• The Korean Journal of Food And Nutrition
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• v.29 no.3
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• pp.347-356
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• 2016

This study analyzed the antioxidant properties of Equisetum arvense and its effects on serum factor levels in mice fed a high-fat diet. The aim was to establish a new effective resource for biologically active materials. E. arvense stem and root extracts were obtained using deionized water at $95^{\circ}C$, and 70.5% ethanol at $85^{\circ}C$. These extracts were used to analyze the total phenolic compounds and antioxidant (ABTS, DDPH, and FRAP) activities. The effects of prepared ground samples were evaluated by feeding them to mice. E. arvense extracts showed strong antioxidant effects. The caffeic acid content was highest in the 70.5% ethanol extract of the vegetative stem, as determined by high-performance liquid chromatography. The blood concentrations of insulin and leptin were significantly lower in mice fed a high-fat diet supplemented with extracts of the root, reproductive stem, and vegetative stem of E. arvense than in mice fed only a high-fat diet. These results suggest that the polyphenolic compounds in E. arvense extracts exert various antioxidant effects. The stems and root of E. arvense can lower the blood levels of insulin and leptin, even after consumption of a high-fat diet.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.3
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• pp.139-144
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• 2007

In the previous study, we reported the antioxidative activity of Equisetum arvense extracts. In this study, its inhibitory effect on elastase and components were investigated. Aglycone fractions obtained from the deglycosylation reaction of ethylacetate fraction among the Equisetum arvense extracts, showed 4 bands and 4 peaks in TLC and HPLC experiments, respectively. Four components were identified as luteolin(composition ratio, 19.12%), quercetin(12.87), apigenin(15.81) and kaempferol(52.20). TLC chromatogram of ethylacetate fraction of Equisetum arvense extract revealed 7 bands and HPLC chromatogram showed 8 peaks, which were identified as kaempferol-3,7-O-diglucoside(composition ratio, 15.74%), luteolin-5-O-glucoside(galuteolin, 11.91), apigenin-5-O-glucoside(12.91), kaempferol-3-O-glucoside(astragalin, 27.94), quercetin-glycoside(10.81, structure was not determined), kaempferol-glycoside (12.33, structure was not determined), luteolin(3.72) and apigenin(4.62) in the order of elution time. The inhibitory effect of aglycone fraction on elastase($IC_{50}$, $9.8{\mu}g/mL$) was very high. But ethylacetate fraction(flavonoid glycosides) rarely exhibited the inhibitory activity on elastase. Combined with the previous results of the antioxidative activity of Equisetum arvense extracts, it is concluded that the inhibitory activity on elastase of the aglycone fraction could be applicable to new functional cosmetics for smoothing wrinkles.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.2
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• pp.61-67
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• 2007

In this study, the antioxidative effects of Equisetum arvense extracts were investigated. The free radical (1,1 diphenyl-2-picrylhydrazyl, DPPH) scavenging activity ($FSC_{50}$) of extract/fractions of Equisetum arvense was in the order: 50 % ethanol extract ($182.04{\mu}g/mL$) < ethylacetate fraction ($54.50{\mu}g/mL$) < deglycosylated flavonoid aglycone fraction ($14.13{\mu}g/mL$). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some Equisetum arvense extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol- dependent chemiluminescence assay. The order of ROS scavenging activity was deglycosylated flavonoid aglycone fraction ($OSC_{50}$, $3.54{\mu}g/mL$) < 50 % ethanol extract ($0.80{\mu}g/mL$) < ethylacetate fraction ($0.006{\mu}g/mL$). Ethylacetate fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Equisetum arvense on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethanol extract (50%) suppressed photohemolysis in a concentration dependent manner, particularly deglycosylated aglycone extract exhibited the most prominent celluar protective effect ($\tau_{50}$, 161.10 min at $10{\mu}g/mL$). These results indicate that extract/fractions of Equisetum arvense can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS.

• Applied Biological Chemistry
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• v.49 no.1
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• pp.77-81
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• 2006

This study was carried out to investigate the chemical components, and antioxidative and $anti-{\alpha}-glucosidase$ activities of Equisetum arvense extracts. In Equisetum arvense extracts were composed of 53.20% of crude fiber, 20.42% of crude ash, 15.32% of crude protein and 2.21% of crude fat. Potassium was the most predominant mineral and followed by phosphorus, calcium, magnesium, and sodium. The contents of the unsaturated fatty acids, such as linolenic acid, linoleic acid, and palmitic acid, were higher than those of saturated fatty acids. Seventy percent ethanol extract exhibited antioxidative activity with $IC_{50}$ of $168.1\;{\mu}g/ml$. The Seventy percent methanol extract showed higher ${\alpha}-glucosidase$ inhibitory activity than other solvent extracts.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.20-26
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• 1999

To investigate phytotoxic substances in Equisetum arvense, the aqueous extracts(25 and 8$0^{\circ}C$) or the freeze-drying matter from aqueous extracts($25^{\circ}C$) of E. arvense were tested at different concentrations for biological activities on callus induction and growth, seed germination, seedling growth and antibacterial function. Callus induction and growth of Oryza sativa and Brassica campestris ssp. pekinensis were inhibited by the aqueous extracts at 8$0^{\circ}C$ than at $25^{\circ}C$ and the higher concentrations. Callus growth of four receptor species was inhibited in order of Platycodon grandiflorum, Sesamum indicum, Brassica campestris ssp. pekinensis and Oryza sativa. Seed germination of Ο. sativa, S. indicum, and B. campestris ssp. pekinensis was not affected at low concentration, but it was proportionally inhibited by the higher concentrations. The greatest inhibition of seed germination was 28.3% compared to control, when 2,000$\mu\textrm{g}$/ml of freeze-drying matter was applied to B. campestris ssp. pekinensis. Shoot growth was stimulated by 500$\mu\textrm{g}$/ml of freeze-drying matter, but it was inhibited by the higher concentrations. Root growth was significantly inhibited compare to control at all concentrations. Antibacterial activity of freeze-drying matter didn't showed against Xantomonas oryzae and Eschrichia coli, but a small clear zone was formed by 500$\mu\textrm{g}$ of freeze-drying matter against Erwinia carotovora ssp. carotovora.

• Korean Journal of Pharmacognosy
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• v.29 no.4
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• pp.338-346
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• 1998

In order to elucidate the relationship between anti-HIV-1 enzyme activity and inhibition of HIV-1 replication by natural sources, extracts from some plants using the foods and oriental medicines were tested for inhibitory effects on the viral replication, reverse transcriptase (RT), protease and ${\alpha}-glucosidase$. In the anti-RT test, water extracts of Ficus carica (leaf), Houttuynia cordata (aerial part) and Ixeris tamagawaensis (aerial part) showed more than 79% inhitibion at a concentration of $100\;{\mu}g/ml$. The protease and ${\alpha}-glucosidase-inhibiting$ samples in the screening were water extract of Syringa dilatata (leaf) and methanol extract of Hibiscus syriacus (leaf and stem), which showed more than 40% inhibition at a concentration of $100\;{\mu}g/ml$. In the primary anti-HIV-1 test, water extracts of Equisetum arvense (aerial part), Hibiscus syriacus (leaf), Ixeris tamagawaensis (aerial part) and Pueraira thunbergiana (leaf) showed the potent inhibition against HIV-1 induced cytopathic effects.

• Journal of the East Asian Society of Dietary Life
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• v.8 no.2
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• pp.116-125
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• 1998

This study was designed to find out the anti-cancer effects of two herbs using a folk remedy in Korea-The two herbs were A: Equisetum arvense L. (Seddugi), B: Eunymus alatus Siebold. (Hwasalnamoo) The experiment for the anti-cancer effects were conducted in mice and experimental groups were divided into normal(NO), standard (ST) and two other(A,B) groups for each herb and each of the two experimental groups consisted of three sub-groups(I, II, III) according to three different quantity doses of a folk remedy using five times and ten times the quantity of a commonly used in a folk remedy. The water soluble extracts of two herbs were orally administered to mice during six weeks followed by inoculation of the sarcoma-180 tumor cells into the peritoneal cavity. During experimental periods, the contents of ascites, abdominal organs were observed histopathologically. The results of effects on anticancer were as follows : 1. The survival time was significantly prolonged by the two herbs, especially in groups AII & BII.2. Body weight, circumference of the abdomen and contents of ascites at the time of sacrifice were noted and significantly decreased in groups AI, AII & BII. 3. Anti-cancer effects evaluated by the invasiveness and thickness of the tumor cells were noted in groups AIII & BII.

• YAKHAK HOEJI
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• v.51 no.1
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• pp.51-55
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• 2007

Among pteridophytes, Selaginella involvens Spring and Equisetum orvense L. are used in folk medicine in Eastern Asian countries including Korea. The water extracts from Selaginella involvens spring (SW) and from Equistum arvense L (EW) did not affect viability of human umbilical vein endothelial cells (HUVECs). However, SW dose-dependently inhibited tube formation and migration of HUVECs, whereas EW did not. These results suggest that the water extract from Selaginella involvens Spring may have anti-angiogenic activity.

• Korean Journal of Food Science and Technology
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• v.53 no.6
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• pp.706-714
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• 2021

Chemical scavenging of reactive oxygen species (ROS) is considered a major mechanism of antioxidant effects, but preventing ROS generation can be more efficient in attenuating oxidative damage. In this study, the extracts of plants, Solanum lycopersicum, Ailanthus altissima, Equisetum arvense, and Oenothera biennis, were tested to determine whether their antioxidative effects are driven by the prevention of superoxide generation from mitochondria, a major ROS generator. While all the extracts efficiently attenuated the elevation of ROS levels in human fibroblasts and inflammation-induced mice, those of S. lycopersicum, A. altissima, and O. laciniata only suppressed mitochondrial ROS generation and reduced levels of lipofuscin and lipid peroxidation. Furthermore, the extracts of A. altissima and O. laciniata extended the lifespan of fruit flies. Our results suggest that plant extracts with anti-oxidative effects differ in their ability to prevent ROS generation, which may be associated with the attenuation of oxidative damage in cells and animal tissues.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.6
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• pp.1509-1513
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• 2003

Antifungal activities of the extracts from 26 medicinal plants were investigated utilizing paper-disk diffusion method and (1,3)β-glucan synthase inhibitory assay. (1,3)β-glucan synthase is considered as valuable target in the development of antifungal agents. Among the screened extracts, the ethyl acetate extract of Equisetum arvense, the ethyl acetate extract of Polygonum aviculare, the butanol extract of Crataegus pinnatifida and the n-hexane extract of Saussurea lappa showed significant antifungal activities on Candida albacans in both disk diffusion and enzyme assays.