• 제목/요약/키워드: Elfvingia applanata (EA)

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Elfvingia applanata 수용성 물질의 항엔세파로미오카디티스 바이러스작용과 인터페론과의 병용효과 (Anti-encephalomyocarditis Virus Activity of Water Soluble Substance from Elfvingia applanata Alone and in Combinations with Interferons)

  • 김준희;어성국;김영소;한성순
    • 약학회지
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    • 제43권4호
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    • pp.464-468
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    • 1999
  • In order to find less toxic antiviral agents from basidiomycetes, EA, the water soluble substance, was isolated from the carpophores of Elfvingia applanata (Pers.) Karst. Anti-encephalomyocarditis (EMC) virus activity of EA was examined in Vero cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha and gamma were examined on the multiplication of EMC virus. EA exhibited a concentration-dependent reduction in the plaque formation of EMC virus with 50% effective concentration ($EC_{50}$) of 2.12 mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha showed potent synergism with CI values of 0.40~0.60 for 50%, 70% and 90% effective levels, but that with IFN gamma showed antagonism with CI values of 2.16~2.83.

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Elfvingia applanata 엑스가 수종 항생물질의 항균력에 미치는 병용효과 (Antimicrobial Activity of Elfvingia applanata extract alone and in Combination with Some Antibiotics)

  • 김영소;임교환;이종길;한성순
    • 약학회지
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    • 제38권6호
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    • pp.742-748
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    • 1994
  • As part of our search for less toxic antimicrobial agents from natural resources, the carpophores of Elfvingia applanata$(P_{ers}.)K_{ARST}.$ was extracted with hot water. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. Antimicrobial activity of EA was tested in vitro against Gram positive and Gram negative bacteria by serial broth dilution method, and the antimicrobial activity was expressed by minimal inhibitory concentration(MIC). Among fourteen species of bacteria tested, the antimicrobial activity of EA was the most potent against Proteus vulgaris showing MIC of 1.250 mg/ml. To investigate the effect of antimicrobial combinations of EA with four kinds of antibiotics(ampicillin, cefazolin, oxytetracycline and chloramphenicol), the fractional inhibitory concentration index(FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of EA with four kinds of antibiotics resulted in synergism in four instances, but no antagonism was observed. Four instances of synergism were observed when EA was combined with ampicillin against Micrococcus luteus, with cefazolin against Bacillus subtilis, with cefazolin against Micrococcus luteus and with oxytetracycline against Staphylococcus aureus.

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잔나비걸상버섯 수용성물질의 항인플루엔자바이러스 작용과 인터페론과의 병용효과 (Anti-influenza Virus Activity of Water Soluble Substance from Elfvingia applanata Alone and in Combinations with Interferons)

  • 정선식;어성국;김영소;한성순
    • 약학회지
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    • 제43권4호
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    • pp.469-473
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    • 1999
  • EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers). Karst. Anti-influenza A virus (anti-Flu A) activity of EA was examined of Vero cells by plaque reduction assay in vitro. And the combined antiviral effects fo EA with interferon (IFN) alpha and gamma were examined on the multiplication of Flu A with 50% effective concentration ($EC_50$) of 1.50 mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha on Flu A showed more potent synergism with CI values of 0.50~0.52 of 50%, 70%, 90% effective levels than that with IFN gamma with CI values of 0.82~0.99.

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잔나비걸상 엑스의 생쥐 급성독성에 미치는 영향 (Effect of Elfvingia applanata extract on the Acute Toxicity in Mice)

  • 김영소;강종구;이종길;한성순
    • 약학회지
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    • 제38권6호
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    • pp.756-762
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    • 1994
  • To investigate the acute toxicity of Elfvingia applanata$(P_{ers})K_{ARST}$, it was extracted with hot water. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. The acute toxicity of EA was investigated in ICR mice of both sexes. Five groups of mice were administered orally and intraperitoneally with 375, 750, 1,500, 3,000, 6,000 mg/kg(p.o.) and 312.5, 625, 1,250, 2,500, 5,000 mg/kg (i.p.) of EA Abnormal clinical signs related to EA were not observed and no deaths occurred. $LD_{50}$ values administered orally and intraperitoneally were evaluated to be over 6,000 mg/kg and over 5,000 mg/kg, respectively. Gross finding of necropsy revealed no evidence of specific toxicity related to EA. There were no significant changes in body weights, serum biochemical values and histopathological changes compared with control group.

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Anti-Varicella Zoster Virus Activity of Water Soluble Substance from Elfvingia applanata Alone and in Combinations with Acyclovir and Vidarabine

  • Kim, Soo-Dong;Eo, Seong-Kug;Kim, Young-So;Han, Seong-Sun
    • Natural Product Sciences
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    • 제5권2호
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    • pp.107-111
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    • 1999
  • To investigate less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was isolated from the carpophores of Elfvingia applanata (pers.) Karst. Anti-varicella zoster virus (Oka strain; anti-VZV/Oka) activity of EA was examined in MRC-5 cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with nucleoside anti-VZV agents, acyclovir and vidarabine, were examined on the multiplication of VZV/Oka. EA exhibited a concentration-dependent reduction in the plaque formation of VZV/Oka with a 50% effective concentration $(EC_{50})$ of $464.14\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combination of EA with acyclovir showed more potent synergism with CI values of $0.18{\sim}0.62$ for $50{\sim}90%$ effective levels than that of EA with vidarabine with CI values of $0.67{\sim}1.04$.

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잔나비걸상버섯 수용성물질의 항바이러스효과 (Antiviral Activity of Water Soluble Substance from Elfvingia applanata)

  • 임교환;어성국;김영소;이종길;한성순
    • 생약학회지
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    • 제30권1호
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    • pp.25-33
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    • 1999
  • In order to find less toxic antiviral agents from basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers.) Karst. EA was examined for antiviral activity against five strains of pathogenic viruses such as encephalomyocarditis virus (EMCV), vesicular stomatitis virus (VSV) Indiana and New Jersey strains, influenza A virus (Flu A), and varicella zoster virus (VZV) in vitro. Antiviral activity was evaluated by plaque reduction assay. Among five strains of viruses tested, EA exhibited the most potent antiviral activity against VSV Indiana strain with 50% effective concentration $(EC_{50})$ of 0.104 mg/ml in Vero cells, and its selectivity index (SI) was 36.5. EA was also examined for the virucidal activity, antiviral activity in preincubation on VSV Indiana strain in order to examine possible mode of antiviral activity. Preincubation of Vero cells with EA did not confer protection against VSV, however, prolonged exposure of cells to EA inhibited the replication of virus dose-dependently. In virucidal activity, the titer of infectious virus did not decrease significantly.

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잔나비걸상 수용성 물질의 Vesicular Stomatitis Virus (Indiana Serotype)에 대한 항바이러스작용과 Interferon과의 병용효과 (Antiviral Effect of Water Soluble Substance from Elfvingia applanata Alone and in Combinations with Interferons on Vesicular Stomatitis Virus (Indiana Serotype))

  • 김영소;어성국;한성순
    • 생약학회지
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    • 제30권3호
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    • pp.244-249
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    • 1999
  • In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata(Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [Indiana serotype, VSV(IND)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or gamma were examined on the multiplication of VSV(IND). EA caused a concentration-dependent reduction in the plaque formation of VSV(IND) with 50% effective concentration $(EC_{50})$ of $104.02\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. All cases of the combination of EA with IFN alpha or IFN gamma showed potent synergism with CI values of $0.38{\sim}0.52$ for $50{\sim}90%$ effective levels.

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잔나비걸상 수용성물질의 Vesicular Stomatitis Virus(New Jersey Serotype)에 대한 항바이러스작용과 Interferon과의 병용효과 (Antiviral Effect of Water Soluble Substance from Elfvingia applanata Alone and in Combinations with Interferons Against Vesicular Stomatitis Virus (New Jersey Serotype))

  • 임교환;어성국;김영소;임재윤;한성순
    • 한국균학회지
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    • 제27권2호통권89호
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    • pp.175-179
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    • 1999
  • 잔나비걸상 Elfvingia applananta 자실체의 수용성물질 EA의 vesicular stomatitis virus[New Jersey serotype, VSV(NJ)]에 대한 항바이러스효과를 plaque reduction assay에 따라 실험한 결과 EA는 용량의존적으로 plaque 형성을 억제하였으며 $EC_{50}$는 2.10 mg/ml이었다. 단백질성 항바이러스제인 interferon(IFN)과 EA와의 병용시험 결과 f(a)가 0.50에서 0.90인 유효농도범위에서 IFN alpha와 병용시 f(a)의 값이 커짐에 따라 상가작용 내지는 길항작용을 나타내었으며, IFN gamma와의 병용시에는 길항작용을 나타내었다. 따라서 IFN alpha와의 병용시 f(a)가 0.50내지 0.70의 유효농도 범위에서 상가효과가 있다.

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Antibacterial Activity of Water Soluble Components of Elfvingia applanata Alone and in Combinations with Quinolones

  • Kim, Young-So;Eo, Seong-Kug;Oh, Ki-Wan;Lee, Chong-Kil;Lee, Young-Nam;Han, Seong-Sun
    • Mycobiology
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    • 제29권1호
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    • pp.11-14
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    • 2001
  • A preparation of water soluble components(EA) was made from carpophores of Elfvingia applanata(Pers.) Karst and its in vitro antibacterial activity on a number of bacterial species was examined by macrobroth dilution assay. Among 16 species of bacteria tested, the most potent antibacterial activity was observed against Staphylococcus epiderrnidis and Proteus vulgaris, of which MICs were 1.25 mg/ml. To investigate the antibacterial effects in combinations of EA with quinolone antibiotics, such as ciprofloxacin, enoxacin, lomefloxacin, norfloxacin, and ofloxacin, the fractional inhibitory concentrations(FICs) and the fractional inhibitory concentration indices(FICIs) for four bacterial strains were determined by macrobroth dilution checkerboard assay. Combinations of EA and quinolones exhibited either additive or indifferent effects of antibacterial activity in most instances. However, both synergistic and antagonistic effects were not observed in any cases.

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Mode of Antiviral Activity of Water Soluble Components Isolated from Elfvingia applanata on Vesicular Stomatitis Virus

  • Eo, Seong-Kug;Kim, Young-So;Oh, Ki-Wan;Lee, Chong-Kil;Lee, Young-Nam;Han, Seong-Sun
    • Archives of Pharmacal Research
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    • 제24권1호
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    • pp.74-78
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    • 2001
  • A preparation of water soluble components (EA) was made from carpophores of Elfvingia applanata (Pers.) Karst and its in vitro antiviral activity on vesicular stomatitis virus [(Indiana serotype, VSV(IND)] was investigated by plaque reduction assay. EA exhibited potent antiviral activity on VSV(IND) growth and negligible cytotoxicity on Vero cells, 50% effective concentration ($EC_{50}C$/) of 104$ug\textrm\/ml$ and 50% cytotoxic concentration ($CC_{50}C$) of 3,793$ug\textrm\/ml$, respectively. Selectivity index (Sl $CC_{50}C$/$EC_{50}C$) of EA on Vero cell and VSV(IND) was about 36.5. EA did not display either a direct virucidal effect on V5V(IND) or induction of antiviral substance by Vero cells upon its treatment. Thus, the mode of antiviral activity of EA was studied at steps of viral adsorption onto cell. When both EA and virus were added to cell monolayers, titer of cell-free virus in culture supernatant increased in ca. 30-40% compared with that of control group and titer of cell-associated virus was 60-100% higher than that of control group. These results suggested that antiviral activity of EA on VSV(IND) might be due to the hindrance of viral entry to cells at eITher endocytosis or loss of envelope.

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