• The Plant Pathology Journal
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• v.36 no.5
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• pp.497-502
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• 2020

Barley yellow dwarf virus (BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42℃) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with other cereal-infecting viruses, and the method was 100 times more sensitive than conventional reverse transcription polymerase chain reaction. Furthermore, the assay was validated for the detection of BYDV in both field-collected oat leaves and viruliferous aphids. Thus, the RT-RPA assay developed in the present study represents a simple, rapid, sensitive, and reliable method for detecting BYDV in oats.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1936-1943
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• 2015

The present study developed an efficient and environmentally friendly method for recovering polyhydroxybutyrate (PHB) from Cupriavidus necator. Several non-halogenated solvents were tested and it was found that butyl acetate and ethyl acetate are powerful solvents for the biopolymer. Testing was performed to examine the effects of temperature (25℃ until temperature below solvent boiling points) and heating incubation time (0-60 min) on the two solvents. Butyl acetate had a higher recovery level (96%) and product purity (up to 99%) than ethyl acetate at 103℃ and a heating incubation time of 30 min. Under these conditions, PHB recorded the highest molecular weight of 1.4 × 10⁶ compared with the standard procedure (i.e., recovery using chloroform). The proposed strategy showed that butyl acetate is a good alternative to halogenated solvents such as chloroform for recovery of PHB.

• KSBB Journal
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• v.29 no.4
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• pp.297-302
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• 2014

The autoclaving assisted by an irradiation pretreatment method was developed without toxic chemicals to produce fermentable sugars for their conversion to bioethanol. In the first step, electron beam irradiation (EBI) of rice straw was performed at various doses. The electron beam-irradiated rice straw was then autoclaved with DI water at $120^{\circ}C$ for 1 h. A total sugar yield of 81% was obtained from 300 kGy electron beam-irradiated rice straw after 72 h of enzymatic hydrolysis by Cellulase 1.5L (70 FPU/mL) and Novozyme-188 (40 CbU/mL). Also, the removal of hemicellulose and lignin was 32.0% and 32.5%, respectively. This result indicates that the environmentally-friendly pretreatment method of rice straw by an electron beam irradiation could be applied for bioethanol production in plant.

• Journal of the Korean housing association
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• v.18 no.6
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• pp.57-67
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• 2007

The purpose of this study was to grasp major elements and actual condition of apartment unit remodeling by in-depth interviews on residents and specialists. The interviews had been conducted to interior designers who have a wide experience of apartment remodeling and residents who live in recently remodeled apartment unit. In-depth interview is a kind of qualitative method in which attention to reply of responses with open-access between interviewer and respondents. According to the results, remodeling elements were generally changing of lighting fixtures and finishing materials in the all spaces, integration of balcony into children's room, installation of furniture in kitchen and entrance, changing storage furnitures and equipments in bathroom. Artificial lighting method were selected generally whole lighting. Finishing materials were used ordinary (non environmentally-friendly) wallpaper and adhesion and tempered floor. There were unsatisfied factors after remodeling, these were dissatisfaction of design, construction defects, and stink of reflecting 'sick house'. Also they responded that they should be considering to use environmentally-friendly material at next remodeling.

• KIEAE Journal
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• v.4 no.3
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• pp.137-142
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• 2004

The purpose of this study is to develop the assessment categories, items and detailed criteria for environmental friendly evaluation on the School. The results of this study brings on promoted environmental friendly appreciation, energy and resource conservation, reduction of contaminant, improvement of environment quality in and around buildings. For this purpose, analysis of domestic and foreign environmental friendly architectural citation screening system-BREEAM(1991), LEED 2.0(2001), GBTool(2002), KICTEAC (2000), KOEAM(2000), etc.-was preceded, and through interview, survey and forum with professional group, the developed evaluation items were verified and reappraised. The developed evaluation items were composed of thirty four essential evaluation items and nine bonus items.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.16-16
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• 2023

Metabolomics is the study of global metabolite profiles in a system (cell, tissue, or organism) under a given set of conditions. Metabolomics has its roots in early metabolite profiling studies but is now a rapidly expanding area of scientific research in its own right. In this study, the applications of metabolomics in natural product studies are explored. Ginseng is a well-known herbal medicine and has various pharmacological effects, which include antiaging, anticancer, antifatigue, memory enhancing, immunomodulatory, and stress reducing effects. Metabolomic analysis of organic acids has not been performed for evaluation whether ginseng has been cultivated using conventional or environmental-friendly farming methods. In this study, profiling analysis was conducted for organic acids (OAs) in ginseng roots produced using conventional or environmentfriendly farming methods at five locations in each of five regions. In OA profiles, lactic acid was the most abundant OA in all regions, with the exception for environmentally friendly farmed ginseng in two of the five regions, in which glycolic acid was most abundant OA. OA profiles in all regions showed isocitric acid levels were increased by environment-friendly cultivation, which suggests metabolic differences associated from farming method, and that isocitric acid might be a useful discriminatory biomarker of environmental-friendly and conventional cultivation. The results of the present study suggest metabolomic studies of OAs in ginseng roots might be useful for monitoring whether ginseng has been cultivated using conventional or environmentally friendly farming methods.

• Journal of environmental and Sanitary engineering
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• v.18 no.3 s.49
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• pp.21-26
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• 2003

The generation of waste cable has been continuously increased as a production of electrical and communication media are extended. The current recovery methods, such as mechanical peeling, incineration, solvent extraction and pyrolysis, seems inadequate because they are either hard to apply in some cases or environmentally unacceptable. It has been shown that copper can be effectively separated from the jelly filled type cables using a soybean oil treatment method. As a result, jelly compound is vanished from the wire by soybean oil bath and waste wires are separated copper and PE by the mechanical chipper. This is a more environmentally friendly method than burning, and considerably faster than Stripping.

• Proceedings of the KSR Conference
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• 2001.10a
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• pp.410-415
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• 2001

Conventional Korean tunnel portals require a lot of overburden as, fer static reasons, about 1.5 to 2.0 times the tunnel diameter is needed for the height in order to achieve a sufficient arching effect. Thus, considerable movement of earth and support constructions are required which lead to undesirably large changes of and damage to the environment. With a massively designed pipe roof, tunnels with little overburden can be built. For the effective construction of a pipe roof as an advancing safeguarding method, the following properties are indispensable： stability, insensitivity to settling and drilling accuracy. With the AT casing system a new pipe roof method has been developed which on the one hand entirely combines the properties mentioned last, and which on the other hand permits safe, economical and environmentally friendly construction of tunnels at low overburden heights of 3 to 6 m.

• Journal of Environmental Science International
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• v.16 no.8
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• pp.873-879
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• 2007

For the purpose of development of an environmentally friendly aqueous cleanser, some experimental researches on emulsification of D-limonene were performed. OA series surfactants with different molecular weight were adapted as an emulsifier for preparation of O/W emulsion. Cleaning power of aqueous cleanser was measured by a dipping method adapting abietic acid(AA) as a solubilizate. Besides, drop size and drop size distribution, contact angle and storage stability of the aqueous cleansers were also measured and relationships among them were examined. Decrease in molecular weight of surfactant induced small drop size and contact angie, resulting in high cleaning power of aqueous cleanser. Aqueous cleanser consisted of 3wt.% OA300 and 30wt.% D-limonene showed the highest cleaning power, but displayed unfortunately with low storage stability. The storage stability of the aqueous cleanser with OA300 was significantly enhanced by addition of 0.5wt.% OA600 at the expanse of decrease in cleaning power.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.19 no.2
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• pp.301-305
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• 2010

Cutting procedures where qualities are determined by various demand factors largely influences shipbuilding productivity. Particularly, defects in cutting shapes and cutting surface results in delay for post shipbuilding stages such as in welding and assemblage lines which could become factors for reduced economic viability of the project. Existing cutting procedures utilize fossil fuels such as propane or ethylene as the main fuel component and these methods applied particularly to ship plate cutting gives relatively slow cutting speed and generates large quantities of harmful and sometimes poisonous polluting fumes of which warrants an urgent need to look for alternative cutting methods. Recent introduction of hydrox gas generated by electrically dissociating water into hydrogen and oxygen components to be utilize as an alternative cutting fuel has resulted not just in visible improvement on cutting quality and speed over the existing methods but it has also been welcomed as an environmentally friendly clean fuel source. This paper has been prepared to serve as the basis for accommodating this environmentally friendly hydrox gas cutting method into actual working environment by observing and recording hydrox gas cutting thermal characteristics.