• Applied Microscopy
• /
• v.29 no.3
• /
• pp.343-351
• /
• 1999

The structures of the fertilized egg envelope from three species, three-spot gourami (Trichogaster trichopterus), pearl gourami (Trichogaster leeri) and marble gourami (Trichogaster trichopterus trichopterus) belong to Belontiidae were observed, utilizing light, scanning and transmission electron microscopes. In all three species, the fertilized eggs were the colorless, transparent, spherical, adhesive and pelagic type. A large oil droplet was located in vitelline membrane of the fertilized egg. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. Specially, the micropyle of three-spot gourami was similar to that of marble gourami which is subspecies of three-spot gourami. An outer surface of the fertilized egg envelope was arranged by grooves in all three species. The fertilized egg envelopes consists of two distinct layers; an adhesive outer layer and an inner layer with high electron density. In conclusion, the morphological similarity of the fertilized egg, micropyle, outer surface and transverse section of the fertilized e9g envelope seems to be an indication of the Belontiidae.

• Korean Journal of Computational Design and Engineering
• /
• v.2 no.4
• /
• pp.253-266
• /
• 1997

Pencil-curve machining, which is a single-pass ball-end milling along a concave edge on adie surface, is widely employed in die-surface machining. The cutter-path used for pencil-curve machining, which is the trajectory of the “ball-center point” of a ball-endmill sliding along a concave-edge region on the die surface, is called pencil-curve. Presented in the paper is a pencil-curve tracing algorithm in which “concave-type” sharp edges are computed from a “virtually digitized” model of the tool-envelope surface. The resulting “initial” pencil-cures are then refuted by applying a series of fairing operations. illustrative examples and methods for enhancing accuracy are also presented. The proposed pencil-curve tracing algorithm has been successfully implemented in a commercial CAM system specialized in die-machining and in the CAD/CAM system CATIA.

• Journal of Institute of Convergence Technology
• /
• v.3 no.1
• /
• pp.1-8
• /
• 2013

High-precision surface actuator, in which in-plane motion is realized by not two-dimensional actuator superposing linear actuators but integrated planar actuator, has been developed to cope with the severe target performance like precise motion with large envelope. It is very difficult to accomplish the performance with the traditional actuating principle. So, various methods have been tried to break through the problem. This paper discusses some meaningful trials performed in the Nano Measurement and Precision Motion Control Lab. of Korea National University of Transportation.

• Journal of Korean Institute of Industrial Engineers
• /
• v.19 no.3
• /
• pp.123-137
• /
• 1993

In this paper, an integrated approach is proposed to generate gouging-free Cartesian tool paths for machining sculptured surfaces from 3D measurement data. The integrated CAD/CAM system consists of two modules : offset surface module an Carteian tool path module. The offset surface module generates an offset surface of an object from its 3D measurement data, using an offsetting method and a surface fitting method. The offsetting is based on the idea that the envelope of an inversed tool generates an offset surface without self-intersection as the center of the inversed tool moves along on the surface of an object. The surface-fitting is the process of constructing a compact representation to model the surface of an object based on a fairly large number of data points. The resulting offset surtace is a composite Bezier surface without self-intersection. When an appropriate tool-approach direction is selected, the tool path module generates the Cartesian tool paths while the deviation of the tool paths from the surface stays within the user-specified tolerance. The tool path module is a two-step process. The first step adaptively subdivides the offset surface into subpatches until the thickness of each subpatch is small enough to satisfy the user-defined tolerance. The second step generates the Cartesian tool paths by calculating the intersection of the slicing planes and the adaptively subdivided subpatches. This tool path generation approach generates the gouging-free Cartesian CL tool paths, and optimizes the cutter movements by minimizing the number of interpolated points.

• Journal of the Korean Solar Energy Society
• /
• v.23 no.1
• /
• pp.1-8
• /
• 2003

Various efforts to combine new high-tech materials with solar system have been progressed nowadays in order to improve the performance of the existing passive solar system. TIM(Transparent Insulation Material) replacing the conventional outer building envelope glazing as well as the wall is good example for this trend. TI integrated wall is a thermal mass wall with a special shaped TIM instead of using typical envelope materials The tested TIM type is a small(diameter 4mm and thickness 50mm) capillary tube of Okalux model and cement brick(density 1500kg/m3). The purpose of this study was to analyze the thermal performance through the actual measurements performed in a test cell. This study was carried out to justify the following issues. 1) the impact of Tl-wall over the temperature variations 2) the impact of mass wall surface absorptance over the transient thermal behavior and 3) the impact of thermal mass wall thickness over the temperature variations. Finally, as results indicated that the peak time of room temperature was shifted about one hour early when absorptance of thermal mass wall changed from 60% to 95% for the 190mm thickness thermal mass wall test case. the temperature difference of both surfaces of thermal mass wall surface showed about $23^{\circ}C$ during a day of March for the 380mm thickness thermal mass wall case. However, the thermal mass wall was over-heated by outside temperature and solar radiation in a day of May the temperature difference of both surfaces of thermal mass wall surface was indicated $10^{\circ}C$ and inside temperature was observed more than average 22C.

• Journal of the Korea Institute of Building Construction
• /
• v.16 no.6
• /
• pp.579-585
• /
• 2016

3D laser scanning can be used for scanning the freeform surface and building a model from which the measurements could be taken, in order to solve the difficulty with getting access to the exact freeform shape and position data of the complex building envelope. The shape making process using 3D scanning is as follows: point cloud, mesh surface segmentation, NURBS(Non-Uniform Rational B-spline) surface generation, and parametric solid model generation. In this research, we review previous studies, reverse engineering notion, importance of reverse engineering usage for freeform envelope, and previous cases in order to identify the detail reverse engineering process for prefabrication and construction of freeform panels using 3D laser scanning technology. Therefore, the purpose of this research is to present a basic information which should be considered during design and construction phase and improve quality and constructibility of freeform building by analyzing the reverse engineering process and case study for prefabrication and construction of freeform panels using 3D laser scanning. The research results will enable 3D shape engineering and design parameterization using reverse engineering to be used in various construction projects.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.11 no.2
• /
• pp.173-177
• /
• 2006

In this study, a novel strategy was developed for the highly selective immobilization of proteins, using the polyhydroxyalkanoate (PHA) depolymerase substrate binding domain (SBD) as an active binding domain. In order to determine the appropriacy of this method for immunodiagnostic assays, the single-chain antibody (ScFv) against the hepatitis B virus (HBV) preS2 surface protein and the severe acute respiratory syndrome coronavirus (SARS-CoV) envelope protein (SCVe) were fused to the SBD, then directly immobilized on PH A-coated slides via microspotting. The fluorescence-labeled HBV antigen and the antibody against SCVe were then utilized to examine specific interactions on the PHA-coated surfaces. Fluorescence signals were detected only at the spotted positions, thereby indicating a high degree of affinity and selectivity for their corresponding antigens/antibodies. Furthermore, we detected small amounts of ScFv-SBD (2.7 ng/mL) and SCVe-SBD fusion proteins (0.6ng/mL). Therefore, this microarray platform technology, using PHA and SBD, appears generally appropriate for immunodiagnosis, with no special requirements with regard to synthetic or chemical modification of the biomolecules or the solid surface.

• Korean Journal of Materials Research
• /
• v.10 no.10
• /
• pp.698-702
• /
• 2000

The characteristics of optical absorption in $Al_{0.24}Ga_{0.76}As/GaAs$ multi-quantum wells(MQWs) structure were investigated by using the surface photovoltage(SPV). The Spy features near 1.42 eV showed two overlapping signals. By chemical etching, we found associated with the GaAs substrate and the GaAs cap layer. The Al composition(x=24 %) was determined by Kuech's composition formula. In order to identify the transition energies. the experimentally observed energies were compared with results of the envelope function approximation for a rectangular quantum wells An amplitude variation of the relative Spy intensity from the GaAs substrate, llH, and llL was observed at different light intensities. A variation in the SPY line shape of the transition energies were observed with decreasing tempera­t ture.

• Korean Journal of Ichthyology
• /
• v.26 no.3
• /
• pp.179-184
• /
• 2014

A histological study on the egg envelope and oogenesis of Gobiobotia brevibarba (Pisces, Cyprinidae) was carried out by a light microscope and a scanning electron microscope. Various developmental cells appeared in the ovary caught during May 2014, spawning season. For the relative area of oocyte, the ovary consisted of mature stage (74.5%), a vitellogenic stage (yolk granule stage, 16.6% and yolk vesicle stage, 6.6%) and previtellogenic stage (perinucleolus stage 2.2%), which means its spawning season. The cytoplasm of the perinucleolus oocyte is acidic and many nucleoli are located at the inner side of the nuclear membrane. The yolk vesicles, an early vitellogenic stage, has a follicular layer and a zona radiata clearly. Numerous villi, called an egg envelope, begin to form on the zona radiata. The yolk granules, an another vitellogenic stage, proceeds and they show a strong eosinophilic nature. Such yolk granules appeared between the yolk vesicles occupying most cytoplasm, and as the stage proceeds, there are some yolk masses fused with each other. Egg envelope is covered with plenty of villi ($2{\sim}3{\mu}m$ in the length) over the entire egg surface.

• Applied Microscopy
• /
• v.39 no.1
• /
• pp.9-15
• /
• 2009

The ultrastructure of the fertilized egg envelope in Cichlasoma managuensis belonging to Cichlidae were investigated by routine light and electron microscopes. The fertilized eggs of Cichlasoma managuensis was of the light yellow, non-transparent, ellipsoidal, adhesive and nonfloted type. The size of fertilized egg was the major axis $1.92{\pm}0.08\;mm$, the minor axis $1.43{\pm}0.04\;mm$. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. An outer surface of fertilized egg envelope was covered by a adhesive reticular structures and the fertilized egg envelopes consisted of two distinct layers; an outer adhesive layer and an inner layer of $13{\sim}15$ horizontal lower electron density lamellae alternating with interlamellae of higher electron density. The external shape of fertilized egg is common trait of fishes belonging to Cichlidae and these ultrastructural characters of fertilized egg envelope can be utilized in taxonomy of teleost.