• Nutrition Research and Practice
• /
• 제1권3호
• /
• pp.200-205
• /
• 2007

The effects of taurine on plasma and liver cholesterol, erythrocyte ouabain sensitive Na efflux and platelet aggregation were examined in Sprague Dawley rats fed control or 0.5% cholesterol with 0.2% cholate diet. Plasma and liver levels of total cholesterol were increased significantly (p<0.05) in rats fed cholesterol diet compared to the control, and taurine significantly decreased the elevated plasma level of cholesterol in rats fed cholesterol diet (p<0.05). HDL-cholesterol was decreased in groups fed the cholesterol diet regardless of taurine supplementation and the difference between groups with and without cholesterol was significant (p<0.01). Plasma triglyceride was decreased and liver triglyceride was increased both significantly (p<0.05) in rats fed cholesterol compared to the control. Plasma and liver triglyceride in rats fed taurine was decreased significantly compared to the control (p<0.05). Intracellular Na tended to be lower in rats fed cholesterol or taurine and higher in rats fed cholesterol plus taurine compared to the control. Na efflux through Na-K ATPase and the passive leak of Na was somewhat reduced in rats fed cholesterol or taurine and was augmented in rats fed cholesterol plus taurine compared to the control, which showed a similar trend to the intracellular Na. Taurine supplementation caused a suppression of Na efflux in groups fed control diet and restored the suppressed Na efflux in groups fed cholesterol. Platelet aggregation was significantly decreased in the group fed taurine compared to the control (p<0.05) and the group fed cholesterol plus taurine was also a little lower in aggregation than the group fed cholesterol. Microscopic examination showed that taurine prevented fatty liver in rats fed cholesterol diet. Taurine known for stimulating Na-K ATPase in some cell types rather decreased erythrocyte ouabain sensitive Na-K ATPase in the present study. Taurine had hypolipidemic and hypocholesterolemic effects and inhibited platelet aggregation which may be favorable for prevention of cardiovascular diseases.

• Korean Journal of Acupuncture
• /
• 제24권3호
• /
• pp.91-103
• /
• 2007

Objectives : The purpose of this study was to investigate the usability of live blood analysis on interior and exterior vascular laser irradiation therapy. Methods : We had analyzed the changing forms of the live blood sample with microscope before and after vascular laser irradiation therapy of blood. The live blood analysis was operated on Rouleau of red cell, erythrocyte aggregation, thrombocyte aggregation, uric acid crystals, red crystals, protoplasts. First, we analyzed all patients on each item, then did same thing classified two groups, Interior and exterior. Results : Rouleau of red cell, erythrocyte aggregation, thrombocyte aggregation, uric acid crystals, red crystals, protoplasts were decreased significantly, after interior and exterior aggregation, uric acid crystals. Interior vascular laser irradiation therapy was more effective than interior on Rouleau of red cell, erythrocyte aggregation, thrombocyte aggregation, uric acid crystals. Interior vascular laser irradiation therapy was more effective than exterior on red crystals, protoplasts. Conclusions : This study suggests that live blood analysis has the usability on vascular laser irradiation therapy. Then according to interior and exterior vascular laser irradiation therapy, the result has some different on each item. So it is better that choose the method, interior or exterior, for more effective therapy.

• Korea-Australia Rheology Journal
• /
• 제20권4호
• /
• pp.253-260
• /
• 2008

A method based on transient shear flow dynamics of red cell aggregates was developed to investigate reversible re-aggregation processes with decreasing shear flow. In the microchannel-flow aggregometry, the aggregated red blood cells that are subjected to continuously decreasing shear stress in microchannel flow were measured with the use of a laser-scattering technique. Both the laser-backscattered intensity and pressure were simultaneously measured with respect to time, resulting in shear stress ranging from $0{\sim}35\;Pa$ for a time period of less than 30 seconds. The time dependent recording of the backscattered light intensity (syllectogram) yielded an upward convex curve with a peak point, which reflected the transition threshold of aggregation in the RBC suspensions. Critical-time and critical-shear stress corresponding to the peak point were examined by varying the initial pressure-differential and the micro channel depth, and these results showed good potential for being used as new aggregation indices. In the present study, these newly proposed indices were also validated by differentiating the effect of fibrinogen on RBC aggregation and then these indices were compared to the conventional indices that were measured by a rotational aggregometer.

• 대한한의학회지
• /
• 제25권3호
• /
• pp.99-104
• /
• 2004

Objectives : To study the Live Blood Condition of chronic shoulder measurement. Methods : Twenty-one patients were studied. The observation items of the Live Blood Condition are the form's abnormality and cohesion of red blood cells, the abnormality of hemoglobin and the toxicity in plasma. Results : 1. The form's abnormality and cohesion of red blood cells observed were erythrocyte aggregation (8 persons), Rouleau (3 persons), target cells (7 persons), ovalocytes (3 persons), poikilocytes (2 persons). Double conditions observed were erythrocyte aggregation & target cells (3 persons), erythrocyte aggregation & poikilocytes (1 person), target cells & ovalocytes (l person). 2. The abnormality of hemoglobin and the toxicity in plasma observed were cholesterol crystals (5 persons), atherosclerotic plaque (3 persons), chylous (6 persons). 3. The form normality of red blood was generally observed in one woman. The normality of hemoglobin and the non-toxicity in plasma were generally observed in seven women. Conclusions : According to this study of the Live Blood Condition of women's shoulder measurement, these results suggest that the twenty-one patients evidence the conditions of extravasated blood and phlegm.

• 대한수혈학회지
• /
• 제29권3호
• /
• pp.229-239
• /
• 2018

순환계는 혈액의 흐름이 원활하게 되도록 그 해부학적 구조와 혈류의 물리학적 특성이 연결되어있다. 혈액의 흐름에 영향을 주는 혈액의 물리학적 특성을 혈유변학적 인자(Hemorheologic factors)라 하며, 혈액의 점도(blood viscosity) 및 적혈구 응집도(erythrocyte aggregation)와 같은 혈유변학적 특성은 헤마토크리트(hematocrit; Hct)와 밀접한 관계를 가진다. 헤마토크리트가 높을수록 혈액 점도가 증가하고 적혈구 응집도는 증가하며 순환을 저해하고 조직에 산소 전달 능력을 방해한다. 혈청 페리틴은 과도하게 있을 경우 산화유리기(oxygen free radial)를 통하여 혈관 내피세포(vascular endothelial cell)와 혈구 세포(blood cell)에 산화 손상을 유발하여 심혈관계 손상을 유발한다. 이러한 기전을 근거로 사혈 혹은 헌혈을 심혈관계 질환의 예방 및 치료에 응용하려는 시도가 오랫동안 있었다. 사혈은 의학에 있어 오랜 역사를 가지면서 특히 한방의 치료적 개념으로 최근까지도 사용되고 있으나 그 과학적 근거가 불충분하고 아직까지 논란의 여지도 있어 근거 중심의 의학을 근간으로 하는 의학에서는 도외시되어왔다. 하지만 혈액량이 증가하면 혈유변학적 인자들(hemorheological factors) 역시 악화되면서 대 순환에서 동맥경화의 발생 및 진행에 영향을 주고, 모세순환(microcirculation)을 악화시키는 것으로 알려져 있으며 심뇌혈관 사건에 영향을 주는 것으로 보고되고 있다. 정기적인 헌혈자(regular blood donors)에게 혈유변학적 인자들을 저명하게 호전시키고 과도한 철분의 함량을 줄여서 산화유리기에 의한 혈구 및 내피세포의 산화 손상을 줄임으로써, 순환계내에서 혈액순환을 촉진시킬 수 있다는 증거들이 보고되고 있다. 헌혈의 효과가 심혈관계 질환에 이익이 된다는 확고한 장기적 코호트 연구결과가 도출된다면 절대적으로 헌혈 혈액량이 부족한 현실과 심혈관계 질환의 예후를 호전시킬 수 있다는 측면에서 중요한 역할을 할 수 있다고 생각된다.

• 대한한의학회지
• /
• 제23권2호
• /
• pp.164-179
• /
• 2002

Object: Death rate due to hypertension, atherosclerosis, ischemic heart disease and cerebral infarction induced by Westernized diet and increased average life span is on the rise. Decrease in blood circulation, activation of thrombus generation and intravascular lipid accumulation, cited as the principal causes of the above mentioned diseases in recent studies, result in circulatory disturbance and blood vessel obstruction leading to ischemic cell death of heart, brain and peripheral vessels. Method: We investigated the biochemical changes in microvascular permeability, aggregation of platelet and the intravascular lipid accumulation in induced-diabetic rat using Streptozotocin. We also studied the effects of Woohwangcheongsirn-won after oral administration on blood circulation, platelet function and lipid metabolism. The results are as follows: I. Woohwangcheongsim-won increased blood circulation in microvessels. 2. Woohwangcheongsim-won increased the reduced erythrocyte deformability in diabetes. 3. Woohwangcheongsim-won induced the reduction of contents of 2, 3-DPG, but failed to affect the reduced contents of ATP in erythrocyte in diabetes. 4. Woohwangcheongsim-won reduced the activity of Ca/sup 2+/-ATPase in the membrane of erythrocyte. 5. Woohwangcheongsim-won reduced the platelet aggregation evoked by platelet agglutinin factor. 6. Woohwangcheongsim-won reduced the production of platelet-derived granules. 7. Woohwangcheongsim-won reduced the production of metabolites of arachidonic acid in diabetes, and also reduced the production of increased thromboxane B2. 8. Woohwangcheongsim-won reduced the synthesis of oxidized LDL-cholesterol. In conclusion, Woohwangcheongsim-won enhanced blood circulation in microvesseles, erythrocyte deformability and inhibited the increased platelet aggregation and the synthesis of oxidized LDL-cholesterol in diabetes. Therefore Woohwangcheongsim-won is believed to positively affect blood circulation (J Korean Oriental Med 2002;23(2):164-179)

• 순환기질환의공학회:학술대회논문집
• /
• 순환기질환의공학회 2005년도 제5회 학술대회 초록집
• /
• pp.7-8
• /
• 2005

• Nutritional Sciences
• /
• 제5권4호
• /
• pp.197-202
• /
• 2002

We compared the radical scavenging activity of flavonoids and their antioxidant effects on erythrocyte Na leak, platelet aggregation and TBARS (thiobarbituric acid reactive substance) production, using Sprague Dawley rats. The concentrations of flavonoids needed for scavenging radicals by 50% ($SC_{50}$) in 0.1mM DPPH (2,2 Diphenyl 1-picryl hydrazyl) were: Quercetin, 7.4/$\mu$M; Catechin, 10.6$\mu$M; Morin, 22$\mu$M; Hesperidin, 400uM; and Naringin, 3.95mM. Morin completed its antioxidant activity in 2 minutes, while catechin, hesperidin and naringin had slow but long lasting antioxidant activity. Whole blood platelet aggregation, when incubated with quercetin or catechin, was significantly decreased (P<0.05) compared with the control. Sodium leak in intact erythrocytes was significantly lower when incubated with quercetin, compared with other flavonoids (P<0.05). Morin, hesperidin and naringin somewhat increased Na leak in intact erythrocytes. Sodium leak in erythrocytes treated with phenazine methosulfate (PMS) was increased overall, but was not affected by flavonoids. Intracelluar Na and K were not affected by treatment with PMS. TBARS production in platelet rich plasma (PRP) was significantly lower (P<0.05) than the control when incubated with quercetin or hesperidin. PMS treatment caused an increase in TBARS production regardless of flavonoids. In the present study antioxidant effects of flavonoids were not well correlated with their radical scavenging activities, although quercetin, which showed the strongest radical scavenging activity, had the greatest antioxidant effect.

• The Journal of the Acoustical Society of Korea
• /
• 제25권4E호
• /
• pp.159-165
• /
• 2006

Human, horse and rat bloods in a cylindrical chamber where flow was controlled by a stirring magnet were used for studying red blood cell aggregation. Ultrasound backscattered powers from blood were obtained from the backscattered signals measured by a 5 MHz focused transducer in a pulse-echo setup. The experimental results showed the differences in red blood cell (RBC) aggregation tendency among the three mammalian species with an order of horse > human > rat. The ultrasound backscattered power decreased with stirring speed in human and horse blood, but no variations were observed in rat blood. Sudden flow stoppage led to the slow increase of the backscattered power for human and horse blood. There was no self-aggregation tendency in rat blood. The enveloped echo images showed the spatial and temporal variations of RBC aggregations in the cylindrical chamber. These observations from the different mammalian species may give a better understanding of the mechanism of RBC aggregation.

• 순환기질환의공학회:학술대회논문집
• /
• 순환기질환의공학회 2006년도 제6회 학술대회
• /
• pp.21-23
• /
• 2006