• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.419-427
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• 2013

The insecticidal activity of 124 plant essential oils and control efficacy of six experimental spray formulations (SF) containing 0.25, 0.5, 1, 2.5, 5, and 10% of the selected oils was examined against both nymph and adult of the citrus flatid planthopper, Metcalfa pruinosa using direct contact applications (leaf dipping and spray). Reponses varied according to dose (1,000 and 500 mg/L). When exposed at 1,000 mg/L for 24 h using leaf dipping assay, 19 essential oils showed strong mortality (100%) among 124 essential oils screened. At 500 mg/L, 100% mortality was observed in cinnamon technical, cinnamon green leaf, cinnamon #500, cassia tree, citronella java and pennyroyal followed by origanum, thyme white, grapefruit, savory, fennel sweet, aniseed and cinnamon bark showed considerable mortality (93.3-80%) against nymphs of M. pruinosa. The moderate mortality (73.3-60%) was found in thyme red, tagetes, calamus, lemoneucalptus and geranium. Oils applied as SF-10% sprays provided 100 % mortality against adult M. pruinosa. One hundred mortalities were achieved in cinnamon technical at >SF-0.5 formulation, in cinnamon #500, cinnamon green leaf and penny royal at >SF-2.5. To reduce the level of highly toxic synthetic insecticides in the agricultural environment, the active essential oils as potential larvicides could be provided as an alternative to control M. pruinosa populations.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.11 no.3
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• pp.344-352
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• 2004

Purpose: The purpose of this study was to identify the effect of aroma therapy on exam syndromes and temporary fatigue of senior high school students who are preparing to take college entrance exams. Method: This study was a pre-experimental research using a one-group pre-post test design and was conducted between April and July 2003 with 35 high school students. A pre-post test was used to measure long-term exam syndromes and temporary fatigue during the first period of school. Paired t-test was used. Aromas were given using an aroma electrical lamp, aroma necklet with basic oils of rosemary and lemon. According to the condition of the students, one or two oils were added: peppermint, pine, eucalyptus and Clary-Sage. Results: After two months (May, June), the result of administering aroma therapy to senior high school students showed in June there was a decrease in principal exam syndromes such as eye fatigue, headache, shoulder pain, neck stiffness, back pain, and common cold, and there was an increase Un study concentration during the two months. However, in May, there were no significant effects. The reason may have been a strong extraneous factor from midterm exams that would affect their college entrance. Conclusion: According to these results, if we use aroma oil carefully, with evidence-based applications, aroma therapy can have a positive effect on senior high school students who are preparing for the college entrance exams.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.264-268
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• 2007

Three German Shepherd dogs (5 years old, 30 kg, male; 4 years old, 23 kg, female; 4 years old, 24 kg, female) with history of acute vomiting, hemorrhagic diarrhea, anorexia and dullness were admitted to veterinary clinic in the military working dogs training corp (MWDTC). All of them showed same symptoms after ingestion the $'PREVNTIC^{(R)}'$ collar to control ecto-parasites infection in field. One dog was operated by gastrotomy and the $5{\sim}6$ pieces of the $'PREVNTIC^{(R)}'$ collar were removed. The dog was completely cured by intensive care within 2 hours after $'PREVNTIC^{(R)}'$ collar ingestion. However, two dogs were operated by gastrotomy and intensive care over 24 hours after $'PREVNTIC^{(R)}'$ collar ingestion but died. In autopsy, the $5{\sim}6$ pieces of hard materials were found in the stomach, and severe thickness, congestion hemorrhage and swelling of the stomach walls and mucous membrane were observed. In these cases, it was suggested that the first aid of treatment such as gastrotomy within two hours after $'PREVNTIC^{(R)}'$ collar ingestion as soon as possible was helpful for attenuating of toxicosis.

• Parasites, Hosts and Diseases
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• v.59 no.5
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• pp.465-471
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• 2021

Culicoides biting midges (Diptera: Ceratopogonidae) are hematophagous arthropod vectors that transmit epizootic arthropod-borne viruses (arboviruses). Arboviruses are recognized as causes of pregnancy loss, encephalomyelitis, and congenital malformations in ruminants. Therefore, continuous monitoring and control of Culicoides, which causes significant damage to industrial animals are necessary. We performed attraction and repellent tests in Culicoides using various essential oils, cow dung, and carbon dioxide (CO₂). Culicoides tended to move more to cow dung (60.8%, P<0.0001) and CO₂ (63.8%, P<0.01). To the essential oils as repellents, 26.1% (P<0.0001), 18.7% (P<0.001), and 25.5% (P<0.01) of the Culicoides moved to the lavender, lemongrass, and eucalyptus chamber, respectively. The Culicoides that moved to the 3 essential oils chambers showed markedly low activity. Collectively, it was showed that Culicoides tended to be attractive to cow dung and CO₂, and repellent from the 3 essential oils.

• Journal of The Korean Society of Integrative Medicine
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• v.10 no.4
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• pp.103-111
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• 2022

Purpose : In recent years, essential oils have been produced using natural extracts for various uses. Their functionality is currently being tested not only for cosmetics and perfumes but also for other categories of products. Therefore, this study verified their antibacterial effects on S. mutans, P. gingivalis, and L. gasseri which are the representative strains that cause oral diseases. Methods : Eighteen types of natural essential oils were made at a concentration of 50 % (v/v) using Tween 20, and their antibacterial effects were verified by applying S. mutans, P. gingivalis, and L. gasseri. The antibacterial effects were measured with the disc diffusion method. All the experiments were repeated three times, and the mean value of three measurement values for each variable was used for data analysis. A one-way analysis of variance was conducted using these mean values. Results : Of the eighteen types of essential oils tested, sixteen types showed antibacterial effects on S. mutans, and sixteen and fifteen types exhibited antibacterial effects on P. gingivalis and L. gasseri respectively. The types of essential oils with high-level antibacterial activities were geranium, may chang, and bergamot for S. mutans, lemongrass, bergamot, and eucalyptus for P. gingivalis, and lemongrass, machan, and geranium for L. gasseri in order of antibacterial effect. This result was statistically significant (p<.001). In addition, in the case of mandarin oil, it was found that there was no antibacterial activity in all three strains. Conclusion : This study proved the antibacterial activities of essential oils, which are used for various purposes in daily life, against dental caries and periodontal diseases. The study results will likely be applied to different prevention programs for oral health and broadly used to develop products such as oral care items and dentifrices.

• Microbiology and Biotechnology Letters
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• v.38 no.1
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• pp.93-104
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• 2010

In this study, we investigated the effects of frankincense essential oil (BSEO) on the immune cell change in the lung, BALF and PBMC using a mouse model of asthma. BALB/c mice after intraperitoneal OVA sensitization (day 1) were challenged intratracheally with OVA on day 14. Then, the asthma was induced by repeated OVA inhalation challenged. The asthma induced mice group inhaled 0.3% BSEO for 30 minutes per trial, three times a week, for 8 weeks using the nebulizer. After 12 weeks from the experiment, the mice was killed and the lung, bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cell (PBMC) were obtained. Next, the change of immune cells inside the separated tissues was observed to identity the effects of BSEO on the allergic asthma mice. In conclusion, the hypersensitive reaction of airway to the bronchoconstrictor in the allergic asthma induced mice was effectively suppressed in Frankincense group, in Bermagot, Eucalyptus, Chamomile, Marjoram and Frankincense groups, the natural aromatic essential oil groups. Furthermore, it was also confirmed that the weight of lung, total number of alveolus cells and the number of BALF, MNL and DLN increased after inducing allergic asthma were reduced. BSEO suppressed the percentage of $CD3e^+/CD19^-$, $B220^+/CD23^+$ and $CD11b^+/Gr-1^+$ cells in the lung tissue of allergic asthma mice. Moreover, BSEO also reduced the percentage of $CD4^+/CD8^-$, $B220^+/CD23^+$ and $CD3^+/CCR3^+$ cells in BALF. In addition, the percentage of $CD3e^+/CD19^-$, $CD3^+/CD69^+$ and $B220^+/CD23^+$ cells in PBMC was reduced. The results of this study indicate that BSEO would be effective to treat allergic asthma by the immune control suppressing the activity of immune cells in each tissue.