• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1836-1840
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• 2008

Bacteriocins are antimicrobial peptides that are produced by bacteria and toxic to bacterial strains closely related to the producer strain. It has previously been reported that Bacillus thuringiensis strain NEB17 and Bacillus thuringiensis subsp. kurstaki BUPM4 produce the bacteriocins thuricin 17 (3,162 Da) and bacthuricin F4 (3,160.05 Da), respectively. Here, we demonstrate that these bacteriocins have functional similarities and show a similar spectrum of antimicrobial activities against indicator strains. We also studied the effects of sterilization methods on the recovery and biological activities of these bacteriocins. They were completely degraded by autoclaving and the two were similarly affected by the tested filter membranes. Polyvinylidene fluoride (PVDF), polyestersulfone (PES), and cellulose acetate (CA) are suitable for filter sterilization of these bacteriocins. The two bacteriocins were stable across a range of storage conditions. These data will facilitate their utilization in food preservation or agricultural applications.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.2
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• pp.144-150
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• 2010

The agricultural techniques of environmental friendly using the green manure crops have been required recently more to have the safety agricultural products and to reduce the use of fertilizers and agricultural chemicals. The utilization of green manure crops related closely to cropping system would be very important. The purposes of this study are both to investigate the effects of green manure crops of hairy vetch, legume, and rye, gramineae, and to compare the effects between them in rice cultivation. The hairy vetch and rye were treated as green manure crops into paddy soil at 10 days before the rice transplanting. The plant height was increased gradually from the maximum tillering to the heading growth stage, showing that it was the highest with treatment of conventional cultivation in 2007 and the highest with hairy vetch in 2008. The number of tillers was higher with treatment of hairy vetch and hairy vetch+rye than those with the conventional cultivation. Dry weight was also higher with hairy vetch than that with the conventional, while it was lower clearly in rye than those in hairy vetch and conventional. According to the high tilller number and spikelet number per panicle out of the yield components relatively, the yield of rough rice was increased to about 6% and 8% in 2007 and 2008, respectively, comparing with the yield in the conventional cultivation. Based on these results, the hairy vetch would be a good green manure crop in rice cultivation.

• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.180-185
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• 2012

The objective of this study was carried out to elucidate the effect of LEDs (light emitting diodes) irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Rollo Rosa'). In morphological changes of leaves, shoot elongation and hypocotyl length showed poor growth in red light irradiation, while the red + blue light irradiation induced shorter plant height and much greater leaf numbers resulting in increased fresh weight. In change of the Hunter's color and SPAD values, lettuce seedlings grown under in red + blue and fluorescent light irradiation had a higher $a^*$ value, otherwise SPAD values were not changed in these light irradiations. Interestingly, relative chlorophyll contents showed 1.8 times increased redness in the treatment of red + blue light irradiation. Inorganic element (N, Ca, Mg, Mn, and Fe) and ascorbic acid contents were increased in lettuce plants grown under LEDs light irradiation compared to those of lettuce grown under the fluorescent light which showed higher P and Mn contents. In conclusion, it is considered that red + blue light irradiation which stimulates growth and higher nutrient uptake in leaf lettuce could be employed in containers equipped with LEDs.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.533-541
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• 2017

Agricultural landscape has many ecosystem service functions. However, the development of the horticulture complex has no consideration for environmental conservation. Therefore, we analyzed the priorities of ecosystem service functions required for the composition. The study was conducted in three stages. As a result of the first survey, 17 functions were selected to be improved. In the second survey, 12 functions were selected excluding 5 functions. Finally, 1. Measures for water purification, 2. Groundwater recharge plan, 3. Surface water storage space, 4. Flood control measures, 5. Vegetation diversity space, 6. Carbon emission reduction plan, 7. Aquatic insect habitat space, 8. Amphibian reptiles 9. Landscape and Waste Management, 10. Bird Species Space, 11. Heat Island Mitigation Plan, 12. Experience / Ecological Education Plan. We proposed the structure, capacity, flow rate, arrangement and form of the water treatment facility to improve water quality by improving the function. We proposed a reservoir space of 7-10% for groundwater recharge. The development of reservoir and storage facilities suitable for the Korean situation is suggested for the surface water storage and flood control measures. And proposed to secure a green space for the climate cycle. Proposed habitat and nutrient discharge management for biodiversity. We propose green area development and wetland development to improve the landscape, and put into the facilities for experiential education. The results of the research can be utilized for the development and improvement of the horticultural complex.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.10 no.1
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• pp.8-15
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• 2011

ATV(All Terrain Vehicle) has been developed to be used for agricultural fields on unpaved roads but now it is mostly used to transport a short distance and play leisure sports at mountains. Recently, main concerns on the automobile industry is developing not only a car safe, high fuel efficient and friendly related with environment but also two-wheeled vehicles. In order to maintain high fuel efficiency and safety of two-wheeled vehicle, it is essential that ATV should be set up differential gear. But we worried for ATV which had been set up differential gear not to run on unpaved roads. Therefore, it is necessary to develop LSD(Limited Slip Differential) that maintains existing advantages of ATV and ensures ATV to travel safely on roads. Now LSD equipped cars have a complicated structure, so setting up on ATV is unsuitable. Therefore, in this study, we have developed simple, small and well operating LSD.

• Mycobiology
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• v.28 no.4
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• pp.180-184
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• 2000

In order to find an environment-friendly method to suppress astragal stem rot caused by the isolates of Rhizoctonia solani AG 1 and AG 4, we tested an antagonistic fungus Gliocladium virens G1 was evaluated as a biocontrol agent and estimated inorganic compounds and organic materials were tested for their effect of the disease suppression. G. virens G1 effectively inhibited mycelial growth in a dual culture and caused mycelial lysis in the culture filtrate. No adverse effect was observed when examined for seed germination and seedling growth. Promoted seedling growth was observed with the seed treatment. Seeds of astragal plant were germinated higher in the sterile soil than the natural soil. Of 14 inorganics tested, alum, aluminum sulfate and calcium oxide significantly suppressed the mycelial growth and sclerotial germination. Milled pine bark and oak sawdust also suppressed the mycelial growth. Soil amended with 1% of G. virens G1 composted with pine bark (w/v) significantly controlled astragal stem rot in the glasshouse experiments.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.87.1-87
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• 2003

Powdery mildew on strawberry plants, caused by Spherotheca aphanis (Wallr.) U. Braun var. aphanis, is the most serious disease for strawberry production. There is a demand to develop the substitutes for chemicals which are more environment friendly materials. Control of powdery mildew was evaluated on Akihime, Sachinoka, Dochiodome, Noyho and Redpearl varieties grown in the greenhouse. Applications of 1％, 0.5％, and 0.25％ of sodium bicarbonate (NaHCO3Nyoho in greenhouse experiments. Non-phytotoxicity was revealed on the leaves and fruits of strawberry at these concentrations. This$.$result indicates that a mixture of sodium bicarbonate and tween 20 is a useful substitute for fungicides to control powdery mildew of strawberry.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.2
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• pp.73-78
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• 2023

Currently, Hyphantira cunea Drury, the fall webworms (FWW), is one of the most severe insect pest for various landscaping trees and mulberry trees. In this study, we investigated whether FWW might be managed by Sodium saccharin (SAC) which is a food additive and not toxic to humans and environment. We found that FWW larvae treated with various concentrations of SAC showed dose-dependent delayed development and low survival rates. The lethal-concentration 50% of FWW larvae to SAC was 0.03 M. We also confirmed that SAC can be used to control FWW larvae attacking mulberry trees in the field. Compared to not-treated or tap-water-spraying control groups, SAC-spraying groups showed significantly higher mortality rates of FWW larvae (56.2%). Thus, SAC can be used for control FWW larvae in mulberry trees.

• Korean Journal of Environmental Biology
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• v.34 no.3
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• pp.169-176
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• 2016

Although chemical fertilizers have a quick effect and broad applicability to agricultural fields, they have caused many problems like increasing soil acidity or decreasing soil organic matters. Environmental-friendly agriculture has been attempted in various ways such as organic agriculture, natural farming, low input and sustainable agriculture. The common interest of all environmental-friendly systems is to decrease burden to agricultural environment by low input of agricultural labor and materials. This study was conducted to estimate overwintering capacity and genetic distance among Chinese Milk Vetch (Astragalus sinicus, CMV) collections based on morphological characteristics and AFLP (Amplified fragment length polymorphism) analysis. Furthermore, the effect of CMV as green manure was observed in mix-cultured paddy fields with rice, sesame and sweet-potato. An another objective of this study was also to compare the pattern of weed occurrence in paddy fields with or without CMV and different rice transplanting times. The CMV collected from Paju district in central region of Korea was successively occurring through self-reseeding without artificial management. However, there was no noticeable difference in growth habit between Paju native CMV and introduced CMV from China which is currently used in farm fields. On the basis of multi-dimensional scaling and tree analyses, there are no significant difference of agricultural growth characteristics among Paju and chinese collections only excepting leaf angle and root length. The flowering time of Gurye collection was fast for 1 week as compared to other collections. AFLP that was commonly used for plant classfication, was applied to exam the genetic variation of CMV collections. Total 579 PCR products and 336 polymorphic fragments were generated using 8 primer pairs.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.479-490
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• 2018

Red pepper is seriously damaged by thrips (Thrips palmi) and anthracnose caused by Colletotrichum acutatum throughout its development. Because of biotic constraints, producers often depend on chemicals that are expensive and have adverse effects on the environment, operator, and beneficial insects. In addition, resistance is developed because of the repeated use of chemicals. In recent decades, the use of microorganisms in crop protection has become a credible alternative because it is eco-friendly. In this study, we aimed to select isolates with insecticidal and fungicidal activities against the pathogens that cause anthracnose and thrips. We treated T. palmi adults and juveniles with 13 strains of entomopathogenic fungi (isolated from the soil by using the insect-bait method), and 6 strains showed excellent insecticidal activity (70-100%) 5 days after the treatment. The selected isolates were cultured with C. acutatum to screen for the strain with excellent anti-fungal activities, among which an isolate FT333 showed more than 95% control efficacy against C. acutatum in vitro. The isolate was identified as Isaria javanica through its morphological characteristics and phylogenetic analysis of the ITS and ${\beta}-tubulin$ nucleotide sequences. The Isaria javanica FT333 isolate could be used effectively for dual bio-control of thrips and anthracnose during red pepper cultivation.