• The Korean Journal of Physiology and Pharmacology
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• 제21권6호
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• pp.609-616
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• 2017

Ardipusilloside-I is a natural triterpenoid saponin, which was isolated from Ardisia pusilla A. DC. The aim of the study was to evaluate the stimulation of ardipusilloside-I on gastrointestinal motility in vitro and in vivo. The experiment of smooth muscle contraction directly monitored the contractions of the isolated jejunal segment (IJS) in different contractile states, and the effects of ardipusilloside-I on myosin were measured in the presence of $Ca^{2+}$-calmodulin using the activities of 20 kDa myosin light chain ($MLC_{20}$) phosphorylation and myosin $Mg^{2+}$-ATPase. The effects of ardipusilloside-I on gastro emptying and intestinal transit in constipation-predominant rats were observed, and the MLCK expression in jejuna of constipated rats was determined by western blot. The results showed that, ardipusilloside-I increased the contractility of IJS in a dose-dependent manner and reversed the low contractile state (LCS) of IJS induced by low $Ca^{2+}$, adrenaline, and atropine respectively. There were synergistic effects on contractivity of IJS between ardipusilloside-I and ACh, high $Ca^{2+}$, and histamine, respectively. Ardipusilloside-I could stimulate the phosphorylation of $MLC_{20}$ and $Mg^{2+}$-ATPase activities of $Ca^{2+}$- dependent phosphorylated myosin. Ardipusilloside-I also stimulated the gastric emptying and intestinal transit in normal and constipated rats in vivo, respectively, and increased the MLCK expression in the jejuna of constipation-predominant rats. Briefly, the findings demonstrated that ardipusilloside-I could effectively excite gastrointestinal motility in vitro and in vivo.

• Journal of Gastric Cancer
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• 제22권2호
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• pp.135-144
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• 2022

Purpose: This study aimed to compare the surgical and oncological outcomes between totally laparoscopic pylorus-preserving gastrectomy (TLPPG) with intracorporeal anastomosis and laparoscopy-assisted pylorus-preserving gastrectomy (LAPPG) with extracorporeal anastomosis. Materials and Methods: A retrospective analysis was performed in 258 patients with cT1N0 gastric cancer who underwent laparoscopic pylorus-preserving gastrectomy using two different anastomosis methods: TLPPG with intracorporeal anastomosis (n=88) and LAPPG with extracorporeal anastomosis (n=170). The following variables were compared between the two groups to assess the postoperative surgical and oncological outcomes: proximal and distal margins, number of resected lymph nodes (LNs) in total and in LN station 6, operation time, postoperative hospital stay, and postoperative morbidity including delayed gastric emptying (DGE). Results: The average length of the proximal margin was similar between the TLPPG and LAPPG groups (2.35 vs. 2.73 cm, P=0.070). Although the distal margin was significantly shorter in the TLPPG group than in the LAPPG group (3.15 vs. 4.08 cm, P=0.001), no proximal or distal resection margin-positive cases were reported in either group. The average number of resected LN was similar in both groups (36.0 vs. 33.98, P=0.229; LN station 6, 5.72 vs. 5.33, P=0.399). The operation time was shorter in the TLPPG group than in the LAPPG (200.17 vs. 220.80 minutes, P=0.001). No significant differences were observed between the two groups in terms of postoperative hospital stay (9.38 vs. 10.10 days, P=0.426) and surgical complication rate (19.3% vs. 22.9%), including DGE (8.0% vs. 11.8%, P=0.343). Conclusions: The oncological safety and postoperative complications of TLPPG with intracorporeal anastomosis are similar to those of LAPPG with extracorporeal anastomosis.

• 대한핵의학회지
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• 제39권4호
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• pp.257-262
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• 2005

목적 : 위배출신티그래피는 일반적으로 15분 간격으로 2시간 동안 촬영된다. 본 연구는 수학적 함수를 이용하여 90분까지 얻은 위잔류율 측정치를 가지고 비선형 곡선을 구한 후, 120분의 위잔류율을 예측함으로써 90분 이후의 지연영상 촬영시간을 절약할 수 있는지에 대하여 알아보았다. 대상 및 방법 : 환자들은 74 MBq (2 mCi) Tc-99m DTPA가 들어 있는 계란찜을 섭취한 직후 촬영을 시행하였다. 환자들을 반위배출시간에 따라 두 군으로 나누어 후향적으로 연구하였다. Group I은 반위배출시간이 90분 이하($T_{1/2}\;{\leq}90\;min$)인 51명(남자 21명, 여자: 30명, 평균나이: $44.6{\pm}13.5$세)이었고, Group II는 90분 초과 120분 이하($90\;min)인 45명(남자: 15, 여자:30, 평균나이 $45.6{\pm}15.9$)이었다. 0, 15, 30, 45, 60, 75, 90분에서의 위잔류율을 측정하여 비선형 곡선인 단순지수함수, power exponential function, modified power exponential function을 구하였고, 또한 50, 75, 90분의 측정치로 후기 단순지수함수를 구하였다($MATLAB^{\circledR}$ 5.3). 얻어진 함수식을 통해 120분에서의 위잔류율 예측치를 구한 후, 120분 측정치와의 상관관계를 알아보았다. ($MedCalc^{\circledR}$ 6.0). 결과: 120분 위잔류율의 측정치와 예측치의 상관계수(r)는 각각의 함수식에 따라 다음과 같다; 단순지수함수 (Group I: 0.8858, Group II: 0.5982, p<0.0001), power exponential function (Group I: 0.8755, Group II: 0.6008, p<0.0001), modified power exponential function (Group I: 0.8892, Group II: 0.5882, p<0.0001), 후기 단순지수함수 (Group I:0.9085, Group II:0.6832, p<0.0001). Group I에서만 함수식 모두에서 측정치와 예측치 간에 통계학적으로 의미있게 강한 상관관계를 보였다. 각 상관계수 간에 유의한 차이는 없었으나 후기지수함수식이 다른 함수식에 비하여 변수(parameter)와 입력될 측정치의 개수가 적어 예측치를 구하기가 간편하였다. 결론: 반위배출 시간이 90분 이하인 경우에 120분에서의 위잔류율을 예측할 수 있어 90분 이후의 촬영시간을 절약할 수 있다. 또한, 후기지수함수식이 다른 함수식에 비하여 예측치를 구하기가 간편하므로 임상적으로 더 유용하다.

• 생명과학회지
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• 제26권11호
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• pp.1253-1258
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• 2016

만삼(Codonopsis pilosula)의 뿌리를 말린 한약재인 당삼은 오래 전부터 우리나라와 중국 등지에서 위장관 기능항진에 전통적으로 쓰여 왔다. 본 연구에서는 당삼의 열수 및 에탄올 추출물을 제조하여 실험하였는데, 우선 마우스 단회 투여 경구 독성 시험 결과, 두 추출물 모두 5 g/kg의 고용량 투여 시에도 급성 독성을 나타내지 않았다. 당삼 추출물이 위장관 운동 기능에 미치는 영향을 검정하기 위해, 마우스에서 in vivo 위 배출 속도 및 위장관 이송률을 측정하는 실험을 수행하였다. 두 추출물 모두 마우스의 위 배출 속도에는 유의적인 영향을 주지 못한데 반해, 당삼 열수 추출물은 정상 및 실험적 위장관 운동 저해 마우스에서 모두 위장관 이송률을 유의적으로 증가시켰으며, 용량 의존적 증가 양상을 보였다. 이상과 같이, 본 연구에서는 당삼 열수 추출물이 정상 및 위장관 운동 기능 저해 상황의 마우스에서 모두 위장관 이송률을 증가시켰음을 최초로 보고하였으며, 이는 당삼 열수 추출물이 잠재적으로 인간의 위장관 운동 기능 저해 상황을 개선하는 신약 또는 건강 보조제로 개발될 수 있는 후보 물질임을 시사한다.

• Clinical and Experimental Pediatrics
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• 제48권4호
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• pp.433-437
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• 2005

위석은 비교적 흔하지 않은 질환이지만, 진단을 못할 경우 위궤양과 출혈, 장천공, 장중첩증, 장폐쇄, 복막염 등의 심각한 합병증을 동반할 수 있는 만성적 복통의 한 원인이며, 모발석 환아의 대부분은 발모벽과 식모증의 과거력이 있다. 저자들은 내원 4년 전까지 자기 머리카락을 뽑아서 먹는 습관이 있었고, 이후 간헐적 복통과 오심, 구토를 일으킨 11세 여아에서 모발석을 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• 한국임상약학회지
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• 제20권2호
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• pp.128-137
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• 2010

This review summarizes gender differences in pharmacokinetics, pharmacodynamics, and adverse drug reactions. Gender differences in pharmacokinetics are categorized by four major factors: absorption/bioavailability, distribution, metabolism, and elimination. There are sex-based differences in gastric emptying time, gastric alcohol dehydrogenase activity, apparent volume of distribution, ${\alpha}1$-acid glycoprotein level, phase I (CYP) and phase II metabolizing enzymes, glomerular filtration rate, and drug transporters. This review also reports gender differences in pharmacokinetics and pharmacodynamics of cardiovascular agents, central nervous system acting agents and antiviral agents. In addition, it has been reported that females experience more adverse reactions such as coughing, tachycardia, nausea, vomiting, rash, hypersensitivity, hepatotoxicity, and metabolic disorder after taking cardiovascular, central nervous system acting and antiviral agents. Therefore, in order to provide optimal drug dosage regimens both in male and female, gender differences in pharmacokinetics, pharmacodynamics, and adverse drug reactions must be considered.

• 약학회지
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• 제36권3호
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• pp.212-219
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• 1992

Although oral route is the most convenient route for drug administration, the short and variable transit of drug through GI tract restricts the sustained drug absorption after oral administration. Thus, for sustained absorption of drugs, it is desirable to prolong the GI transit time by retaining the dosage forms in the stomach. In this study, the enzyme-digestible swelling hydrogel was synthesized by heating the mixed solution of N-vinyl-2-pyrrolidone[monomer], acrylated albumin[crosslinking agent] and proxyphylline[drug] at $65^{\circ}C$ for 10 hours in the cylindrical test tube. The resultant hydrogel tablet (diameter; 0.77 cm, thickness; 0.47 cm) was designed to swell in the gastric fluid after oral administration to such a size that passing through the pylorus could be inhibited during the drug release. After releasing drug, the hydrogel was expected to be degraded by pepsin, an enzyme in the stomach, and eventually solubilized. Actually, the hydrogel synthesized in the study swelled to a size larger than the diameter of the pylorus ($1.3{\pm}0.7$ cm) and slowly digested in the presence of pepsin. Drug release from the hydrogel was prolonged up to about 12 hours. The swelling kinetics was dependent on albumin acrylation time, drug content and gel thickness. Particularly the gel thickness was the most important factor that influences on drug release. By adjusting these factors, the albumin-crosslinked hydrogel was expected to be retained in the stomach for up to 60 hours and used as a potential platform of drugs for long-term GI absorption.

• 대한한방내과학회지
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• 제26권1호
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• pp.265-274
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• 2005

Diabetic gastroparesis is a disorder in a diabetic of delayed gastric emptying in the absence of mechanical obstruction. It is estimated that about 20%-50% of diabetics suffer from gastroparesis. Clinical Sympoms include early satiety, bloating, nausea, anorexia, vomiting, abdominal pain, and weight loss. Severe gastroparesis might result in recurrent hospitalization, malnutrition, and even death, but, no exact treatment has yet been established. Electrogastrography has been known to be a simple, non-invasive, and effective method in assessing gastric motility and EGG has been used to diagnosis diabetic gastroparesis and to estimate its prognosis. Recently, two cases of diabetic gastroparesis were observed. For this cases, manual acupucture, electroacupucture, lumbar skin warming and herb medicine were applied. After application of these therapies, gastrointestinal symptoms improved and these therapeutic effects were confirmed in EGG. Moreover, blood glucose control improved, so patients were able to discontinue insulin injection and change to p.o.medication. In light of this encouraging application of oriental medicine, this is reported along with investigation of the literature.

• 대한지역사회영양학회지
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• 제1권2호
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• pp.260-268
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• 1996

This study aimed to develop an experimental animal model of duodenal ulcer for application to nutrition, and to verify the nutritional characteristics. The 7 different doses and 8 different injections of cysteamine and 2 kinds of diets with different casein levels(10%, 20%) were tested to examine the incidence and the process of duodenal ulcer in female Sprague-Dawley rats. The result showed that the duodenal ulcer was induced when rats fed 10% casein diet(for 9 days) were injected 6 times with 13mg/100g BW cysteamine. The first injection was conducted after 24 hours of fasting, followed by the 2nd shot after 3 hours. The following shots were repeated every 3 day(4th and 7th days). Duodenal ulcer was observed 3 days after the last injection by optical microscope and the naked eye. The characteristics of nitrogen bioavailability of duodenal ulcer model were as followings : 1) gastric emptying rate was faster, 2) trypsin activity at duodenum was higher, 3) total protein concentration at serum and nitrogen retention rate were lower than the control. (Korean J Community Nutrition 1(2) : 260-268, 1996)

• 생물정신의학
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• 제3권2호
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• pp.149-155
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• 1996

Doctors who treat pregnant women ore usually cautious in writing their prescription for the drugs. The problem of which psychotropic medications ore sale during pregnancy seems to remain unsolved for many years. Although the rate of absorption is reduced due to a reduced rate of gastric emptying, the extent of absorption of drug is generally unchanged during pregnancy. Plasma volume and total body water increase during pregnancy. There is suggestion that drug metabolizing activity may be increased in pregnancy. Since the pregnancy increase the glomerular filtration rate significantly, drugs mainly eliminated by renal excretion will be cleared more quickly. Factors contributing to the potential teratogenecity of a drug include the type of compound, dose and duration of use, developmental stage of fetus at the time of exposure, and the effect of the drug on fetal pharmacokinetics. All major classes of psychotropic agents should be assumed to diffuse readily across the placenta to the fetus and to be present in some quantity in the breast milk. To decide when and how to start the drug treatment depends on an assessment of the risks related both with and without drug treatment of psychiatric disorders.