• Journal of Life Science
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• v.27 no.3
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• pp.351-354
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• 2017

Cowpea (Vigna unguiculata (L.) Walp.) is recognized as a potential source of protein and other nutrients. The genus Vigna includes 100 wild species of plants. Especially, Vigna unguiculata includes annual cowpeas (ssp. unguiculata) and ten wild perennial subspecies. DNA sequence of internal transcribed spacer (ITS) region was determined for Vigna sinensis, one of native plant, which was found in recent but thought to have gone extinct in Korea. The seeds of Vigna sinensis used in this study were donated from Dong-Young Jo. The DNA sequence of ITS-5.8S-ITS2 for Vigna sinensis obtained from this study was deposited as Vigna sinensis AY195581 on GenBank of NCBI (National Center for Biotechnology Information). We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. DNA similarities among subspecies including Vigna unguiculata showed the range 98 to 100% in sequence-based phylogenetic analysis using total 507 base pairs of ITS1, 5.8S and ITS2. Vigna unguiculata and subspecies were grouped independently as one cluster from other Vigna species used in the phylogenetic analysis. In this study, based on the phylogenetic analysis using the ITS1-5.8S-ITS2 sequence of Vigna sinensis, it may be concluded to be classified to one of Vigna unguiculata substrains.

• Clean Technology
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• v.20 no.3
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• pp.205-211
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• 2014

Biomass-based plastics containing the biomass content higher than 25 wt% have been considered as environment-friendly materials due to their effects on the reduction in the $CO_2$ emission and petroleum consumption as well as biodegradability after use. This article described the effect of the additions of oxo-biodegradable additive, 4 kinds of plant biomass, unsaturated fatty acid, citric acid in the properties of polyethylene films. Bio films were prepared using a variety of biomasses and tested for feasibility as a food packaging film. Mechanical properties such as tensile strength and percent elongation at break were evaluated. Husk biomasses from such as corn, soybean, rice, and wheat were pulverized using air classifying mill (ACM) and four different types of packaging films with thickness of $50{\mu}m$ were prepared using the pulverized biomass and low density polyethylene/linear low density polyethylene. The packaging film with wheat husk biomass was found to have greater mechanical properties of elongation and tensile strength than the other samples. Biodegradability of bio film was measured to be 51.5% compared to cellulose.

• Journal of Korean Society of Rural Planning
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• v.17 no.4
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• pp.25-38
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• 2011

본 연구는 방글라데시의 친환경농업 실태와 농업인들의 인식을 바탕으로 친환경농업 개발에 대한 정부의 역할과 정책적 대안을 모색하고자 하였다. 연구 자료는 방글라데시 탕가일 지역의 농업인을 대상으로 유기농가 100가구와 일반농가 100가구를 무작위로 추출하여 설문조사를 통하여 수집되었다. 인구 과밀의 방글라데시 정부는 식량안보를 위하여 지력 저하 및 환경악화를 방지하고자 많은 노력을 기울이고 있다. 즉 지속 가능한 농업개발을 위하여 통합해충관리(IPM), 통합작물 관리(ICM)를 비롯하여 토양의 비옥도 개선, 페로몬트랩이나 자연 천적을 이용한 해충방제 등 다양한 프로그램을 운영하고 있다. 농업인들의 의견 조사에 따르면 친환경농업에 대한 정부의 역할은 긍정적으로 평가하고 있으나 정책이나 관련 프로그램은 아직 부족한 것으로 나타났다. 일반농가인 경우 극히 소수 농가만이 친환경농업을 실행하고 있는데, 이는 관련기술에 대한 지식의 부족과 유기농 비료 등의 자재 부족이 친환경농업을 실행하는데 가장 큰 어려움이라 지적하고 있다. 일반농가의 39%는 유기농업 의사를 보이고 있지만 마케팅시설의 부족과 농사 실패에 대한 두려움 등을 유기농으로 전환하지 못하는 주요 이유로 들고 있다. 일반농가의 일부(17%)만이 정부의 농업교육 프로그램임인 통합해충관리(IPM)과정을 이수하고 있다. 정부의 유기농 정책은 거의 전무하며 NGO들의 지원에 의하여 꾸준하게 진행되고 있을 뿐이다. 따라서 농업인들은 통합해충관리(IPM), 유기농 퇴비, 페로몬트랩이나 자연 천적 등 매우 다양한 농민교육프로그램들을 정부 주도적으로 실시하여야 하며 친환경농업정책을 보다 명확하게 규정하기를 요구하고 있다. 비교적 젊고 교육수준이 높은 소규모 농업인들이 유기농에 적극적임을 알 수 있다. 유기농의 평균 농지규모는 0.246ha, 유기농 평균 경력은 7.63년, 일반농가대비 농가수입은 11.4% 높게 나타났고 비교적 소농들이 높은 수익률을 보이고 있다. 소비자의 신뢰, 마케팅시설, 생산자단체나 조직의 부족 등이 유기농가들의 가장 큰 애로점이 되고 있고, 유기농산품의 표준화를 위하여 정부의 인증제도와 인증기관 설립이 필요함을 지적하고 있다. 유기농을 보다 활성화하기 위해서는 정부 주도하에 마케팅을 비롯하여 교육, 수출 및 각종 장려책 등이 마련되어야 할 것이다.

• Journal of Mushroom
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• v.16 no.3
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• pp.186-191
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• 2018

In order to breed a new Pleurotus eryngii cultivar with a large pileus and convex shape, which are favorite traits of customers from Europe and North America, single crosses between monokaryotic mycelia derived from basidiospores of KNR2555 were performed to yield the new cultivar $2{\times}12$ chosen by cap shape (convex), diameter of pileus (60.7 mm), and quality (4.9) in a preliminary cultivation. The strain was named Gat_aeryni and was cultivated on a large scale for comparison with Kenneutari 2 ho at the GNARES and at mushroom farms. The yield of Gat_aeryni (71.7 g) was not significantly different from that of Kenneutari 2 ho (71.4 g), and the quality of the new cultivar was 6.8, which was not significantly different from that of a reference cultivar (6.5). Days for harvest, length, and diameter of the pileus in the two cultivars were statistically different by an independent t-test (P < 0.001, P < 0.05, P < 0.001 and P < 0.05 respectively). The new and reference cultivars were discriminated by PCR reactions with a primer set (URP1 and URP10) and simultaneous cultivation.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.295-310
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• 2017

This study investigated the growth of five phytopathogenic fungi including Colletotrichum gloeosporioides, Diaporthe citri, Phytophthora capsici and others according to different concentrations of water extract in order to provide reference data for developing environment-friendly agricultural materials using five native Fagaceae species including Quercus acuta, Quercus salicina, Quercus glauca, Quercus gilva and Castanopsis cuspidata var. sieboldii. As the concentration of aqueous extracts of Fagaceae increased according to donor plants, the mycelial growth of phytopathogens showed a decreasing tendency. Differences were found in the degree of inhibition according to types of donor plants and pathogenic fungi. Diaporthe citri, Phytophthora capsici, Pythium graminicola on the water extract of Castanopsis cuspidata var. sieboldii inhibited mycelial growth by 84% in 25% of the treatment group and by 87% in more than half of the treatment group. The water extract of Quercus acuta was found to have no inhibitory effect against the mycelial growth of Diaporthe citri. The aqueous extracts of Quercus salicina, Quercus glauca and Quercus gilva insignificantly inhibited mycelial growth by approximately 15%. The total phenolic content of receptor plants exhibiting antifungal activity was highest in Castanopsis cuspidata var. sieboldii with a content of 22.32 mg/g phenols, followed by Quercus salicina with 8.32 mg/g, Quercus glauca with 6.83 mg/g, Quercus gilva with 5.95 mg/g, and Quercus acuta with 5.24 mg/g. The aqueous extracts of Castanopsis cuspidata var. sieboldii among the five Five Fagaceae Plants of were the most effective antifungal activity.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.445-460
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• 2017

This study was carried out for three years from 2013 to 2015 to investigate the actual condition of pest control of domestic persimmon organically cultivated farmers, to select environment - friendly pest control materials for major pests, and to conduct field test of established control system. The main cultivated cultivar of the reader farmer of organic cultivation was "Buyou" and sprayed organic agricultural material 9~17 times per year for pest control. The most harmful pests were anthracnose, bugs, persimmon fruit worm, and scales, etc. The control materials used were organic lime sulfur mixture, red clay sulfur, emulsifier, bordeaux solution, and self-made plant extracts using garlic, ginkgo nut and pine tree. The effective materials for controlling anthrax were lime sulfur mixture and red clay sulfur. Garlic oil + citronella emulsion, shrubby sophora seed extract + tea extract was effective to control Riptortus clavatus and Euproctis subflava. When installed 60 ea per 10a of mating disruption trap in a sweet orchard, the fruit setting rate was improved by 30%. The results of field test of control system to control 10 times a year in organically grown persimmons were able to harvest fruit of 70.7%. However, since any kinds of pesticides can not be sprayed during the harvest season in September and October, the damaged by bugs did not decreased, and further research is needed.

• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.554-567
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• 2019

Recently, concerns regarding the environmental impact due to nutrient input in croplands have increased. Therefore, the government is promoting the introduction of a nutrient management system in croplands to solve the problem of excessive nutrient input. This study was carried out to establish nutrient indicators in regional croplands to facilitate the introduction of the national nutrient management system in Korea. The nutrient load and balance indicators for nitrogen and phosphorus were analyzed for nine provinces (Gang-won, Gyeong-gi, Chung-buk, Chung-nam, Jeon-buk, Jeon-nam, Gyeong-buk, Gyeong-nam, and Jeju). In the correlation analysis between the nutrient load and nutrient balance, the correlation coefficient (r) for nitrogen was 0.2504, which was not statistically significant at the 5% significance level. However, the correlation coefficient for phosphorus was 0.7375, which was statistically significant at the 5% significance level. In the nutrient management index, phosphorus showed mutual compatibility between the nutrient load and the nutrient balance indicators, but nitrogen showed no mutual compatibility between the nutrient load and the nutrient balance indicators. Therefore, utilization of the nutrient balance indicator, reflecting the characteristics of the agricultural environment, was more reasonable as a nutrient management index for regional nutrient management.

• Journal of Life Science
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• v.26 no.10
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• pp.1202-1206
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• 2016

Unicellular cyanobacterial strains of Subsections I and II and filamentous cyanobacterial strains of Subsection III have been shown to be polyphyletic, heterocystous strains of Subsections IV and V, both of which were previously reported to be monophyletic. In this study, the small subunit ribosomal RNA (16S rRNA) sequences of 13 strains of cyanobacteria - one strain, Oscillatoria nigro-viridis PCC7112, of the Subsection III, 6 strains including genus Anabaena, Nostoc, Tolypothrix, Calothrix and Scytonema of the Subsection IV, and 6 strains including genus Hapalosiphon, Fischerella and Chlorogloeopsis of the Subsection V - were determined. The phylogenetic analysis of cyanobacteria was carried out using the 16S rRNA sequences. The results of the phylogenetic analyses of 16S rRNA sequences, based on Neighbour-joining, maximum-parsimony, and maximum-likelihood methods, indicated that the members of Subsection IV were not monophyletic but polyphyletic. In addition, the phylogenetic results strongly indicated that the genus Scytonema in Subsection IV could be a common ancestor of heterocystous cyanobacteria in Subsection IV and V. Furthermore, the phylogenetic analyses revealed that the genus Anabaena could be phylogenetically diverse and that cyanobacterial strains in Subsection IV might be polyphyletic, whereas those in Subsection V could be monophyletic, as reported before. The results for the genus Anabaena indicate that it should be reclassified.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.459-464
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• 2016

An outbreak of the migratory locust, Locusta migratoria, in the environment-friendly reclaimed plantations of forage crops in Sanyimyeon, Haenam-gun, Jellanam-do, Korea in August 2014 caused severe damages to various crops. Owing to its first occurrence in the Korean history, the causes underlying the outbreak and phase-transition of the migratory locust were not known. It is critical to establish the genetic relationship of the migratory locust in Sanyimyeon, Haenam-gun with the other previously reported strains in the world in order to understand the mechanisms responsible for its outbreak. The gene sequences of the 16S ribosomal RNA (rRNA) and displacement-loop (D-loop) of the mitochondria of various regional species of the migratory locust were used to perform the phylogenetic analysis. Our results suggested that the migratory locusts in Sanyimyeon, Haenam-gun are closely related with the Eurasian strains of the northern lineage. In future, these two mitochondrial genes can be used for elucidating the genetic population structures in migratory locusts in various regions. In addition, the sequence information of these genes can be used to enhance our understanding of the genetic basis of the outbreak of migratory locusts.

• Journal of Environmental Policy
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• v.13 no.3
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• pp.109-133
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• 2014

With a growing concern about climate change and green house gases mitigation, carbon labeling policy has been launched in several countries as an environmental policy which connects low carbon production to low carbon consumption. This research aims to propose a model that explains consumers' attitude and brand loyalty toward carbon labeling products. This model specifies the consumer's psychological processes by which consumer values, such as autonomy and environmental values, affect carbon labeling product and corporate images and finally form brand loyalty toward carbon labeling products. Panel data were collected in two separate surveys and analyzed using a structural equation technique. Results are summarized as follows. First, consumers' autonomy value(AV) positively affects locus of control(LC) and corporate image(CI). Second, consumers' environmental value(EV) positively influences perceived consumer effectiveness(PCE), which in turn has a negative effect on perceived barriers(PB). Perceived barriers finally affect product image(PI) negatively. Third, both corporate image and product image have causal relationships with brand loyalty. Our results suggest that carbon labeling policy contributes not only to the reduction of greenhouse gases but also to the increase of consumers' attitude and brand loyalty toward carbon labeling products. This research also provides governments with directions for efficient environmental policy and firms with guidance on effective marketing strategies about carbon labeling.