Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Dendrobium speciosum Var. Aqueous Extract on Loperamide-Induced Constipation in Rats

대명석곡추출물이 Loperamide로 유도된 변비에 미치는 효과

  • Jang, Ji Hun (National Development Institute of Korean Medicine) ;
  • Jung, Ho Kyung (National Development Institute of Korean Medicine) ;
  • Sim, Mi Ok (National Development Institute of Korean Medicine) ;
  • An, Byeong Kwan (National Development Institute of Korean Medicine) ;
  • Kim, Min Suk (National Development Institute of Korean Medicine) ;
  • Kang, Byoung Man (National Development Institute of Korean Medicine) ;
  • Cho, Jung Hee (National Development Institute of Korean Medicine) ;
  • Bean, Chul Gu (Research Institute, Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Kim, Seong Cheol (Namhae Sub-Station, NIHHS, RDA) ;
  • Jung, Won Seok (National Development Institute of Korean Medicine)
  • Received : 2015.11.18
  • Accepted : 2016.01.12
  • Published : 2016.02.28
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Abstract

Background : Irregular meals and insufficient exercise are major modern lifestyle-related risk factors for constipation. This study aimed to examine the effects of the aqueous extract of Dendrobium speciosum var. (DM) on the improvement of intestinal function and prevention of constipation in rats. Methods and Results : Constipation in rat was induced by loperamide (4 mg/kg) injection for 5 days and rats were randomly assigned to the following groups: normal control rats (NOR), constipated rats (LOP induced) and constipated rats supplemented with dulcolax-S (POS), DMSG, 100 mg/kg (DMSG-100), 300 mg/kg (DMSG-300) and 500 mg/kg (DMSG-500). The DMSG groups showed increments in the frequency and amount of stools compared to the LOP group. Loperamide treatment markedly reduced the water content of feces, whereas DMSG administration significantly increased fecal water contents. Moreover, DMSG improved intestinal transit speed in constipated-rats. Alcian blue staining revealed increased mucus production by crypt cells and mucus contents in feces and the mucosal surface. Conclusions : In summary, DM extraction significantly improves intestinal function in rats, which indicates the effectiveness of this extract in the prevention and treatment of constipation.

Keywords

References

  1. Aichbichler BW, Wenzel HH, Santa Ana CA, Porter JL, Schiller LR and Fordtran JS. (1998). A comparison of stool characteristic from normal and constipated people. Digestive Diseases and Sciences. 43:2353-2362. https://doi.org/10.1023/A:1026699525487
  2. Bongers MEJ, Benninga MA, Maurice-Stam H and Grootenhuis MA. (2009). Health-related quality of life in young adults with symptoms of constipation continuing from childhood into adulthood. Health and Quality of Life Outcomes. 7:20 paragraphs. http://hqlo.biomedcentral.com/articles/10.1186/1477-7525-7-20 (cited by 2015 Sep 14).
  3. Cepinskas G, Specian RD and Kvietys PR. (1993). Adaptive cytoprotection in the small intestine: Role of mucus. American Journal of Physiology. 264:G921-G927.
  4. Choi JS, Kim JW, Cho HR, Kim KY, Lee JK, Sohn JH and Ku SK. (2014). Laxative effects of fermented rice extract in rats with loperamide-induced constipation. Experimental and Therapeutic Medicine. 8:1847-1854. https://doi.org/10.3892/etm.2014.2030
  5. Holzer P. (2009). Opioid receptors in the gastrointestinal tract. Regulatory Peptides. 155:11-17. https://doi.org/10.1016/j.regpep.2009.03.012
  6. Hughes S, Higgs NB and Turnberg LA. (1984). Loperamide has antisecretory activity in the human jejunum in vivo. Gut. 25:931-935. https://doi.org/10.1136/gut.25.9.931
  7. Ito M, Matsuzaki K, Wang J, Daikonya A, Wang NL, Yao XS and Kitanaka S. (2010). New phenanthrenes and stilbenes from Dendrobium loddigesii. Chemical and Pharmaceutical Bulletin. 58:628-633. https://doi.org/10.1248/cpb.58.628
  8. Jun DW, Park HY, Lee OY, Lee HL, Yoon BC, Choi HS, Hahm JS, Lee MH, Lee DH and Kee CS. (2006). A population-based study on bowel habits in a Korean community: Prevalence of functional constipation and self-reported constipation. Digestive Diseases and Sciences. 51:1471-1477. https://doi.org/10.1007/s10620-006-9087-3
  9. Jung HK, Jang JH, Ko JH, Kang BM, Yeo JH, Cho JH, Cho HW, Bean CG, Kim SC and Jung WS. (2014). Whitening and anti-wrinkle effects of Korean native Dendrobium moniliforme methanol extract. Korean Journal of Medicinal Crop Science. 22:331-338. https://doi.org/10.7783/KJMCS.2014.22.5.331
  10. Kakino M, Tazawa S, Maruyama H, Tsuruma K, Araki Y, Shimazawa M and Hara H. (2010). Laxative effects of agarwood on low-fiber diet-induced constipation in rats. BMC Complementary and Alternative Medicine. 10:68. http://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-10-68(cited by 2015 Sep 14).
  11. Kim DG, Jin YG, Jin JY, Kim SC, Kim SC, Han CH and Lee YJ. (2011). Effects of the Actindia chinensis on loperamide-induced constipation in rat. Korean Journal of Plant Resources. 24:61-68. https://doi.org/10.7732/kjpr.2011.24.1.061
  12. Kim YG, Yang GH and Cho SI. (2005). Anti-oxidative effects of Dendrobii herba on toxic agent induced kidney cell injury. Korea Journal of Herbology. 20:53-60.
  13. Lembo A and Camilleri M. (2003). Chronic constipation. New England Journal of Medicine. 349:1360-1368. https://doi.org/10.1056/NEJMra020995
  14. Li Y, Wang CL, Wang YJ, Wang FF, Guo SX, Yang JS and Xiao PG. (2009). Four new bibenzyl derivatives from Dendrobium candidum. Chemical and Pharmaceutical Bulletin(Tokyo). 57:997-999. https://doi.org/10.1248/cpb.57.997
  15. Mertz H, Naliboff B and Mayer E. (1999). Physiology of refractory chronic constipation. The American Journal of Gastroenterology. 94:609-615. https://doi.org/10.1111/j.1572-0241.1999.922_a.x
  16. Miyazawa M, Shimamura H, Nakamura S and Kameoka H. (1997). Antimutagenic activity of gigantol from Dendrobium nobile. Journal of Agricultural and Food Chemistry. 45:2849-2853. https://doi.org/10.1021/jf9603902
  17. Moretti M, Cossignani L, Messina F, Dominici L, Villarini M, Curini M and Marcotullio MC. (2013). Antigenotoxic effect, composition and antioxidant activity of Dendrobium speciosum. Food Chemistry. 140:660-665. https://doi.org/10.1016/j.foodchem.2012.10.022
  18. Park MH, Kwon CJ, Lim SH, Kim KH, Heo NK, Jang HK, Park IJ and Lee KJ. (2011). Effects of dietary fiber isolated from Synurus deltoides on constipation in loperamide-induced rats. Journal of the Korean Society of Food Science and Nutrition. 40:1715-1719. https://doi.org/10.3746/jkfn.2011.40.12.1715
  19. Rao SSC. (2001). Dyssynergic defecation. Gastroenterology Clinics of North America. 30:97-114. https://doi.org/10.1016/S0889-8553(05)70169-2
  20. Shimotoyodome A, Meguro S, Hase T, Tokimitsu I and Sakata T. (2000). Decreased colonic mucus in rats with loperamide-induced constipation. Comparative Biochemistry and Physiology. 126:203-211. https://doi.org/10.1016/S1095-6433(00)00194-X
  21. Sohji Y, Kawashima K and Shimizu M. (1978). Pharmacological studies of loperamide, an anti-diarrheal agent. II. Effects on peristalsis of the small intestine and colon in guinea pigs. Folia Pharmacologica Japonica. 74:155-163. https://doi.org/10.1254/fpj.74.155
  22. Suzuki M, Hayakawa Y, Aoki K, Nagase H, Nakamura H, Yamada K and Hirata Y. (1973). Stereochemistry of intermediates in the syntheses of Dendrobium alkaloids. Tetrahedron Letters. 14:331-334. https://doi.org/10.1016/S0040-4039(01)95654-0
  23. Watanabe T, Nakaya N, Kurashima K, Kuriyama S, Tsubono Y and Tsuji I. (2004). Constipation, laxative use and risk of colorectal cancer: The Miyagi cohort study. European Jouranl of Cancer. 40:2109-2115. https://doi.org/10.1016/j.ejca.2004.06.014
  24. Yang H, Chou GX, Wang ZT, Guo YW, Hu ZB and Xu LS. (2004). Two new compounds from Dendrobium chrysotoxum. Helvetica Chimica Acta. 87:394-399. https://doi.org/10.1002/hlca.200490037
  25. Zhang H, Liao W, Chao W, Chen Q, Zeng H, Wu C, Wu S and Ho HI. (2008). Risk factors for sebaceous gland diseases and their relationship to gastrointestinal dysfunction in Han adolescents. The Journal of Dermatology. 35:555-561. https://doi.org/10.1111/j.1346-8138.2008.00523.x
  26. Zhao C, Liu Q, Halaweish F, Shao B, Ye Y and Zhao W. (2003). Copacamphane, picrotoxane and alloaromadendrane sesquiterpene glycosides and phenolic glycosides from Dendrobium moniliforme. Journal of Natural Products. 66:1140-1143. https://doi.org/10.1021/np0301801