• Journal of Food Hygiene and Safety
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• v.7 no.2
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• pp.83-89
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• 1992

This study was perfonned to investigate effectiveness eness on the gastritis and gastric lesion with the methanol extract of the flower buds of Eugenio caryophyllata. The extract was fractionated with hexane, chIorofonn, ethyl acetate, butanol, followed by bioassay Oil antigastritis. The ethyl acetate and the buthanol fraction reduced significantly HCI.ethanol induced gastric lesion at the dose of 165 and 215 mg/kg, p.o., respectively. These results may indicate that remarkably.effective are ethyl acetate and butanol fractions in HCI-ethanol induced gastric lesion. Howeever, the fractions didn't exhibit any inhibition of gastric secretion and acid output. The buthanol fraction reduced significantly the acetic acid induced ulcer at a daily dose of 215 mg/Kg, p.o., given for 10 days. These result showed considerable inhibit of acetic acid induced ulcer without inhibition of indomethacin induced gastric lesion. The methanol extract showed low acute toxicity with minimum lethal dose of more than 3000 mg/kg, p.o. in mice. In conclusion, Eugenia F10s exhibited antigastric activity which might be attributable to inhibition of gastric secretion. It is indicated that activie component may be present in the buthanol fraction.

• Journal of Applied Biological Chemistry
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• v.44 no.1
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• pp.23-26
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• 2001

The insecticidal activities of 30 aromatic plant extracts against four insect pests were examined by direct contact application. Against Nilaparvata lugens female adults, Myzus persicae female adults, and Plutella xylostella larvae, at 5,000 ppm, >90% mortality was achieved in the methanol extracts of the rhizomes from Cnidium officinale, Acorus gramineus, Acorus calamus var. angustatus and Curcuma Ionga, the whole plant from Agastache rugosa, the fruits from Illicium verum and Piper nigrum, and the flower bud from Eugenia caryophyllata as well as Cinnamomum camphora steam distillate. Against Spodoptera litura larvae, all test plant extracts were ineffective. The plants described merit further study as potential insect-control agents against insect pests.

• Korean Journal of Pharmacognosy
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• v.33 no.4 s.131
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• pp.319-323
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• 2002

This study was carried out to develop anticariogenic agents from natural products, the methanol and ethyl acetate extracts of 105 medicinal plants were tested for the antimicrobial activity against a cariogenic bacterium, Streptococcus mutans OMZ 176. Among them, the Aristolochia contorta, Dryopteris crassirhizoma, Eugenia caryophyllata, Hemerocallis fulva, Pinellia ternata, Pyrola japonica, and Scrophularia buergeriana showed significant antimicrobial activity.

• Journal of environmental and Sanitary engineering
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• v.17 no.3
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• pp.110-114
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• 2002

The repellent activity of methanol extracts from 8 aromatic medicinal plant species and a steam distillate against blood-starved Aedes aegypti (L.) females was laboratory examined by skin test and compared with that of N,N-diethyl-m-toluamide (deet). Reponeses varied according to Plant species. At a dose of $0.1mg/\textrm{cm}^2$, potent repellency against adult mosquitoes was obtained with extracts of Cinnamomum camphora (94.1 %) Cinnamomum cassia bark (91.2%), Eugenia caryophyllata flower bud (72.2%), and Tilia amurensis (69.4%). Repellent activity of these plant extracts was comparable to that of deet (83.4%). The plants described merit further study as potential mosquito-repellent agents.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.2
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• pp.115-123
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• 1996

Methanolic aqueous extracts of 100 plants were screened for antioxidative activity using Fenton's reagen/ethyl linoeate system and free radical scavenging activity using DPPH free radical generating system. The results suggest that at least six plants including Eugenia caryophyllata, Alpinia offiicinarum, Rhus verniciflua, Curcuma longa, Rheum palmatvm and Evodia officinalis may be the potential sources of antioxidant, But only one plant, Cornus officinalis, may be the potential source of free radical scavenger from natural plants.

• The Korean Journal of Food And Nutrition
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• v.16 no.4
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• pp.328-333
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• 2003

When the extract of the medicinal plants, Kaempferia galanga L., Zanthoxylum bungeanum, Eugenia caryophyllata, Foeniculum vulgare, was added to Naengmyeon broth with the concentration of 0.1% and 0.3% each, its effect during the preservation time of broth was investigated. pH of the extract-added broth was lower than control at the initial, but higher after 72 hours of preservation, which showed that when it added 0.1% and 0.3% of extract to the broth, pH of Kaempferia galanga L. was 4.92 and 5.08 respectively, whereas control was 4.60. Titratable acidity was lowered after 48 hours and also Kaempferia galanga L. showed the lowest acidity with 0.66 for adding 0.1% of its extract and 0.55 for 0.3% of adding, but control was 0.89 at the time of 90 hours of preservation, and then it showed to be lowered in the order of Zanthoxylum bungeanum, Eugenia caryophyllata and Foeniculum vuigare. Turbidity of each broth added the extracts of four of the medicinal plants was 7.5∼7.9 and 7.9∼8.2, respectively for 0.1% and 0.3% of concentration at the initial, but it began to lower and 90 hours later it was 8.8∼9.5 and 8.7∼9.0 respectively, whereas control was 10.8. Total viable cells(TVC) and coliform bacteria(CB) were increased with great at the 72 hours of preservation time, and Kaempferia galanga L. was the most effective, which when control was 4.8${\times}$10 CFU/ml at 72 hours, TVC was 1.7${\times}$10 CFU/ml for the addition of 0.1% of extract and 0.9${\times}$10 CFU/ml for 0.3%. CB was 3.2${\times}$10 CFU/ml for 0.1% and 1.7${\times}$10 CFU/ml for 0.3% respectively and 6.0 ${\times}$ 10 CFU/ml for control at the time of 72 hours, and it was lowered in the order of Zanthoxylum bungeanum, Eugenia caryophyllata and Foeniculum vulgare. Volatile basic nitrogen content detected that control was 2.67mg% at first, and then increased to 3.96mg% at 90 hours of preservation, but the broth added with the extract of Kaempferia galanga L. was 2.58mg% for 0.1% and 2.47mg% for 0.3% at the initial, and at 90 hours it was 3.64mg% and 3.33mg% respectively. The results of adding the extracts of four medicinal plants for the improvement of the preservation time of Naengmyeon broth, were that the most effective medicinal plant was Kaempferia galanga L. and the antimicrobial activity of the medicinal plant extracts for Naengmyeon broth was highly effective after 3 days of preservation time.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.411-418
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• 2013

The black pine bast scale, Matsucoccus thunbergianae is one of the most serious insect pest in Japanese black pine, Pinus thunbergii forest in Korea. Insecticidal activity of 10 folds hot water extracts from 7 herbal plants (Atractylodes lancea, Eugenia caryophyllata, Lonicera japonica, Melia azedarach, Quisqualis indica, Sophora flavescens and Taraxacum mongolicum) were tested against different stage of M. thunbergianae using spray method both in laboratory and field. Efficacies of herbal extracts were different depending on stage of M. thunbergianae. Q. indica sprayed with hand sprayer produced the highest corrected mortality of 95.7% on intermidiated nymph stage, however, A. lancea produced the highest corrected mortality (51.3%) on pupae of M. thunbergianae in laboratory. Q. indica and A. lancea were highly effective against female M. thunbergianae in laboratory, however, corrected mortality was lower than other stage (40.0%). Efficacy of Q. indica was similar to effective insecticide, fenitrothion 50% EC against M. thunbergianae in field trials. These results indicated that Q. indica could be an environmental friendly control agent of M. thunbergianae.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.30-34
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• 2016

To evaluate the efficacy of natural nematicides for the control of root-knot nematode in strawberry greenhouses, commercial essential oils were examined by 24-well culture plate bioassay for their nematicidal activities against second-stage juveniles and eggs of Meloidogyne hapla. Based on the mortality of M. hapla juveniles at a concentration of $125{\mu}g/mL$, the most active essential oil was Alpinia galanga (100%), followed by Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%), and Foeniculum vulgare (2.1%). Moreover, A. galanga significantly reduced hatching at 7, 14, and 21 days after treatment. The volatile constituents identified in the A. galangal oil were methyl cinnamate (87.4%), 1,8-cineole (4.4%), ${\beta}$-pinene (2.5%), ${\alpha}$-pinene (2.2%), and p-cymene (1.1%), as major constituents. Results of this study show that A. galangal essential oil and its major constituents may serve as an environmental friendly agent of a promising natural nematicide to control Meloidogyne spp.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.4
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• pp.712-718
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• 2000

The alkali extraction of anticoagulant from a clove revealed 3-to 6-fold more effective than hot-water extraction. The highest anticoagulant activity was found with 0.1 N NaOH at 7$0^{\circ}C$. The anticoagulant fractions from a clove, EC-2B and EC-2C were separated by alkali extraction, ethanol precipitation, cetavlon treatment, and ultrafiltration. The anticoagulant activities of these two fractions were, respectively, 6.57 and 8.63 times higher than those extracted with hot-water. As of the sensory evaluation, boiled pork added with EC-2B fraction revealed similar sensory acceptability to raw clove material, while EC-2C fraction had low sensory acceptability due to a mild chemical odor. Antibacterial characteristics against pathogenic microorganism of both fractions were confirmed in the control strain. The inhibitory effect of growth by EC-2B was noticed above 0.016% in S. aureus. Also, EC-2C showed the inhibitory effect at 0.004% in both E. cole and S. aureus control strains.

• Microbiology and Biotechnology Letters
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• v.38 no.3
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• pp.315-321
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• 2010

Malassezia furfur is an important causal factor for seborrheic dermatitis. Nowadays, the drugs available to treat this fungal infection are few. Several studies have documented the biological activity of essential oils. However, its antifungal properties are not completely understood, especially its anti-Malassezia activity. The aim of this study were to evaluate the effect of the plant essential oils on the growth of M. furfur using disk diffusion method and analyze by Gas chromatography-mass spectrometry (GC-MS) most active essential oils. In first screening, the 17 plant essential oils have possesses inhibitory activity against M. furfur at 2 mg/mL. Among the plant essential oils, oil of Citrus auranifoli was most active against M. furfur and its activity showed dose dependency. This anti-malassezial activity was high than that of itraconazole at 2 mg/mL. Oil of Citrus auranifolia also was phytochemically examined by GC-MS analysis, its main constituents were identified as limonene, ${\gamma}$-terpinene and terpinolene. It can be concluded that essential oils of Citrus auranifolia may have interesting applications to control fungal-derived diseases.