• Title/Summary/Keyword: Epidermis

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Experimental Infection of Clonorchis sinensis to Cyprinus curpio nudes (간흡충의 이스라엘잉어(향어)에 대한 감염실험)

  • 이재구;김평길
    • Parasites, Hosts and Diseases
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    • v.21 no.1
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    • pp.11-18
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    • 1983
  • As a part of observation on the suitability as a second intermediate host of Clonorchis sinensis, various infection experiments were tried to Cyprinus carpio nodus. The results obtained were summarized as follows: 1. The cercariae which attempted to contact with Cyprinus carpio nodus in the water were observed under the stereomicroscope. After contact, cercariae began to separate its tails from the bodies at 4 minutes increasingly, and then the numbers ranged to 80% at 13 minutes after the encounter. But very few cercariae could actually invade into the epidermis of the fish. 2. When Cyprinus carpio nudes were exposed to a number of cercariae in the beaker, only a few cercariae could invade through the epidermis. Most of the invaded cercariae were killed before forming the cyst. Also rare encysted cercariae were found to be dead within 48 hours. 3. Cyprinus carpio nudes were reared with Parafossarulus manchouricus shedding numerous cercariae in the indoor aquarium. When observed after 12 days, to determine the susceptibility, no metacercaria was found infected. 4. Cyprinus carpio nudum were reared with Parafossarulus manchouricus shedding numerous cercariae in Kimhae lake for 2 months in summer of 1982, in order to check the natural infection. No metacercaria of Clonorchis sinensis was found from the fish. 5. In the cases of control fish, Pseudoraibora larva, numerous cercariae were inspected on the course of invasion through epidermis of the fish under the stereomicroscope. And many metacercariae were also found from the Ssh whenever they were kept in beaker, indoor aquarium, or the lake for natural infection. The results suggested that Cyprinus carpio kudus might not be proper intermediate host of Clonorchis sinensis.

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The Comparison of Physical & Morphological Damage of Hair According to the Bleaching Methods by the $H_2O_2$ Concentration (탈색단계별 과산화수소 농도에 따른 모발의 물리.형태적 손상정도 비교)

  • Lee, Eung-Kyeung;Hwang, Jung-Deog;Kim, Hye-Jeung
    • Journal of the Korean Society of Fashion and Beauty
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    • v.5 no.1 s.12
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    • pp.71-77
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    • 2007
  • This study attempted to comparative analysis about the physical and morphological damage degrees of hair according to the bleaching methods by $H_2O_2$ concentration. As a result of the changes in the physical characteristics of the hair according to the deceleration operation methods by 3-6-9% and 9% $H_2O_2$ concentration, the intensity of tension considerably decreased but the elongation was increased. And the more the damage of hair, the larger the degree of change. Regarding the method of deceleration operation, the 3-6-9% deceleration operation method was more damaging than the 9% single deceleration operation method. In the change of the characteristics of the form of the hair, the control group of the 5-level deceleration hair generally showed a smooth external appearance and regular and closely overlapped epidermis and undamaged cuticle were observed. But in the 3-6-9% deceleration operation method and 9% single deceleration operation method, damages such as the irregular form of the edge of the epidermis and the unclear boundary between the epidermis occurred.

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Adaptative Characteristics of Some Woody Plants Growing on the Rock Faces ( 1 ) - Morphological Adaptation of Leaves (암반지에 서식하는 식물의 적응적 특성 ( 1 ) - 잎의 형태적 적응)

  • Lim, Joo-Hoon;Zin-Suh Kim
    • The Korean Journal of Ecology
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    • v.14 no.4
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    • pp.371-377
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    • 1991
  • Some morphological characteristics were investigated on the leaves of quercus aliena, hypostomatous species, and lespedeza cyrtobotrya, amphistomatous species, that appeared dominantly on the rock faces in mt.pukhan, mt surak and mt. pulam near seoul. These characteristics were compared with those of normal sites rock faces. All two species growing on the rock faces had thickened leaves with well developed upper epidermis and palisade tissue. Quercus aliena growing on the rock faces showed the leaves with double layer of palisade cells and more regularly arranged spongy parenchyma cells to the lower epidermis. In the case of lespedeza cyrtobotrya, narrower and more lengthened palisade cells and smaller air gaps between the sponge parenchyma cells were observed on the rock faces than those growing in the normal sites. The stomater frequency of the lower epidermis of the tree leaves growing on the rock faces is higher thanthose of normal sites. However, the mean total stomata number of the tree leaves growing on the rock faces are fewer. Most of the morphological characteristics investigated indicate that the plants on the rock faces havesmaller coefficient of variation than those of the normal sites.

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Structure and Cytochemistry of Skin in Spined Loach, Iksookimia longicorpus (Pisces, Cobitidae) (왕종개 Iksookimia longicorpus (Pisces, Cobitidae) 피부 구조와 세포화학적 특징)

  • Park, Jong-Young;Kim, Ik-Soo
    • Korean Journal of Ichthyology
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    • v.12 no.1
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    • pp.25-32
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    • 2000
  • The structure of skin was studied in Iksookimia longicorpus based on the micro-anatomical investigation of skin fragments taken from four regions. The epidermis was distinguished by two types of skin glands, a small mucous cell and a large club cell. The mucous cell was acid sulfomucins (some sialomueins) but the club cell did not give any histochemical tests for mucosubstances. The presence of a well defined lymphatic system with small lymphocytes was established in the stratum germinativum layer of the epidermis. A large number of blood capillaries run very close to each other just below the basement membrane, and a definite area giving AB and PAS positive was present between the basement membrane and scale.

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Studies on the Biological and Chemical Properties of Musty Ginseng Root and its Causal Mechanism (적변삼의 생물.화학적 특성과 그 발생원인에 관하여)

  • 정영륜;오승환
    • Journal of Ginseng Research
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    • v.9 no.1
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    • pp.24-35
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    • 1985
  • Rusty root of ginseng has been known as one of the limiting factors in ginseng production in Korea. An attempt was, therefore, made to elucidate biological and chemical natures of the rusty root, and the redox Potential of the ginseng cultivated soils were measured and compared with diseased and non-diseased soils. Reddish discoloration was most frequently observed on the epidermis of ginseng root and the pigments were accumulated in all epidermal cells of the diseased lesions. The lower the redox potential of the ginseng cultivated soil was, the more severe the rusty root was observed. Fe content in the diseased epidermis was 3 times higher than that of healthy one. Organic acids such as oxalic, malonic, succinic, and citric acids were also higher in the mss root than in the healthy one. Thin layer chromatogram of phenolic acid fractions obtained from the epidermal cells of the rusty root of ginseng exhibited 3 to 4 unidentified substances not found in the healthy root. Also lignification of the epidermal cells and the activity of phenylalanine ammonia lyase were greater in the rusty root than the healthy root. Colony formation and conidia production of F. solani, And mycelial growth and sclerotium formation of Sclerotinia sp. isolated from ginseng root were suppressed in a nutritionally minimal medium supplemented with water extract of rusty ginseng root epidermis. It is, therefore, suggested that rusty root of ginseng is caused by unfavorable rhizosphere environmental stress or stresses resulting abnormal metabolism in the root as a selfdefence mechanism of non-specific resistance responses.

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The Localization of Cytokeratin 19 and Vimentin in Sprague Dawley Albino Rat Skin Tissue

  • Kim, Tae Keun;Kim, Yong Joo;Min, Byoung Hoon;Kim, Soo Jin
    • Applied Microscopy
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    • v.44 no.1
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    • pp.15-20
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    • 2014
  • Cytokeratin 19 (CK19) expressed in epidermis of skin, bulge region of hair follicle, outermost layer of outer root sheath and proximal and distal to bulge. Vimentin is a fibrous protein that localized in cytoplasm of fibroblast and forms cytoskeleton to maintain shape of cell and nucleus. In this study, CK19 and vimentin in skin were confirmed with light, fluorescence and transmission electron microscope. As a result, CK19 was localized epidermis, hair follicles, outer root sheath and nucleus of Merkel's cell. However, vimentin was localized some epidermis, dermis, hypodermis and nucleus of Merkel's cell. The role of CK19 is self-renewal and homeostasis in skin. Also, hair follicle regeneration and hair growth is known to be related. It is supposed that required of structural proteins that make up cytoskeleton is increased. Thereby, expression of CK19 is increased. It is considered that vimentin localized in order to stabilize structure of cell and cytoskeleton of fibroblasts. Also, CK19 and vimentin present in nuclei of Merkel's cell, and to act as a fibrous protein that make up end of a nerve fiber present in Merkel's cell and paracrine function of Merkel's cell.

Histology of the Barbels of Striped Sea Catfish, Plotosus lineatus (Thunberg) (한국산 쓸종개, Plotosus lineatus (Thunberg) 수염의 조직)

  • PARK In-Seok;SEOL Dong-Won;KIM Eun-Mi;KIM Young Ja;LEE Young-don
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.3
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    • pp.158-163
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    • 2005
  • This paper deals with the histology of the barbels of striped sea catfish, Plotosus lineatus (Thunberg). This fish have eight noticeable barbels of two pairs on their maxillary and mandibular. Each barbel is composed of an epidermis, dermis and a central rod of cartilage. The epidermis in the middle part of the maxillary barbel is thicker than those on other parts, and formed of stratified epithelium which contains many cutaneous taste buds and a few small club cells. Number of taste buds increase on the middle and posterior part of each barbel. The dermis consists of loose connective tissue fibers which encloses blood vessels and bundles of nerve fibers. The barbels of this fish can be categorized into stiff and flexible types and are accessory, feeding and sensory structures. Thus we substantiate that they are gustatory receptor organs for this fish.

A Study on methodology of physical Fabrication & reorganization of Epidermis in Space Design - Focused on reorganization of Epidermis, Fabrication - (공간디자인에서 디지털 표피 재 조직화, 물리적 구현 방법 연구 - 표피 재 조직화, 가공 중심으로 -)

  • Park, Jeong-Joo
    • Korean Institute of Interior Design Journal
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    • v.17 no.2
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    • pp.150-161
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    • 2008
  • It requires more close cooperation process and mediator for smooth communication in this industry structure where design and engineers are separated. The database of design integrates separate system and helps connection between organizations. The application category is utilized variously from formation to operation. Architectures addressed in this content as Frank Gehry and Nox are making differentiated design on the base of 3d digital methodology and using it widely from generation to fabrication. Especially they got to be free from the generative limit as it became available to analyse, digital surface organization, and realize the complex system form. Now more integrated and delicate works got to be affordable owing to various kinds of improved CNC, RP(rapid-prototype) machines, and architecture hardwares. With a linkage of software now at their disposal, architects can create a digital model of a building and all of its design elements, and in turn use this 3d information to construct actual building components using machines driven by CNC and other advanced manufacturing techniques. Digital technologies are enabling a direct correlation between what design and construction, thus bringing to the forefront the issue of the significance of information, the production, communication, application, and control of digital information in the industrial system. The central requirement is the clear, reliable, and consistent exchange of information among all parties involved in creating a given project.

Cosmetic Efficacy of Red Pinus densiflora and Its Epidermis Penetration with Polymer Micelle and Cell Penetrating Peptide

  • An, Gyu Min;Park, Su In;Shin, Moon Sam
    • International Journal of Advanced Culture Technology
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    • v.7 no.3
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    • pp.10-24
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    • 2019
  • This study aimed to investigate the effects and epidermis penetration system with polymer micelle of Red Pinus densiflora extract. In the antioxidant test, the total concentration of polyphenol compounds was determined to be $137.5163{\pm}7.70mg/g$ in ethanol extract, $133.956{\pm}1.57mg/g$ in hydrothermal extract. The DPPH radical scavenging effects were $95.29{\pm}0.15%$ in ethanol extract at 1,000 mg/L. Elastase inhibition rates were $100.00{\pm}2.85%$ in ethanol extract at 2,000 mg/L. The antimicrobial effect of the ethanol extraction was higher than that of hydrothermal extractions. In the epidermal permeability experiment, it was confirmed that the permeation of the polymer micelle containing the Red Pinus densiflora's ethanol extract and cell penetrating peptides was remarkable. Here, we confirmed that ethanol extract of Red Pinus densiflora displayed excellent the effects in antioxidant test and epidermis penetration system with polymer micelle. As a result, Red Pinus densiflora extract has potential to be used as a safe and natural cosmetic material in the future.

Fine Structure on the Epidermis of the Scalp of the Head-Irradiated Rats (방사선 조사를 받은 흰쥐 머리피부 표피층의 미세구조)

  • Ko, Jeong-Sik;Kim, Jae-Ho;Yang, Nam-Gil;Ahn, E-Tay;Park, Kyung-Ho;Kim, Jin-Gook
    • Applied Microscopy
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    • v.24 no.2
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    • pp.63-77
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    • 1994
  • This experiment was performed to study the morphological responses of the epidermis of the rat scalp, following X-ray irradiation. Male rats were divided into normal and experimental groups. Rats anesthetized with sodium thiopental, were exposed only on their head areas with a single dose of 3,000rads or 6,000rads, respectively. Radiation was produced by Mitsubishi Linea Accelerator ML-4MV at the speed of 200rads/min. The target distance was 80cm. Animals were sacrificed on six hours, two days and six days following irradiation. By the perfusion fixation through the heart, rats were fixed with 1% glutaraldehyde-1% paraformaldehyde solution. Pieces of the tissue taken from the scalp were refixed in 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide, and embedded within araldite mixture. The sections were cut on a LKB-V ultratome, stained with uranyl acetate and lead citrate, and were observed with JEM 100CX-II electron microscope. The results were as follow; 1. Six hours after exposure to 3,000rads of X-ray. Disrupted intercellular spaces, within which some amorphous materials were filled, disrupted mitochondria, and vacuoles in the keratinocytes were frequently observed, but six days after exposure to 3,000rads of X-ray, Morphology of the keratinocytes was generally restored. 2. Many of the morphological changes were seen on the six days after exposure to 6,000rads of X-ray. 3. Widened intercellular spaces and thickened dense plaques of the desmosomes were frequently observed after exposure to 6,000rads of X-ray. 4. In the experimental groups, the Langerhans and the Merkel cells were damaged, similarly to the keratinocyte. Above results suggest that head irradiation with the dose of 3,000rads temporarily damaged the epidermis of the scalp, though most of the structures recover within six days, whereas with the dose of 6,000rads it severely damaged the epidermis without showing any recovering tendency.

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