• Nutrition Research and Practice
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• v.9 no.1
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• pp.17-21
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• 2015

BACKGROUND/OBJECTIVES: In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231 human breast cancer cell line was determined. MATERIALS/METHODS: Cells were cultured with DMSO or with 50, 75, 100 or $250{\mu}g/ml$ of Erythronium japonicum methanol or ethanol extract. RESULTS: Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion of breast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2 and MMP-9 activities were also inhibited by both methanol and ethanol extracts. CONCLUSIONS: Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migration and invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.

• Korean Journal of Pharmacognosy
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• v.23 no.2
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• pp.115-116
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• 1992

From the bulbs of Erythronium japonicum Decaisne(Liliaceae), ${\beta}-sitosterol$ and its $3-O-{\beta}-D-glucoside$ together with fatty acids were isolated. All compounds were identified on the basis of spectral data. Campesterol and stigmasterol were also identified as minor components. Paimitic acid was identified as a major component and stearic, oleic, arachidic, behenic, tricosanoic and lignoceric acids were also characterized as minor ones.

• Applied Microscopy
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• v.50
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• pp.29.1-29.6
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• 2020

Erythronium japonicum (E. japonicum) and Corylopsis coreana Uyeki (C. coreana Uyeki, Korean winter hazel) have been shown to significantly decrease 1,3-dichloro-2-propanol (1,3-DCP)-induced generation of reactive oxygen species and CYP2E1 activity in HuH7, human hepatocytes. In this study, we expanded upon the previous study and investigated the effects of E. japonicum and C. coreana Uyeki extracts on 1,3-DCP-induced liver damage in rats. The pre-treatment of rats with these extracts alleviated a decrease in body weight and reduced 1,3-DCP-induced increase in catalytic activities of hepatic enzymes, such as aspartate aminotransferase and alanine aminotransferase, in the serum. Moreover, treatment with the extracts restored the 1,3-DCP-induced decreases in anti-oxidant enzyme activities, such as the activities of superoxide dismutase and catalase, in the rat liver. Histopathological studies also strongly supported the results of enzyme activities. These results suggest a possibility that the extracts of E. japonicum and C. coreana Uyeki can be a remedy for alleviating 1,3-DCP-induced liver damage in animals.

• Plant Resources
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• v.8 no.3
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• pp.263-274
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• 2005

We have studied the effects of dampening papers (Dampened paper, Newspaper, Korean paper, Flower sheet), silica gel (30g, 60g) and temperature ($20^{\circ}C,\;25^{\circ}C$) on color changes of Erythronium japonicum. In the treatment of $20^{\circ}C$, color changes were low in treatments with silica gel rather than in a treatment of dampening papers. In particular, newspaper and Korean paper showed much less changes in colors by the combination treatments with 30g of silica gel. Likewise, in the treatment of $25^{\circ}C$, color changes were low in combination treatments with silica gel rather than in a alone treatment of dampening papers. For the combination treatment with 30g of silica gel, low color changes were shown in the divisions of newspaper and Korean paper, while for the combination treatment with 60g of silica gel, low color changes were shown in the those of Korean paper and dampened paper.

• Natural Product Sciences
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• v.18 no.3
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• pp.166-170
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• 2012

The purification of the MeOH extract from the aerial parts of Erythronium japonicum using column chromatography furnished a new megastigmane glucoside, erthrojaponiside (1), together with six known megastigmane derivatives (2 - 7). The structure of the new compound (1) was determined through 1D and 2D NMR spectral data analysis and chemical means. The isolated compounds (1 - 7) were tested for cytotoxicity against four human tumor cells in vitro using a Sulforhodamin B (SRB) bioassay.

• Korean Journal of Pharmacognosy
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• v.24 no.3
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• pp.251-254
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• 1993

The anthocyanin isolated from the petals of Erythronium japonicum was elucidated as delphinidin 3-rutinoside on the basis of PPC and UV spectral evidences. To evaluate phytochemical and botanical utilization of this plant, the quantitative analysis of anthocyanin content was carried out by using the visible absorption spectra at 540nm. By the results of our experiment, it was found that the anthocyanin content of petals collected from the summit of Mt. Odae are higher than that of the lowest region. However, there is no significant difference between the anthocyanin contents of the petals collected from the east- and west-side area of the mountain.

• Korean Journal of Plant Resources
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• v.25 no.3
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• pp.349-356
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• 2012

The antioxidant potential and enzyme activities in Salicornia herbacea, Corylopsis coreana, Erythronium japonicum, Phragmites communis, Momordica Charantia, Nelumbo nucifera, Salvia plebeia, Portulaca oleracea, Ficus carica, Citrus junos and Cornus officinalis were determined. Their antioxidant activities were measured using DPPH radical scavenging and nitrite scavenging activity. Enzyme activities in investigated plants were evaluated as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The DPPH scavenging rate from 100 to 2500 $mgL^{-1}$ was the highest in the flower of Corylopsis coreana. However, it was not detected in most of the samples at concentration below 100 $mgL^{-1}$. The nitrite scavenging activity according to each kind of resource plants was significantly higher in the stem of Corylopsis coreana and leaf of Nelumbo nucifera. The root extract of Erythronium japonicum had the highest SOD enzyme activity of 94.0% while leaf of Salvia plebeia showed the lowest SOD enzyme activity of 30.4%. The activity of CAT and APX showed higher values in the stem of Corylopsis coreana, root of Erythronium japonicum and root of Phragmites communis in comparison with other plants. The activity of POD showed significantly high values in stem of Corylopsis coreana, Momordica Charantia and pericarp of Citrus junos extracts. The antioxidant enzyme activities differ significantly in different plants. In conclusion, we showed that Corylopsis coreana, Erythronium japonicum Cornus officinalis, and Momordica Charantia had the potent biological activities. Therefore, these plant resources showing antioxidant activity could be good materials for development of source of functional healthy food.

• Journal of Life Science
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• v.33 no.3
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• pp.227-233
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• 2023

The purpose of this paper was to describe a statistical analysis for the spatial distribution of geographical distances of Erythronium japonicum at Mt. Geumjeong in Korea. The spatial pattern of E. japonicum was analyzed according to the nearest neighbor rule, population aggregation under different plot sizes by dispersion indices, and spatial autocorrelation. Most natural plots of E. japonicum were uniformly distributed in the forest community. Disturbed plots were aggregately distributed within 5 m × 5 m of one another. Neighboring patches of E. japonicum were predominantly 7.5~10 m apart on average. If the natural populations of E. japonicum were disturbed by human activities, then the aggregation occurred in a shorter distance than the 7.5~10 m distance scale. The Morisita index (IM) is related to the patchiness index (PAI) that showed the 2.5 m × 5 m plot had an overly steep slope at the west and south areas when the area was smaller than 5 m × 5 m. When the patch size was one 2.5 m × 5 m quadrat at the west distributed area of Mt. Geumjeong, the cluster was determined by both species characteristics and environmental factors. The comparison of Moran's I values to a logistic regression indicated that individuals in E. japonicum populations at Mt. Geumjeong could be explained by isolation by distance.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.67-72
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• 1994

From the leaves of Erythronium japonicum(Liliaceae) fieve aromatic compounds including four flavonoids were isolated and characterized as di -0-n-decyphthalate, kaempferol, quercetin, astragalin and isoquercitrin on the basis of spectroscopic evidences.

• Korean Journal of Food Science and Technology
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• v.36 no.6
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• pp.968-973
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• 2004

Effects of Erythronium japonicum methanol extract on ICR mouse with induced abdominal cancer and L1210 cells were studied. Administration of methanol extract ($10-100\;{\mu}g/20\;g$ body weight) prolonged life by 47.8% and decreased number of L1210 cells with $IC_{50}\;of\;54.6\;{\mu}g/mL$ after 3 days culture, whereas little effect was observed against normal lymphocytes (<6% compared to 83.2% of L1210 cells under the same condition). Increased SOD and GPx enzyme activities, and remarkably augmented generation of ${O_2}^-$ ion in L1210 cells by E. japonicum extract, implied that reactive oxygen species including ${O_2}^-$ ion, might have participated in L1210 cell death