• Animal Bioscience
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• v.37 no.3
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• pp.437-450
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• 2024

Objective: Vanin-1 (VNN1) is a pantetheinase that catalyses the hydrolysis of pantetheine to produce pantothenic acid and cysteamine. Our previous studies have shown that the VNN1 is specifically expressed in chicken liver which negatively regulated by microRNA-122. However, the functions of the VNN1 in lipid metabolism in chicken liver haven't been elucidated. Methods: First, we detected the VNN1 mRNA expression in 4-week chickens which were fasted 24 hours. Next, knocked out VNN1 via CRISPR/Cas9 system in the chicken Leghorn Male Hepatoma cell line. Detected the lipid deposition via oil red staining and analysis the content of triglycerides (TG), low-density lipoprotein-C (LDL-C), and high-density lipoprotein-C (HDL-C) after VNN1 knockout in Leghorn Male Hepatoma cell line. Then we captured various differentially expressed genes (DEGs) between VNN1-modified LMH cells and original LMH cells by RNA-seq. Results: Firstly, fasting-induced expression of VNN1. Meanwhile, we successfully used the CRISPR/Cas9 system to achieve targeted mutations of the VNN1 in the chicken LMH cell line. Moreover, the expression level of VNN1 mRNA in LMH-KO-VNN1 cells decreased compared with that in the wild-type LMH cells (p<0.0001). Compared with control, lipid deposition was decreased after knockout VNN1 via oil red staining, meanwhile, the contents of TG and LDL-C were significantly reduced, and the content of HDL-C was increased in LMH-KO-VNN1 cells. Transcriptome sequencing showed that there were 1,335 DEGs between LMH-KO-VNN1 cells and original LMH cells. Of these DEGs, 431 were upregulated, and 904 were downregulated. Gene ontology analyses of all DEGs showed that the lipid metabolism-related pathways, such as fatty acid biosynthesis and long-chain fatty acid biosynthesis, were enriched. KEGG pathway analyses showed that "lipid metabolism pathway", "energy metabolism", and "carbohydrate metabolism" were enriched. A total of 76 DEGs were involved in these pathways, of which 29 genes were upregulated (such as cytochrome P450 family 7 subfamily A member 1, ELOVL fatty acid elongase 2, and apolipoprotein A4) and 47 genes were downregulated (such as phosphoenolpyruvate carboxykinase 1) by VNN1 knockout in the LMH cells. Conclusion: These results suggest that VNN1 plays an important role in coordinating lipid metabolism in the chicken liver.

• Journal of Korean Elementary Science Education
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• v.43 no.1
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• pp.108-121
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• 2024

In this study, we developed a sustainability education program employing a project-based learning strategy for prospective teachers and investigated its effectiveness. A total of 23 senior students from a university of education participated in the study. The investigation involved a pretest on their pro-environmental behavior and attitudes, followed by a five-week implementation of the program, during which students individually engaged in energy-saving projects. Following the program, a post-test, which used the same questionnaire as the pretest, was administered. In addition, we conducted individual interviews with nine students who actively engaged in the projects. We analyzed the interview contents, portfolios, and reports; identified sub-concepts related to the program's effectiveness and its causes; and then organized them into subcategories. Then, we extracted recurring relationships among the subcategories to formulate a tentative explanatory model. The results indicate that the program positively impacted students' pro-environmental behavior and values/attitudes. Notably, the students' "sense of achievement gained through success" emerged as a significant factor influencing their pro-environmental behavior. Furthermore, some causes were found to indirectly affect pro-environmental behavior through pro-environmental values and attitudes.

• Advances in nano research
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• v.16 no.4
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• pp.325-340
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• 2024

There are several novel uses for dispersing many nanoparticles into a conventional fluid, including dynamic sealing, damping, heat dissipation, microfluidics, and more. Therefore, melting heat and mass transfer characteristics of a 3-D MHD Hybrid Nanofluid flow over a rotating disc with presenting dufour and soret effects are assessed numerically in this study. In this instance, we investigated both ferric sulfate and molybdenum disulfide as nanoparticles suspended within base fluid water. The governing partial differential equations are transformed into linked higher-order non-linear ordinary differential equations by the local similarity transformation. The collection of these deduced equations is then resolved using a Chebyshev spectral collocation-based algorithm built into the Mathematica software. To demonstrate how different instances of hybrid/ nanofluid are impacted by changes in temperature, velocity, and the distribution of nanoparticle concentration, examples of graphical and numerical data are given. For many values of the material parameters, the computational findings are shown. Simulations conducted for different physical parameters in the model show that adding hybrid nanoparticle to the fluid mixture increases heat transfer in comparison to simple nanofluids. It has been identified that hybrid nanoparticles, as opposed to single-type nanoparticles, need to be taken into consideration to create an effective thermal system. Furthermore, porosity lowers the velocities of simple and hybrid nanofluids in both cases. Additionally, results show that the drag force from skin friction causes the nanoparticle fluid to travel more slowly than the hybrid nanoparticle fluid. The findings also demonstrate that suction factors like magnetic and porosity parameters, as well as nanoparticles, raise the skin friction coefficient. Furthermore, It indicates that the outcomes from different flow scenarios correlate and are in strong agreement with the findings from the published literature. Bar chart depictions are altered by changes in flow rates. Moreover, the results confirm doctors' views to prescribe hybrid nanoparticle and particle nanoparticle contents for achalasia patients and also those who suffer from esophageal stricture and tumors. The results of this study can also be applied to the energy generated by the melting disc surface, which has a variety of industrial uses. These include, but are not limited to, the preparation of semiconductor materials, the solidification of magma, the melting of permafrost, and the refreezing of frozen land.

• Animal Bioscience
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• v.37 no.8
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• pp.1387-1397
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• 2024

Objective: The objective of the current study was to find out the independent and interactive effects of prilled fat supplementation with protein on the production performance of early lactating Nili Ravi buffaloes. Methods: Sixteen early lactating buffaloes (36.75±5.79 d in milk; mean±standard error) received 4 treatments in 4×4 Latin-square design according to 2×2 factorial arrangements. The dietary treatments were: i) low protein low fat, ii) low protein high fat, iii) high protein low fat, and iv) high protein high fat. The dietary treatments contained 2 protein (8.7% and 11.7% crude protein) and fat levels (2.6% and 4.6% ether extract) on a dry matter basis. Results: The yields of milk and fat increased with increasing protein and fat independently (p≤0.05). Energy-, protein-, and fat-corrected milk yields also increased with increasing protein and fat independently (p≤0.05). Increasing dietary protein increased the protein yield by 3.75% and lactose yield by 3.15% and increasing dietary fat supplies increased the fat contents by 3.93% (p≤0.05). Milk yield and fat-corrected milk to dry matter intake ratios were increased at high protein and high fat levels (p≤0.05). Milk nitrogen efficiency was unaffected by dietary fat (p>0.10), whereas it decreased with increasing protein supplies (p≤0.05). Plasma urea nitrogen and cholesterol were increased by increasing protein and fat levels, respectively (p≤0.05). The values of predicted methane production reduced with increasing dietary protein and fat. Conclusion: It is concluded that prilled fat and protein supplies increased milk and fat yield along with increased ratios of milk yield and fat-corrected milk yields to dry matter intake. However, no interaction was observed between prilled fat and protein supplementation for production parameters, body weight, body condition score and blood metabolites. Predicted methane production decreased with increasing protein and fat levels.

• Journal of the Korean Magnetics Society
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• v.12 no.5
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• pp.189-194
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• 2002

Microstructure and magnetic properties of Sm-Co sintered magnet were investigated with the variation of Zr content and their solution treatment and aging temperatures. The fraction of eutectic structure and the size of eutectic area decreased with increasing x value of cast Sm(C $O_{.688-x}$F $e_{.242}$C $u_{.07}$Z $r_{x}$)$_{7.404}$ alloys. On the other hand, x=0.022 ingot had finer dendritic structure compared to the other alloys. The sintered magnet of Sm(C $O_{.688-x}$F $e_{.242}$C $u_{.07}$Z $r_{x}$)$_{7.404}$ had well defined cell structure which is composed of cell boundary Sm $Co_{5}$ and cell interior S $m_2$Co/ssub 17/ phase. Cell boundary Sm $Co_{5}$ phase has 20nm thickness and its relative angle was 120$^{\circ}$ in x=0.018 and 0.022 alloys. Cell size was decreased with increasing Zr contents. But, x=0.026 alloy has diffuse cell boundary and irregular shape compared to x=0.022 and 0.018 alloys. Maximum value of coercive force and maximum energy Product were obtained from x=0.022 alloys. Optimum solution treatment temperature of Sm(C $O_{.688-x}$F $e_{.242}$C $u_{.07}$Z $r_{x}$)$_{7.404}$ alloy was 1170 $^{\circ}C$ and 1st aging temperature of two step aging process for higher coercivity was 850 $^{\circ}C$.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.6
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• pp.751-758
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• 2017

The objective of this study was to determine the effects of moisture content and screw speed on the physical properties of extruded soy protein isolate (SPI). Expansion index, water absorption index, texture, integrity index, color, and nitrogen solubility index of extruded SPI were analyzed to determine the relationship with extrusion conditions. Extrusion conditions were moisture content (40, 50, and 60%) at a fixed die temperature ($140^{\circ}C$) and screw speed (250 rpm). The other extrusion conditions were screw speed (150, 250, and 330 rpm) at a fixed moisture content (55%) and die temperature ($140^{\circ}C$). Specific mechanical energy (SME) input decreased as moisture content increased from 40 to 60%. However, SME input increased as screw speed increased from 150 to 330 rpm. Expansion ratio and piece density decreased as moisture content and screw speed increased, and specific length increased as moisture content and screw speed increased. The extruded SPI at 40% moisture content had higher water absorption index, texture, and color differences than those of the extruded SPI at other moisture contents (50 and 60%). however, the extruded SPI at 40% moisture content had lower integrity index and cutting strength than those of the extruded SPI at other moisture contents (50 and 60%). In conclusion, the physical properties of extruded SPI were more affected by moisture content than screw speed.

• Economic and Environmental Geology
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• v.40 no.6
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• pp.727-738
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• 2007

The present study investigation the geochemical characteristics of the stream sediments in the Unbong area was conducted to enable a understanding the natural background and a prediction the prospects of geochemical disaster as a result of that bed rocks(mylonitic granites, Kim et al., 1992). We systematically collected seventy three stream sediments samples by wet sieving along the primary channels. Major, trace and rare earth element(REE) concentrations, combined with mineralogical characteristics, were determined by XRD, XRF, ICP-AES and NAA analysis methods. Major element concentrations for the stream sediments in the Unbong area were $SiO_2\;36.94{\sim}65.39wt.%,\;Al_2O_3\;10.15{\sim}21.77wt.%,\;Fe_2O_3\;3.17{\sim}10.90wt.%,\;CaO\;0.55{\sim}5.27wt.%,\;MgO\;0.52{\sim}4.94wt.%,\;K_2O\;1.38{\sim}4.54wt.%,\;Na_2O\;0.49{\sim}3.36wt.%,\;TiO_2\;0.39{\sim}1.27wt.%,\;MnO\;0.04{\sim}0.22wt.%,\;P_2O_5\;0.08{\sim}0.54wt.%$. Trace and REE concentrations for the stream sediments were $Cu\;4.8{\sim}134ppm,\;Pb\;24.2{\sim}82.5ppm,\;Sr\;95.9{\sim}739ppm,\;V\;19.9{\sim}124ppm,\;Zr\;52.9{\sim}145ppm,\;Li\;25.2{\sim}3.3ppm,\;Co\;3.87{\sim}50.0ppm,\;Cr\;17.4{\sim}234ppm,\;Hf\;3.93{\sim}25.2ppm,\;Sc\;4.60{\sim}20.6ppm,\;Th\;3.82{\sim}36.9ppm,\;Ce\;45.7{\sim}243ppm,\;Eu\;0.89{\sim}2.69ppm,\;Yb\;1.42{\sim}5.18ppm$. According to the comparison of average major element concentrations, CaO, $Na_2O\;and\;K_2O$ contents are higher in stream sediments than in bed rocks(mylonitic granites, Kim et al., 1992) $Al_2O_3\;and\;SiO_2$ contents show good correlation both stream sediments and bed rocks(mylonitic granites, Kim et al., 1992). Yb and Eu in the stream sediments show a positive correlation with $SiO_2$. In contrast, the stream sediments display a negative correlation.

• Journal of the Korean Wood Science and Technology
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• v.43 no.3
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• pp.374-380
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• 2015

The quality characteristics of the woodpellet manufactured from two domestic pines (Pinus densiflora S. et Z. and Pinus rigida Mill.) were investigated for the efficient energy use of woody biomass resources. Properties of woodpellets such as moisture content, heating value, ash content, apparent density and durability were determined by using the standard test method of woodpellets of Korea Forest Research Institute (KFRI) and elemental analysis. The results of elemental analysis for C, H, O and N showed 61.42% carbon, 5.56% hydrogen, 32.87% oxygen, and 0.15% nitrogen for Pinus densiflora S. et Z. and 61.03% carbon, 5.96% hydrogen, 32.83% oxygen, and 0.18% nitrogen for Pinus rigida Mill. No significant difference between Pinus densiflora S. et Z. and Pinus rigida Mill was observed on elemental analysis. Heating values of each woodpellet were ranged from 19.00 to 19.42 MJ/kg which satisfied the first grade quality standard (${\geq}18.0MJ/kg$) by KFRI. The ash contents of woodpellet were slightly different between Pinus densiflora S. et Z. and Pinus rigida Mill., and satisfied the first grade quality standard (${\leq}0.7%$) by KFRI. Apparent density of woodpellet (Pinus densiflora S. et Z.) was passed the first grade standard level (${\geq}640kg/m^3$), and woodpellets from Pinus rigida Mill. satisfied the second grade quality of the standard. The moisture contents of each woodpellet were satisfied by the first grade quality standard (${\leq}10%$). The durability of woodpellet (Pinus densiflora S. et Z.) was passed the third grade level (${\geq}95%$), but Pinus rigida Mill. woodpellet was insufficient to satisfy the quality standard.

• Journal of the Mineralogical Society of Korea
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• v.30 no.3
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• pp.93-102
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• 2017

Heavy mineral provide an important information for sediment provenance as well as a potential submarine mineral resources. We compared the heavy mineral characteristics between Southeastern Yellow Sea Mud (SEYSM) and Southwestern Cheju Island Mud (SWCIM) surface sediments. We separated heavy minerals from 28 surface sediments in each mudbelt, and then carried out stereo-microscopic, field-emission scanning electron microscopic, energy dispersive spectroscopic and electron probe microanalysis to characterize the type, abundance, mineralogical properties and distribution pattern of heavy mineral. Amphibole and epidote, which are two major heavy minerals, account for more than 70% of total heavy minerals. Zircon and sphene contents are more abundant in SEYSM, whereas apatite and rutile contents are more abundant in SWCIM. Monazite only occurs in some area of SEYSM. Sphene and monazite content decrease to the south in SEYSM. Both garnet-zircon index (GZi) and rutile-zircon index (RuZi) are low in SEYSM but high in SWCIM. Amphiboles in SEYSM primarily correspond to hornblende, however those in SWCIM represent variable composition from pargasite, tshermakite, hornblende to tremolite. Garnets in SEYSM have high Mg and low Ca, but those in SWCIM have low Mg with variable Ca. Different heavy mineral characteristics between SEYSM and SWCIM suggests that sediments in each mudbelt have different provenances. Although this study implies that SEYSM sediment may mostly come from nearby Korean western rivers such as the Keum and Han rivers, this study does not suggest any idea of the source area of SWCIM sediment. Further study is needed to interpret the provenance and transportation mechanism of mudbelt sediments through the heavy mineral research for the river sediments flowing into the Yellow Sea and much more marine sediments.

• Korean Journal of Organic Agriculture
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• v.19 no.2
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• pp.199-214
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• 2011

This study was conducted to investigate the effect of organic dietary metabolizable energy (ME) and crude protein (CP) on productivity, meat quality and blood composition of crossbred chicks (hanhyub 3 ho) for different growring periods. Experiments were factorially designed with ME 3,000, 3,100kcal/kg and CP 21, 22, 23% for starter (0-4wks); ME 3,100, 3,150, 3,200kcal/kg and CP 18, 19, 20% for grower (5-8wks); ME 3,100, 3,150, 3,200kcal/kg and CP 15, 16, 17% for finisher (9-10wks). The total number of chicks and replicate of each treatment for starter, grower, finisher were 720, 4; 702, 6; 468, 4, respectively. The diets was mixed with more 90% organic feed ingredients. The productivity were not influence by dietary ME for starting period. Weight gain and feed intake were significantly increased in CP 23% treatment than CP 21, 22% treatment (P<0.05). FCR was improved as dietary CP increased (P<0.05). Weight gain was tended to be increased by decreaing ME content and increasing CP contents for growing period. Feed intake showed no difference among the ME and CP treatment groups. FCR improved significantly in CP 19, 20% compared with CP 18% (P<0.05). Weight gain was not significantly different between ME and CP treatments for finishing period. Feed intake was lower in ME 3,150, 3,200kcal/kg than the ME 3,100 kcal/kg treatment (P<0.05). FCR was higher in ME 3,100kcal/kg than ME 3,150, 3,200kcal/kg treatment (P<0.05). Interaction on productivity was not exited between ME and CP treatment groups for different feeding periods. There was no difference in the total protein, albumin, glucose and triglyceride amount in blood depending on dietary ME and CP contents. Total cholesterol was greatly decreased in ME 3,100kcal/kg than the ME 3,200kcal/kg (P<0.05), but not different between CP treatments. Cooking loss of breast meat was lower in ME 3,150kcal/kg than the ME 3,200kcal/kg (P<0.05). Moisture, shear force and pH were not statistically different among treatments. Protein solubility was increased by increasing ME and CP in diets. The collagen was tended to increase as dietary ME increased. Redness was remarkably higher in ME 3,150kcal/kg than the ME 3,100kcal/kg (P<0.05). Yellowness of meat fed ME 3,150kcal/kg showed significantly lower than other treatments (P<0.05). Therefore, the optium ME and CP to improve the productivity for each period were 3,000kcal/kg, 23%; 3,100kcal/kg, 19%; 3,150kcal/kg, CP 16%, repectively.