• Journal of Applied Biological Chemistry
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• 제49권3호
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• pp.82-85
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• 2006

Eucommia ulmoides bark extracts by cold water, boiling water, 100% EtOH, 70% EtOH, 100% MeOH, 70% MeOH and $CHCl_3$ were assayed for their medicinal effects. The antioxidant activity of the extracts ranged from $IC_{50}$ 125.2 to $IC_{50}\;872.7{\mu}g/ml$ in the 1,1-diphenyl-2-picrylhydrazyl (DDPH) free radical-scavenging assay, and cold water extracts had the highest antioxidant activity. $CHCl_3$ extracts had the highest inhibitory effect on angiotensin I-converting enzyme (ACE) giving inhibition of up to 56.4% at a concentration of 1 mg/ml. Extracts in 100% EtOH had the greatest inhibitory effect on $\acute{a}-amylase$ activity ($IC_{50}=174.6{\mu}g/ml$), and 70% MeOH extracts had the greatest inhibitory effect on ${\alpha}-glucosidase$ activity ($IC_{50}=14.0{\mu}g/ml$). Taken together, these results provided the in vitro evidence on the ACE, amylase and glucosidase inhibitory actions of E. ulmoides bark that form the pharmacological basis for its antihypertensive and antidiabetic action.

• Nutritional Sciences
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• 제9권4호
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• pp.330-334
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• 2006

Chemical composition of the Eucommia ulmoides bark, including extractives, proximate, mineral, fatty acid and monosaccharide compositions, was studied The most abundant mineral was calcium (533.17 mg/l00 g). $\alpha-linolenic$ acid (24.7%) and linoleic acid (24.3%), showed higher contents among the fatty acids. Major monosaccharides of E. ulmoides balk were arabinose (13.94 mg/g), xylose (18.91 mg/g) and glucose (119.7 mg/g). From the bark of E ulmoides, four compounds were isolated and their structures were elucidated as caffeic acid (I), kaempferol (II), quercetin (III) and isoquercitrin (IV) by spectroscopic analysis such as NMR and MS, including cellulose TLC and other chemical evidence such as hydrolyzation and acetylation. The antioxidant activities of four isolated compounds were evaluated by DPPH free radical scavenging, hydroxyl scavenging and reducing power assays. The results indicated that all the isolated compounds showed higher DPPH radical scavenging activity than $\alpha-tocopherol$ and BHT that were used as positive controls and these four compounds exhibited considerable reducing power and hydroxyl radical (OH) scavenging activity. Considering from the results above, it suggests that the E. ulmoides bark is a potential natural source of antioxidant material.

• 한국식품영양과학회지
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• 제27권5호
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• pp.914-919
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• 1998

This work was designed to determine the optimum extraction conditions for imporving the quality of Eucommia ulmoidesl leaf-tea. Soluble solid content was 27.7% in the tea extracted at 99.3$^{\circ}C$(extraction temperature) and 67.8 min(extraction time) which were maximum points by the ridge analysis. The extraction conditions for the maximum organoleptic scores were 72.9$^{\circ}C$ and 59.6 min in color, 80.$0^{\circ}C$ and 90.0 min in aroma, 77.8$^{\circ}C$ and 55.5 min in aftertaste, and 77.9$^{\circ}C$ and 53.1 min in overall palatability. The extraction conditions for the minimum organoleptic scores were 77.8$^{\circ}C$ and 52.7 min in astringent taste, and 75.1$^{\circ}C$ and 49.4 min in Chinese medicine taste. The optimum ranges of the conditions based on soluble solid content and overall palatability of the tea wre 75~83$^{\circ}C$ and 55~65 min. The soluble solid content and overall palatability predicted at ooptimum condition(78$^{\circ}C$ and 60 min) werw similar to experimental values.

• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.259-265
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• 2020

두충나무(Du-zhong, Eucommia ulmoides Oliver)의 잎과 껍질로 부터 얻어진 50% 에탄올 추출물의 항산화 활성을 확인하였다. 두충나무 잎과 껍질 추출물의 수율은 각각 8.1±0.31과 17.4±0.89%, 총 폴리페놀 함량은 각각 64.1±3.35와 42.4±2.38 ㎍ GAE/mg, 총 플라보노이드 함량은 각각 24.0±3.15와 36.7±3.18 ㎍ QE/mg으로 나타났다. 두충나무 잎과 껍질 추출물은 농도에 비례하여 양이온라디칼, 유리라디칼과 아질산염을 소거하였고, 환원력을 증가시켰으며 지질과산화를 억제하였다. 두충나무 잎과 껍질 추출물의 ABTS 양이온라디칼 소거에 대한 EC₅₀값은 각각 560.6±17.65와 1,357.4±8.45 ㎍/mL이었고, DPPH 유리라디칼 소거에 대한 EC₅₀값은 각각 574.2±14.70과 2,103.1±108.59 ㎍/mL로 나타났다. 또한 두충나무 잎과 껍질 추출물의 환원력에 대한 EC₅₀값은 각각 319.9±13.42와 705.9±26.08 ㎍/mL였고, 아질산염 소거에 대한 EC₅₀값은 각각 2,329.2±35.11과 5,467.6±243.92㎍/mL로 나타났다. 두충나무 잎 추출물은 74.8 ㎍/mL의 농도에서, 껍질 추출물은 177.2 ㎍/mL의 농도에서 linoleic acid의 과산화를 각각 70.0 및 79.1% 억제하였다. 두충나무 잎 추출물은 껍질 추출물에 비해 높은 항산화 활성을 보유하고 있었다.

• 식품산업과 영양
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• 제5권2호
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• pp.27-28
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• 2000

• Food Science and Biotechnology
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• 제18권2호
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• pp.475-480
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• 2009

Ultraviolet B (UVB) radiation provokes the generation of reactive oxygen species (ROS) in the cells and skin, which induce oxidative stress in the exposed cells, leading to photoaging and cancer. Using the human keratinocytes HaCaT cell line, we investigated the photoprotective effects of aucubin isolated from Eucommia ulmoides. Pretreatment with aucubin markedly suppressed UVB-induced oxidative stress, which manifests as a decrease in intracellular lipid peroxidation, elevation of catalase activity, and reduced glutathione content. In addition, aucubin significantly reduced expression of matrix metalloproteinase-1 (MMP-1) protein (54%) and mRNA. Taken together, these results suggest that aucubin may offer protection against UVB-induced oxidative stress and may be used as a potential agent in prevention of UVB-induced photoaging.

• Journal of Plant Biology
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• 제36권3호
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• pp.275-279
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• 1993

The early ontogeny of the vascular cambium in the hypocotyl of Eucommia ulmoides seedling was studied anatomically. In transverse view, the procambial cells repeated periclinal divisions and then gradually differentiated into vascular cambium; however, in the transectional plane, it was difficult to reveal the origin of cambial initials. In tangential view, the early procambial cells repeated periclinal divisions and then the procambium consisted of homogeneous short cells. Some cells in late procambium did not show transverse divisions, but elongated continuously. Thus, the procambium showed a heterogeneous structure: The long cells became fusiform initials with tapering end walls and the short cells differentiated into ray initials. Conclusively, the two types of cambial initials were gradually originated from the homogeneous cells at the early procambial stage, and they consisted vascular cambium.

• 한국식품과학회지
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• 제8권2호
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• pp.70-73
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• 1976

한국 고유차류(固有茶類)의 개발을 목적으로 두중(杜仲)나무(Eucommia ulmoides)잎의 화학성분, 침출(浸出)조건 및 가공(加工)방법을 실험한 결과 두중차(杜仲茶)는 차류(茶類)로서의 기호적(嗜好的) 특성을 충분히 구비하고 있음을 확인하였다.

• 생약학회지
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• 제6권1호
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• pp.29-34
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• 1975

Morphological characteristics of the Eucommia barks produced in Korea and China and of the barks of Euonymus japonicus $T_{HUMBERGI}$ in Korea and Euonymus pellucidifolia $H_{AGATA}$ in Taiwan were compared. The similarity in morphological features between Eucommiae Cortex produced in Korea and that of China suggests that both plants are of same botanical origin. However, the size of tissues and cells indicates that there is only one difference due to warm climatic environment. The similarity and difference of the barks of Euonymus japonicus produced in Korea and Euonymus pellucidifolia produced in Taiwan comfirmed that the two plants belong to the same genus and that they are different species.

• Natural Product Sciences
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• 제25권2호
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• pp.143-149
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• 2019

Eucommiae Cortex (EC), bark of Eucommia ulmoides, has been known as a traditional medicine to regulate hypertension and immune system. Because silk of gum in the EC blocks the release of active ingredients, EC generally has been utilized after processing with carbonization or salt-water to breakdown it. This study aimed to investigate the differences of non-processed EC and processed EC on antioxidant and anticancer properties. Antioxidant capacity was assessed by measuring the content of total polyphenols, reducing power, and ABTS radical scavenging effect. And anticancer effects were examined by evaluating the viability of pancreatic cancer cells and wound healing ability. The results demonstrated that processed EC contained more content of polyphenols and exhibited more potent reducing power and radical scavenging effect than non-processed EC. In addition, processed EC more efficiently inhibited proliferation and migration of pancreatic cancer cells. These results suggest that processing of medicinal plants can improve the biological properties such as antioxidant or anticancer activity, which may lead to the development of herbal medicine treatment technology.