Abstract
When the extract of the medicinal plants, Kaempferia galanga L., Zanthoxylum bungeanum, Eugenia caryophyllata, Foeniculum vulgare, was added to Naengmyeon broth with the concentration of 0.1% and 0.3% each, its effect during the preservation time of broth was investigated. pH of the extract-added broth was lower than control at the initial, but higher after 72 hours of preservation, which showed that when it added 0.1% and 0.3% of extract to the broth, pH of Kaempferia galanga L. was 4.92 and 5.08 respectively, whereas control was 4.60. Titratable acidity was lowered after 48 hours and also Kaempferia galanga L. showed the lowest acidity with 0.66 for adding 0.1% of its extract and 0.55 for 0.3% of adding, but control was 0.89 at the time of 90 hours of preservation, and then it showed to be lowered in the order of Zanthoxylum bungeanum, Eugenia caryophyllata and Foeniculum vuigare. Turbidity of each broth added the extracts of four of the medicinal plants was 7.5∼7.9 and 7.9∼8.2, respectively for 0.1% and 0.3% of concentration at the initial, but it began to lower and 90 hours later it was 8.8∼9.5 and 8.7∼9.0 respectively, whereas control was 10.8. Total viable cells(TVC) and coliform bacteria(CB) were increased with great at the 72 hours of preservation time, and Kaempferia galanga L. was the most effective, which when control was 4.8${\times}$10 CFU/ml at 72 hours, TVC was 1.7${\times}$10 CFU/ml for the addition of 0.1% of extract and 0.9${\times}$10 CFU/ml for 0.3%. CB was 3.2${\times}$10 CFU/ml for 0.1% and 1.7${\times}$10 CFU/ml for 0.3% respectively and 6.0 ${\times}$ 10 CFU/ml for control at the time of 72 hours, and it was lowered in the order of Zanthoxylum bungeanum, Eugenia caryophyllata and Foeniculum vulgare. Volatile basic nitrogen content detected that control was 2.67mg% at first, and then increased to 3.96mg% at 90 hours of preservation, but the broth added with the extract of Kaempferia galanga L. was 2.58mg% for 0.1% and 2.47mg% for 0.3% at the initial, and at 90 hours it was 3.64mg% and 3.33mg% respectively. The results of adding the extracts of four medicinal plants for the improvement of the preservation time of Naengmyeon broth, were that the most effective medicinal plant was Kaempferia galanga L. and the antimicrobial activity of the medicinal plant extracts for Naengmyeon broth was highly effective after 3 days of preservation time.
산내, 화초, 정향, 소회향의 약용식물을 추출하여 냉면육수에 0.1%와 0.3%의 농도로 첨가했을 때 저장기간 동안 냉면육수에 미치는 영향을 살펴본 결과 육수의 pH는 이들 약용식물 처리군 중 산내 첨가군이 가장 효과적으로 무첨가군이 pH 4.60일 때, 0.1%와 0.3% 첨가시 각각 pH 4.92, pH 5.08이었다. 적정산도는 저장기간 48시간 이후부터는 산도가 현저히 낮아짐을 볼 수 있었는데, 산내의 측정치가 저장기간 90시간에 무첨가군이 0.89%일 때 0.1%첨가와 0.3% 첨가시 각각 산도 0.66과 0.55로 가장 낮았고, 다음으로 화초, 정향, 소회향 순으로 낮은 값을 보였다. 탁도는 처음에는 약용식물 첨가군이 0.1% 첨가시 7.5∼7.9, 0.3% 첨가시 7.9∼8.2였고, 무첨가군이 7.1이었다. 90시간 경과 후에는 무첨가군인 10.8, 약용식물 첨가군은 0.1%와 0.3% 첨가군이 각각 8.8∼9.5, 8.7∼9.0으로 더 낮은 값을 나타내었다. 총균수 및 대장균수는 저장기간 72시간째에 무첨가군에서 급격히 균이 증가하였고, 산내가 가장 효과적인 억제를 보였는데, 즉 총균수는 72시간째에 무첨가군이 4.8${\times}$10 CFU/ml 일 때, 0.1% 첨가시 1.7${\times}$10 CFU/ml, 0.3% 첨가시 0.9${\times}$10 CFU/ml 이었다. 대장균수는 72시간째에서는 무첨가군이 6.0 ${\times}$ 10 CFU/ml 일때, 0.1% 첨가시 3.2${\times}$10 CFU/ml, 0.3% 첨가시 1.7${\times}$10CFU/ml 이었고, 저장기간 90시간째에는 무첨가군이 6.0 ${\times}$ 10 CFU/ml 일 때 0.1% 첨가시 3.1${\times}$10 CFU/ml, 0.3% 첨가시 1.7${\times}$10 CFU/ml 로 측정되었고, 산내에 이어 화초, 정향, 소회향 순으로 효과를 나타내었다. 휘발성 염기질소량은 가장 낮은 측정치를 보인 산내의 경우 처음에 무첨가군이 2.67mg%일 때, 0.1%와 0.3% 첨가시 각각 2.58mg%, 2.47mg%이었고, 90시간 후에는 무첨가군이 3.96mg% 일 때, 0.1%와 0.3% 첨가시 각각 3.64mg%, 3.33mg%를 나타내었다. 이상의 냉면육수의 저장성 향상을 위한 산내, 화초, 정향, 소회향의 4가지 약용식물을 첨가하여 저장기간 중의 변화를 살펴본 결과 산내추출물을 첨가했을 때 가장 효과적이었으며, 저장기간 3일 이후에 항균효과가 뚜렷이 나타남을 확인하였다.