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Development of line-scanning two-photon microscopy based on spatial and temporal focusing for tryptophan based auto fluorescence imaging (고속 트립토판 자가형광 이미징을 위한 시공간적 집중 기반의 라인 스캐닝 이광자 현미경 개발)

  • Lee, Jun Ho;Nam, Hyo Seok;Kim, Ki Hean
    • Journal of the Korean Society of Visualization
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    • v.11 no.2
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    • pp.41-45
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    • 2013
  • Two-photon microscopy (TPM) is minimally-invasive 3D fluorescence microscopy based on nonlinear excitation, and TPM can visualize cellular structures based on auto-fluorescence. Line-scanning TPM is one of high-speed TPM methods without sacrificing the image resolution by using spatial and temporal focusing. In this paper, we developed line-scanning TPM based on spatial and temporal focusing for auto-fluorescence imaging by exciting the tryptophan. Laser source for this system was an optical parametric oscillator (OPO) and it made near 570 nm femtosecond pulse laser. It had 200fs pulse width and 1.72 nm bandwidth, so that the achievable depth resolution was 2.41um and field of view (FOV) is 10.8um. From the characterization, our system has 3.0 um depth resolution and 12.3 um FOV. We visualized fixed leukocyte cell sample and compared with point scanning system.

A Study on TSIUVC Approximate-Synthesis Method using Least Mean Square (최소 자승법을 이용한 TSIUVC 근사합성법에 관한 연구)

  • Lee, See-Woo
    • The KIPS Transactions:PartB
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    • v.9B no.2
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    • pp.223-230
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    • 2002
  • In a speech coding system using excitation source of voiced and unvoiced, it would be involves a distortion of speech waveform in case coexist with a voiced and an unvoiced consonants in a frame. This paper present a new method of TSIUVC (Transition Segment Including Unvoiced Consonant) approximate-synthesis by using Least Mean Square. The TSIUVC extraction is based on a zero crossing rate and IPP (Individual Pitch Pulses) extraction algorithm using residual signal of FIR-STREAK Digital Filter. As a result, This method obtain a high Quality approximation-synthesis waveform by using Least Mean Square. The important thing is that the frequency signals in a maximum error signal can be made with low distortion approximation-synthesis waveform. This method has the capability of being applied to a new speech coding of Voiced/Silence/TSIUVC, speech analysis and speech synthesis.

Time-Varying Seismogenic Coulomb Electric Fields as a Probable Source for Pre-Earthquake Variation in the Ionospheric F2-Layer

  • Kim, Vitaly P.;Hegai, Valery V.;Liu, Jann Yenq;Ryu, Kwangsun;Chung, Jong-Kyun
    • Journal of Astronomy and Space Sciences
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    • v.34 no.4
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    • pp.251-256
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    • 2017
  • The electric coupling between the lithosphere and the ionosphere is examined. The electric field is considered as a timevarying irregular vertical Coulomb field presumably produced on the Earth's surface before an earthquake within its epicentral zone by some micro-processes in the lithosphere. It is shown that the Fourier component of this electric field with a frequency of 500 Hz and a horizontal scale-size of 100 km produces in the nighttime ionosphere of high and middle latitudes a transverse electric field with a magnitude of ~20 mV/m if the peak value of the amplitude of this Fourier component is just 30 V/m. The time-varying vertical Coulomb field with a frequency of 500 Hz penetrates from the ground into the ionosphere by a factor of ${\sim}7{\times}10^5$ more efficient than a time independent vertical electrostatic field of the same scale size. The transverse electric field with amplitude of 20 mV/m will cause perturbations in the nighttime F region electron density through heating the F region plasma resulting in a reduction of the downward plasma flux from the protonosphere and an excitation of acoustic gravity waves.

Sustainable Vibration Energy Harvesting Based on Zr-Doped PMN-PT Piezoelectric Single Crystal Cantilevers

  • Moon, Seung-Eon;Lee, Sung-Q;Lee, Sang-Kyun;Lee, Young-Gi;Yang, Yil-Suk;Park, Kang-Ho;Kim, Jong-Dae
    • ETRI Journal
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    • v.31 no.6
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    • pp.688-694
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    • 2009
  • In this paper, we present the results of a preliminary study on the piezoelectric energy harvesting performance of a Zr-doped $PbMg_{1/3}Nb_{2/3}O_3-PbTiO_3$ (PMN-PZT) single crystal beam. A novel piezoelectric beam cantilever structure is used to demonstrate the feasibility of generating AC voltage during a state of vibration. The energy-harvesting capability of a PMN-PZT beam is calculated and tested. The frequency response of the cantilever device shows that the first mode resonance frequency of the excitation model exists in the neighborhood of several hundreds of hertz, which is similar to the calculated value. These tests show that several significantly open AC voltages and sub-mW power are achieved. To test the possibility of a small scale power source for a ubiquitous sensor network service, energy conversion and the testing of storage experiment are also carried out.

The computation of the torso surface potentials using the boundary element method (경계요소법을 이용한 트로소 표면전위의 계산)

  • 이경중;이세진
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.33B no.8
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    • pp.22-29
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    • 1996
  • This study describes a method to find the torso surface potential based on the boundary element method. In order to find the torso surface potential, the governing equation was developed based on the green's second theorem. The boundary element method (BEM) which has a good computing capability in case of homogeneous and isotropic medium was applied to solve the equation. to validate the BEM, we considered a homogeneous sphere model which has an electrric dopole source inside. The results showed the good agreement between the analytic solution and the computed solution. In normal heart, the simulated torso surface isopotential maps are good agreement with that obtained form the ventricular excitation.

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Preparation and Luminescene properties with invisible inoranic phosphors of nano size (나노크기의 비가시 무기형광체 제조와 발광특성)

  • Jeong, Jae-Hoon;Yun, Hyun;Jang, Gyu-Hwan;Shin, Sang-Sik
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.461-462
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    • 2008
  • $BaMgAl_{10}O_{17}$:Mn, $Eu^{2+}$ green phosphors has been synthesized by the solid state reaction. Green phosphors of nano-size were manufactured in short time by shake method. which were easily manufactured respectively general method. Green phosphors of nano size were control additive, size of $ZrO_3$ ball, shake time and weight of ball in toluene. In result that green phosphors were obtained particle size of 140nm~150nm. The characteristics of fired samples were obtained by 365nm and 380nm excitation source under ultraviolet. In result that the highest PL intensity were observed in wavelength of 365nm.

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An analysis of crosstalk in hihg-speed packaging interconnects using the finite difference time domain method (시간 영역 유한 차분법을 이용한 고속 패키지 접속 선로의 누화 해석)

  • 남상식;장상건;진연강
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.22 no.9
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    • pp.1975-1984
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    • 1997
  • In this paper, we analyzed the frequency characteristics and the crosstalk of the adjacent parallel lines and the crossed lines in high-speed packaging interconnections by using the three-dimensional finite difference time domain (3D FDTD) method. To analyze the actual crosstalk phenomena in the transmission of the high-speed digital sgnal, the step pulse with fast rise time was used for the source excitation signal instead of using the Gaussian pulse that is generally used in FDTD. To veify the theoretical resutls, the experimental interconnection lines that were fabricated on the Duroid substrate($\varepsilon_{r}$=2.33, h=0.787 [mm]) were tested by TDR(time domain reflectometry). The results show good agreement between the analyzed results and the tested outcomes.

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Aerosol Particle Analysis Using Microwave Plasma Torch (마이크로파 플라즈마 토치를 이용한 에어로졸 입자 분석)

  • Kim, Hahk-Joon;Park, Ji-Ho
    • Journal of the Korean Chemical Society
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    • v.55 no.2
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    • pp.204-207
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    • 2011
  • A particle counting system that can also provide sensitive, specific chemical information, while consuming very less power, occupying less space, and being inexpensive has been developed. This system uses a microwave plasma torch (MPT) as the excitation source for atomic emission spectrometry (AES). Emission from a single particle can be detected, and the wavelength at which the emission is observed indicates the elements present in the particle. It is believed that correlating the particle size and emission intensity will allow us to estimate the particle size in addition to abovementioned capabilities of the system. In the long term, this system can be made field-portable, so that it can be used in atmospheric aerosol monitoring applications, which require real-time detection and characterization of particles at low concentrations.

The Biologic Effect of Millimeter Wave Irradiation Followed to Photodynamic Therapy on the Tumor

  • Ahn, Jin-Chul;Lee, Chang-Sook;Chang, So-Young;Yoon, Sung-Chul
    • Biomedical Science Letters
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    • v.17 no.1
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    • pp.79-84
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    • 2011
  • Photodynamic therapy consists of a photosensitizer, suitable light source and oxygen. The excitation of the photosensitizer at a cancer mass results in oxidation which would ultimately reduce the mass via apoptosis. Millimeter wave (MMW) therapy has also been known to be effective on cancer cell mass reduction, human cell regeneration and immunity enhancement among the Russian clinicians and scientists. In the present study, the two modalities were combined to achieve synergistic effects while reducing the administration dosage of the photosensitizer, photogem, thus minimizing the side effects. The CT-26 adenocarcinoma cell mass was implanted on mice and the tumors were exposed to a simple MMW irradiation or a combined treatment of MMW and PDT. The treatments continued for 4 weeks and the size of the tumor was measured continuously. The significant therapeutic result of MMW was not found during 4 weeks, preferably more cancer recurrence possibility after MMW irradiation was observed. The results of this study suggest that the combination of MMW irradiation and photodynamic treatment should not be recommended. The result of the MMW treatment alone, however, displayed suppressive effect on cancer cell proliferation for both in vitro and in vivo. The results of the present study suggest that the millimeter wave therapy deserves a further study.

Magnetic Measurement of PLS Storage Ring Prototype Dipole Magnet (방사광 가속기용 2극 전자석 특성측정 및 보정)

  • 강봉구;박기현;윤종렬;구양모
    • Journal of the Korean Magnetics Society
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    • v.2 no.2
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    • pp.163-168
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    • 1992
  • Results of magnetic measurement of a prototype Storage Ring dipole magnet for the Pohang Light Source (PLS) are summarized. Hall probe mapping and rotating coil methods are used to measure field profiles and integrated field properties. End chamfering experiments are performed on the as-built magnet, which has four removable pole-end pieces, to meet the specifications set from beam dy-namics requirement. The corrected magnet has efficiency of 99.7% at 2.0 GeV excitation ad higher order multipole error levels less than the specifications for all excitations, showing that the magnet has appropriate properties for the PLS Storage Ring.

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