• Mycobiology
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• v.51 no.6
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• pp.445-451
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• 2023

Shiitake mushroom (Lentinula edodes) hold high nutritional and medicinal value as they contain an abundance of health-promoting compounds. However, the effect of long-term postharvest storage on the variation in the levels of health-promoting compounds has not been extensively studied. In this study, we investigated the changes in the levels of phenolic compounds, antioxidants, eritadenine, and ergothioneine in shiitake mushrooms stored at three different temperatures (1, 3, and 5 ℃) for 4 weeks. Compared to mushrooms stored at lower temperatures, those stored at 5 ℃ exhibited a higher level of total phenolics in their pileus after 2 weeks of storage; however, storage at 5 ℃ also increased the deterioration of the fruiting body of these mushrooms. In mushrooms stored at all temperatures, the eritadenine content in the pilei tended to increase up to 2 weeks of storage. In contrast, the ergothioneine content in the pileus decreased during storage, with a significantly lower level detected in mushrooms stored at 5 ℃ for 4 weeks. Together, these results suggest that the mechanisms underlying the accumulation of phenolics and eritadenine may be related to mushroom deterioration during storage. Our findings indicate that the levels of health-promoting compounds in shiitake mushrooms are influenced by storage temperature, suggesting the potential to control adjustments of specific bioactive compounds by regulating storage conditions.

• Journal of Mushroom
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• v.12 no.4
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• pp.258-262
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• 2014

We aim to elucidate suitable mixed color of light during development of fruit body in Pleurotus ostreatus. The four mixed color of LED(Light Emitting Diode), blue and white, green and white, blue and green and green and red LED, were irradiated for formation of fruit-body. First, as effect of mixed color of LED, the properties of fruit body of P. ostreatus in blue and white LED irradiation were showed higher commercial yields than those in control(fluorescent lamp). And the ergothioneine was the highest at the blue and white LED. Second, we could obtain higher commercial yields than those at the $1{\mu}mol/m^2/s$ of light intensity by blue and white LED in P. ostreatus.

• Animal Bioscience
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• v.34 no.10
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• pp.1695-1704
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• 2021

Objective: The aim of this work was to assess the effect of ergothioneine (ESH)-enriched mushroom extract on oxidative stability, volatile compounds, and sensory quality of emulsified sausage. Methods: The ESH content was determined by high performance liquid chromatography. The antioxidant activity of Flammulina velutipes (F. velutipes) extract was determined through radical-scavenging activity of 1,1 diphenyl-2-picryl-hydrazyl, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) and hydroxyl radicals. Four different groups of emulsified sausage were manufactured: control, no antioxidants; BHA, 0.01% butylated hydroxyanisole; EEME, 0.8% ESH-enriched mushroom (F. velutipes) extract; AE, 0.012% authentic ESH, after storage for 14 days (at 4℃), the quality of sausage including oxidative stability (2-thiobarbituric acid reactive substances and protein carbonyls content), volatile compounds and sensory quality were studied. Results: It was demonstrated that adding ESH-enriched F. velutipes extract to sausage could effectively prevent lipid and protein oxidation, and its efficacy was equivalent with 0.01% BHA. During meat processing, the ESH mainly contributed to the antioxidative activity of F. velutipes extract. The flavor and sensory attributes of emulsified sausage were improved through adding ESH-enriched F. velutipes extract. Conclusion: Accordingly, the extract of F. velutipes contained high-level of ESH and could be a good antioxidant candidate for processed meat production.

• The Korean Journal of Food And Nutrition
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• v.27 no.4
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• pp.684-691
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• 2014

This study was carried out to investigate the antioxidant activity and changes in major functional components of fermented Gastrodia elata Blume. Fermented G. elata Blume powder by Phellinus linteus repeated thrice ($3^{rd}$ FGP) showed more DPPH radical scavenging activity than a non-fermented G. elata Blume powder (NFGP), and once fermented G. elata Blume powder ($1^{st}$ FGP) at a concentration of 500 and 1,000 ppm. Free radical scavenging activity of $3^{rd}$ FGP was similar to that of BHA at a dose of 1,000 ppm. Moreover, the ABTS radical scavenging activity of the $3^{rd}$ FGP increased compared to NFGP and $1^{st}$ FGP at a concentration of 31.25 ppm. Total polyphenols and flavonoid contents were increased as fermentation progressed. Ergothioneine content was increased more than 8 times in the $1^{st}$ FGP, 3 times in the $3^{rd}$ FGP, respectively than NFGP. In conclusion, this study indicated that the antioxidant activity and functional component contents of G. elata Blume were increased depending on the fermentation number.

• Journal of Veterinary Science
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• v.24 no.2
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• pp.24.1-24.13
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• 2023

Background: Ergothioneine (EGT) is a natural amino acid derivative in various animal organs and is a bioactive compound recognized as a food and medicine. Objectives: This study examined the effects of EGT supplementation during the in vitro maturation (IVM) period on porcine oocyte maturation and subsequent embryonic development competence after in vitro fertilization (IVF). Methods: Each EGT concentration (0, 10, 50, and 100 μM) was supplemented in the maturation medium during IVM. After IVM, nuclear maturation, intracellular glutathione (GSH), and reactive oxygen species (ROS) levels of oocytes were investigated. In addition, the genes related to cumulus function and antioxidant pathways in oocytes or cumulus cells were investigated. Finally, this study examined whether EGT could affect embryonic development after IVF. Results: After IVM, the EGT supplementation group showed significantly higher intracellular GSH levels and significantly lower intracellular ROS levels than the control group. Moreover, the expression levels of hyaluronan synthase 2 and Connexin 43 were significantly higher in the 10 μM EGT group than in the control group. The expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and NAD(P)H quinone dehydrogenase 1 (NQO1) were significantly higher in the oocytes of the 10 μM EGT group than in the control group. In the assessment of subsequent embryonic development after IVF, the 10 μM EGT treatment group improved the cleavage and blastocyst rate significantly than the control group. Conclusions: Supplementation of EGT improved oocyte maturation and embryonic development by reducing oxidative stress in IVM oocytes.

• Journal of Mushroom
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• v.19 no.3
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• pp.191-199
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• 2021

This study was conducted to improve the useful components and biological activity of Lentinula edodes fermented by lactic acid bacteria (LAB). Three LAB strains (Lactobacillus brevis KCCM 11904, L. plantarum KCCM 354469, and L. fermentum KCCM 12116) were inoculated and used for L. edodes hot water extract (10%, 20%, 30%) fermentation. LAB fermentation of L. edodes hot water extracts decreased pH and thus were more acidic than non-fermented L. edodes hot water extract. β-glucan and ergothioneine contents were increased by L. edodes in a concentration-dependent manner. The ergothioneine and β-glucan contents were highest in fermented with 30% L. edodes hot water extract fermented by L. plantarum and L. brevis (40.48 mg/100 g and 13.94%, respectively). The hepato-protective effect of fermented L. edodes hot water extracts by the three LAB were tested using Sprague-Dawley rat primary hepatocytes. In primary hepatocytes obtained following liver injury induced by acetaminophen, fermented L. edodes hot water extracts by the three LAB showed protective effects, as evident by reduction of the aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase liver markers. The collective results indicate that the fermented L. edodes hot water extracts obtained using LAB are potentially valuable in preventing or treating liver disease.

• Journal of Mushroom
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• v.19 no.4
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• pp.300-309
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• 2021

This study was conducted to develop food and medicinal products containing useful components of Lentinula edodes in Codonopsis lanceolata and Platycodon grandiflorus for use as herbal medicine. We manufactured C. lanceolata (FCLM) and P. grandiflorus (FPLM) extract fermented with L. edodes mycelium. The effect of the two fermented products on proximate composition, free sugar, organic acid, 𝛽-glucan, ergothioneine, ergosterol, and vitamin D₂ levels, and 3T3-L1 preadipocyte cell growth were studied. The proximate composition analysis results showed that the crude fiber and crude fat content in FCLM was higher than that in FPLM, and the crude protein and soluble nitrogen content in FPLM was higher than that in FCLM. Free sugar analysis detected arabinose, glucose, and sucrose in both FCLM and FPLM, and the total free sugar content was high in FPLM. The organic acid content was lower in FCLM and FPLM compared to C. lanceolata and P. grandiflorus before fermentation. The 𝛽-glucan content was higher than that of L. edodes used as a control in both fermented products, FCLM and FPLM. The content of ergothioneine, an antioxidant, was higher in FCLM than in FPLM. Ergosterol content was highest in L. edodes which was used as a control, and the two fermented products showed similar content. Vitamin D₂ was detected only in FCLM and FPLM, and FPLM (0.58±0.01 mg%) showed a higher vitamin D₂ content than FCLM (0.47±0.01). FCLM and FPLM showed a higher level of cell viability for 3T3-L1 pre-adipocytes compared to non-fermented C. lanceolata and P. grandiflorus. In addition, FCLM and FPLM inhibited 3T3-L1 preadipocyte differentiation more than C. lanceolata and P. grandiflorus before fermentation, which may exert an anti-obesity effect.

• The Korea Journal of Herbology
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• v.33 no.1
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• pp.27-35
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• 2018

Objectives : Gastrodiae Rhizoma extract (GE) is possess the various bioactive compounds such as gastrodin, vanilyl alcool and p-hydroxybenzyl alcohol. Various processing methods such as steaming have been widely applied to ease ingestion and enhance the therapeutic effects of plant materials including GE in East-Asia area. The aim of this study was to evaluate the dermal bioactive properties of GE. Methods : First, total phenol, total flavonoid, gastrodin and ergothionein contents of GE were measured. In order to evaluate the dermal bioactive properties of steamed GE compared with not-steamed GE, tyrosinase, collagenase and elastase inhibitory activity were tested. Furthermore, the anti-oxidant activity of GE assessed based on DPPH and ABTS radical scavenging assay. Results : In results, total phenol and total flavonoid contents were increased when 9 times steamed compared to not-steamed GE. Also, GE increased gastrodin contents, in proportion to the number of steaming times and ergothioneine content was abolished in the steaming state. The DPPH radical scavenging activity of GE increased by steaming, but the ABTS radical scavenging activity was not related to the steaming process. In addition, the tyrosinase inhibitory activity was increased as the number of steaming times of GE increased. Collagenase was most inhibited by 4 times steamed GE, and elastase was inhibited by 8 times steamed GE. Conclusion : In conclusion, these results suggest that steamed GE extract has the potential as a cosmetic material which possess anti-oxidant and whitening activities than not steamed GE.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.117-117
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• 2022

표고(Lentinula edodes (Berk.))는 담자균강 주름버섯목 느타리과 잣버섯속에 속하는 식용버섯으로 봄에서 가을에 걸쳐 주로 재배하며, 예부터 식용 및 약용으로 널리 이용되어져 왔다. 우리나라를 비롯하여 일본, 중국 등의 동남아시아 지역뿐만 아니라 최근에는 미국, 캐나다 및 네덜란드 등의 서구 나라에서도 관심이 높으며, 전 세계적으로 중요한 식용 버섯으로써 주목을 받고 있다. 표고는 혈중 콜레스테롤 수준의 감소, 항당뇨, 혈압조절, 면역증강, 간 보호 및 비만 억제 등의 다양한 생리활성이 밝혀져 있어 현대인의 식생활에서 수요가 더욱 증가되고 있는 추세에 있다. 버섯의 생육은 크게 균사의 생장과 자실체의 생육으로 구분되는데, 표고 균사의 배양 기간이 86~123일 정도 소요되며, 자실체의 생육 기간은 10~20일 정도로, 균사의 배양 기간이 훨씬 긴 것으로 나타났다. 버섯의 생육 단계에서 영양, 생식, 생장은 모두 수분으로부터 비롯되며, 생육 조건, 조도, 환기 정도 등에 따라 버섯의 품질은 상당한 차이를 보일 수 있다. 일반적으로 봄에 수확한 표고는 대가 짧고 단단하며 감칠맛이 풍부하며, 가을에 수확한 표고는 대가 길고 육질이 부드럽고 비교적 강수량이 높아 수분함량이 높은 것으로 알려져 있다. 이러한 현상은 표고의 수확 시기에 따라 기후, 온도, 강수량 등의 차이가 이화학적 특성에 큰 영향을 미치는 것으로 생각되어, 본 연구에서는 봄과 가을에 각각 표고를 수확하여 이화학적 특성 및 기능성성분을 비교하였다. 분석결과 가을에 수확한 표고가 봄에 수확한 표고에 비해 수분, β-glucan, ergosterol 그리고 ergothioneine 함량이 유의적으로 높게 나타나, 소재 개발 시에 경제적 경쟁력을 고려했을 때 가을에 수확한 표고 활용이 유리할 것으로 고려된다.

• Korean Journal of Plant Resources
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• v.30 no.1
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• pp.78-87
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• 2017

This study was compared to outdoor field (OF) and rain shelter greenhouse (RSG) according to cultivation type of Gastrodia elata Blume. Also, the effects of yield and quality for mulching materials in RSG were compared. The yield of G. elata was investigated good merchantable quality, and the quality was investigated hardness, chromaticity, weight loss and the contents of the major functional components. The cultivation of RSG was increased the yield, hardness, gastrodin, and vanillyl alcohol compared to OF, and the weight loss, chromaticity, and ergothioneine were almost equal. Rice straw treatment showed higher than those of the control and other treatments on the yield and hardness. As a result, the yield and the quality of RSG, which can control the soil moisture, were better than that of OF. And the lower the soil moisture content according to the mulching material, the harder the surface and the higher the hardness.