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Effects of the Dried Root of Codonopsis pilosula on Gastrointestinal Motor Function in Mice

당삼이 마우스 위장관 운동 기능에 미치는 영향

  • Kim, Min Woo (Department of Life Science & Biotechnology, Dong-eui University) ;
  • Kim, Hyun Jin (Department of Life Science & Biotechnology, Dong-eui University) ;
  • Kim, Moon-Moo (Department of Chemistry, Dong-eui University) ;
  • Lee, Eun-Woo (Department of Life Science & Biotechnology, Dong-eui University) ;
  • Kwon, Hyun Ju (Department of Life Science & Biotechnology, Dong-eui University) ;
  • Kim, Byung Woo (Department of Life Science & Biotechnology, Dong-eui University) ;
  • Lee, Hyun-Tai (Department of Life Science & Biotechnology, Dong-eui University)
  • Received : 2016.09.12
  • Accepted : 2016.10.11
  • Published : 2016.11.30

Abstract

Although major advances have been achieved in our understanding and treatment of diseases in many areas of medicine, relatively few improvements have been made in the area of gastrointestinal (GI) motor function. The dried root of Codonopsis pilosula (Franch.) Nannf. (CP) has been used as a traditional folk medicine for improving poor GI function in East Asia, including China and Korea. In the present study, neither aqueous (CP-W) nor ethanolic (CP-E) extracts of CP showed significant toxicity, even at an oral dose of 5 g/kg to mice. The effects of CP-W and CP-E on GI motor function were investigated by measuring in vivo the gastric emptying rate (GER) and intestinal transit rate (ITR) in mice. In normal mice, the ITR was significantly increased by CP-W in a dose-dependent manner, whereas the GER was not significantly affected by any CP extracts. The ITR was significantly retarded in the mice with experimental GI motility dysfunction (i.e., peritoneal irritation by acetic acid) compared with that in normal mice. However, the retardation was significantly recovered by the pre-treatment of CP-W in a dose-dependent manner. The above results suggest that CP-W might be a potential prokinetic agent preventing or alleviating GI motility dysfunctions in human patients.

만삼(Codonopsis pilosula)의 뿌리를 말린 한약재인 당삼은 오래 전부터 우리나라와 중국 등지에서 위장관 기능항진에 전통적으로 쓰여 왔다. 본 연구에서는 당삼의 열수 및 에탄올 추출물을 제조하여 실험하였는데, 우선 마우스 단회 투여 경구 독성 시험 결과, 두 추출물 모두 5 g/kg의 고용량 투여 시에도 급성 독성을 나타내지 않았다. 당삼 추출물이 위장관 운동 기능에 미치는 영향을 검정하기 위해, 마우스에서 in vivo 위 배출 속도 및 위장관 이송률을 측정하는 실험을 수행하였다. 두 추출물 모두 마우스의 위 배출 속도에는 유의적인 영향을 주지 못한데 반해, 당삼 열수 추출물은 정상 및 실험적 위장관 운동 저해 마우스에서 모두 위장관 이송률을 유의적으로 증가시켰으며, 용량 의존적 증가 양상을 보였다. 이상과 같이, 본 연구에서는 당삼 열수 추출물이 정상 및 위장관 운동 기능 저해 상황의 마우스에서 모두 위장관 이송률을 증가시켰음을 최초로 보고하였으며, 이는 당삼 열수 추출물이 잠재적으로 인간의 위장관 운동 기능 저해 상황을 개선하는 신약 또는 건강 보조제로 개발될 수 있는 후보 물질임을 시사한다.

Keywords

References

  1. Chen, H. T., Tsai, Y. L., Chen, Y. S., Jong, G. P., Chen, W. K., Wang, H. L., Tsai, F. J., Tsai, C. H., Lai, T. Y., Tzang, B. S., Huang, C. Y. and Lu, C. Y. 2010. Dangshen (Codonopsis pilosula) activates IGF-I and FGF-2 pathways to induce proliferation and migration effects in RSC96 Schwann cells. Am. J. Chin. Med. 38, 359-372. https://doi.org/10.1142/S0192415X10007907
  2. He, J. Y., Ma, N., Zhu, S., Komatsu, K., Li, Z. Y. and Fu, W. M. 2015. The genus Codonopsis (Campanulaceae): a review of phytochemistry, bioactivity and quality control. J. Nat. Med. 69, 1-21. https://doi.org/10.1007/s11418-014-0861-9
  3. He, K., Li, X., Chen, X., Ye, X., Huang, J., Jin, Y., Li, P., Deng, Y., Jin, Q., Shi, Q. and Shu, H. 2011. Evaluation of antidiabetic potential of selected traditional Chinese medicines in STZ-induced diabetic mice. J. Ethnopharmacol. 137, 1135-1142. https://doi.org/10.1016/j.jep.2011.07.033
  4. Kang, S. Y., Jung, J. K., Lee, S. K., Lee, S. H. and Park, Y. K. 2013. Effects of the ethanol extract of Codonopsis Pilosulae Radix on ovalbumin-induced allergic responses in mice. Kor. J. Herbology 28, 9-15.
  5. Lee, H. T. 2014. Prokinetic activity of ethanolic extracts from dried Citrus unshiu peels in mice. J. Life Sci. 24, 260-265. https://doi.org/10.5352/JLS.2014.24.3.260
  6. Lee, H. T., Seo, E. K., Chung, S. J. and Shim, C. K. 2005. Prokinetic activity of an aqueous extract from dried immature fruit of Poncirus trifoliata (L.) Raf. J. Ethnopharmacol. 102, 131-136. https://doi.org/10.1016/j.jep.2005.05.052
  7. Ryu, H. Y., Ahn, S. M., Kim, J. S. and Sohn, H. Y. 2010. Evaluation of in-vitro anticoagulation activity of 33 different medicinal herbs. J. Life Sci. 20, 922-928. https://doi.org/10.5352/JLS.2010.20.6.922
  8. Ryu, J. H. and Lee, H. T. 2013. Effects of dried Citrus unshiu peels on gastrointestinal motility in rodents. Arch. Pharm. Res. 36, 641-648. https://doi.org/10.1007/s12272-013-0080-z
  9. Sanders, K. M., Ward, S. M. and Koh, S. D. 2014. Interstitial cells: regulators of smooth muscle function. Physiol. Rev. 94, 859-907. https://doi.org/10.1152/physrev.00037.2013
  10. Suchitra, A. D., Dkhar, S. A., Shewade, D. G. and Shashindran, C. H. 2003 Relative efficacy of some prokinetic drugs in morphine-induced gastrointestinal transit delay in mice. World J. Gastroenterol. 9, 779-783. https://doi.org/10.3748/wjg.v9.i4.779
  11. Sun, Y. X. 2009. Immunological adjuvant effect of a watersoluble polysaccharide, CPP, from the roots of Codonopsis pilosula on the immune responses to ovalbumin in mice. Chem. Biodivers. 6, 890-896. https://doi.org/10.1002/cbdv.200800154
  12. Wang, Z. T., Du, Q., Xu, G. J., Wang, R. J., Fu, D. Z. and Ng, T. B. 1997. Investigations on the protective action of Condonopsis pilosula (Dangshen) extract on experimentallyinduced gastric ulcer in rats. Gen. Pharmacol. 28, 469-473. https://doi.org/10.1016/S0306-3623(96)00047-X
  13. Xu, C., Liu, Y., Yuan, G. and Guan, M. 2012. The contribution of side chains to antitumor activity of a polysaccharide from Codonopsis pilosula. Int. J. Biol. Macromol. 50, 891-894. https://doi.org/10.1016/j.ijbiomac.2012.01.013
  14. Zeinali, F., Stulberg, J. J. and Delaney, C. P. 2009. Pharmacological management of postoperative ileus. Can. J. Surg. 52, 153-157.
  15. Zheng, T. Z., Li, W., Qu, S. Y., Ma, Y. M., Ding, Y. H. and Wei, Y. L. 1998. Effects of Dangshen on isolated gastric muscle strips in rats. World J. Gastroenterol. 4, 354-356. https://doi.org/10.3748/wjg.v4.i4.354