• Title/Summary/Keyword: Expansion Method

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Vitrification of mouse embryos using the thin plastic strip method

  • Ryu, Eun Kyung;Hur, Yong Soo;Ann, Ji Young;Maeng, Ja Young;Park, Miji;Park, Jeong Hyun;Yoon, Jung;Yoon, San Hyun;Hur, Chang Young;Lee, Won Don;Lim, Jin Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.39 no.4
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    • pp.153-160
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    • 2012
  • Objective: The aim of this study was to compare vitrification optimization of mouse embryos using electron microscopy (EM) grid, cryotop, and thin plastic strip (TPS) containers by evaluating developmental competence and apoptosis rates. Methods: Mouse embryos were obtained from superovulated mice. Mouse cleavage-stage, expanded, hatching-stage, and hatched-stage embryos were cryopreserved in EM grid, cryotop, and TPS containers by vitrification in 15% ethylene glycol, 15% dimethylsulfoxide, 10 ${\mu}g/mL$ Ficoll, and 0.65 M sucrose, and 20% serum substitute supplement (SSS) with basal medium, respectively. For the three groups in which the embryos were thawed in the EM grid, cryotop, and TPS containers, the thawing solution consisted of 0.25 M sucrose, 0.125 M sucrose, and 20% SSS with basal medium, respectively. Rates of survival, re-expansion, reaching the hatched stage, and apoptosis after thawing were compared among the three groups. Results: Developmental competence after thawing of vitrified expanded and hatching-stage blastocysts using cryotop and TPS methods were significantly higher than survival using the EM grid (p<0.05). Also, apoptosis positive nuclei rates after thawing of vitrified expanded blastocysts using cryotop and TPS were significantly lower than when using the EM grid (p<0.05). Conclusion: The TPS vitrification method has the advantages of achieving a high developmental ability and effective preservation.

The Utilization of Photo Balloon System to GSIS Construction for Build-up a Group of Villages (집단마을 조성사업용 GSIS 구축을 위한 기구사진체계의 활용)

  • Lee, Jae-Kee;Lee, Hyun-Jik;Cho, Jae-Ho
    • Journal of Korean Society for Geospatial Information Science
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    • v.2 no.2 s.4
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    • pp.67-79
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    • 1994
  • Recently, the rapid economic growth has caused urban problems due to the increase of urban population and the rapid expansion as a result of exodus phenomenon in rural territories. In order to solve the problems, we need to perform rational and effective plans for national land development. Generally, we need speedy and accurate topographical information of the object area to go ahead with balanced national land development. Acquisition method for topographical data include utilization of existing geographic information such as national base maps and related thematic maps and grasping of accurate present condition of object area using aerial photographies or satellite images. When the object area is small, existing methods for acquiring topographical information reveals imitations in accuracy, speed and economic aspect. Therefore, development of a new method is in order. As a result of this study, we recogized that Photo Balloon System to be manufactured and digital ortho-map to be generated could be used to obtain a topographical information for small areas.

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The Cell Survival and Differentiation after Transplantation, Which Harvest from Adult Rat Brain by High-speed Centrifugation Method

  • Kim, Jong-Tae;Yoo, Do-Sung;Woo, Ji-Hyun;Huh, Pil-Woo;Cho, Kyung-Sock;Kim, Dal-Soo
    • Journal of Korean Neurosurgical Society
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    • v.38 no.2
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    • pp.121-125
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    • 2005
  • Objective : Many recent reports have shown that the mature mammalian brain harbors multipotent stem cells, rendering the brain capable of generating new neurons and glia throughout life. Harvested stem cells from an adult rat are transplanted in order to evaluate the cell survival and differentiation. Methods : Using a percoll gradient with a high speed centrifugation method, we isolate neural stem/progenitor cells were isolated from the subventricular zone[SVZ] of a syngeneic adult Fisher 344 rats brain. For 14days expansion, the cultured cells comprised of a heterogeneous population with the majority of cells expressing nestin and/or GFAP. After expanding the SVZ cells in the presence of basic fibroblast growth factor-2, and transplanting then into the hippocampus of normal rats, the survival and differentiation of those cells were examined. For transplantation, the cultured cells were labeled with BrdU two days prior to use. In order to test their survival, the cells were transplanted into the dorsal hippocampus of normal adult Fisher 344 rats. Results : The preliminary data showed that at 7days after transplantation, BrdU+ transplanted cells were observed around the injection deposition sites. Immuno-fluorescent microscopy revealed that the cells co-expressed BrdU+ and neuronal marker ${\beta}$-tubulin III. Conclusion : The data demonstrate that the in vitro expanded SVZ cells can survive in a heterotypic environment and develop a neuronal phenotype in the neurogenic region. However more research will be needed to examine the longer survival time points and quantifying the differentiation in the transplanted cells in an injured brain environment.

Design of Zigbee Beacon Frame for High Efficiency Transmit in Home Network (홈 네트워크에서 고효율 전송을 위한 Zigbee Beacon Frame 설계)

  • Han, Kyoung-Heon;Han, Seung-Jo;Choi, Hyun-Yong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.11B
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    • pp.1373-1382
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    • 2011
  • Zigbee is communication technology most ideal because Zigbee support low power communication and wide expansion in wireless home network. However, Zigbee is not popular, because of Zigbee always has Hidden Node Problem and Transit Delay Problem. We propose new Beacon Frame structure to solve the two problem in Zigbee. New Beacon Frame structure reduces a Super Frame Duration and add a same space of a Control Frame Duration. We expect to solved method of Hidden Node Problem that exchange terminal information to using RTS/CTS. Also, We expect to solved method of Transit Delay Problem that put Beacon between Control Frame Duration and Super Frame Duration for synchronization. We confirm new Beacon Frame to solved two problem in OPNET simulation at Zigbee QoS Parameters. We measure Delay(sec) for solution degree of Transit Delay Problem, and measure Throughput(bits/sec) and Load (bits/sec) for solution degree of Hidden Node Problem.

Partial Canonical Correlation Biplot (편정준상관 행렬도)

  • Yeom, Ah-Rim;Choi, Yong-Seok
    • The Korean Journal of Applied Statistics
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    • v.24 no.3
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    • pp.559-566
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    • 2011
  • Biplot is a useful graphical method to explore simultaneously rows and columns of two-way data matrix. In particular, canonical correlation biplot is a method for investigating two sets of variables and observations in canonical correlation analysis graphically. For more than three sets of variables, we can apply the generalized canonical correlation biplot in generalized canonical correlation analysis which is an expansion of the canonical correlation analysis. On the other hand, we consider the set of covariate variables which is affecting the linearly two sets of variables. In this case, if we apply the generalized canonical correlation biplot, we cannot clearly interpret the other two sets of variables due to the effect of the set of covariate variables. Therefor, in this paper, we will apply the partial canonical correlation analysis of Rao (1969) removing the linear effect of the set of covariate variables on two sets of variables. We will suggest the partial canonical correlation biplot for inpreting the partial canonical correlation analysis graphically.

Development of Calibration System of Helium Permeation Type Standard Leaks (헬륨 투과형 표준리크 교정장치 개발)

  • Hong S.S.;Lim I.T.;Shin Y.H.
    • Journal of the Korean Vacuum Society
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    • v.15 no.4
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    • pp.347-353
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    • 2006
  • A helium permeation type standard leak calibration system has newly developed by using dynamic gas expansion method. The measurement range was extended lower to $10^{-6}$ Pa L/s for participating CCM (Consultative Committee for Mass and Related Quantities) standard leak key comparison. For the system, pressure ratios of high and ultra-high vacuum chamber and porous plug conductance for helium gas were determined. By using the system, a permeation type standard leak of $5.6{\times}10^{-4}$ Pa L/s range was calibrated. The calibration result showed that the difference between standard commercial leak was 11.1 %.

Area of Potential Arable Land Distributed on Hill side (개발가능지의 분포면적(分布面積))

  • Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.11 no.4
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    • pp.235-245
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    • 1979
  • The land utilization of Korea composed about 22.7% of arable land, 66.9% of forest land and 10.4% for the rest like a house site, road, and lake etc. The ratio of arable land is too small as compared with forest land which is occuping two-thirds of Korea. The expansion of arable land by the reclamation will solve the food problems for increasing populations. First, we have to know the exact extents and distribution of reclaimable land but the reported total extent of reclaimable land in Korea is variable according to the authority which carried out the soil survey. The different reported data of reclaimable land is owing to the method and criteria of soil survey. 840,522ha in Land Capability Survey of the Farm Land Improvement Association and 321,203ha in the Forest Land Survey by the Office of Forest was reported but the Office of Rural Development said that total reclaimable land was 1,400,540ha including grazing and fruit land. To assume the extent of reclaimable land, the soil survey method and criteria of soil survey should be considered. It can be considered that the main reasons of less in extent of reclaimable land in the other authorities than the Office of Rural Development is due to the following. The grazing land and the fruits land are not included by the Farm Land Improvement Association and only relative forest is considered by the Office of Forest. The main purpose of soil survey carried out by the Office of Rural Development is to utilize land of Korea at most. The soil characteristics and potential productivity are considered in soil survey and the reported data was based on reconnaissance soil survey. The more accurate data will be find out by the end of 1979 when the detailed soil survey will be done. In selection of cropping system and management, the soil condition should be considered, because the soil characteristics of reslaimed land is less fertile than the arable lands.

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Basic Properties of Waste Wood-Plastic Composite Panels by Hot Press Molding Method (열압 성형법에 의한 폐목재-플라스틱 복합패널의 기초적 성질)

  • Choi, Nak-Woon;Mun, Kyoung-Ju;Choi, San-Ho
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.4
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    • pp.95-104
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    • 2004
  • A styrene solution of waste expanded polystyrene with a crosslinking agent and an initiator was used as a binder for waste wood-plastic composite panels. The waste wood-plastic composite panels are prepared with various binder contents and filler-binder ratios by using a hot press molding method. The apparent density of the composite panels is increased with increasing binder content and filler-binder ratio, while their water absorption and expansion in thickness are decreased with increasing binder content and filler-binder ratio. The maximum flexural strength and wet flexural strength of the composite panels are obtained at a binder content of 35% and a filler-binder ratio of 0.8. Decreases in the flexural strengths of the composite panels due to water immersion at 20 and $100^{\circ}C$ are hardly recognized at binder contents of 30% or more.

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A study on expression methods for structural aesthetics in modern fashion design - Focus on the architectural characteristics of Santiago Calatrava - (현대 구조적 패션디자인에 나타난 구조미의 표현방식에 대한 연구 - 산티아고 칼라뜨라바의 건축특성을 중심으로 -)

  • Lee, Yeonji;Um, Sohee
    • The Research Journal of the Costume Culture
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    • v.23 no.5
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    • pp.737-754
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    • 2015
  • The structural aesthetics of architecture are becoming an inspirational source for many fashion designers and have been reborn in structural fashion. This study planned to analyze the method of expression of structural aesthetics expressed in modern structural fashion design and the construction method to maximize such an effect on the basis of the construction characteristic of Santiago Calatrava as the representative architect of the structural aesthetic. According to the study, the structural aesthetics expressed in modern structural fashion design are as follows: 1) The symbolical formative aesthetic expressed by symbolical inference and analyzation; 2) the dynamic beauty of physic expressed by visual emphasis and dynamics; and 3) the asymmetric beauty of symmetry expressed by metastasis toward the boundary between balance and imbalance. In addition, to maximize structural aesthetics, we used repetition and a progressive technique based on rhythm, asymmetry, and incision-based variances, such as balance, polygon flux, and inference, and analyzation-based distortion as the structuring principle. The following expression methods for maximizing structural aesthetics were found: 1) symbolical and structural exaggeration of appearance; 2) detail technique expansion and material property diversification; and 3) the three-dimensional transformation of structure and shell expression. Structural fashion design was found to have maximized structural aesthetics by using such expression methods to secure artistic esthetics, destroy existing shapes and patterns, and create unique shapes.

A Study on the Characteristics of Popular Traditional Expression in Small Commercial Buildings - Focus on a commercial buildings in a suburb of Taegu - (소규모(小規模) 상업(商業) 건축물(建築物)에 나타난 대중적(大衆的) 전통표현(傳統表現) 특성(特性)에 관(關)한 연구(硏究) - 대구시(大邱市) 인근지역(隣近地域) 상업건축물(商業建築物)을 사례(事例)로 -)

  • Rhee, Dong-Chan;Chang, Soo-Seok;Kim, Jung-Jae
    • Journal of architectural history
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    • v.12 no.4 s.36
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    • pp.63-79
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    • 2003
  • Considering the established research about the characteristics of traditional expression in a contemporary korean architecture, there are two critical problems. the one is that the extent of a case is limited to a public building and a large exhibition building. the other is that the angle of research focus on the viewpoint of specific a level. To settle these problems and supplement a established research, this study is to investigate and analyze the characteristics of popular traditional expression in small commercial buildings. In A viewpoint on the expansion of a case and a various angle, A small commercial buildings is selected as a case. because it is felt that the populace's taste vis-a-vis traditional expression. To investigate and analyze, A main analytic criterion is follows. 1) A expressional method on traditional architectural form 2) A expressional aspect by architectural element 3) A expressional aspect in architectural material 4) A expressional aspect in architectural structure. In consequence we can find out the following results in the process of analysis. 1) In expressional method on traditional architectural form, the traditional expression is represented as the imitation and transformation of a traditional folk house forms, and the embodiment of traditional image 2) In expressional aspect by architectural element, the traditional expression elements is mainly represented at a straw roof and a mud-plastered wall 3) the traditional expression material is mainly straw, timber, small wood boards and mud 4) In expressional aspect in architectural structure, the traditional expression is represented from the viewpoint of decorative expression.

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