• Natural Product Sciences
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• v.16 no.4
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• pp.259-264
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• 2010

The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural anti-inflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-$\alpha$) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).

• Health Policy and Management
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• v.4 no.2
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• pp.111-125
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• 1994

In developing total hospital information system, large amount of time and expense are to be spent while its results are likely to lead itself to end-users' dissatisfaction. Some of the main complaints on the part of end-users come from insufficient consideration of end-users environment as well as inappropriate representation of their requirement in the system alalysis and design. This papre addresses some advantages of Essential Logical Modeling Process for better analysis and design, explaining by example the developmental process of the Patent Management and Accounting System for a tertiary care hospital. In the case, the Essential Model, suggested by McMenamin and Palmer, proved to be an effective tool for clear separation of analysis and design phase and for better communication among system developers and with end-users. The modeling process itself contributed to better program modularity as well, shown in a Structured Chart. Difficulties in learning how to identify' essential activities' for the modeling practice were experienced in the beginnins stage, which were, however, overcome by elaborating some heuristic guideling and by rdferring to necessary tools including State Transition Diagram, Control Flow Diagram, and so many. While full evaluation of the Essential Model usag remains to wait till the completion of the case project, its strengt in making clear distinction between analysis and design phase was enough to be attractive to system analysts. The model concepts are open to many further application fields, particularly such areas as business re engineering, process remodeling, office automation, and organizational restructuring.

• Journal of the Korean Applied Science and Technology
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• v.24 no.2
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• pp.99-108
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• 2007

The cosmetic and toiletries are necessary health care & household for common life. However we need antiseptic which is effecting harmlessly to the human body. There are propolis, Lavender, Lemon, essential oil in the natural antiseptic materials. This work proceeded design Natural-antiseptic system with three materials as above-mentioned. Natural-antiseptic system was accomplished with propolis (2%), Lavender essential oil (0.3%), Lemon essential oil (0.3%) safety out of Polysorbate 20 (0.5%), Polysorbate 80 (0.5%), PEG (60) hydrogenated castor oil (0.45%), ethanol (5%). The antimicrobial test was experimented on E. coli and Bacillus stearothermophilus. In this antimicrobial test, we found that the effect of antisepsis against E. coli and Bacillus stearothermophilus with propolis 0.3%, Lavender essential oil 0.045% and Lemon essential oil 0.045% was improved. Therefore could expect Natural-antiseptic system product for moisturizing skin toner for face, nourishing essence and wet tissue for clean other things.

• Journal of the Korean Wood Science and Technology
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• v.22 no.3
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• pp.59-67
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• 1994

The essential oils of leaves and resins from P. densiflora and P. koraiensis were analyzed to identify their components. After each retention times of 45 known terpenoids were dertermined with a fixed analytical condition by GC the essential oil compounds of leaves and resins were identified by comparing their retention times with the retention times of known standards. To confirm these results the essential oil components of leaves from P. koraiensis were analized by 2 different GC/MS. According to these results, 36 terpenoids in essential oils of leaves from P. densiflora and P. koraiensis were identified and 15 terpenoids and 22 terpenoids were identified from P. koraiensis resin and P. densiflora resin, respectively. The major components which are more than 2% of total amaunt of volatile components were as follows: 1. The major terpenoids of leaves from red pine. ${\alpha}$-pinene, camphene, ${\beta}$-pinene, D-limonene, ${\beta}$-phellandrene, myrcene, terpinolene, ${\alpha}$-terpineol. 2. The major terpenoids of leaves from korean pine. ${\alpha}$-pinene, camphene, myrcene, D-limonene, 3-carene, terpinolene, bornyl acetate, ${\beta}$-caryophyllene, ${\alpha}$-terpineol, borneol, ${\delta}$-cardinene. 3. The major terpenoids of resin from red pine. ${\alpha}$-pinene, ${\beta}$-pinene, myrcene, ${\beta}$-phellandrene, linalool, linalyl acetate. 4. The major terpenoids of resin from korean pine. ${\alpha}$-pinene, ${\beta}$-pinene, D-limonene, ${\beta}$-caryophyllene, phytol.

• The Korean Journal of Ecology
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• v.23 no.2
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• pp.149-155
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• 2000

The allelopathic effects of Artemisia lavandulaefolia were studied using several test plants and microbes. Aqueous extracts and volatile compounds of A. lavandulaefolia inhibited seed germination, seedling and root growth of the test species such as Achyranthes japonica. Lactuca sativa, Artemisia princeps var. orientalis. Oenothera odorata, Plantago asiatica. Aster yomena, Elsholtzia ciliata, and Raphanus sativus var. hortensis for. acanthiformis. The root growth of test species was more affected than shoot growth by allelochemicals of A. lavandulaefolia. Essential oil of A. lavandulaefolia had antibacterial and antifungal effects. However, the antimicrobial activity of the essential oil was dependent upon the microbial species and concentrations. Callus growth of Oryza sativa, Brassica campestris subsp. napus var. pekinensis and Achyranthes japonica was sensitive by the essential oil of A. lavandulaefolia. Twenty three chemicals were identified from A. lavandulaefolia essential oil by gas chromatography. Primary allelochemicals among them were 1, 8-cineole, 1-$\alpha$-terpineol, $\alpha$-terpinene. camphor, 2-buten-1-ol and azulene. We concluded that aqueous extract and essential oil of A. lavandulaefolia were responsible for allelopathic effects.

• Toxicological Research
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• v.18 no.3
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• pp.233-240
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• 2002

In view of the effect of $\beta_2$-Adrenergic receptors ($\beta_2$-AR) as a risk factor for essential hypertension, we investigated the Fnu4HI and MnlI RFLPs of $\beta_2$ -AR gene in the Korean patients with essential hypertension and normal controls. There were no significant differences in the allele and genotype of these polymorphisms between normotensive and essential hypertensive subjects. In ethnic comparison, the allele frequencies of these three sites contained Nde I RFLP reported the association with essential hypertension in Korean population previously, were very different from those of other ethnic populations studied. The significant linkage disequilibrium was detected only in hypertensive group between Nde I and Fnu4HI sites. The Fnu4HI RFLP was also significantly associated with plasma triglyceride (TG) level. Therefore, our results suggest that the significant association between Fnu4HI variation in the human $\beta_2$-AR gene and plasma TG level may reflect the potential role of human $\beta_2$-AR gene as one of the genetic components for cardiovascular risk.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.10
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• pp.1458-1468
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• 2002

There is increasing interest in exploiting natural products as feed additives to solve problems in animal nutrition and livestock production. Essential oils and saponins are two types of plant secondary compounds that hold promise as natural feed additives for ruminants. This paper describes recent advances in research into these additives. The research has generally concentrated on protein metabolism. Dietary essential oils caused rates of NH$_3$ production from amino acids in ruminal fluid taken from sheep and cattle receiving the oils to decrease, yet proteinase and peptidase activities were unchanged. Hyper-ammonia-producing (HAP) bacteria were the most sensitive of ruminal bacteria to essential oils in pure culture. Essential oils also slowed colonisation and digestion of some feedstuffs. Ruminobacter amylophilus may be a key organism in mediating these effects. Saponin-containing plants and their extracts appear to be useful as a means of suppressing the bacteriolytic activity of rumen ciliate protozoa and thereby enhancing total microbial protein flow from the rumen. The effects of some saponins seems to be transient, which may stem from the hydrolysis of saponins to their corresponding sapogenin aglycones, which are much less toxic to protozoa. Saponins also have selective antibacterial effects which may prove useful in, for example, controlling starch digestion. These studies illustrate that plant secondary compounds, of which essential oils and saponins comprise a small proportion, have great potential as 'natural' manipulators of rumen fermentation, to the potential benefit of the farmer and the environment.

• Advances in Traditional Medicine
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• v.3 no.4
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• pp.187-190
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• 2003

Seven essential oils were tested for in vitro cytotoxicity against the cancerous cell lines A-549, HEp-2 and DLA and normal BRL-3A, NRK-49F and Vero cell lines using standard MTT, SRB and dye exclusion techniques. The A-549 cell line was found to be the most susceptible to all the essential oils. The essential oils of A. nilagirica, A. calamus and O. sanctum were found to be the more active against these cells with mean $CTC_{50}$ values of 17.75, 19.00 and $24.37\;{\mu}g/ml$, respectively. The essential oil of Acorus calamus was found to be the most potent with low $CTC_{50}$ values against the cancerous and comparatively higher $CTC_{50}$ values against the normal cell lines. Artemisia pellens and Pelargonium graveolens oils also showed potent activity. These oils merit further investigation to identify the active principles and nature of the anti tumor activity in animal models.

• The Korean Journal of Mycology
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• v.35 no.1
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• pp.58-60
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• 2007

Root of Asarum sieboldii has been used in chinese medicine. It was been known that the extract of A. sieboldii has medicinal effect such as antifebrile, anodyne, and anti-inflammatory. Antifungal activity of the essential oil extracted from A. sieboldii against various epiderman and opportunistic human pathogenic fungi was investigated. Steam-distillated essential oil demonstrated very high antifungal activity against Aspergillus fumigatus, Aspergillus niger, Cryptococcus neoformans, and Candida albicans. MIC (minimum inhibitory concentration) of the essential oil against C. albicans was 5 ul/ml, indicating that the essential oil may be used as natural preserves for cosmetic and nutritional products.

• Korean Journal of Pharmacognosy
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• v.43 no.4
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• pp.291-296
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• 2012

The dried roots of Angelica decursiva and Peucedanum praeruptorum are registered as the official sources for the drug 'Junho' in Korean Herbal Pharmacopoeia. In this study the essential oils were extracted from the roots of the two plants by steam distillation. Their compositions and the biological activities were compared. As the results of GC and GC-MS analysis, forty one and thirty five compounds were identified in the essential oils of Angelica decursiva and Peucedanum praeruptorum, respectively. Both of the two essential oils contained ${\alpha}$-pinene as the most abundant component. They showed similar significant antioxidant activities in DPPH scavenging assay, and reducing power test. Their dose dependent inhibitions of the nitrosamine formation were identified in experiment using BHA as control. In MTT test of three cancer cell lines, HeLa, MCF-7 and SK-Hep-1, the essential oil from A. decursiva showed stronger activities than that of P. praeruptorum and their common main component, ${\alpha}$-pinene.