• Nutrition Research and Practice
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• 제9권1호
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• pp.17-21
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• 2015

BACKGROUND/OBJECTIVES: In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231 human breast cancer cell line was determined. MATERIALS/METHODS: Cells were cultured with DMSO or with 50, 75, 100 or $250{\mu}g/ml$ of Erythronium japonicum methanol or ethanol extract. RESULTS: Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion of breast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2 and MMP-9 activities were also inhibited by both methanol and ethanol extracts. CONCLUSIONS: Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migration and invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.

• 생약학회지
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• 제23권2호
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• pp.115-116
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• 1992

From the bulbs of Erythronium japonicum Decaisne(Liliaceae), ${\beta}-sitosterol$ and its $3-O-{\beta}-D-glucoside$ together with fatty acids were isolated. All compounds were identified on the basis of spectral data. Campesterol and stigmasterol were also identified as minor components. Paimitic acid was identified as a major component and stearic, oleic, arachidic, behenic, tricosanoic and lignoceric acids were also characterized as minor ones.

• Applied Microscopy
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• 제50권
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• pp.29.1-29.6
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• 2020

Erythronium japonicum (E. japonicum) and Corylopsis coreana Uyeki (C. coreana Uyeki, Korean winter hazel) have been shown to significantly decrease 1,3-dichloro-2-propanol (1,3-DCP)-induced generation of reactive oxygen species and CYP2E1 activity in HuH7, human hepatocytes. In this study, we expanded upon the previous study and investigated the effects of E. japonicum and C. coreana Uyeki extracts on 1,3-DCP-induced liver damage in rats. The pre-treatment of rats with these extracts alleviated a decrease in body weight and reduced 1,3-DCP-induced increase in catalytic activities of hepatic enzymes, such as aspartate aminotransferase and alanine aminotransferase, in the serum. Moreover, treatment with the extracts restored the 1,3-DCP-induced decreases in anti-oxidant enzyme activities, such as the activities of superoxide dismutase and catalase, in the rat liver. Histopathological studies also strongly supported the results of enzyme activities. These results suggest a possibility that the extracts of E. japonicum and C. coreana Uyeki can be a remedy for alleviating 1,3-DCP-induced liver damage in animals.

• Plant Resources
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• 제8권3호
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• pp.263-274
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• 2005

We have studied the effects of dampening papers (Dampened paper, Newspaper, Korean paper, Flower sheet), silica gel (30g, 60g) and temperature ($20^{\circ}C,\;25^{\circ}C$) on color changes of Erythronium japonicum. In the treatment of $20^{\circ}C$, color changes were low in treatments with silica gel rather than in a treatment of dampening papers. In particular, newspaper and Korean paper showed much less changes in colors by the combination treatments with 30g of silica gel. Likewise, in the treatment of $25^{\circ}C$, color changes were low in combination treatments with silica gel rather than in a alone treatment of dampening papers. For the combination treatment with 30g of silica gel, low color changes were shown in the divisions of newspaper and Korean paper, while for the combination treatment with 60g of silica gel, low color changes were shown in the those of Korean paper and dampened paper.

• Natural Product Sciences
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• 제18권3호
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• pp.166-170
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• 2012

The purification of the MeOH extract from the aerial parts of Erythronium japonicum using column chromatography furnished a new megastigmane glucoside, erthrojaponiside (1), together with six known megastigmane derivatives (2 - 7). The structure of the new compound (1) was determined through 1D and 2D NMR spectral data analysis and chemical means. The isolated compounds (1 - 7) were tested for cytotoxicity against four human tumor cells in vitro using a Sulforhodamin B (SRB) bioassay.

• 생약학회지
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• 제24권3호
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• pp.251-254
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• 1993

The anthocyanin isolated from the petals of Erythronium japonicum was elucidated as delphinidin 3-rutinoside on the basis of PPC and UV spectral evidences. To evaluate phytochemical and botanical utilization of this plant, the quantitative analysis of anthocyanin content was carried out by using the visible absorption spectra at 540nm. By the results of our experiment, it was found that the anthocyanin content of petals collected from the summit of Mt. Odae are higher than that of the lowest region. However, there is no significant difference between the anthocyanin contents of the petals collected from the east- and west-side area of the mountain.

• 한국자원식물학회지
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• 제25권3호
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• pp.349-356
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• 2012

The antioxidant potential and enzyme activities in Salicornia herbacea, Corylopsis coreana, Erythronium japonicum, Phragmites communis, Momordica Charantia, Nelumbo nucifera, Salvia plebeia, Portulaca oleracea, Ficus carica, Citrus junos and Cornus officinalis were determined. Their antioxidant activities were measured using DPPH radical scavenging and nitrite scavenging activity. Enzyme activities in investigated plants were evaluated as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The DPPH scavenging rate from 100 to 2500 $mgL^{-1}$ was the highest in the flower of Corylopsis coreana. However, it was not detected in most of the samples at concentration below 100 $mgL^{-1}$. The nitrite scavenging activity according to each kind of resource plants was significantly higher in the stem of Corylopsis coreana and leaf of Nelumbo nucifera. The root extract of Erythronium japonicum had the highest SOD enzyme activity of 94.0% while leaf of Salvia plebeia showed the lowest SOD enzyme activity of 30.4%. The activity of CAT and APX showed higher values in the stem of Corylopsis coreana, root of Erythronium japonicum and root of Phragmites communis in comparison with other plants. The activity of POD showed significantly high values in stem of Corylopsis coreana, Momordica Charantia and pericarp of Citrus junos extracts. The antioxidant enzyme activities differ significantly in different plants. In conclusion, we showed that Corylopsis coreana, Erythronium japonicum Cornus officinalis, and Momordica Charantia had the potent biological activities. Therefore, these plant resources showing antioxidant activity could be good materials for development of source of functional healthy food.

• 생명과학회지
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• 제33권3호
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• pp.227-233
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• 2023

본 연구의 목적은 한국의 금정산 얼레지(Erythronium japonicum) 집단의 지리적 분포에 따른 공간적 분석을 기술한 것이다. 얼레지의 공간적 양상은 Neatest Neighbor Rule, 분산 척도에 의한 다양한 플롯 크기에 따른 집단 응집, 그리고 공간적 상관관계로 분석하였다. 교란된 프롯은 5 m × 5 m 내에서 응집되었다. 얼레지의 대부분 자연 플롯은 산림 군락에서 일정하게 분포되어 있었다. 얼레지의 이웃 패치는 평균 약 7.5 m 에서 10 m 사이에서 이격되어 있었다. 얼레지 자연집단이 인간의 활동으로 교란되었다면 7.5 m~10 m 거리보다 짧은 크기에서 발생한다. 패치 지표(patchiness index, PAI)에 근거한 모리시타 지표(Morisita index, IM)는 금정산 서쪽 사면은 2.5 m × 5 m 플롯이었고 남쪽사면은 5 m × 5 m 이내에 나타났다. 금정산의 서쪽에서 패치 크기가 2.5 m × 5 m 방형구일 때 클러스터는 식물이 가진 종의 특성과 환경적 요인에 의해 결정되었다. 금정산 얼레지 집단은 Moran's I 값의 비교에서 로지스트 회귀로 분석었고, 거리에 의한 격리가 각 개체의 분산에 높은 유의성을 가지고 설명되었다.

• 한국약용작물학회지
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• 제2권1호
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• pp.67-72
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• 1994

어린잎을 식용으로 하고 전초를 차전엽산자고라 하여 강장약 및 진해제로 사용되는 얼레지(Ery-thronium japonicum)의 잎의 성분 연구에 착수하여 phtalate ester인 di -0-n-decylphthalate,fla-vonol인 kaempferol 및 quercetin, 그리고 그들의glucoside인 astragalin및 isoquercitrin의 구조를 동정하였다.

• 한국식품과학회지
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• 제36권6호
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• pp.968-973
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• 2004

나물이나 전분원료로 사용되어 온 얼레지(Erythronium japonicum)의 항암작용을 검색하였다. 얼레지 추출물을 Sarcoma 180으로 복수암을 유발시킨 ICR 마우스에 대하여 경구투여 한 결과 143.7%에 이르는 생명연장효과를 얻었으며, 백혈병계 암세포인 L1210 세포에 대해서는 최고 98.6%의 세포수 감소효과를 얻었다. 아울러 얼레지 추출물의 독성유무를 검색하고자 normal lymphocyte를 분리하여 이 정상세포에 얼레지 추출물을 첨가했을 때 세포수 감소는 거의 일어나지 않았으며, 고농도의 3일간의 긴 배양기간에 의해서도 L1210 세포의 경우 83.2%에 비해 5.8% 정도로 매우 적었다. 이와 같은 결과로부터 얼레지 추출물은 무독성 또는 저독성 항암제로서의 개발 가치가 있을 것으로 사료되었다. 한편 얼레지 추출물의 암세포에 대한 작용 기작으로 일환으로 활성산소인 ${O_2}^-$의 생성량을 측정한 결과 control 값의 최대 약 3.6배까지 크게 증가하였으며, 동시에 ${O_2}^-$ 전환효소인 SOD와 SOD의 종산물인 $H_2O_2$, 분해효소인 GPx도 각각 5배와 3배까지 크게 증가하였다. 따라서 얼레지 추출물은 암세포에서 ${O_2}^-$를 비롯한 활성산소를 유발시키고 이 증가된 활성산소가 암세포의 apoptosis를 야기하는 것으로 간주되었다.