• 생명과학회지
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• 제24권2호
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• pp.105-111
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• 2014

본 연구의 목적은 말의 열충격 단백질 유전자의 특성을 구명하고 말의 각 조직과 운동 전과 후 혈액에서 열충격 단백질 유전자의 발현량을 분석함에 있다. 이전의 연구를 통해, 대표적인 경주마인 더러브렛의 혈액과 골격근에서 운동 전, 후 RNA-sequencing을 통해 차등발현유전자 분석을 실시하고, 본 연구를 위해 운동 전과 후에 차등 발현된 유전자 중, 열충격 단백질 유전자(HspH1, Hsp90${\alpha}$, Hsp70)를 선택하였다. 세 개의 열충격 단백질 유전자는 각각의 혈액이나 근육에서 운동 전에 비해 후에 발현이 증가된 것으로 확인됐다. 본 연구팀은 선정된 유전자에 대한 검증과 분석을 위해, 말의 조직별 RT-PCR 분석과 운동시간별 백혈구에서 real time qPCR 분석을 실시하였다. 그 결과 말의 각 조직(갑상선, 결장, 골격근, 맹장, 신장, 심장, 척수, 폐)에서 세 개의 열충격 단백질 유전자 mRNA가 모두 존재함을 알 수 있었다. 또한, 말의 운동 시간 별 혈액에서 mRNA를 추출하여 열충격 단백질의 운동 시간에 따른 발현 양상 분석을 실시한 결과, 운동 전에 비해 운동 120분 후 열충격 단백질 유전자의 발현량이 증가함을 확인하였다. 이러한 결과는 인간과 다른 동물 실험의 결과와 일치하며, 열충격 단백질 유전자 전사 조절기작이 종간에 보존이 되어왔음을 시사한다. 또한, 운동에 따른 열충격 단백질 유전자의 발현 양상과 운동 수행 및 회복 기작간의 상관관계에 대한 추가적인 연구가 필요함을 제안하는 바이다.

• 한국IT서비스학회지
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• 제20권6호
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• pp.83-95
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• 2021

In this study, various usage patterns were analyzed after establishing a data mart for micro mobility analysis based on the rental history of public bicycles in Seoul. Rental history data is origin-destination data that includes the rental location and time, and the return location and time. About 1500 rental locations were classified according to the characteristics of the location to create a 'station type' dimension. We also created a 'path type' dimension that displays whether the rental location and return location are the same. In addition, a derived variable called speed, which is obtained by dividing the distance used by the time used, is added, and through this, the characteristics of the riding area and the reason for the rental can be estimated. Meanwhile, administrative district link, administrative neighborhood link, and station type link were created to apply network analysis. Through this analysis, the roles and proportions of administrative districts, public facilities, and private facilities engaged in micro-mobility services were visualized. 49.9% of rentals occur at rental offices near transportation facilities, and half of them occur at rental offices near subway stations. The number of rentals during the evening rush hour is more than double that of the morning rush hour. When the path type is unidirectional, there is a fixed destination, so the distance and time used are short, and the movement speed tends to be high. In the case of round-trip, the purpose of use is exercise or leisure, so the distance and time used are long, and the movement speed is slow. It is expected that the results of the analysis can be used as reference materials for selecting new rental locations, providing convenient services for users, and developing user-specialized products.

• 한국환경과학회지
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• 제31권3호
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• pp.219-226
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• 2022

This study was conducted to investigate the response of the human body to stress induced by wall recording of subway stations in the city center. The experiment was conducted as a simulation exercise, and six images were selected and produced based on Subway Line 2, a representative underground space in Seoul. The study participants included 24 male and female college students. A three-minute experiment was conducted, during which the participants were shown the control image and green wall image once each. To measure psychological status, the following measurement indicators were used: Semantic Differential, Positive Affect and Negative Affect Schedule and State-Trait Anxiety Inventory. Physiological changes were investigated by tracking participants' heart rate and blood pressure. Results showed that parasympathetic and sympathetic nerves were activated in the presence of the green wall in the subway station. The psychological evaluation analysis revealed that negative affect toward underground space decreased, while positive affect increased. This study found that the green wall in subway stations has a stable effect on the human body, both psychologically and physiologically. In the future, green walls in underground spaces can be used to reduce psychological stress and increase physiological relaxation.

• 콘크리트학회지
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• 제6권6호
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• pp.180-189
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• 1994

철근콘크리트 구조물의 손상을 진동반응특성치의 변화를 측정한 자료로부터 예측할 수 있는 실용적인 방법론이 제시되었다. 먼저, 구조물에 발생한 손상의 위치를 구조물 모드형상의 변화로부터 결정할 수 있는 알고리즘이 요약되었다. 다음으로, 실물크기 1/3 촉소 건조된 철근콘크리트 구조물을 사용한 실험에서 알고리즘을 이용하여 손상의 위치를 예측하였다. 이 실험과정에는 손상발생 전$\cdot$후의 소수의 진동반응특성치가 사용되었다. 구조물의 손상을 예측한 결과로부터 알고리즘이 손상을 정확하게 발견하는 것으로 판명되었다.

• Ocean and Polar Research
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• 제40권4호
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• pp.191-202
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• 2018

In this study we conducted a weekly monitoring exercise at a fixed station in the saltwater zone during the dry season (Jan-Mar, 2013) and wet season (Jun-Aug, 2013) to understand the fluctuations in phytoplankton communities and environmental factors in the Youngsan River estuary altered by a dike constructed in the coastal area. Phytoplankton communities displayed seasonality; diatoms were dominant during the dry season whereas dinoflagellates were dominant during the wet season. T-test analysis showed that water temperature was significantly different between the seasons whereas freshwater discharge from the dike was not significantly different. This suggests that seasonal variations of phytoplankton are more likely affected by water temperature than freshwater discharge. However, a short-term fluctuation was also observed in response to freshwater discharge; freshwater species appeared during or after the discharge in the dry and wet seasons and blooms of harmful species developed after the discharge. Phytoplankton communities may be affected by changes in physical factors such as turbidity and salinity and nutrient supply resulting from freshwater discharge. Especially, the nutrient supply may directly contribute to the harmful algal blooms (HABs) composed of dinoflagellates which can adapt to low salinity after freshwater discharge.

• Asian-Australasian Journal of Animal Sciences
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• 제28권6호
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• pp.870-875
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• 2015

The purpose of this study was to investigate the alternative splicing in equine cordon-bleu WH2 repeat protein-like 1 (COBLL1) gene that was identified in horse muscle and blood leukocytes, and to predict functional consequences of alternative splicing by bioinformatics analysis. In a previous study, RNA-seq analysis predicted the presence of alternative spliced isoforms of equine COBLL1, namely COBLL1a as a long form and COBLL1b as a short form. In this study, we validated two isoforms of COBLL1 transcripts in horse tissues by the real-time polymerase chain reaction, and cloned them for Sanger sequencing. The sequencing results showed that the alternative splicing occurs at exon 9. Prediction of protein structure of these isoforms revealed three putative phosphorylation sites at the amino acid sequences encoded in exon 9, which is deleted in COBLL1b. In expression analysis, it was found that COBLL1b was expressed ubiquitously and equivalently in all the analyzed tissues, whereas COBLL1a showed strong expression in kidney, spinal cord and lung, moderate expression in heart and skeletal muscle, and low expression in thyroid and colon. In muscle, both COBLL1a and COBLL1b expression decreased after exercise. It is assumed that the regulation of COBLL1 expression may be important for regulating glucose level or switching of energy source, possibly through an insulin signaling pathway, in muscle after exercise. Further study is warranted to reveal the functional importance of COBLL1 on athletic performance in race horses.

• 한국산학기술학회논문지
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• 제19권10호
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• pp.483-489
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• 2018

고령운전자의 운전행동은 신체적 및 인지적 기능의 저하로 돌발 상황이나 시내주행에서 안전운전에 취약하다고 보고되고 있다. 본 연구는 성인 자가운전자를 대상으로 연령대에 따라서 운전행동양상에 차이가 있는지 알아보고 정상적인 노화로 인하여 발생하는 운전에 관한 신체적 기능변화를 제언하기 위함이었다. 연구 참여자는 운전면허를 소지하고 실제로 자가운전을 하는 75명(20세-39세 21명, 40-64세 40명, 65세 14명)을 대상으로 하였다. 연구 참여자는 자기-기입 설문지를 통하여 성별, 우세 손, 약물복용 여부, 운동 여부, 연령 및 운전 기간을 응답하였다. 또한 조용한 운전능력 실험실에서 운전운동평가 기기를 통하여 조향, 가속 및 제어 등의 운전운동평가를 실시하였다. 연구결과, 파워검사는 연령대에 따라서 유의한 차이가 없었으나, 타이머검사 및 응급제어검사의 총 시간은 연령에 따라 유의미하게 차이가 있으며, 20세~39세에서 가장 빠르고, 65세 이상의 노인에서 가장 느리게 나타났다. 본 연구결과를 바탕으로 정상적인 노화는 고령 운전자의 운전행동양상과 연관된 지각-인지과정에 영향을 미친다고 제언하는 바이다.

• 지역사회간호학회지
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• 제28권4호
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• pp.524-536
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• 2017

Purpose: This study aims to examine the relationships among experiences of traumatic events, post-traumatic stress (PTS), and the needs for health promotion programs of 119 paramedics. Methods: Experiences of traumatic events, PTS, and needs for health promotion programs were measured using a structured questionnaire. The subjects were 193 paramedics in Jeju Island. Data were analyzed using descriptive statistics, t-test and $x^2$ test. Results: The most experienced event out of the 16 different traumatic events was 'retrieve a suicide's body.' A high-risk classification of PTS was observed in 36.2% of the subjects. The rates of experiences were higher than those of the actual needs for all 26 health promotion programs. The programs reported as highly needed by respondents were stress management (75.5%) followed by exercise (74.6%) and PTS management (72.5%). Subjects with longer career periods, a fire sergeant, perceived obesity, numerous experiences of traumatic events, and in the PTS risk group had a higher need for a stress management program. Conclusion: These findings indicate that a significant number of 119 paramedics experienced PTS. They also show that onsite strategic management is strongly required. In addition, implementation of health promotion programs based on the needs of 119 paramedics is highly needed.

• 한국약용작물학회지
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• 제24권1호
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• pp.1-6
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• 2016

Background : Irregular meals and insufficient exercise are major modern lifestyle-related risk factors for constipation. This study aimed to examine the effects of the aqueous extract of Dendrobium speciosum var. (DM) on the improvement of intestinal function and prevention of constipation in rats. Methods and Results : Constipation in rat was induced by loperamide (4 mg/kg) injection for 5 days and rats were randomly assigned to the following groups: normal control rats (NOR), constipated rats (LOP induced) and constipated rats supplemented with dulcolax-S (POS), DMSG, 100 mg/kg (DMSG-100), 300 mg/kg (DMSG-300) and 500 mg/kg (DMSG-500). The DMSG groups showed increments in the frequency and amount of stools compared to the LOP group. Loperamide treatment markedly reduced the water content of feces, whereas DMSG administration significantly increased fecal water contents. Moreover, DMSG improved intestinal transit speed in constipated-rats. Alcian blue staining revealed increased mucus production by crypt cells and mucus contents in feces and the mucosal surface. Conclusions : In summary, DM extraction significantly improves intestinal function in rats, which indicates the effectiveness of this extract in the prevention and treatment of constipation.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2006년도 춘계종합학술대회
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• pp.421-425
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• 2006

헬스케어와 무선 기술의 접목은 새로운 생체신호 모니터링 방법과 환자의 이동성 및 편의성을 제공하며 더 나은 방법으로 환자를 돌볼 수 있으며 이러한 장점으로 인해 최근 무선기술을 이용한 ECG 모니터링 및 계측 시스템이 개발되고 있다. 본 논문에서는 중요한 생체신호 중 가장 중요한 신호의 하나인 ECG 신호를 무선센서네트워크를 이용하여 무선으로 받은 후, 이를 서버컴퓨터에서 의사, 간호사 또는 환자의 보호자에게 진단의 기초자료로 제공할 수 있게 빈맥, 서맥, 동정지와 같은 비정상적인 ECG신호를 판단하는 ECG 모니터링 시스템을 구현하였다. 신체에서 계측된 ECG신호는 무선으로 서버와 RS-232로 연결된 베이스스테이션으로 전송되고 서버는 비정상적인 ECG 신호를 검사하여 저장 및 모니터링을 위해 PC/PDA로 데이터를 전송하며, 이러한 시스템을 활용하여 의료비 절감 및 더 편리한 의료서비스를 받을 수 있을 것으로 예상된다.