• Mycobiology
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• v.47 no.2
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• pp.200-206
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• 2019

Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier in inbreeding. Molecular markers linked to mating types of P. eryngii KNR2312 were investigated with randomly amplified polymorphic DNA to enhance crossing efficiency. An A4-linked sequence was identified and used to find the adjacent genomic region with the entire motif of the A locus from a contig sequenced by PacBio. The sequence-characterized amplified region marker $7-2_{299}$ distinguished A4 mating-type monokaryons from KNR2312 and other strains. A BLAST search of flanked sequences revealed that the A4 locus had a general feature consisting of the putative HD1 and HD2 genes. Both putative HD transcription factors contain a homeodomain sequence and a nuclear localization sequence; however, valid dimerization motifs were found only in the HD1 protein. The ACAAT motif, which was reported to have relevance to sex determination, was found in the intergenic region. The SCAR marker could be applicable in the classification of mating types in the P. eryngii breeding program, and the A4 locus could be the basis for a multi-allele detection marker.

• The Korean Journal of Food And Nutrition
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• v.33 no.2
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• pp.142-148
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• 2020

The purpose of this study was to investigate the prevalence, toxin gene profiles, and enterotoxin producing ability of Bacillus cereus isolated from environment-friendly vegetables and good agricultural practices (GAP) vegetables. A total of 49 vegetables including 40 environment-friendly vegetables and 9 GAP vegetables were tested. The Vitek 2 system was used to identify B. cereus and the PCR was used to detect 6 toxin genes, respectively. B. cereus was detected in 34 (69.3%) of 49 vegetables and the prevalence of B. cereus in GAP vegetables (44.4%) was lower than in the environment-friendly vegetables (75.0%). The detection rates of entFM, nheA, hblC, and cytK enterotoxin genes, respectively, among all isolates were 100%, 97.0%, 88.2%, and 73.5%, respectively. All of the isolates had at least one or more enterotoxin gene and 20 isolates (58.8%) had hemolysin BL enterotoxin producing ability. The risk of food poisoning from the environment-friendly vegetables and the GAP vegetables has been shown as constant. Thus, it is necessary to expand the supply of GAP vegetables showing lower B. cereus contamination than the environment-friendly vegetables. The characteristics of the environment-friendly vegetables and the GAP vegetables that must be consumed after cleaning should be disseminated to consumers regarding food poisoning prevention.

• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.323-329
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• 2011

BACKGROUND: The conazoles, difenoconazole, diniconazole, hexaconazole, penconazole and tetraconazole are a large class of synthetic fungicides used extensively for foliage and seed treatments in agricultural crops. The extensive use of conazoles has brought concerns on the potentiality of environmental contamination and toxicity. Thus studies on the development of methods for monitoring the conazoles are required. METHODS AND RESULTS: A modified quick, easy, effective, rugged and safe (QuEChERS) method was involved in sample preparation. Quadrapole time of flight mass spectrometer (Q-TOF MS) in electron spray ionization (ESI) mode was employed to determine conazoles in garlic samples. The limit of detection (LOD) and limit of quantification (LOQ) of conazoles by Q-TOF-MS ranged from 0.001 to 0.002 mg/L and 0.002 to 0.005 mg/L, respectively. Q-TOF-MS analysis exhibited less than 2.6 ppm error of accurate mass measurements for the detection of conazoles spiked at 0.05 mg/L in garlic matrix. Recovery values of conazoles fortified in garlic samples at 0.02, 0.05 and 0.1 mg/L were between 79.2 and 106.2% with a maximum 11.8% of standard deviation. No detectable conazoles were found in the domestic market samples by using the Q-TOF-MS method. CONCLUSION(s): High degree of confirmation for conazoles by accurate mass measurements demonstrated that Q-TOF-MS analysis combined with a QuEChERS method may be applicable to simultaneous determination of conazoles in garlic samples.

• Korean Journal of Environmental Agriculture
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• v.42 no.4
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• pp.371-381
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• 2023

The effectiveness of plant-based attractants was studied using large traps, which attracted relatively more snails in agricultural water drainage ditches and rice-cultivating environments, although their effectiveness in rice fields and lakes was limited. The rate began to rise after three hours of observation. Watermelon peel exhibited the highest apple snail attraction rate (13.8%), followed by potatoes (10.0%), and apple peel (8.8%). These values significantly differed from the attraction rate attributed to papaya leaves (F=3.84; P=0.0387). After 24 h, watermelon peel and apple peel indicated a higher rate of attraction (23.4% and 21.7%, respectively), which were significantly different compared with those of papaya leaves and potatoes (F=9.94; P=0.00455). Large bait traps outperformed funnel traps in capturing golden apple snails and trapped a significant number of snails measuring over 1 cm in size. Watermelon peel was the most effective attractant for a large bait trap, followed by apple peel, potatoes, and papaya leaves. On average, 110 snails were captured in the lure net. However, potatoes, apple peels, and papaya leaves caught an average of 93, 80, and 79 snails, respectively. Among the attractants, the lure effect of the snails was not significantly different. The efficiency of large bait traps in capturing snails, regardless of the plant attractant employed, followed the order: apple peel > watermelon peel and potatoes > papaya leaves > melon > Korean melon. Watermelon peel is highly recommended for farmer use, as well as apple peel and potatoes. Utilizing these snail attractants may contribute positively to developing a safe and environment-friendly integrated pest management strategy.

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.09a
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• pp.3-33
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• 2001

A review on the technical development and agricultural policy direction for sustainable agriculture has been made to clarify the present conditions of agricultural environment for food production and natural resource conservation and to study prospects and solutions successfully to achieve food safety and environment conservation as the 21st agricultural paradigm. Although great improvement of environmentally sound and resource-conserving techniques, sustainability of Korean agriculture has been damaged by quantitative reduction and quality deterioration of agricultural land resources, insufficient implementation of low-input and organic matter recycling techniques and also improper cropping system. It is concluded that goal of sustainable agriculture can be successfully achieved by further improvement of environmentally friendly techniques, and stronger escalation of national implementation programmes with more financial supports including direct payments for sustainability of agriculture under systematical support and cooperation of AKS (agricultural knowledge system).

• The Plant Pathology Journal
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• v.22 no.2
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• pp.147-151
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• 2006

Disease incidences of cucumber virus diseases in Jeonnam province were 52.5%, 16.1%, 35.2%, and 50.9% in 1999, 2000, 2001, and 2002, respectively. Rod- and flexuous rod-shaped virus particles were observed with the frequencies of 63.2% and 10.5%, respectively from the samples collected in 1999 under EM observation. Rod-shaped virus particles are considered as tobamovirus while flexuous rod shaped particles are considered as potyviruses. To further confirm their nature, total of 312 diseased virus samples were collected from 2000 to 2002, and tested by RT-PCR. Disease incidences of tobamoviruses including Cucumber green mottle mosaic virus and Kyuri green mottle mosaic virus were 48.7% and 3.8%, respectively while those of potyviruses including Zucchini yellow mosaic virus, Papaya ringspot virus, and Watermelon mosaic virus were 15.7%, 9.3%, and 5.1%, respectively. Interestingly, Cucumber mosaic virus was hardly detected. About 5.8% of tested samples were infected with more than one virus. Tobamovirus infection was consistently observed from September to December regardless of planting time, whereas infection of potyviruses was observed in many cucumber cultivating areas where it was planted in September and October.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1999.06a
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• pp.53-80
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• 1999

Productivity and environmental conservation in nowadays trade-off and serious problem in agriculture. Precision farming is developing for solving the trade-off problem using systems approach and variable management. The systems approach is attributed to aiming at information-oriented agriculture, environmental-friendly sustainable agriculture, and complex system optimization . The variable management is composed of describing variability , variable-rate technology and decision support system. Three levels of technology development and three farming strategies are introduced for having a prospect. Describing the variability is the first step to promote it. Precision farming could be available for small scale farming as well as big scale farming. Paddy field precision farming will undergo in its distinctive way.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.453-457
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• 2016

This study investigates the hatching periods and hatchability of the eggs of Pochazia shantungensis at different collection times from 2011 to 2014, and the effect of temperature on the growth of P. shantungensis nymphs in an area of its outbreak. The hatchability of P. shantungensis eggs varies with their collection time; their hatchability in late November was higher than that in March of the next year, but no difference was observed in their hatching periods. The hatching periods of the eggs were 51.2, 31.3, 24.8, 19.4, 17.1, and 19.4 days at 15, 18, 21, 24, 27, and $30^{\circ}C$, respectively. The hatchability was above 70% at temperatures ranging from 18 to $27^{\circ}C$. The hatching time of the overwintered eggs in the Gurye region in Korea was reduced by 9 days from 2011 to 2014. The hatching rate was relatively higher when the average temperature in the winter season was relatively warmer. The dvelopmental periods of the first to fifth nymphs were 82.8, 58.0, 45.8, and 39.6 days at 18, 21, 24, and $27^{\circ}C$, respectively, at the relative humidity of 40~70%, and a photoperiod off 14 h light:10 h dark. The higher the temperature, the shorter the developmental period. At $30^{\circ}C$, all life stages after the fourth nymph died. Thus, the optimum growth temperature was estimated to be $27^{\circ}C$. For all life stages from the egg to the fourth nymph, the relationship between the temperature and developmental rate was expressed by the linear equation Y = 0.0015 X - 0.014. The lower developmental threshold was $9.3^{\circ}C$ and the effective cumulative temperature was 693.3 degree-days. The lower developmental threshold of approximately $3.8^{\circ}C$ was the lowest at the fourth nymph stage.

• Korean journal of applied entomology
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• v.54 no.3
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• pp.191-197
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• 2015

Fruit drop due to Eurytoma maslovskii infestations of Japanese apricot (Prunus mume) is a serious economic issue in most parts of Jeonnam Province, with the exception of the coastal areas such as Wando, Shinan, Yeosu, and Muan. The average incidence of fruit drop was 67% in 2013 and 33.3% in 2014. E. maslovskii larvae overwinter inside the pits of Japanese apricots. Larval survival rates decrease to less than 30% after a rainy summer season. The eggs are long oval of 0.68 mm, 0.29 mm and ivory white. Mature larva is of 6.56 mm, 3.18 mm. Free pupa are black, adult females and males of 6.97 mm, 4.90 mm lengths, respectively, while the ovipositor is 0.64 mm in length. Adult emergence occurs from early April to early May, when Japanese apricot fruits are from 4 mm to 17 mm in diameter. Adults persist for 13.5 d after emergence, and the sex ratio of females to males was found th be 45.9:54.1. Although up to four eggs may be oviposited per fruit, only one larva will ultimately survive, ans the larvae are cannibalistic. The period during which E. maslovskii is able to oviposit on Japanese apricots only lasts from mid- to late April (fruit diameter: 12~16 mm). When the diameter of the fruit is >16 mm, the pit of the Japanese apricot hardens and larvae have difficultly penetrating the stone. Therefore, the most effective method of controlling this pest is to spray orchards with a control agent 2~3 times, at 5 d intervals, beginning in mid- April.

• Korean journal of applied entomology
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• v.57 no.4
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• pp.347-354
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• 2018

In order to setting the control standard of Chinese cabbage pests using a drone, the downward wind speed, spraying width, and the number of falling particles and particle size were examined using a water sensitive paper with spray different heights (3, 4, 5 m) and flying speeds (3, 4 m/sec). Fore kinds of pesticides for aviation control were used to test the perfect lethal concentration and dose for major pests of Chinese cabbage such as Plutella xylostella, Spodoptera exigua and Spodoptera litura. The number of falling particles in spraying pesticides with drones was 80.5% on the upper side, 14.8% on the vertical side, and 4.7% on the back side. The number of falling particles as different spray heights were 3 m = 53, 4 m = 40 and $5m=39particles\;cm^{-2}$. The number of falling particles as different flying speeds were $3m\;sec^{-1}=62$ and $4m\;sec^{-1}=25particles\;cm^{-2}$. In the laboratory test, the perfect lethal concentration and dose of Plutella xylostella was chlorfenapyr SC (20 times, $0.5{\mu}l$) and bistrifluron chlorfenapyr SC (25 times, $0.5{\mu}l$). The perfect lethal concentration and dose of Spodoptera exigua was chlorfenapyr SC (20 times, $1{\mu}l$), bistrifluron chlorfenapyr SC (20 times, $1{\mu}l$), and chlorfenapyr SC (20 times, $1{\mu}l$) and bistrifluron chlorfenapyr SC (20 times, $0.5{\mu}l$) for Spodoptera litura. Therefore, the main pest control method of Chinese cabbage using drones is 20 times diluted chlorphenapyr SC or bistrifluoruron-chlorphenapyr SC, sprayed at 3 m height by $3msec^{-1}$ of going speed. This spraying method will be effective for control of Chinese cabbage pest.