction in the process from the analysis of business process to the analysis and design of system.

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