• 산업식품공학
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• 제21권3호
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• pp.286-291
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• 2017

약용식물의 열수 추출물이 in vitro에서 alcohol dehydrogenase (ADH)와 aldehyde dehydrogenase (ALDH)의 활성에 미치는 영향을 확인하였다. 약용식물에 20배의 증류수를 넣고 $80^{\circ}C$에서 8시간 추출하여 얻어진 추출액을 시료로 사용하였다. 50종의 약용식물 중에서 마늘과 육계 추출물이 숙취해소 천연소재로서의 활용 가능성이 가장 높게 나타났다. 마늘 추출물은 ADH에 비해 ALDH의 활성을 2배 이상 촉진시킴으로써 acetaldehyde의 분해가 잘 되게 하였다. 육계 추출물은 ALDH의 활성에 비해 ADH의 활성을 획기적으로 저해함으로써 acetaldehyde의 생성을 크게 억제하였다. 육계 추출물은 농도에 비례하여 ADH와 ALDH의 활성을 저해하였으며, $45.33{\mu}g/mL$의 농도에서 ADH의 활성을 52.8% 저해하였고 ALDH의 활성을 11.0% 저해하였다.

• Food Science and Biotechnology
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• 제18권2호
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• pp.419-424
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• 2009

Water and 70% ethanol extracts obtained from a sea cucumber (Stichopus japonicus) body wall by heat reflux or pressurized solvent extraction showed 2,2-diphenyl-1-picrylhydrazyl ($DPPH^{\cdot}$) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation ($ABTS^{{\cdot}+}$) scavenging activities comparable to those of fruits and vegetables. The highest activities were observed for the water extract from heat reflux extraction. Crude saponins exhibited higher radical scavenging activities than the soluble matters in the extracts. However, they were responsible for only about 3 to 15% of the scavenging activities of the extracts. Total phenolic contents showed a significant correlation with $DPPH^{\cdot}$ scavenging activities, suggesting a significant contribution of phenolic constituents to the antioxidant properties of the extracts. However, total flavonoid contents showed little correlation with the radical scavenging activities. The results suggest that the water or 70% ethanol extract obtained from sea cucumber body wall by simple heat reflux extraction could provide considerable antioxidant benefits.

• Advances in Traditional Medicine
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• 제1권1호
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• pp.89-96
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• 2000

A human hepatoma cell line, Hep G2 cells are a reliable for the study of alcohol-induced hepatotoxicity. In this study, the author investigated the effect of an aqueous extract of Asparagus $cochinchinensis_{MERRIL}$ (Liliaceae) roots (ACAE) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. ACAE dose-dependently inhibited the EtOH-induced tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ secretion. ACAE also inhibited the EtOH and $TNF-{\alpha}-induced$ cytotoxicity. Furthermore, the author found that ACAE inhibited the $TNF-{\alpha}-induced$ apoptosis of Hep G2 cells. These results suggest that ACAE may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.

• 한국약용작물학회지
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• 제12권4호
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• pp.309-314
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• 2004

Echinacea의 추출물 및 분획물들의 정상세포에 대한 세포독성을 측정한 결과, 0.5 mg/ml이상의 농도에서 정상세포의 생육이 20% 가까이 저해되었으며, 비교적 높은 농도인 1.0 mg/ml의 농도에서도 70%이상의 세포 생존을 나타내었다. 유방암 세포주인 MCF7에 대한 생육억제 활성은 0.5 mg/ml의 농도에서 물 추출물의 butanol 획분과 ethanol 추출물의 ethyl acetate 획분에서 각각 87%와 81%로 가장 높은 억제활성을 나타내었으며, 인간 간암세포주인 Hep3B에 대한 생육억제능은, 물과 ethanol 추출물의 butanol 획분에서 0.5 mg/ml의 농도에서 81%와 63%의 생육 억제능을 나타내었으며, 1.0 mg/ml의 농도에서는 수층획분을 제외하고는 대부분 70% 이상의 높은 생육억제능을 나타내었다. 위암세포인 AGS의 경우 물 추출물의 butanol 획분에서 0.5 mg/ml의 농도에서 80%의 높은 억제율을 나타내었으며, 1.0 mg/ml의 농도에서는 각 추출물의 수층 획분과 ethanol 추출물의 ethyl acetate 획분을 제외하고는 80% 이상의 높은 생육억제 활성을 나타내었다. 폐암세포인 A549에 대한 생육억제능은 물과 ethaonl 추출물의 butanol 획분에서 1.0 mg/ml의 농도에서 92%와 87%로 높게 나타났다. 이전까지 Echinacea에 관해서 보고된 것은 그리 많지 않으며, 본 논문에서 Echinacea의 추출물 및 분획물들에 관해 실험한 내용에 관한 것은 전무한 것으로 사료되어져, 이용에 있어서 본 논문에서 실험한 결과를 바탕으로 Echinacea의 항암활성 효과를 통하여 앞으로의 이용에 다양하게 이용될 수 있음을 알린 것이며, 아울러 Echinacea의 추출물들을 이용시 본 눈문에서 실험한 결과물인 물과 ethanol 추출물 모두 butanol 분획에서 그 효과가 가장 높았으므로 이를 사용한 다양한 암세포 사멸효과뿐만이 아니라 면역 활성 증진 및 여러 생리활성의 이용에 사용되도록 하여야 할 것이다.

• Journal of Applied Biological Chemistry
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• 제51권4호
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• pp.148-154
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• 2008

Excessive alcohol use causes oxidative stress in the liver, and antioxidant therapy has been an attractive approach for the treatment of ethanol-induced liver damage. The present study examined the hepatoprotective effect of Sasa quelpaertensis Nakai (Korean name, Jeju-Joritdae) in C57BL/6 mice intoxicated with ethanol. Mice were intraperitoneally administered with ethanol alone, or together with test materials three times at 12-h intervals. At 3 h after the last dosing, hepatotoxicity was assessed based on serum activities of aspartate aminotransferase and alanine aminotransferase, and hepatic contents of thiobarbituric acid-reactive substances and glutathione. Sasa quelpaertensis extract mitigated the acute ethanol hepatotoxicity as effectively as silymarin. Its n-butanol fraction was more active than methylene chloride or aqueous fraction. p-Coumaric acid, a major constituent of S. quelpaertensis, was found to effectively prevent the ethanol-induced hepatotoxicity. These data suggest that S. quelpaertensis and p-coumaric acid could be useful for the prevention of liver disease caused by alcohol abuse.

• Journal of Ginseng Research
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• 제44권1호
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• pp.44-49
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• 2020

Background: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOE method for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium was established in this article. Methods: The SOE system comprising ethanol and sodium carbonate was selected to extract ginsenosides from the enzymatic hydrolysates of Panax quinquefolium, and HPLC was applied to analyze the ginsenosides. Results: The optimized extraction conditions were as follows: the aqueous two-phase extraction system comprising ethanol, sodium carbonate, ethanol concentration of 41.51%, and the mass percent of sodium carbonate of 7.9% in the extraction system under the experimental condition. Extraction time had minor influence on extraction efficiency of ginsenosides. The results also showed that the extraction efficiencies of three ginsenosides were all more than 90.0% only in a single step. Conclusion: The proposed method had been successfully applied to determine ginsenosides in enzymatic hydrolysate and demonstrated as a powerful technique for separating and purifying ginsenosides in complex samples.

• 한국식품저장유통학회지
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• 제4권3호
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• pp.311-315
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• 1997

Proximate compositions of black rice and antioxidative effects of its methanol extract on refined perilla oil were investigated. Black rice seeds were composed of water 12.4%, crude protein 7.9%, crude fat 2.2%, crude fiber 1.0%, ash 1.4% and N-free extract 75.1%. The hexane and 80% methanol fractions in yields were higher than 80% ethanol and chloroform. In AOM(active oxygen method) test, antioxidative effects of black rice added to the refined perilla oil was increased with dose-dependent fashion in concentration of the black rice extracts. Among several extracts tested, 80% aqueous methanol extract showed the highest antioxidative activity, followed by hexane, chloroform, in that order. The peroxide value and TBA value for their antioxidation stability were also lower than that of control. This result was similar to that of AOM test.

• 한국약용작물학회지
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• 제10권4호
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• pp.269-272
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• 2002

Ethanol and butanol fractionation of E. senticosus showed strong anti-oxidant activity, and methanol and water extracts also had high anti-oxidant activity. The anti-oxidant activities in ethanol and butanol fractionation were higher than or similar to those of ${\alpha}-tocopherol$. The cytotoxic effect of root extract of E. senticosus was evaluated on seven different human cancer cell lines, The extracts of leaf and stem of E. senticosus also had strong antioxidant activity, but the antioxidant activity in root extract was higher than those in leaf and stem extracts. Methanol, hexane, and aqueous fraction layer had much higher inhibitory activities on lipid peroxidation in rat liver microsomes compared with ${\alpha}-tocopherol$. The effect of root extract of E. senticosus was evaluated on six human cancer cell lines. The values of 50% growth inhibition $(GI_{50})$ for the extracts were mostly below $30{\mu}g/ml$, and the extracts are considered as active inhibitory compounds on cancer cells.

• 한국약용작물학회지
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• 제18권5호
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• pp.315-322
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• 2010

This study was carried out to investigate the antimicrobial activities of organic extracts obtained from the fruit of Thuja orientalis L. The native fruits in Korea were collected and extracted by 80% ethanol, and the extract was sequentially fractionated with n-hexane, chloroform, ethylacetate, and butanol. The fraction yields of n-hexane, chloroform, ethylacetate, butanol and water of ethanol extract were 10.15%, 10.05%, 1.45%, 45.35% and 27.55%, respectively. n-Hexane-soluble fraction showed the highest antibacterial activity against gram positive bacteria, while the chloroform, ethylacetate, butanol and aqueous fractions did not show any antibacterial activity. Minimum inhibitory concentration (MICs) on Staphyloycoccus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction were $100\;{\mu}g$, $500\;{\mu}g$ and $50\;{\mu}g$/disc, respectively. The antibacterial activity was not destroyed by heating at 80, 100, $120^{\circ}C$ for 30 min and was not affected by pH. In the inhibitory test against the Staphylococcus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction showed potent growth inhibition at the concentration of 0.1 and $0.5\;{\mu}g/mL$ for 12~24 hours and n-hexane-soluble fraction did not show any mutagenic activity.

• 한국식품영양과학회지
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• 제33권4호
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• pp.711-715
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• 2004

홍국균이 생산하는 색소를 상업적으로 이용하기 위한 기초연구로서 Monascus purpureus CBS 281.34 80% ethanol 가용성 색소의 열 pH, 유기산 존재 시의 안정성을 알아보았다. 또한 ethanol 가용성 색소 추출물에 증류수를 가해 수분 함량이 0∼80%가 되도록 하고 수용성 색소와 함께 저장하면서 이들의 안정성도 검토하였다. 80% ethanol 가용성 홍국색소는 1$0^{\circ}C$의 저온에서 4주간 저장 시 9.74%의 분해율을 보였으나, 60∼8$0^{\circ}C$의 고온에서는 24시간과 12시간 저장 시 각각 23.06%와 30.36%나 분해되어 색소의 안정성은 단기간 내 크게 저하하였다. pH 2와 pH 10에서의 80% ethanol 가용성 색소 분해율은 pH 4∼8보다 높았고, 0.6% citric acid, 6% acetic acid 및 1.5% lactic acid를 가해 pH 4로 조정하여 4주간 저장 시에는 대조구와 대조구와의 차이가 가장 큰 acetic acid 하에서의 분해율과의 차이도 6.08%로 거의 나타나지 않아 80% ethanol 가용성 홍국 색소는 유기산하에서 비교적 안정한 것으로 사료되었다. 또한 ethanol 가용성 색소에 증류수를 가해 저장하면 색소 안정성은 저하하였는데, 색소 분해율은 수분함량 20%에서 가장 높았고 80%에서 가장 낮았다. 한편 수중에서의 ethanol 가용성 색소는 2$0^{\circ}C$보다 1$0^{\circ}C$에서 현저하게 안정하였다.