Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Chemical components and hepato-protective effect of Lentinula edodes fermented by lactic acid bacteria

표고 유산균 발효물의 성분 및 간기능 보호 효과

  • Im, Seung-Bin (Jangheung Research Institute for Mushroom Industry) ;
  • Kim, Kyung-Je (Jangheung Research Institute for Mushroom Industry) ;
  • Jin, Seong-Woo (Jangheung Research Institute for Mushroom Industry) ;
  • Koh, Young-Woo (Jangheung Research Institute for Mushroom Industry) ;
  • Ha, Neul-I (Jangheung Research Institute for Mushroom Industry) ;
  • Jeong, Hee-Gyeong (Jangheung Research Institute for Mushroom Industry) ;
  • Lee, Jae-Keun (Jangheung Research Institute for Mushroom Industry) ;
  • Yun, Kyeong-Won (Department of Oriental Medicine Resources, Sunchon Nat'l University) ;
  • Seo, Kyoung-Sun (Jangheung Research Institute for Mushroom Industry)
  • 임승빈 (장흥군버섯산업연구원) ;
  • 김경제 (장흥군버섯산업연구원) ;
  • 진성우 (장흥군버섯산업연구원) ;
  • 고영우 (장흥군버섯산업연구원) ;
  • 하늘이 (장흥군버섯산업연구원) ;
  • 정희경 (장흥군버섯산업연구원) ;
  • 이재근 (장흥군버섯산업연구원) ;
  • 윤경원 (순천대학교 바이오한약자원학과) ;
  • 서경순 (장흥군버섯산업연구원)
  • Received : 2021.08.31
  • Accepted : 2021.09.23
  • Published : 2021.09.30
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Abstract

This study was conducted to improve the useful components and biological activity of Lentinula edodes fermented by lactic acid bacteria (LAB). Three LAB strains (Lactobacillus brevis KCCM 11904, L. plantarum KCCM 354469, and L. fermentum KCCM 12116) were inoculated and used for L. edodes hot water extract (10%, 20%, 30%) fermentation. LAB fermentation of L. edodes hot water extracts decreased pH and thus were more acidic than non-fermented L. edodes hot water extract. β-glucan and ergothioneine contents were increased by L. edodes in a concentration-dependent manner. The ergothioneine and β-glucan contents were highest in fermented with 30% L. edodes hot water extract fermented by L. plantarum and L. brevis (40.48 mg/100 g and 13.94%, respectively). The hepato-protective effect of fermented L. edodes hot water extracts by the three LAB were tested using Sprague-Dawley rat primary hepatocytes. In primary hepatocytes obtained following liver injury induced by acetaminophen, fermented L. edodes hot water extracts by the three LAB showed protective effects, as evident by reduction of the aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase liver markers. The collective results indicate that the fermented L. edodes hot water extracts obtained using LAB are potentially valuable in preventing or treating liver disease.

본 연구에서는 기존에 인체에 유익한 효과가 있고 부작용 없이 장기간 안전하게 사용할 수 있으며 간보호 및 간기능 개선에 탁월한 효과가 있는 표고를 추출 및 농축하여 원유에 농도별로 첨가하여, 3종의 유산균을 이용하여 발효하였다. 표고의 농도를 달리하여 유산균을 배양하였을 때 면역관련 물질인 ergothioneine 및 β-glucan의 함량을 측정한 결과 ergothioneine함량은 30% 표고추출농축액에 L. plantarum이 첨가되어 배양된 발효물이 40.48 mg/100 g로 가장 높은 함량을 나타냈다. β-Glucan은 30% 표고추출농축액에 L. brevis가 첨가된 발효물이 13.94%로 가장 높은 함량이 나타났다. 두 면역관련 물질 모두 표고추출농축액 첨가량이 증가할수록 증가하는 경향을 나타냈다. SD rat을 간을 적출하여 일차 간세포로 분리해 발효물의 간보호 효과를 측정하였다. 표고추출농축액과 유산균 발효물을 10, 50, 100, 500 ㎍/mL을 일차 간세포에 처리하여 MTT assay 방법으로 세포독성을 측정한 결과, 모든 농도에서 독성을 보이지 않았다. 또한 10 mM 아세트아미노펜을 처리하여 독성을 유발한 일차 간세포에 시료를 처리하여 AST, ALT, LDH를 측정한 결과, 효소의 농도가 감소하여 간보호 기능에 효과가 있음을 확인하였다. 이중 L. brevis를 첨가하여 배양한 발효물 처리구에서 효소의 농도가 가장 감소한 것을 보았고, 가장 간보호 효과가 뛰어난 것을 확인하였다. 이상의 결과를 토대로 표고추출농축액에 유산균을 첨가하여 발효하였을 때, 기존의 표고보다 면역관련 물질의 함량이 증가하고, 간기능 보호에 더욱 효과가 있음을 입증하였다. 따라서 본 연구결과는 표고 유산균 발효물이 건강기능 소재로서 연구를 위한 기초 연구자료로 활용될 수 있 것으로 생각된다.

Keywords

Acknowledgement

본연구는 중소벤처기업부와 한국산업기술진흥원의 "지역특화산업육성+(R&D-지역주력산업육성, S2915903)" 사업의 지원을 받아 수행된 연구결과임.

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