• Journal of Animal Reproduction and Biotechnology
• /
• v.37 no.2
• /
• pp.136-143
• /
• 2022

Recent progress has been made to establish intestinal organoids for an in vitro model as a potential alternative to an in vivo system in animals. We previously reported a reliable method for the isolation of intestinal crypts from the small intestine and robust three-dimensional (3D) expansion of intestinal organoids (basal-out) in adult bovines. The present study aimed to establish next-generation intestinal organoids for practical applications in disease modeling-based host-pathogen interactions and feed efficiency measurements. In this study, we developed a rapid and convenient method for the efficient generation of intestinal organoids through the modulation of the Wnt signaling pathway and continuous apical-out intestinal organoids. Remarkably, the intestinal epithelium only takes 3-4 days to undergo CHIR (1 µM) treatment as a Wnt activator, which is much shorter than that required for spontaneous differentiation (7 days). Subsequently, we successfully established an apical-out bovine intestinal organoid culture system through suspension culture without Matrigel matrix, indicating an apical-out membrane on the surface. Collectively, these results demonstrate the efficient generation and next-generation of bovine intestinal organoids and will facilitate their potential use for various purposes, such as disease modeling, in the field of animal biotechnology.

• KIPS Transactions on Software and Data Engineering
• /
• v.11 no.1
• /
• pp.35-40
• /
• 2022

In this paper, we propose a method of constructing clinical knowledge with clinical concept relations and predicting diseases based on a deep learning model to support clinical decision-making. Clinical terms in UMLS(Unified Medical Language System) and cancer-related medical knowledge are classified into five categories. Medical related documents in Wikipedia are extracted using the classified clinical terms. Clinical concept relations are established by matching the extracted medical related documents with the extracted clinical terms. After deep learning using clinical knowledge, a disease is predicted based on medical terms expressed in a query. Thereafter, medical terms related to the predicted disease are selected as an extended query for clinical document retrieval. To validate our method, we have experimented on TREC Clinical Decision Support (CDS) and TREC Precision Medicine (PM) test collections.

• Journal of the Korea Convergence Society
• /
• v.12 no.11
• /
• pp.91-97
• /
• 2021

This paper uses a data extension technique originally designed for the rule refinement problem to handling incomplete data. This technique is characterized in that each event can have a weight indicating importance, and each variable can be expressed as a probability value. Since the key problem in this paper is to find the probability that is closest to the missing value and replace the missing value with the probability, three different algorithms are used to find the probability for the missing value and then store it in this data structure format. And, after learning to classify each information area with the SVM classification algorithm for evaluation of each probability structure, it compares with the original information and measures how much they match each other. The three algorithms for the imputation probability of the missing value use the same data structure, but have different characteristics in the approach method, so it is expected that it can be used for various purposes depending on the application field.

• Journal of the Society of Disaster Information
• /
• v.19 no.1
• /
• pp.128-137
• /
• 2023

Purpose: This study proposes a way to improve quality and increase safety by studying a method that allows the system scaffolding, which is partly used for high-altitude work at construction sites, to be applied to various types of structures in shipbuilding and plant industry sites. Method: A fact-finding survey was conducted targeting 8 companies, and a plan was established that reflected the opinions of the field survey and the practitioners and experts by analyzing the literature and the Internet survey. Result: Through analysis of related patents, FGI with companies, etc., we discovered the possibility of application expansion through research and development of materials that can be applied to various types and improvement plans for enhancing the professionalism of construction companies. Conclusion: System scaffolding has a high importance in terms of safety, but related research around the world is sluggish. Therefore, it will be possible to meet the demands for quality and safety by enhancing the software expertise and developing and researching user materials that can be applied to various types.

• Journal of the Society of Disaster Information
• /
• v.19 no.3
• /
• pp.510-518
• /
• 2023

Purpose: This study compares and analyzes the characteristics and progress of existing voice phishing and new voice phishing to present a basic policy plan to prepare countermeasures against new voice phishing based on psychological descriptions. Method: The criminal progress and characteristics of the two were compared and analyzed through damage cases on various portal sites centered on voice phishing crime scenarios. Result: As a result of analyzing the progress of the third stage of new voice phishing, the scenario of new voice phishing that can deceive victims was written more carefully and the scope of the crime was expanded. In the crime execution stage, the victim was socially isolated, reducing the victim's judgment ability, making it more difficult for investigative agencies to investigate, and in the final stage, the continuity and expansion of criminal damage such as extortion of money and valuables are shown. Conclusion: There were differences in the target and scope of the crime and the method of the crime strategy between the two, and the possibility of damage is much greater, so a more efficient response strategy should be prepared.

• Journal of the Korean GEO-environmental Society
• /
• v.24 no.5
• /
• pp.5-12
• /
• 2023

Recently, due to urban development and expansion, construction plans have been increasing adjacent to existing tunnel structures such as subways, roads, and large pipelines. Structural plans adjacent to existing tunnels have different effects on tunnel stability depending on the construction method, degree of proximity, and location of new structures. In particular, the pressure water tunnel shows a very large difference from other road tunnels and railway tunnels in geotechnical characteristics and operation characteristics. Therefore, it is necessary to review the safety zone due to adjacent construction in consideration of the geotechnical characteristics of the water tunnel and the new sturure construction method. In this study, the existing tunnel safety zone standards were investigated. A stability evaluation performed numerical analysis considering the deterioration of concrete lining in operation and the characteristics of water tunnel. In addition, the impact of vibration caused by pile construction and blasting excavation of new structures was reviewed. Based on this, a pressure water tunnel safety zone was proposed in consideration of adjacent construction.

• Journal of the Korean Geotechnical Society
• /
• v.24 no.7
• /
• pp.75-80
• /
• 2008

When a saturated clay is sampled in an undisturbed manner from a bore hole, the sample extends vertically and shrinks horizontally under undrained conditions due to stress release. The conventional consolidation test specimen is trimmed from the expanded sample so that its diameter is equal to the inner diameter of the consolidation test ring, and this test procedure does not reproduce the actual consolidation behavior. The measurement of sample extension was conducted by means of overcoring method showed that the extension strains were 1 to 2%. To simulate the in-situ consolidation behavior, we proposed the consolidation test method that uses a specimen with a slightly smaller diameter than the inside diameter of consolidometer so that the specimen expands laterally to the inside of the ring.

• Journal of the Korean Geotechnical Society
• /
• v.24 no.8
• /
• pp.111-123
• /
• 2008

Geotechnical engineering problems are characterized by many sources of uncertainty. Some of these sources are connected to the uncertainties of soil properties involved in the analysis. In this paper, a numerical procedure for a probabilistic analysis that considers the spatial variability of soil properties is presented to study the response of spatially random soil. The approach integrates a commercial finite difference method and random field theory into the framework of a probabilistic analysis. Two-dimensional non-Gaussian random fields are generated based on a Karhunen-$Lo{\grave{e}}ve$ expansion in a fashion consistent with a specified marginal distribution function and an autocorrelation function. A Monte Carlo simulation is then used to determine the statistical response based on the random fields. A series of analyses were performed to study the effects of uncertainty due to the spatial heterogeneity on the settlement and bearing capacity of a rough strip footing. The simulations provide insight into the application of uncertainty treatment to the geotechnical problem and show the importance of the spatial variability of soil properties with regard to the outcome of a probabilistic assessment.

• Composites Research
• /
• v.19 no.1
• /
• pp.15-21
• /
• 2006

It is very difficult to make complex 3 dimensional curved-shape composite laminates using the advanced unidirectional composite prepregs. This study shows development process of subscale composite parabolic antenna reflector using unidirectional AS4/PEEK prepreg tapes. The AS4/PEEK thermoplastic composite materials are known to have good thermal and chemical stabilities in addition to their high specific strength and modulus. Various lamination methods were investigated through finite element analyses to make up the laminate design of the reflector. The automated fiber placement method was used to fabricate the reflector. The thermal expansion test using full-bridge strain gage circuits was done to verity the performance of the composite product.

• Composites Research
• /
• v.20 no.3
• /
• pp.37-42
• /
• 2007

Tensile residual stress is occurred by difference of coefficients of thermal expansion between fiber and matrix is one of the serious problems in metal matrix composite(MMC). TiNi alloy fiber was used to solve the problem of the tensile residual stress as the reinforced material. TiNi alloy fiber improves the tensile strength of composite with occurring of compressive residual stress in the matrix by its shape memory effect. A hot press method was used to create the optimal fabrication condition for a Shape Memory Alloy(SMA) composite. The bonding effect of the matrix and the reinforcement within the SMA composite by the hot press method was strengthened by cold rolling. In addition, acoustic emission technique was used to quantify the microscopic damage behavior of cold rolled TiNi/A16061 shape memory alloy composite at low temperature. The damage degree for the specimen that underwent thermal shock cycles was also discussed.