The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Prevalence and Toxin Characteristics of Bacillus cereus Isolated from Vegetables in Gwangju Metropolitan City

광주지역 유통 채소류의 Bacillus cereus 오염실태 및 독소 특성

  • Cho, Sun-Ju (Food Microbiology Division, Health and Environment Research Institute of Gwangju) ;
  • Jeong, So-Hyang (Food Microbiology Division, Health and Environment Research Institute of Gwangju) ;
  • Seo, Yu-Jin (Food Microbiology Division, Health and Environment Research Institute of Gwangju) ;
  • Kim, Tae-Sun (Food Microbiology Division, Health and Environment Research Institute of Gwangju) ;
  • Lee, Hyang-Hee (Food Microbiology Division, Health and Environment Research Institute of Gwangju) ;
  • Lee, Min-Gyou (Food Microbiology Division, Health and Environment Research Institute of Gwangju) ;
  • Seo, Jung-Mi (Food Microbiology Division, Health and Environment Research Institute of Gwangju) ;
  • Cho, Bae-Sik (Food and Drugs Research Department, Health and Environment Research Institute of Gwangju) ;
  • Kim, Joung-Beom (Department of Food Science and Technology, Sunchon National University)
  • 조선주 (광주광역시보건환경연구원 식품미생물과) ;
  • 정소향 (광주광역시보건환경연구원 식품미생물과) ;
  • 서유진 (광주광역시보건환경연구원 식품미생물과) ;
  • 김태순 (광주광역시보건환경연구원 식품미생물과) ;
  • 이향희 (광주광역시보건환경연구원 식품미생물과) ;
  • 이민규 (광주광역시보건환경연구원 식품미생물과) ;
  • 서정미 (광주광역시보건환경연구원 식품미생물과) ;
  • 조배식 (광주광역시보건환경연구원 식품의약품연구부) ;
  • 김중범 (순천대학교 식품공학과)
  • Received : 2020.03.04
  • Accepted : 2020.03.26
  • Published : 2020.04.30
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Abstract

The purpose of this study was to investigate the prevalence, toxin gene profiles, and enterotoxin producing ability of Bacillus cereus isolated from environment-friendly vegetables and good agricultural practices (GAP) vegetables. A total of 49 vegetables including 40 environment-friendly vegetables and 9 GAP vegetables were tested. The Vitek 2 system was used to identify B. cereus and the PCR was used to detect 6 toxin genes, respectively. B. cereus was detected in 34 (69.3%) of 49 vegetables and the prevalence of B. cereus in GAP vegetables (44.4%) was lower than in the environment-friendly vegetables (75.0%). The detection rates of entFM, nheA, hblC, and cytK enterotoxin genes, respectively, among all isolates were 100%, 97.0%, 88.2%, and 73.5%, respectively. All of the isolates had at least one or more enterotoxin gene and 20 isolates (58.8%) had hemolysin BL enterotoxin producing ability. The risk of food poisoning from the environment-friendly vegetables and the GAP vegetables has been shown as constant. Thus, it is necessary to expand the supply of GAP vegetables showing lower B. cereus contamination than the environment-friendly vegetables. The characteristics of the environment-friendly vegetables and the GAP vegetables that must be consumed after cleaning should be disseminated to consumers regarding food poisoning prevention.

Keywords

References

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