Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Effects of Aucubin Isolated from Eucommia ulmoides on UVB-induced Oxidative Stress in Human Keratinocytes HaCaT

  • Ho, Jin-Nyoung (Department of Food and Biotechnology, Han-Seo University) ;
  • Cho, Hong-Yon (Department of Food and Biotechnology, Korea University) ;
  • Lim, Eun-Jeong (Department of Food and Nutrition, Hanyang Women's College) ;
  • Kim, Hye-Kyung (Department of Food and Biotechnology, Han-Seo University)
  • Published : 2009.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Ultraviolet B (UVB) radiation provokes the generation of reactive oxygen species (ROS) in the cells and skin, which induce oxidative stress in the exposed cells, leading to photoaging and cancer. Using the human keratinocytes HaCaT cell line, we investigated the photoprotective effects of aucubin isolated from Eucommia ulmoides. Pretreatment with aucubin markedly suppressed UVB-induced oxidative stress, which manifests as a decrease in intracellular lipid peroxidation, elevation of catalase activity, and reduced glutathione content. In addition, aucubin significantly reduced expression of matrix metalloproteinase-1 (MMP-1) protein (54%) and mRNA. Taken together, these results suggest that aucubin may offer protection against UVB-induced oxidative stress and may be used as a potential agent in prevention of UVB-induced photoaging.

Keywords

References

  1. Psotova J, Svobodova A, Kolarova H, Walterova D. Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes. J. Photoch. Photobio. B 84: 167-174 (2006) https://doi.org/10.1016/j.jphotobiol.2006.02.012
  2. Na MK, Min BS, An RB, Song KS, Seong YH, Bae K. Effect of Astilbe koreana on ultraviolet B (UVB)-induced inflammatory response in human keratinocytes. Biol. Pharm. Bull. 27: 1301-1304 (2004) https://doi.org/10.1248/bpb.27.1301
  3. Cimino F, Ambra R, Canali R, Saija A, Virgili F. Effect of cyaniding-3-O-glucoside on UVB-induced response in human keratinocytes. J. Agr. Food Chem. 54: 4041-4047 (2006) https://doi.org/10.1021/jf060253x
  4. Nishi J, Ogura R, Sugiyama M, Hidaka T, Kohno M. Involvement of active oxygen in lipid peroxide radical reaction of epidermal homogenate following ultraviolet light exposure. J. Invest. Dermatol. 97: 115-119 (1991) https://doi.org/10.1111/1523-1747.ep12478534
  5. Erden IM, Kahramant A, Kokent T. Beneficial effects of quercetin on oxidative stress induced by ultraviolet A. Clin. Exp. Dermatol. 26: 536-539 (2001) https://doi.org/10.1046/j.1365-2230.2001.00884.x
  6. Oh JH, Chung AS, Steinberenner H, Sies H, Brenneisen P. Thioredoxin secreted upon ultraviolet A irradiation modulates activities of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human dermal fibroblasts. Arch. Biochem. Biophys. 423: 218-226 (2004) https://doi.org/10.1016/j.abb.2003.12.026
  7. Fisher GJ, Talwar HS, Lin J, Lin P, McPillips F, Wang Z, Li X, Wan Y, Kang S, Voorhees JJ. Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo. J. Clin. Invest. 101: 1432-1440 (1998) https://doi.org/10.1172/JCI2153
  8. Sudel KM, Venzke K, Knussmann-Hartig E, Moll I, Stab F, Wenck H, Wittern K, Gercken G, Gallinat S. Tight control of matrix metalloproteinase-1 activity in human skin. Photochem. Photobiol. 78: 355-360 (2003) https://doi.org/10.1562/0031-8655(2003)078<0355:TCOMMA>2.0.CO;2
  9. Sternlicht MD, Werb Z. How matrix metalloproteinases regulate cell behavior. Annu. Rev. Cell Dev. Bi. 17: 463-516 (2001) https://doi.org/10.1146/annurev.cellbio.17.1.463
  10. Steinbrenner H, Ramos MC, Stuhlmann D, Sies H, Brenneisen P. UVA-mediated downregulation of MMP-2 and MMP-9 in human epidermal keratinocytes. Biochem. Bioph. Res. Co. 308: 486-491 (2003) https://doi.org/10.1016/S0006-291X(03)01430-X
  11. Katiyar SK, Ahmad N, Mukhtar H. Green tea and skin. Arch. Dermatol. 136: 989-994 (2000) https://doi.org/10.1001/archderm.136.8.989
  12. Rodriguez J, Yanez J, Vicente V, Alcaraz M, Benavente-Garcia O, Castillo J, Lorente J, Lozano JA. Effects of several flavonoids on the growth of B16F10 and SK-MEL-1 melanoma cell lines:Relationship between structure and activity. Melanoma Res. 12: 99-107 (2002) https://doi.org/10.1097/00008390-200204000-00001
  13. Yen GC, Hsieh CL. Antioxidant activity of extracts from Du-Zhong (Eucommia ulmoides Oliv.) toward various lipid peroxidation models in vitro. J. Agr. Food Chem. 46: 3952-3957 (1998) https://doi.org/10.1021/jf9800458
  14. Yen GC, Hsieh CL. Reactive oxygen species scavenging activity of Du-Zhong (Eucommia ulmoides Oliv.) and its active compounds. J. Agr. Food Chem. 48: 3431-3436 (2000) https://doi.org/10.1021/jf000150t
  15. Ho JN, Lee YH, Lee YD, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY. Inhibitory effects of aucubin isolated from Eucommia ulmoides against UVB-induced matrix metalloproteinase-1 production in human skin fibroblasts. Biosci. Biotech. Bioch. 69: 2227-2231 (2005) https://doi.org/10.1271/bbb.69.2227
  16. Jeong HJ, Koo HN, Na HJ, Kim MS, Hong SH, Eom JW, Kim KS, Shin TY, Kim HM. Inhibition of TNF-$\alpha$ and IL-6 production by aucubin through blockade of NF-$\kappa$B activation in RBL-2H3 mast cells. Cytokine 18: 252-259 (2002) https://doi.org/10.1006/cyto.2002.0894
  17. Moon HI, Lee J, Zee OP, Chung JH. Flavonoid compounds from Viola hondoensis and their effect on matrix metalloproteinase-1 in ultraviolet irradiation of cultured human skin fibroblasts. Food Sci. Biotechnol. 14: 143-146 (2005)
  18. Jones SA, McArdle F, Jack CI, Jackson MJ. Effect of antioxidant supplementation on the adaptive response of human skin fibroblasts to UV-induced oxidative stress. Redox. Rep. 4: 291-299 (1999) https://doi.org/10.1179/135100099101535133
  19. Chan WH, Wu CC, Yu JS. Curcumin inhibits UV irradiationinduced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells. J. Cell Biochem. 90: 327-338 (2003) https://doi.org/10.1002/jcb.10638
  20. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase, and glutathione S-transferase activities in rat lung and liver. Biochim. Biophys. Acta 582: 67-78 (1979) https://doi.org/10.1016/0304-4165(79)90289-7
  21. Ryoo YW, Suh SI, Mun KC, Kim BC, Lee KS. The effects of the melatonin on ultraviolet-B irradiated cultured dermal fibroblasts. J. Dermatol. Sci. 27: 162-169 (2001) https://doi.org/10.1016/S0923-1811(01)00133-5
  22. Aebi H. Catalase. Method Enzymol. 105: 125-126 (1985)
  23. McCord J, Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (heterocuprein). J. Biol. Chem. 244: 6049-6055 (1969)
  24. Philips N, Smith J, Keller T, Gonzalez S. Predominant effects of Polypodium leucotomos on membrane integrity, lipid peroxidation, and expression of elastin and matrixmetalloproteinase-1 in ultraviolet radiation exposed fibroblasts, and keratinocytes. J. Dermatol. Sci. 32: 1-9 (2003) https://doi.org/10.1016/S0923-1811(03)00042-2
  25. Nakamura T, Ishikawa T, Nanashima N, Miura T, Nozaka H, Nakaoka R, Sato T. 4-Methylumbelliferone induces the expression of membrane type 1-matrix metalloproteinase in cultured human skin fibroblasts. Biochem. Bioph. Res. Co. 298: 646-650 (2002) https://doi.org/10.1016/S0006-291X(02)02516-0
  26. Fusening NE, Boukamp P. Multiple stages and genetic alterations in immortalization, malignant transformation, and tumor progression of human skin keratinocytes. Mol. Carcinogen. 23: 144-158 (1998) https://doi.org/10.1002/(SICI)1098-2744(199811)23:3<144::AID-MC3>3.0.CO;2-U
  27. Catani MV, Rossi A, Costanzo A, Sabatini S, Levrero M, Melino G, Avigliano L. Induction of gene expression via activator protein-1 in the ascorbate protection against UV-induced damage. Bichem. J. 356: 77-85 (2001) https://doi.org/10.1042/0264-6021:3560077
  28. Fischer TW, Zbytek B, Sayre RM, Apostolov EO, Basnakian AG, Sweatman TW, Wortsman J, Elsner P, Slominski A. Melatonin increases survival of HaCaT keratinocytes by suppressing UVinduced apoptosis. J. Pineal Res. 40: 18-26 (2006) https://doi.org/10.1111/j.1600-079X.2005.00273.x
  29. Afaq F, Mukhtar H. Botanical antioxidants in the prevention of photocarcinogenesis and photoaging. Exp. Dermatol. 15: 678-684 (2006) https://doi.org/10.1111/j.1600-0625.2006.00466.x
  30. F'guyer S, Afaq F, Mukhtar H. Photochemoprevention of skin cancer by botanical agents. Photodermatol. Photo. 19: 56-72 (2003) https://doi.org/10.1034/j.1600-0781.2003.00019.x
  31. Afaq F, Adhami VM, Mukhtar H. Photochemoprevention of ultraviolet B signaling and photocarcinogenesis. Mutat. Res. 571:153-173 (2005) https://doi.org/10.1016/j.mrfmmm.2004.07.019
  32. Rabe JH, Mamelak AJ, McElgunn PJS, Morison WL, Sauder DN. Photoaging: Mechanisms and repair. J. Am. Acad. Dermatol. 55: 1-19 (2006) https://doi.org/10.1016/j.jaad.2005.05.010
  33. Tarozzi A, Marchesi A, Hrelia S, Angeloni C, Andrisano V, Fiori J, Cantelli-Forti G, Hrelia P. Protective effects of Cyanidin-3-O-$\beta$-glucopyranoside against UVA-induced oxidative stress in human keratinocytes. Photochem. Photobiol. 81: 623-629 (2005) https://doi.org/10.1562/2004-06-14-RA-200.1
  34. Weisiger RA, Fridovich I. Superoxide dismutase: Organelle specificity. J. Biol. Chem. 248: 3582-3592 (1973)
  35. Dissemond J, Schneider LA, Brenneisen P, Briviba K, Wenk J, Wlaschek M, Scharffetter-Kochanek K. Protective and determining factors for the overall lipid peroxidation in ultraviolet A1-irradiated fibroblasts: in vitro and in vivo investigations. Brit. J. Dermatol. 149:341-349 (2003) https://doi.org/10.1046/j.1365-2133.2003.05457.x
  36. Jagetia GC, Reddy TK, Venkatesha VA, Kedlaya R. Influence of naringin on ferric iron induced oxidative damage in vitro. Clin. Chim. Acta 347: 189-197 (2004) https://doi.org/10.1016/j.cccn.2004.04.022
  37. Wang H, Kochevar IE. Involvement of UVB-induced reactive oxygen species in TGF-$\beta$ biosynthesis and activation in keratinocytes. Free Radical Bio. Med. 38: 890-897 (2005) https://doi.org/10.1016/j.freeradbiomed.2004.12.005
  38. Fischer TW, Scholz G, Knoll B, Hilper U-C, Elsner P. Melatonin reduces UV-induced reactive oxygen species in dose-dependent manner in IL-3-stimulated leukocytes. J. Pineal Res. 31: 39-45 (2001) https://doi.org/10.1034/j.1600-079X.2001.310106.x
  39. Kim HH, Shin CM, Park CH, Kim KH, Cho KH, Eun HC, Chung JH. Eicosapentanoic acid inhibits UV-induced MMP-1 expression in human dermal fibroblasts. J. Lipid Res. 46: 1712-1720 (2005) https://doi.org/10.1194/jlr.M500105-JLR200
  40. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379: 335-339 (1996) https://doi.org/10.1038/379335a0
  41. Lee YS, Jin DQ, Beak SM, Lee ES, Kim JA. Inhibition of ultraviolet-A-modulated signaling pathways by asiatic acid and ursolic acid in HaCaT human keratinocytes. Eur. J. Pharmacol. 476:173-178 (2003) https://doi.org/10.1016/S0014-2999(03)02177-0
  42. Park CH, Lee MJ, Kim JP, Yoo ID, Chung JH. Prevention of UV radiation-induced premature skin aging in hairless mice by the novel compound melanocin A. Photochem. Photobiol. 82: 574-578 (2006) https://doi.org/10.1562/2005-07-26-RA-623