• 생약학회지
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• 제22권2호
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• pp.85-90
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• 1991

The seasonal variation of two flavonol glycosides, icariin and epimedoside A, in the aerial parts and underground parts of Epimedium koreanum from June through September was investigated. The icariin concentration was decreased with time in both parts. Epimedoside A concentration was fluctuated, being highest in June and lowest in July in the underground parts. In the aerial parts, however, it was almost same in concentration. Determinations were made of the occurrence of two new flavonol glycosides, 2‘-O-rbamnosyl ikarisoside A and 2’-O-rhamnosyl icarisid II in the aerial parts and their changes in concentration were similar to those in the underground parts, being highest in July in both parts.

• 생약학회지
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• 제21권1호
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• pp.56-59
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• 1990

n-Alkanes (n-nonacosane and n-hentriacontane), phytosterols (campesterol and ${\beta}$-sitosterol) and phytosteryl glucosides (${\beta}$-sitosterol 3-O-${\beta}$-D-glucopyranoside and campesterol 3-O-${\beta}$-D-glucopyranoside) were isolated from the underground parts of Epimedium koreanum (Berberidaceae) and characterized by spectral data.

• 생약학회지
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• 제20권1호
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• pp.32-36
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• 1989

Effects of Lycium chinensis, Epimedium koreanum and tuguaconitine which is isolated from Aconitum sibiricum on primary culture chicken embryonic brain cells were studied by microscopic observation and determined of the activity of pyruvate dehydrogenase complex(PDHC). Brain cells were prepared from the brain of 10-day-old chicken embryo and cultured with a medicine consisted of 90% Dulbecco's Modified Eagle Medium(DMEM) and 10% horse serum. It was observed that all substances studied seemed to show the tendency to stimulate the neurite outgrowth of brain cells which were cultured with a deficient medium under microscopic observation. The activity of PDHC in brain cells cultured with a deficient medium was increased by Lysium chinensis and Epimedium koreanum. However, tuguaconitine had not influence on the activity of PDHC.

• Archives of Pharmacal Research
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• 제18권4호
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• pp.289-292
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• 1995

Icariside II, a flavonol glycoside, was isolated from the aerial part of Epimedium Koreanum Nakai by the anti-hepatotoxic acitivity guided fractionation technique employing $CCl_4-in-toxicated$ primary cultured rat hepatocytes as an assay system. Its anti-hepatotoxic activity was evaluated by measuring activity of glutamic pyruvic transaminase released from the $CCl_4-in-toxicated$ primary cultured rat hapatocytes. Icariside II significantly reduced the activity of glutamic pyruvic transaminase released from the $CCl_4-in-toxicated$ primary cultured rat hepatocytes and resulted in 78% recovery of the toxicity at the concentration of $200{\;}\mu\textrm{m}$. The anti-hepatotoxic activity of icariside II on the $CCl_4-in-toxicated$ primary cultured rat hepatocytes was as potent as that of silybin.

• 약학회지
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• 제44권5호
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• pp.455-462
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• 2000

To investigate how the aqueous extracts of Epimedium koreanum Nakai (EK) affects pigmentation of skin, the aqueous extract of EK at various concentrations were incubated with 1 $\times$ 10$^{5}$ melanoma cells per well for 72 h. The morphology and number of cell line were not changed, but the aqueous extract of EK increased the tyrosinase activity and the content of melanin polymer in the cell line. These results indicate that the aqueous extract of EK promotes melanogenesis of Bl6 mouse melanoma cell line.

• 한국식생활문화학회지
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• 제33권1호
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• pp.86-94
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• 2018

This study was designed to evaluate the antioxidant activities and protective effects on PC12 cells of the extract of Epimedium koreanum and its main constituents icariin and icariside I. After screening the seven identified flavonoid glycosides from E. koreanum through DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay, E. koreanum, Icariin and Icariside I exhibited significant effect on radical scavenging activity. E. koreanum, icariin and icariside I were examined using DPPH, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and FRAP (ferric reducing ability power) assay. In all antioxidant assays, E. koreanum, icariin and icariside I showed high radical scavenging activities in a dose-dependent manner. Protective effects against $H_2O_2-induced$ PC12 cells were assessed with MTT assay. The results indicated that cell viability and protection on PC12 cells of icariside I and icariin increased dose dependently. These study results suggest that E. koreanum, icariin and icariside showed high antioxidant capacities and cell protective effects. Icariside I, one of the metabolites of icariin, may be a new and effective flavonoid compound as a functional component.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.314-315
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• 2011

• 한국약용작물학회지
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• 제10권5호
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• pp.340-343
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• 2002

삼지구엽초에 함유되어 있는 icariin 함량을 신속하게 추정하기 위하여 NIRS(근적외선 분광분석기)를 이용한 분석 방법을 검토하였다. HPLC를 이용하여 분석된 삼지구엽초 유전자원 150계통에 대한 이카린 함량치를 NIRS 스펙트럼에 적용시켜 42개의 calibration set 와 26개의 valilion set를 구분하였다. NIRS의 검량식을 몇가지 방법에 의하여 비교분석한 결과 2차미분된 스텍트럼을 MPLS(Modified Partial Least Squares)를 이용한 회귀식에 이용하는 것이 가장 적합하였다. HPLC를 이용한 유전자원들의 이카린 함량은 평균 $0.424%(0.12{\sim}0.67%)$이었으며, NIRS에서 도출된 검량식과의 상관계수는 0.951을 나타내었다. 따라서 삼지구엽초의 이카린 함량은 NIRS를 이용하여 신속 편리하게 분석할 수 있음이 인정되었다.

• 한국약용작물학회지
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• 제10권4호
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• pp.259-262
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• 2002

The efficiency of regeneration of callus and explants from leaf and stem disks of Epimedium koreanum was examined on the MS media containing 2,4-D, NAA, Kinetin, BA and TDZ. Calli were formed on the 2mg/l 2,4-D media at the rate of 32% from leaf discs and 52% from stems. No callus was produced on the media which are containing BA or TDZ alone. The combination of 2,4-D and BA showed the effect on the formation of callus. The combination of 2mg/l 2,4-D and 0.lmg/l BA in the MS media had produced the highest percentage of callus formation, 50% from leaf discs and 40% from stems, respectively. The combination of 2mg/l 2,4-D and 1mg/1 BA in the MS media had affected the formation of callus in the rate of 40% from leaf discs and 25% from stems. The combined plant growth regulators of 2,4-D and BA increased the formation of calli from leaf discs, but single treatment of 2,4-D showed the highest callus formation from stems. Multiple shoots from leaf discs were formed on the media containing NAA, BA, kinetin, and TDZ. The highest number of multiple shoots were obtained 0.1mg/l NAA combined with 1mg/l kinetin. As a result, leaf discs or stems can be used for the mass propagation of Epimedium koreanum, but stem elongation of shoots from calli was not easy.

• 임산에너지
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• 24권2호
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• pp.16-23
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• 2005

삼지구엽초의 각 부분(잎, 줄기, 뿌리)을 MeOH로 추출한 후 농축하고 핵산, 클로로포름, 부탄올, 에틸아세테이트 및 수용성으로 분획하여 동결건조하였다. 각 추출물은 메탄을 수용액 및 에탄올-핵산 혼합액을 사용하여 Sephadex LH-20 칼럼크로마토그래피로 화합물을 분리 한 후 TBA 및 6% 초산에 전개하는 셀룰로오스 박층 크로마토그래피로 단리물질을 확인하였다. 단리물질의 화학적 구조는 $^1H-NMR,\;^{13}C-NMR$ 및 질량 분석으로 결정하였으며 단일화합물로 정제된 물질은 caffeic acid를 비롯하여 flavonoid와 그 배당체 화합물인 icariin, caffeic acid, hyperoside, Ikarisoside A, (+)-catechin이었다. 또한, 각 부분의 클로로포름 분획을 GC/MS (Libraries search)를 이용하여 정성분석을 실시하였다.