• Korean Journal of Veterinary Service
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• v.35 no.3
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• pp.215-222
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• 2012

Species-specific $TaqMan^{(R)}$ probe-based real-time PCR assays were developed for detection of beef, pork, chicken, duck, goose and turkey. The primer and probe sets used in this study were designed to be complementary to fibroblast growth factor (FGF) for cattle and pig, mitochondrial NADH dehydrogenase (ND) subunit 3 and ND2 for chicken and duck, 12S rRNA for goose and turkey, respectively. As internal positive control we used conserved region in the ribosomal 18S RNA gene to ensure the accuracy of the detection of target DNA by real-time PCR. We confirmed that real-time PCR assays with the primer and probe sets were positive for cattle, pig and chicken intended target animal species with no cross-reactivity with other non-target animal species. Only >50 ng DNA of beef show cross-reactivity in the determination of duck. Using species-specific primer and probe sets, it was possible to detect amounts of 0.1 ng DNA of cattle and pig, 1.0 pg DNA of chicken, duck and turkey, and 0.1 pg DNA of goose for raw samples, respectively. The detection limits were 0.1 ng DNA of cattle, 1.0 ng DNA of pig and 1.0 pg DNA of chicken for DNA mixtures (beef, pork and chicken) extracted from heat-treated ($121^{\circ}C$/5 min) meat samples. In conclusion, it can be suggested that the $TaqMan^{(R)}$ probe-based assay developed in this study might be a rapid and specific method for the identification of meat species in raw or cooked meat products.

• Korean Institute of Interior Design Journal
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• v.22 no.3
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• pp.195-204
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• 2013

Today, the children's museum evolved from place of exhibition for seeing and feeling, to that of exhibition for searching and touching, or so-called the hands-on exhibition. This will allow for grabbing the interest from children and provide an opportunity for learning by an actual experience at this intellectual and benign environment. But in this modern society that constantly undergoes evolution even as we speak, it is not an easy task to bring our children to museums, because they are vastly exposed to various media. Moreover, if the children who visited museums carelessly bypass the specific areas intended for exhibition, the educational purpose of "enhanced learning effect with hands-on experience" is easily underachieved. According to children's visual stimuli, their behavioral charactoristics are appeared to freewill curiosity but they show shorter elapsed time(impermanence), intensity(stubbornness), frequent occurrence(frequentness) and changeable (translatability). So, We need Improvement of visual image affordance through the measurement of stay time. Firstly, five factors are extracted by factor analysis on twenty questions based on visual image factors; Color accessability and Satistaction(factor1), brightness and color harmony(factor2), feeling on harmonization of color and stay time(factor3), simplex & complex of space(factor4), feeling on scale(factor5) Secondly, the following result are derived through a distribution chart on an exhibition room of K-museum. As shown above, this study based on various analyzed aspects proposes the directions of a color image plan to improve stay time in exhibition space of children museums with a hope to support educational goals of experience education-focused children museums.

• Journal of the Korean Institute of Landscape Architecture
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• v.13 no.2
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• pp.37-70
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• 1985

The purpose of this study is to present objective basic data for environmental design by the quantitative analysis of visual quality emboded in physical environment. For this, as for the front garden of high schools, the spatial image was measured by the S.D. Scale Method, Factor Analysis was proceeded by the principal component analysis and the visual preference was investigated by the Paired Comparision Method. The scale values of plain and unpleasant road surface and external appearance of buildings, which are related to emotions of simpleness fell from straightness and stability, were found to be high. But, except for the road surface of Kyunggi High School, scale values of variables explaining the variation of the quality of materials, level of floor and rythm were generally low. For all green spaces, scale values of variables explaining the degree of pleasantness was found to be generally high. And, those explaining tidiness and characteristics of green spaces were not in the same tendency. But, the green spaces of Youngdong High school can be considered to the space with plenty of visual absorption uniqueness were high. As for the correlation between variables, variables for green spaces(12 and 26) and those for overall view of front garden( 1 and 4) revealed high positive correlation. Also, "order - disorder" and "convenient- incovenient" included in road surface variable can be regarded to have the same meaning since the correlation coefficient between them is very high, 0.7045. Image variables including road surface, external appearance of buildings, green spaces and overall view of front garden showed 91.21~61.08% of total variance. Thus, the remains can be considered to be the error valiance or specific variance. In Fctor I, II and III, main components explaining the road surface image of front gardens are order, hardness, texture, color, gradient and rythm. As for the external appearance of b wilding, variables of color, hardness, stability, peculiality and shape revealed high values of factor load. For all variables, communality was drastically high and ellen values and common variance were found to be very high in Factor I. As for the front gardens, variables explaining volume and peculiarity were found to be the main components of Factor I. In Factor II and III, variables of factor load were tidiness, pleasantness.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.E2
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• pp.57-65
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• 2007

In Korea, there is a general lack of information available on air emissions from industry. The reasons for this include the lack of regulatory requirements for emission monitoring, limited information on specific industries, and difficulties in monitoring certain sources. This paper presents the first detailed air pollutant emission factors from composite wood product manufacturing in Korea. This study introduced emission factors for wood-based panels such as plywood, particle board (PB), and medium density fiberboard (MDF). The emission factors of particulate matters (PM) and hazardous air pollutants (HAPs) from MDF were higher than that from other wood products. The concentration of total volatile organic compounds (TVOCs) for hot press from wood-based panels was higher than drying or gluing processes. Emissions data from NPIP were compared to the data from the suggested emission factors in this study and the US EPA's. The data from our emission factors were closer to the observed results than the data using the US EPA's emission factor.

• KIEAE Journal
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• v.7 no.2
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• pp.17-23
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• 2007

While heat island has been recognized as an unique environmental nuisance in cities, the phenomenon tends to be regarded as an inevitable side effect on urbanization. Recently the nature of the heat island has been disclosed and efforts for the remedy have been discussed in many ways. Some pioneering actions have been taken to mitigate the strength of the heat island's intensity in several countries. After studies for the heat island and speculations on current pilot policies of 3 different countries has been done, mitigation policies for heat island has been suggested as followings. 1. Preservation of natural topography is essential because latent energy consumption(evapotranspiration) from the site is the single most important factor to mitigate the energy surplus caused by urban heat island. 2. Because current national zoning ordinance or building law can not effectively control the site specific local environment, heat island policy should be established or employed at local level. 3. Incentives for the mitigation should be adopted on the process of implementation because environment is public concern. 4. Wind can easily dissipate energy surplus which is the major driving force for heat island. Therefore local wind, the direction and intensity should be sustained and sometimes facilitated fully through policies.

• The Journal of Engineering Geology
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• v.25 no.2
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• pp.165-178
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• 2015

Physical and chemical weathering degrades rock, affecting its structural properties and thus the stability of stone buildings or other structures. Confocal laser scan microscopy (CLSM) is used here to observe temporal changes in the surface roughness of rock samples under simulated accelerated weathering. Samples were pressurized to 50, 55, or 70 MPa using a pressure frame, and subjected to freeze/thaw cycling controlled by a thermostat. The temperature was cycled from -20℃ to 40℃ and back. After each 20 cycles, CLSM was used to assess the change in surface roughness, and roughness factors were calculated to quantify the progression of the surface condition over time. Variations in cross-section line-roughness parameters and surface-roughness parameters were analyzed for specific parts of the sample surfaces at 5× and 50× magnification. The result reveals that the highest and lowest values of the roughness factors are changed according to elapsed time. Freezing/thawing at high pressure caused larger changes in the roughness factor than at low pressure.

• Journal of Korean Society on Water Environment
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• v.23 no.1
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• pp.155-160
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• 2007

Constructed wetland has been widely used for the treatment of sewage, stormwater runoff, industrial wastewater, agricultural runoff, acid mine drainage and landfill leachate. For the removal of nitrogen and phosphorus, uptake by plants and adsorption to media material are the major processes, and, therefore, the selection of media with specific adsorption capacity is the critical factor for the optimal design of wetland along with the selection of appropriate plant species. In this study, two media materials (loess bead and mixed media) possessing different adsorption characteristics for ammonium and phosphate were selected, and their adsorption characteristics were evaluated. In addition, the performance of subsurface-flow wetland systems employing these media was evaluated in both batch and continuous flow systems. With LB medium, beter phosphorus removal was observed, while better ammonia removal was obtained with MM medium. In addition, enhanced removal efficiencies were observed in the wetland systems employing both media and aquatic plants, mainly due to the better environment for microbial growth. As a result, appropriate selection or combination of media with respect to the inflow water quality maybe important factors for the successful design and operation of wetland systems.

• Journal of Korea Society of Industrial Information Systems
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• v.16 no.5
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• pp.163-171
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• 2011

In e-commerce environment, security should be considered as an essential factor for success. In this paper, we analyze security requirements for e-commerce system, and it is focused on the practical usage, not theoretical contribution, in the field of e-commerce security. To identify the security requirements being specific to e-commerce environment, the researches related to e-commerce security are surveyed and a phase of Delphi method and Analytic Hierarchy Process(AHP) are used to determine the relative importance of e-commerce security factors. Since researchers and practitioners can have significantly different views because of each different work environment, we divide the professionals into two respondents' group. This survey result can be useful security guidelines in the development of e-commerce service system from the initial system development step to the completion.

• Health Policy and Management
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• v.33 no.2
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• pp.118-128
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• 2023

Background: Personal socio-economic abilities are crucial as it affects health inequalities. These multidimensional inequalities across the regions have been structured and fixed. This study aimed to analyze health vulnerabilities by regional cluster and identify regional health disparities of self-rated health, using nationally representative cross-sectional data. Methods: This study used personal and regional data. Data from the Community Health Survey 2021 were analyzed. K-means cluster analysis was applied to 250 si-gun-gu using administrative regional data. The clusters were based on three areas: physical environment, health-related behaviors and biological factors, and the psychosocial environment through the conceptual framework for action on the social determinants of health. And binary logistic regression analyses were conducted to examine the differences in self-rated health status by the regional clusters, controlling human biology, environment, lifestyle, and healthcare organization factors. Results: The most vulnerable group was group 3, the moderate vulnerable group was group 1, and the least vulnerable group was group 2. The group 2 was more likely to have high self-rated health status than the moderate vulnerable group (odds ratio [OR], 1.023; p<0.001). And the group 3 showed low self-rated health status than the moderate vulnerable group (OR, 0.775; p<0.001). However, the moderate vulnerable group had significantly higher self-rated health status than the most vulnerable group (group 2: OR, 1.023; p<0.001; group 3: OR, 0.775; p<0.001). Conclusion: These results demonstrate that community members' health status is influenced by regional determinants of health and individual levels. And these contribute to understanding the importance of specific and differentiated interventions like locally tailored support programs considering both individual and regional health determinants.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.313-323
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• 2014

Significant alteration in the microbial community can occur across reclamation areas suffering subsidence from mining. A reclamation site undergoing fertilization practices and an adjacent coal-excavated subsidence site (sites A and B, respectively) were examined to characterize the bacterial diversity using 454 high-throughput 16S rDNA sequencing. The dominant taxonomic groups in both the sites were Proteobacteria, Acidobacteria, Bacteroidetes, Betaproteobacteria, Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, and Firmicutes. However, the bacterial communities' abundance, diversity, and composition differed significantly between the sites. Site A presented higher bacterial diversity and more complex community structures than site B. The majority of sequences related to Proteobacteria, Gemmatimonadetes, Chloroflexi, Nitrospirae, Firmicutes, Betaproteobacteria, Deltaproteobacteria, and Anaerolineae were from site A; whereas those related to Actinobacteria, Planctomycetes, Bacteroidetes, Verrucomicrobia, Gammaproteobacteria, Nitriliruptoria, Alphaproteobacteria, and Phycisphaerae originated from site B. The distribution of some bacterial groups and subgroups in the two sites correlated with soil properties and vegetation due to reclamation practice. Site A exhibited enriched bacterial community, soil organic matter (SOM), and total nitrogen (TN), suggesting the presence of relatively diverse microorganisms. SOM and TN were important factors shaping the underlying microbial communities. Furthermore, the specific plant functional group (legumes) was also an important factor influencing soil microbial community composition. Thus, the effectiveness of 454 pyrosequencing in analyzing soil bacterial diversity was validated and an association between land ecological system restoration, mostly mediated by microbial communities, and an improvement in soil properties in coal-mining reclamation areas was suggested.