• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.4
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• pp.1-15
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• 2018

Objectives: Osteoporosis is considered a serious human disease. We developed an extract of mixed herbs containing root of Platycodon grandiflorum (ExMH-PGR), which is expected to be effective in preventing or treating osteoporosis. The aim of this study was to investigate the anti-osteoporotic effect of ExMH-PGR in osteoblastic MC3T3-E1 cells and osteoclastic RAW 264.7 cells. Methods: To examine the anti-osteoporotic effect of ExMH-PGR, osteoblast and osteoclast differentiation were induced and cultured with various concentrations of ExMH-PGR. Alkaline phosphatase (ALP) activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells were analyzed. Tartrate-resistant acid phosphatase (TRAP) activity and the formation of actin ring in RAW 264.7 cells were analyzed. Results: ExMH-PGR at concentration up to $25{\mu}g/mL$ significantly increased ALP activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells. ExMH-PGR at 50 to $200{\mu}g/mL$ significantly inhibited TRAP activity and the formation of actin ring in RAW 264.7 cells. Conclusions: These results demonstrate that ExMH-PGR stimulates osteoblastic activities and inhibits osteoclastic activities in in vitro systems, suggesting that ExMH-PGR might be considered as an anti-osteoporotic candidate for treatment of osteoporosis disease.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.110-117
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• 2007

In vitro toxicity tests such as cytotoxicity, mutagenicity and genotoxicity assay are useful for evaluating the relative toxicity of smoke or smoke condensates obtained from different cigarette configurations. A major disadvantage of these tests is relatively time-consuming, complicated and expensive. Recently, a cell-free glutathione consumption assay (GCA) as a rapid and simple screening method for the toxicity assessment of smoke has been reported by Cahours et al. (CORESTA, 2006). This study was carried out to assess the GCA application capable of predicting the toxicity of gas/vapor phase (GVP) of cigarette smoke and to identify individual compounds responsible for the glutathione (GSH) consumption in smoke. Each GVPs from 2R4F, standard cigarette, carbon filter cigarette (ExC) and new carbon filter cigarette (ExN), test cigarettes were collected by automatic smoking machine and evaluated the relative toxicity by GCA and neutral red uptake (NRU) assay. Toxic compounds existed in smoke were also chosen, relative toxicities of these compounds were screened by using two methods and compared individually. The overall order of toxicity by GCA was 2R4F > ExC > ExN, which was consistent with the result of Neutral Red Uptake assay. The levels of carbonyl compounds of ExN were lower than those of 2R4F and ExC, indicating that GSH consumption was associated with carbonyl compound yields. A major toxicant under current study is acrolein, which contributed to more than half of the GSH consumption. Collectively, the toxicity of GVP determined by GCA method may be mainly attributed to acrolein.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.2
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• pp.173-178
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• 1984

Correlation study was conducted to establish the optimum test level of soil improvement for good growth of corn from the relationships between the corn yield and soil chemical properties in 16 farmers' demonstration fields. Significant positive correlations between the corn yield and soil chemical properties ; pH, available phosphorus, exchangeable potassium, calcium, magnesium, cation exchange capacity. available silica, and base saturation percentage were showed but organic matter and nitrogen content were not. The proper nutrient contents in soil for expecting corn yield, 1.000kg/10a could be estimated as pH 5.6, available phosphorus 327ppm, exchangeable potassium 0.39me/100g, exchangeable calcium 5.5me/100g, exchangeable magnesium 1.3me/100g, cation exchange capacity 11.5me/100g, available silica 116ppm, base saturation percentage 58 from the relationships between the corn yield and soil chemical properties. Exchangeable aluminium were negatively correlated with not only corn yield but also pH, available phosphorus, exchangeable potassium and exchangeable calcium.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.38 no.6
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• pp.671-679
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• 2020

In order to increase the food self-sufficiency rate, monitoring and analysis of crop conditions in the cultivated area is important, and the existing measurement methods in which agricultural personnel perform measurement and sampling analysis in the field are time-consuming and labor-intensive for this reason inefficient. In order to overcome this limitation, it is necessary to develop an efficient method for monitoring crop information in a small area where many exist. In this study, RGB images acquired from unmanned aerial vehicles and vegetation index calculated using RGB image were applied as deep learning input data to classify complex upland crops in small farmland. As a result of each input data classification, the classification using RGB images showed an overall accuracy of 80.23% and a Kappa coefficient of 0.65, In the case of using the RGB image and vegetation index, the additional data of 3 vegetation indices (ExG, ExR, VDVI) were total accuracy 89.51%, Kappa coefficient was 0.80, and 6 vegetation indices (ExG, ExR, VDVI, RGRI, NRGDI, ExGR) showed 90.35% and Kappa coefficient of 0.82. As a result, the accuracy of the data to which the vegetation index was added was relatively high compared to the method using only RGB images, and the data to which the vegetation index was added showed a significant improvement in accuracy in classifying complex crops.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.16 no.7
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• pp.604-610
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• 2003

The effects of sintering additives such as B $i_2$ $O_3$ and $V_2$ $O_{5}$ on the microwave dielectric and sintering properties of (Z $n_1$$_{-xM}$ $g_{x}$)Ti $O_3$ system were investigated. Highly dense samples were obtained for (Z $n_{0.8}$M $g_{0.2}$)Ti $O_3$ at the sintering temperature range of 870~90$0^{\circ}C$ with B $i_2$ $O_3$ and $V_2$ $O_{5}$ additions of 〈1 wt.%, respectively. The microwave dielectric properties of (Z $n_{0.8}$M $g_{0.2}$)Ti $O_3$ with 0.45 wt.%B $i_2$ $O_3$ and 0.55 wt.% $V_2$ $O_{5}$ sintered at 90$0^{\circ}C$ were as follows : Q$\times$ $f_{o}$ = 50,800 GHz, $\varepsilon$$_{r}$ = 22, and $\tau$$_{f}$ = -53 ppm/$^{\circ}C$. In order to improve temperature coefficient of resonant frequency, Ti $O_2$ was added to the above system. The optimum amount of Ti $O_2$ was 15 moi.% when sintered at 87$0^{\circ}C$, at which we could obtain following results: Q$\times$ $f_{o}$ = 32,800 GHz, $\varepsilon$$_{r}$ = 26, and$\tau$$_{f}$ = 0 ppm/$^{\circ}C$.EX>.EX>.EX>.EX>.EX>.EX>.EX>.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.37 no.5
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• pp.351-357
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• 2019

UAV (Unmanned Aerial Vehicle) images can be exploited for rapid forest fire damage assessment by virtue of UAV systems' advantages. In 2019, catastrophic forest fire occurred in Goseong and Sokcho, Korea and burned 1,757 hectares of forests. We visited the town in Goseong where suffered the most severe damage and conducted UAV flights for forest fire damage assessment. In this study, economic and rapid damage assessment method for forest fire has been proposed using UAV systems equipped with only a RGB sensor. First, forest masking was performed using automatic elevation thresholding to extract forest area. Then ExG (Excess Green) vegetation index which can be calculated without near-infrared band was adopted to extract damaged forests. In addition, entropy filtering was applied to ExG for better differentiation between damaged and non-damaged forest. We could confirm that the proposed forest masking can screen out non-forest land covers such as bare soil, agriculture lands, and artificial objects. In addition, entropy filtering enhanced the ExG homogeneity difference between damaged and non-damaged forests. The automatically detected damaged forests of the proposed method showed high accuracy of 87%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.1
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• pp.13-20
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• 1991

For this experiment frist the fermented Soybean Meju was prepared by domestic ordinary method of Korean and concentrated Meju suspensoidal extract was made for toxicological test sample on mice and rat. Even the oral dose of 10g extract per kg of mice had not any acute toxicity. So the median lethal dose(LOD50) is lilely to be much greatr or not to be. In comparison of the average weight gain rate for 4 weeks the control and test groups of 0.308g Ex/rat kg and 9.24g Ex/rat kg daily were the much the same as about 19% but 3.08g Ex/rat kg group waas exceptionally 2.7% higher than control For this period all test groups did not show any abnormality in appearance and anatomic findings. In serum GPT GOT alkaline phosphatase and blood urea nitrogen value there were no difference significantly between control and the test groups of 0.308g/rat kg and 3.08g/rat kg but 9.24g/rat kg group was some what higher than control. Especially in cholesterol alkaline phosphatase and blood urea nitrogen value 9.24g/rat kg group or 28days had 100mg/이 37.5u 32mg/이 respectively which were increased significantly. The pathological findings of all test groups especially 9.24g/kg group did not showed any characterized sign of acute or subacute liver damage or intestine lesion. So it is supposed that there is not any tocixants originated from some molds in fermented Korean domestic Meju which is prepared traditionally in winter.

• Natural Product Sciences
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• v.15 no.4
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• pp.185-191
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• 2009

Intestinal epithelial cells (IEC) play an important role in the mucosal immune system. IEC-derived mediators of inflammatory cascades play a principal role in the development of colon inflammation. The aim of this study was to investigate the inhibitory effect of aqueous extracts of Schizandra chinensis fruits (SC-Ex) on the production of inflammatory mediators by the human colonic epithelial cells. HT-29 cells were stimulated with dextran sulfate sodium in the presence or absence of SC-Ex to examine the cytoprotection and production of IL-8 and reactive oxygen species (ROS). It was shown that dextran sulfate sodium (DSS) caused the reduction of cell viability and production of IL-8 and ROS in DSS-treated HT-29 cells. We observed that the treatment of SC-Ex protected significantly cell proliferation from DSS-induced damage in dose-dependent manner. SC-Ex (10 and 100 ${\mu}g$/ml) also suppressed DSS-induced production of IL-8 mRNA and protein. Moreover, DSS-induced ROS production was inhibited markedly by the treatment of 100 ${\mu}g$/ml SC-Ex. These results suggest that SC-Ex has the protective effects on DSS-induced cell damage and the release of inflammatory mediators in the intestinal epithelial cells.

• Korean Journal of Plant Taxonomy
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• v.39 no.3
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• pp.216-219
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• 2009

The somatic chromosome of two taxa, Galium elegans Wall. ex Roxb(Sect. Cymogaliea Pobed) and Galium asperifolium Wall. ex Roxb(Sect. Leptogalium Lang), in Yunnan, China were investigated. The taxa were reported for the first time. The somatic chromosome numbers of G. elegans was 2n = 22(X = 11), diploid, from two regions, Mt. Canghsan and Hutiaoxia Valley. Those of G. asperifolium were found as 2n = 33, 44, 55(X = 11) with triploid, tetraploid, pentaploid. Most of G. elegans in the Yunnan were confirmed as diploid. The somatic chromosome number of G. asperifolium was found polyploidy, and the investigation revealed that triploid and tetraploid are living together as mixed population in the Mt. Canghsan.

• Preventive Nutrition and Food Science
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• v.15 no.3
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• pp.189-195
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• 2010

Water extracts from 20 medicinal plants, traditionally used for postmenopausal symptoms in Korea, were examined for their vasorelaxant activity in isolated rat thoracic aorta rings precontracted with norepinephrine (NE). Among the 20 medicinal plants, Cornus officinalis (CoEx, 0.3 mg/mL), Schisandra chinensis (ScEx, 0.3 mg/mL), Erythrina variegate (EvEx, 0.3 mg/mL), and Epimedium koreanum (EkEx, 0.3 mg/mL) showed rapid relaxation of endothelium-intact aorta ($69\pm4%$, $40\pm3%$, $25\pm2%$, and $23\pm3%$ of active tone induced by NE, respectively). In contrast, the extracts of Erythrina variegata (EvEx), Angelica gigas (AgEx), Pueraria thunbergiana (PtEx), and EkEx lead to gradual (i.e., long-term) relaxation to baseline in endothelium-intact vessels. The time to complete relaxation was 20～40 min. These 6 plant extracts were selected for the investigation of possible underlying mechanisms. The CoEx-, ScEx-, or EkEx-induced rapid relaxations were virtually abolished by endothelium denudation, and were significantly inhibited by pretreatment with nitric oxide (NO) synthase inhibitor $N^G$-nitro-L-arginine (L-NNA, 10 ${\mu}M$), indicating that increased formation of NO might contribute to the endothelium-mediated relaxation. In long-term responses, the endothelium denudation did not affect PtEx-induced relaxation, whereas it delayed responses by EvEx and AgEx, and significantly inhibited the effect of EkEx. Among EvEx, AgEx, and PtEx, EvEx attenuated the $CaCl_2$-induced vasoconstriction in high-potassium depolarized medium, implying that EvEx is involved in inhibition of the extracellular calcium influx to smooth muscle through voltage dependent calcium channels. These results provide the scientific rationale for the interrelationships between the use of 20 medicinal plants and their effects on cardiovascular health in estrogen deficient conditions.